Abstract:
AIM:To evaluate the in vitro immunogenic and immunomodulatory properties of induced pluripotent stem cells (iPSCs) compared with bone marrow-derived mesenchymal stromal cells (MSCs). MATERIALS & METHODS:Mouse embryonic fibroblasts (MEFs) were isolated from C3HeB/FeJ and C57BL/6J mice, and reprogrammed to generate iPSCs. Mixed leukocyte reactions were performed using MHC-matched and -mismatched responder leukocytes and stimulator leukocytes, iPSCs or MSCs. To assess immunogenic potential, iPSCs and MSCs were used as stimulator cells for responder leukocytes. To assess immunomodulatory properties, iPSCs and MSCs were cultured in the presence of stimulator and responder leukocytes. MEFs were used as a control. RESULTS:iPSCs had similar immunogenic properties but more potent immunomodulatory effects than MSCs. Co-culture of MHC-mismatched leukocytes with MHC-matched iPSCs resulted in significantly less responder T-cell proliferation than observed for MHC-mismatched leukocytes alone and at more responder leukocyte concentrations than with MSCs. In addition, MHC-mismatched iPSCs significantly reduced responder T-cell proliferation when co-cultured with MHC-mismatched leukocytes, while MHC-mismatched MSCs did not. CONCLUSION:These results provide important information when considering the use of iPSCs in place of MSCs in both regenerative and transplantation medicine.
journal_name
Regen Medjournal_title
Regenerative medicineauthors
Schnabel LV,Abratte CM,Schimenti JC,Felippe MJ,Cassano JM,Southard TL,Cross JA,Fortier LAdoi
10.2217/rme.14.29subject
Has Abstractpub_date
2014-01-01 00:00:00pages
621-35issue
5eissn
1746-0751issn
1746-076Xjournal_volume
9pub_type
杂志文章abstract:AIM:Glucotoxicity obstructs pancreatic differentiation from adult stem cells. The aim was to develop a novel protocol for differentiation of dental pulp stem cells (DPSCs) into pancreatic β cells and determine the effect of H2S on glucotoxicity. MATERIALS & METHODS:DPSCs were differentiated with media containing 5.5 o...
journal_title:Regenerative medicine
pub_type: 杂志文章
doi:10.2217/rme-2016-0142
更新日期:2017-03-01 00:00:00
abstract:AIMS:Murine 3T3 feeder cells are commonly used for stem cell expansion. Although 'feeder-free' systems are being developed for a variety of stem cells including embryonic, the use of feeder cells currently remains optimal for the expansion of epithelial stem cells. In this study, MRC-5, a human embryonic fibroblast cel...
journal_title:Regenerative medicine
pub_type: 杂志文章
doi:10.2217/17460751.2.6.919
更新日期:2007-11-01 00:00:00
abstract::Genetics Policy Institute (GPI) is a nonprofit, public interest organization recognized as a leading promoter and defender of stem cell research and other cutting-edge medical research targeting cures. Since GPI's inception in 2003, its mission has focused on establishing a positive policy, regulatory and societal fra...
journal_title:Regenerative medicine
pub_type: 新闻
doi:10.2217/rme.09.6
更新日期:2009-05-01 00:00:00
abstract::Diabetes mellitus represents a global epidemic affecting over 350 million patients worldwide and projected by the WHO to surpass the 500 million patient mark within the next two decades. Besides Type 1 and Type 2 diabetes mellitus, the study of the endocrine compartment of the pancreas is of great translational intere...
journal_title:Regenerative medicine
pub_type: 杂志文章,评审
doi:10.2217/rme.12.70
更新日期:2012-11-01 00:00:00
abstract::Worldwide, tissue engineering and cellular replacement therapies are at the forefront of the regenerative medicine agenda, and researchers are addressing key diseases, including diabetes, stroke and neurological disorders. It is becoming evident that neurological cell therapy is a necessarily complex endeavor. The bra...
journal_title:Regenerative medicine
pub_type: 杂志文章,评审
doi:10.2217/rme.12.2
更新日期:2012-05-01 00:00:00
abstract::Latest developments in the field of stem cell research and regenerative medicine compiled from publicly available information and press releases from nonacademic institutions in March 2020. ...
journal_title:Regenerative medicine
pub_type: 杂志文章
doi:10.2217/rme-2020-0052
更新日期:2020-07-01 00:00:00
abstract::Dimethyl sulfoxide (DMSO) is the cryoprotectant of choice for most animal cell systems since the early history of cryopreservation. It has been used for decades in many thousands of cell transplants. These treatments would not have taken place without suitable sources of DMSO that enabled stable and safe storage of bo...
journal_title:Regenerative medicine
pub_type: 杂志文章
doi:10.2217/rme-2019-0145
更新日期:2020-03-01 00:00:00
abstract:AIM:Demonstrate that periadventitial delivery of adipose-derived mesenchymal stem cells (ADMSCs) slows aneurysm progression in an established murine elastase-perfusion model of abdominal aortic aneurysm (AAA). MATERIALS & METHODS:AAAs were induced in C57BL/6 mice using porcine elastase. During elastase perfusion, a de...
journal_title:Regenerative medicine
pub_type: 杂志文章
doi:10.2217/rme.14.61
更新日期:2014-01-01 00:00:00
abstract::Aim: To describe the successful treatment of a Baker's cyst in the setting of post-traumatic osteoarthritis using ultrasound-guided injection of platelet-rich plasma. Setting: Outpatient sports clinic. Patient: 29-year old male basketball player. Case description: The patient presented with 2-months history of right k...
journal_title:Regenerative medicine
pub_type: 杂志文章
doi:10.2217/rme-2020-0021
更新日期:2020-06-01 00:00:00
abstract::Aim: The most common risk associated with intradiscal injection of platelet-rich plasma (PRP) is discitis with Cutibacterium acnes. It is hypothesized that antimicrobial activity of PRP can be enhanced through inclusion of leukocytes or antibiotics in the injectate. Materials & methods: Multiple PRP preparations of va...
journal_title:Regenerative medicine
pub_type: 杂志文章
doi:10.2217/rme-2019-0098
更新日期:2019-10-01 00:00:00
abstract::Many features of adipose stem/progenitor cells, including their physiological functions and localization, have been clarified in the past decade. Adipose tissue turns over very slowly, with perivascular progenitor cells differentiating into new adipocytes to replace dead adipocytes. A number of clinical trials using f...
journal_title:Regenerative medicine
pub_type: 杂志文章,评审
doi:10.2217/rme.11.62
更新日期:2011-11-01 00:00:00
abstract::Research regarding the use of cord blood (CB) has focused on antigen match and the number of stem cells, with policies and networks related to its use being under researched. This article is based on fieldwork in China from 2013 to 2015 and examines ways that the studied CB bank enhances CB usage in China. This articl...
journal_title:Regenerative medicine
pub_type: 杂志文章
doi:10.2217/rme-2017-0050
更新日期:2017-09-01 00:00:00
abstract::Cautious approaches in the clinic are currently proposed, supported by relevant in vitro, in vivo and published data. Key to developing our understanding of the risks of immune rejection of stem cell based therapies will be the inclusion of immunological endpoints in clinical trials and the sharing of data. There is l...
journal_title:Regenerative medicine
pub_type: 社论
doi:10.2217/rme.14.50
更新日期:2015-01-01 00:00:00
abstract::Since the first pioneering scientists explored the potential of using human cells for therapeutic purposes the branch of regenerative medicine has evolved to become a mature industry. The focus has switched from 'what can be done' to 'what can be commercialized'. Timely health economic evaluation supports successful m...
journal_title:Regenerative medicine
pub_type: 杂志文章
doi:10.2217/rme.13.69
更新日期:2013-11-01 00:00:00
abstract::Regenerative engineering has been defined as the convergence of Advanced Materials Sciences, Stem Cell Sciences, Physics, Developmental Biology and Clinical Translation for the regeneration of complex tissues and organ systems. Anterior cruciate ligament (ACL) reconstruction necessitates the regeneration of bone, liga...
journal_title:Regenerative medicine
pub_type: 杂志文章,评审
doi:10.2217/rme-2016-0125
更新日期:2016-12-01 00:00:00
abstract::Human embryonic stem cells offer a renewable source of a wide range of cell types for use in research and cell-based therapies. Characterizing these cells provides important information about their current state and affords relevant details for subsequent downstream manipulation. Prior to considering therapeutic appli...
journal_title:Regenerative medicine
pub_type: 杂志文章
doi:10.2217/17460751.2.2.179
更新日期:2007-03-01 00:00:00
abstract::Spinal cord injury (SCI) is a devastating traumatic injury resulting in paralysis or sensory deficits due to tissue damage and the poor ability of axons to regenerate across the lesion. Despite extensive research, there is still no effective treatment that would restore lost function after SCI. A possible therapeutic ...
journal_title:Regenerative medicine
pub_type: 杂志文章,评审
doi:10.2217/rme.11.121
更新日期:2012-03-01 00:00:00
abstract:AIMS:To investigate neural stem cell (NSC) interactions with striatal tissue following engraftment and the effects of growth factors. MATERIALS & METHODS:Organotypic striatal slice cultures established from neonatal rats were used as an ex vivo model system. Survival, integration and differentiation of grafted NSCs fr...
journal_title:Regenerative medicine
pub_type: 杂志文章
doi:10.2217/rme.10.80
更新日期:2010-11-01 00:00:00
abstract:OBJECTIVES:Mechanisms underpinning Gram-negative bacterial vaginosis-induced birth anomalies are obscure. Ethical issues limit such studies on peri-implantation-stage human embryos. Here we have used embryoid bodies (EBs) as an in vitro model to examine the effect of Gram-negative bacterial endotoxins/lipopolysaccharid...
journal_title:Regenerative medicine
pub_type: 杂志文章
doi:10.2217/17460751.3.1.23
更新日期:2008-01-01 00:00:00
abstract::Aim: To determine the potential of mesenchymal stem cells (MSC) for corneal epithelial regeneration in vitro. Materials & methods: Bone marrow MSC (BM-MSC) and adipose tissue MSC were analyzed for corneal epithelial and mesenchymal markers, using limbal stem cells and corneal cells as controls. MSC with better potenti...
journal_title:Regenerative medicine
pub_type: 杂志文章
doi:10.2217/rme-2019-0067
更新日期:2020-03-01 00:00:00
abstract::Induced pluripotent stem (iPS) cells offer tremendous opportunity for the creation of autologous cellular therapies, in which gene correction or the avoidance of immune response issues are desirable. In addition, iPS cells avoid the ethical concerns raised by the sourcing of human embryonic stem cells (hESCs) from emb...
journal_title:Regenerative medicine
pub_type: 杂志文章
doi:10.2217/rme.10.55
更新日期:2010-07-01 00:00:00
abstract::Use of clinical-grade human induced pluripotent stem cell (iPSC) lines as a starting material for the generation of cellular therapeutics requires demonstration of comparability of lines derived from different individuals and in different facilities. This requires agreement on the critical quality attributes of such l...
journal_title:Regenerative medicine
pub_type: 杂志文章
doi:10.2217/rme-2018-0095
更新日期:2018-10-01 00:00:00
abstract::Hematopoietic stem cells (HSCs) have been shown recently to hold much promise in curing autoimmune diseases. Newly diagnosed Type 1 diabetes individuals have been successfully reverted to normoglycemia by administration of autologous HSCs in association with a nonmyeloablative regimen (antithymocyte globulin + cycloph...
journal_title:Regenerative medicine
pub_type: 杂志文章,评审
doi:10.2217/rme-2015-0057
更新日期:2016-06-01 00:00:00
abstract::Aim: We aim to show that the use of nondigested micro-fragmented adipose tissue (MFat™, Lipogems®) is a viable alternative for treatment of joint pain and inflammation associated dysfunction in shoulder osteoarthritis (OA). Materials & methods: A total of 25 subjects with OA received an injection of MFat™ and were fol...
journal_title:Regenerative medicine
pub_type: 杂志文章
doi:10.2217/rme-2020-0069
更新日期:2020-10-01 00:00:00
abstract::Parkinson's disease and Alzheimer's disease are the most common neurodegenerative diseases in the elderly population. Given that age is the most important risk factor in these diseases, the number of patients is expected to rise dramatically in the coming years. Therefore, an effective therapy for these diseases is hi...
journal_title:Regenerative medicine
pub_type: 杂志文章,评审
doi:10.2217/17460751.2.4.425
更新日期:2007-07-01 00:00:00
abstract::In both Type 1 and 2 diabetes, insufficient numbers of insulin-producing beta-cells are a major cause of defective control of blood glucose and its complications. Restoration of damaged beta-cells by endocrine pancreas regeneration would be an ideal therapeutic option. The possibility of generating insulin-secreting c...
journal_title:Regenerative medicine
pub_type: 杂志文章,评审
doi:10.2217/17460751.3.3.377
更新日期:2008-05-01 00:00:00
abstract::Probably the most serious problem facing the field of regenerative medicine today is the challenge of effective translation and development of viable stem cell-based therapies. Particular concerns have been raised over the growing market in unproven cell therapies. In this article, I explore recent developments in the...
journal_title:Regenerative medicine
pub_type: 杂志文章
doi:10.2217/rme-2017-0065
更新日期:2017-10-01 00:00:00
abstract::Medicinal signaling cell (MSC)-based products represent emerging treatments in various therapeutic areas including cardiometabolic, inflammation, autoimmunity, orthopedics, wound healing and oncology. Exploring innovation beyond minimally manipulated plastic-adherent ex vivo expanded allogeneic MSCs enables product de...
journal_title:Regenerative medicine
pub_type: 杂志文章
doi:10.2217/rme-2017-0093
更新日期:2017-12-01 00:00:00
abstract::The olfactory mucosa contains cells that enable it to generate new neurons and other supporting cells throughout life, allowing it to replace cells of the mucosa that have been damaged by exposure to various insults. In this article, we discuss the different types of stem cell found within the olfactory mucosa and the...
journal_title:Regenerative medicine
pub_type: 杂志文章,评审
doi:10.2217/rme-2018-0009
更新日期:2018-07-01 00:00:00
abstract::Aim: A limiting factor in advancement of bone marrow based cell therapies is the lack of characterization of cell products delivered to patients. Methods: Using an automated hematology analyzer that can be implemented in clinical setting, the composition of bone marrow aspirates (n = 17 patients) and bone marrow conce...
journal_title:Regenerative medicine
pub_type: 临床试验,杂志文章
doi:10.2217/rme-2018-0173
更新日期:2019-07-01 00:00:00