Abstract：
:To date, analyses of individual targets have provided evidence of a miRNA targetome that extends beyond the boundaries of messenger RNAs (mRNAs) and can involve non-Watson-Crick base pairing in the miRNA seed region. Here we report our findings from analyzing 34 Argonaute HITS-CLIP datasets from several human and mouse cell types. Investigation of the architectural (i.e. bulge vs. contiguous pairs) and sequence (Watson-Crick vs. G:U pairs) preferences for human and mouse miRNAs revealed that many heteroduplexes are "non-canonical" i.e. their seed region comprises G:U and bulge combinations. The genomic distribution of miRNA targets differed distinctly across cell types but remained congruent across biological replicates of the same cell type. For some cell types intergenic and intronic targets were more frequent whereas in other cell types mRNA targets prevailed. The findings suggest an expanded model of miRNA targeting that is more frequent than the standard model currently in use. Lastly, our analyses of data from different cell types and laboratories revealed consistent Ago-loaded miRNA profiles across replicates whereas, unexpectedly, the Ago-loaded targets exhibited a much more dynamic behavior across biological replicates.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Clark PM,Loher P,Quann K,Brody J,Londin ER,Rigoutsos Idoi
10.1038/srep05947subject
Has Abstractpub_date
2014-08-08 00:00:00pages
5947issn
2045-2322pii
srep05947journal_volume
4pub_type
杂志文章abstract：:Although various drugs, environmental pollutants and nanoparticles (NP) can cross the human placental barrier and may harm the developing fetus, knowledge on predictive placental transfer rates and the underlying transport pathways is mostly lacking. Current available in vitro placental transfer models are often inapp...
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pub_type： 杂志文章
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pub_type： 已发布勘误
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pub_type： 杂志文章
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pub_type： 杂志文章,meta分析,评审
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更新日期：2016-08-19 00:00:00
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