Abstract：
:Standard units of measurement are required for the quantitative description of nature; however, few standard units have been established for genomics to date. Here, we have developed a synthetic DNA ladder that defines a quantitative standard unit that can measure DNA sequence abundance within a next-generation sequencing library. The ladder can be spiked into a DNA sample, and act as an internal scale that measures quantitative genetics features. Unlike previous spike-ins, the ladder is encoded within a single molecule, and can be equivalently and independently synthesized by different laboratories. We show how the ladder can measure diverse quantitative features, including human genetic variation and microbial abundance, and also estimate uncertainty due to technical variation and improve normalization between libraries. This ladder provides an independent quantitative unit that can be used with any organism, application or technology, thereby providing a common metric by which genomes can be measured.
journal_name
Nat Communjournal_title
Nature communicationsauthors
Reis ALM,Deveson IW,Wong T,Madala BS,Barker C,Blackburn J,Marcellin E,Mercer TRdoi
10.1038/s41467-020-17445-5subject
Has Abstractpub_date
2020-07-17 00:00:00pages
3609issue
1issn
2041-1723pii
10.1038/s41467-020-17445-5journal_volume
11pub_type
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