Abstract:
:Diatoms, a major group of photosynthetic microalgae, have a high biotechnological potential that has not been fully exploited because of the paucity of available genetic tools. Here we demonstrate targeted and stable modifications of the genome of the marine diatom Phaeodactylum tricornutum, using both meganucleases and TALE nucleases. When nuclease-encoding constructs are co-transformed with a selectable marker, high frequencies of genome modifications are readily attained with 56 and 27% of the colonies exhibiting targeted mutagenesis or targeted gene insertion, respectively. The generation of an enhanced lipid-producing strain (45-fold increase in triacylglycerol accumulation) through the disruption of the UDP-glucose pyrophosphorylase gene exemplifies the power of genome engineering to harness diatoms for biofuel production.
journal_name
Nat Communjournal_title
Nature communicationsauthors
Daboussi F,Leduc S,Maréchal A,Dubois G,Guyot V,Perez-Michaut C,Amato A,Falciatore A,Juillerat A,Beurdeley M,Voytas DF,Cavarec L,Duchateau Pdoi
10.1038/ncomms4831subject
Has Abstractpub_date
2014-05-29 00:00:00pages
3831issn
2041-1723pii
ncomms4831journal_volume
5pub_type
杂志文章abstract::Frequency tuning within the auditory papilla of most non-mammalian species is electrical, deriving from ion-channel resonance within their sensory hair cells. In contrast, tuning within the mammalian cochlea is mechanical, stemming from active mechanisms within outer hair cells that amplify the basilar membrane travel...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms13133
更新日期:2016-10-31 00:00:00
abstract::In November 2013, a series of earthquakes began along a mapped ancient fault system near Azle, Texas. Here we assess whether it is plausible that human activity caused these earthquakes. Analysis of both lake and groundwater variations near Azle shows that no significant stress changes were associated with the shallow...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms7728
更新日期:2015-04-21 00:00:00
abstract::Cystic fibrosis (CF) is a genetic disorder caused by defective CF Transmembrane Conductance Regulator (CFTR) function. Insulin producing pancreatic islets are located in close proximity to the pancreatic duct and there is a possibility of impaired cell-cell signaling between pancreatic ductal epithelial cells (PDECs) ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-11178-w
更新日期:2019-07-16 00:00:00
abstract::Liquid crystalline phases of matter permeate nature and technology, with examples ranging from cell membranes to liquid-crystal displays. Remarkably, electronic liquid-crystal phases can exist in two-dimensional electron systems (2DES) at half Landau-level filling in the quantum Hall regime. Theory has predicted the e...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-017-01810-y
更新日期:2017-11-16 00:00:00
abstract::Multiciliated cells (MCCs) harbor dozens to hundreds of motile cilia, which generate hydrodynamic forces important in animal physiology. In vertebrates, MCC differentiation involves massive centriole production by poorly characterized structures called deuterosomes. Here, single-cell RNA sequencing reveals that human ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-06768-z
更新日期:2018-11-07 00:00:00
abstract::Spin-based devices offer non-volatile, scalable, low power and reprogrammable functionality for emerging device technologies. Here we fabricate nanoscale spintronic devices with ferromagnetic metal/single-layer graphene tunnel barriers used to generate spin accumulation and spin currents in a silicon nanowire transpor...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms8541
更新日期:2015-06-19 00:00:00
abstract::Collisions between the DNA replication machinery and co-transcriptional R-loops can impede DNA synthesis and are a major source of genomic instability in cancer cells. How cancer cells deal with R-loops to proliferate is poorly understood. Here we show that the ATP-dependent chromatin remodelling INO80 complex promote...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-18306-x
更新日期:2020-09-10 00:00:00
abstract::Selective reduction of ketone/aldehydes to alcohols is of great importance in green chemistry and chemical engineering. Highly efficient catalysts are still demanded to work under mild conditions, especially at room temperature. Here we present a synergistic function of single-atom palladium (Pd1) and nanoparticles (P...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-13941-5
更新日期:2020-01-07 00:00:00
abstract::Recent advances in the understanding of spin orbital effects in ultrathin magnetic heterostructures have opened new paradigms to control magnetic moments electrically. The Dzyaloshinskii-Moriya interaction (DMI) is said to play a key role in forming a Néel-type domain wall that can be driven by the spin Hall torque. H...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms5655
更新日期:2014-08-18 00:00:00
abstract::The inheritance of the centrosome during human fertilization remains mysterious. Here we show that the sperm centrosome contains, in addition to the known typical barrel-shaped centriole (the proximal centriole, PC), a surrounding matrix (pericentriolar material, PCM), and an atypical centriole (distal centriole, DC) ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-04678-8
更新日期:2018-06-07 00:00:00
abstract::Under hypoxia, most of glucose is converted to secretory lactate, which leads to the overuse of glutamine-carbon. However, under such a condition how glutamine nitrogen is disposed to avoid over-accumulating ammonia remains to be determined. Here we identify a metabolic flux of glutamine to secretory dihydroorotate, w...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-08033-9
更新日期:2019-01-14 00:00:00
abstract::Graphene flakes with giant shape anisotropy are extensively used to establish connectedness electrical percolation in various heterogeneous systems. However, the percolation behaviour of graphene flakes has been recently predicted to be far more complicated than generally anticipated on the basis of excluded volume ar...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms9700
更新日期:2015-11-16 00:00:00
abstract::Single-atom catalysts have recently been applied in many applications such as CO oxidation. Experimental in situ investigations into this reaction, however, are limited. Hereby, we present a suite of operando/in situ spectroscopic experiments for structurally well-defined atomically dispersed Rh on phosphotungstic aci...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-09188-9
更新日期:2019-03-22 00:00:00
abstract::Standard units of measurement are required for the quantitative description of nature; however, few standard units have been established for genomics to date. Here, we have developed a synthetic DNA ladder that defines a quantitative standard unit that can measure DNA sequence abundance within a next-generation sequen...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-17445-5
更新日期:2020-07-17 00:00:00
abstract::Viscous electron fluids have emerged recently as a new paradigm of strongly-correlated electron transport in solids. Here we report on a direct observation of the transition to this long-sought-for state of matter in a high-mobility electron system in graphene. Unexpectedly, the electron flow is found to be interactio...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-07004-4
更新日期:2018-10-31 00:00:00
abstract::The extraction of a clear and simple footprint of the structure of large, weighted and directed networks is a general problem that has relevance for many applications. An important example is seen in origin-destination matrices, which contain the complete information on commuting flows, but are difficult to analyze an...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms7007
更新日期:2015-01-21 00:00:00
abstract::Magnetic resonance imaging is an important technique for identifying different types of tissues in a body or spatial information about composite materials. Because temperature is a fundamental parameter reflecting the biological status of the body and individual tissues, it would be helpful to have temperature maps su...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms12415
更新日期:2016-08-09 00:00:00
abstract::The nasal cellular epigenome may serve as biomarker of airway disease and environmental response. Here we collect nasal swabs from the anterior nares of 547 children (mean-age 12.9 y), and measure DNA methylation (DNAm) with the Infinium MethylationEPIC BeadChip. We perform nasal Epigenome-Wide Association analyses (E...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-11058-3
更新日期:2019-07-12 00:00:00
abstract::Myoblast fusion (MF) is required for muscle growth and repair, and its alteration contributes to muscle diseases. The mechanisms governing this process are incompletely understood, and no epigenetic regulator has been previously described. Ash1L is an epigenetic activator belonging to the Trithorax group of proteins a...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-07313-8
更新日期:2018-11-28 00:00:00
abstract::Generating a comprehensive map of molecular interactions in living cells is difficult and great efforts are undertaken to infer molecular interactions from large-scale perturbation experiments. Here, we develop the analytical and numerical tools to quantify the fundamental limits for inferring transcriptional networks...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-017-02489-x
更新日期:2018-01-09 00:00:00
abstract::Melting of the Greenland Ice Sheet is a leading cause of land-ice mass loss and cryosphere-attributed sea level rise. Blooms of pigmented glacier ice algae lower ice albedo and accelerate surface melting in the ice sheet's southwest sector. Although glacier ice algae cause up to 13% of the surface melting in this regi...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-20627-w
更新日期:2021-01-25 00:00:00
abstract::Increased aerobic glycolysis in endothelial cells of atheroprone areas of blood vessels has been hypothesized to drive increased inflammation and lesion burden but direct links remain to be established. Here we show that endothelial cells exposed to disturbed flow in vivo and in vitro exhibit increased levels of prote...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-07132-x
更新日期:2018-11-07 00:00:00
abstract::Artificial photosynthesis is a vibrant field of research aiming at converting abundant, low energy molecules such as water, nitrogen or carbon dioxide into fuels or useful chemicals by means of solar energy input. Photo-electrochemical reduction of carbon dioxide is an appealing strategy, aiming at reducing the greenh...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-17125-4
更新日期:2020-07-13 00:00:00
abstract::Natural killer (NK) cells are lymphocytes with important anti-tumour functions. Cytokine activation of NK cell glycolysis and oxidative phosphorylation (OXPHOS) are essential for robust NK cell responses. However, the mechanisms leading to this metabolic phenotype are unclear. Here we show that the transcription facto...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-04719-2
更新日期:2018-06-14 00:00:00
abstract::The generation of temporal cavity solitons in microresonators results in coherent low-noise optical frequency combs that are critical for applications in spectroscopy, astronomy, navigation or telecommunications. Breather solitons also form an important part of many different classes of nonlinear wave systems, manifes...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms14569
更新日期:2017-02-24 00:00:00
abstract::Chemical clocks are often used as exciting classroom experiments, where an induction time is followed by rapidly changing colours that expose oscillating concentration patterns. This type of reaction belongs to a class of nonlinear chemical kinetics also linked to chaos, wave propagation and Turing patterns. Despite i...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms11832
更新日期:2016-06-10 00:00:00
abstract::5-Fluorouracil (5-FU) remains the first-line treatment for colorectal cancer (CRC). Although 5-FU initially de-bulks the tumor mass, recurrence after chemotherapy is the barrier to effective clinical outcomes for CRC patients. Here, we demonstrate that p53 promotes WNT3 transcription, leading to activation of the WNT/...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-19173-2
更新日期:2020-10-21 00:00:00
abstract::Colloidal quantum dots have emerged as a material platform for low-cost high-performance optoelectronics. At the heart of optoelectronic devices lies the formation of a junction, which requires the intimate contact of n-type and p-type semiconductors. Doping in bulk semiconductors has been largely deployed for many de...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms3981
更新日期:2013-01-01 00:00:00
abstract::The Unc-51 like autophagy activating kinase 1 (ULK1) complex plays a central role in the initiation stage of autophagy. However, the function of ULK1 in the late stage of autophagy is unknown. Here, we report that ULK1, a central kinase of the ULK1 complex involved in autophagy initiation, promotes autophagosome-lysos...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-05449-1
更新日期:2018-08-28 00:00:00
abstract::In the original version of this Article, the four structural depictions of orbital distributions were inadvertently omitted from Figure 5. This has now been corrected in both the HTML and PDF versions of the Article. ...
journal_title:Nature communications
pub_type: 已发布勘误
doi:10.1038/s41467-019-10880-z
更新日期:2019-06-21 00:00:00