Intraspecific chromosomal and genetic polymorphism in Brassica napus L. detected by cytogenetic and molecular markers.

Abstract:

:The application of DNA intercalator 9-aminoacridine allowed us to increase the resolution of chromosome C-banding and DAPI-banding patterns and to investigate chromosomal polymorphism in karyotypes of seven spring and six winter rape varieties. It was shown that the pericentromeric and intercalary C-bands of most of the chromosomes in spring rape were smaller in size and less polymorphic than those of winter rape. More 26S and 5S rDNA sites were found in the winter rape karyotypes than the spring varieties. Separate or colocalized 26S and 5S rDNA sites were revealed on chromosomes 4, 5, 6, 8, 10, 14, 15, 16 and 18. Intervarietal and intravarietal polymorphism of the number and chromosomal localization of rDNA sites were detected. The generalized idiogram of chromosomes of 13 Brassica napus varieties with account of all possibilities of C-banding patterns as well as localization of 26S and 5S rDNA sites were constructed. Polymorphism of the examined molecular and cytogenetic markers as well as the heterozygosis level of FAE1.1 gene controlling erucic acid synthesis in rapeseed was higher in the winter varieties than in the spring ones. The obtained data were in a atisfactory agreement with increased tolerance to environmental stress conditions of winter rape.

journal_name

J Genet

journal_title

Journal of genetics

authors

Amosova AV,Zemtsova LV,Grushetskaya ZE,Samatadze TE,Mozgova GV,Pilyuk YE,Volovik VT,Melnikova NV,Zelenin AV,Lemesh VA,Muravenko OV

doi

10.1007/s12041-014-0351-6

subject

Has Abstract

pub_date

2014-04-01 00:00:00

pages

133-43

issue

1

eissn

0022-1333

issn

0973-7731

journal_volume

93

pub_type

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