Improved methodologies for the study of adipose biology: insights gained and opportunities ahead.

abstract：:Lipoprotein lipase has been found to efficiently mediate binding of lipoproteins to cell surfaces and to the low density lipoprotein (LDL) receptor-related protein (LRP) under cell culture conditions (Beisiegel et al. 1991. Proc. Natl. Acad. Sci. USA. 88: 8242-8346). This supports the previously proposed idea that the...

journal_title：Journal of lipid research

authors： Skottova N,Savonen R,Lookene A,Hultin M,Olivecrona G

更新日期：1995-06-01 00:00:00

abstract：:A photocolorimeter for rapid automatic titration of free fatty acids is described. The solvents, absolute ethanol and hexane, form a single-phase titration mixture containing Nile blue indicator. The titrant, NaOH in 60% ethanol, is delivered by a motor-driven microsyringe; as alkali is added and the titration mixture...

journal_title：Journal of lipid research

authors： Noble RP

更新日期：1966-11-01 00:00:00

abstract：:The dynamics of human apoE metabolism were explored by examining the effects of alimentary lipemia and postheparin lipolysis on the plasma level and lipoprotein distribution of apoE. In the studies of alimentary lipemia, fasting and postprandial plasma samples were obtained from five normal adult males, each of whom d...

journal_title：Journal of lipid research

authors： Blum CB

更新日期：1982-12-01 00:00:00

abstract：:In vitro, -polylysine (EPL) strongly inhibited the hydrolysis of trioleoylglycerol emulsified with phosphatidylcholine (PC) and taurocholate by either pancreatic lipase or carboxylester lipase. The EPL concentration required for 50% inhibition of pancreatic lipase, 0.12 microM, was eight times lower than the concentra...

journal_title：Journal of lipid research

authors： Tsujita T,Takaichi H,Takaku T,Aoyama S,Hiraki J

更新日期：2006-08-01 00:00:00

abstract：:Acyl-CoA-dependent O-acyltransferases catalyze reactions in which fatty acyl-CoAs are joined to acyl acceptors containing free hydroxyl groups to produce neutral lipids. In this report, we characterize a human multifunctional O-acyltransferase (designated MFAT) that belongs to the acyl-CoA:diacylglycerol acyltransfera...

journal_title：Journal of lipid research

authors： Yen CL,Brown CH 4th,Monetti M,Farese RV Jr

更新日期：2005-11-01 00:00:00

abstract：:Circulating apolipoprotein-defined lipoprotein subclasses (ADLS) and apolipoproteins predict vascular events in the general and type 2 diabetes populations, but data in T1D are limited. We examined associations of ADLS, serum apolipoproteins, and conventional lipids with carotid intima-media thickness (IMT) measured c...

journal_title：Journal of lipid research

authors： Basu A,Jenkins AJ,Stoner JA,Zhang Y,Klein RL,Lopes-Virella MF,Garvey WT,Schade DS,Wood J,Alaupovic P,Lyons TJ,Diabetes Control and Complications Trial\/Epidemiology of Diabetes Interventions and Complications Research Group.

更新日期：2018-05-01 00:00:00

abstract：:A reliable method for purifying envelope-stripped nuclei from immortalized murine embryonic fibroblasts (iMEFs) was established. Quantitative profiling of the glycerophospholipids (GPLs) in envelope-free iMEF nuclei yields several conclusions. First, we find the endonuclear glycerophospholipidome differs from that of ...

journal_title：Journal of lipid research

authors： Tribble EK,Ivanova PT,Grabon A,Alb JG Jr,Faenza I,Cocco L,Brown HA,Bankaitis VA

更新日期：2016-08-01 00:00:00

abstract：:Gluco- and galactocerebrosides can be separated by thin-layer chromatography on Silica Gel G prepared with sodium borate solution instead of water. The most successful developing system was chloroform-methanol-water-15 M NH(4)OH 280:70:6:1. ...

journal_title：Journal of lipid research

authors： Kean EL

更新日期：1966-05-01 00:00:00

abstract：:Ac-hE18A-NH2 is a dual-domain apoE mimetic peptide that possesses the putative receptor binding domain from apoE (LRKLRKRLLR, denoted hE; residues 141-150) covalently attached to lipid-associating peptide 18A. Like apoE, Ac-hE18A-NH2 reduces plasma cholesterol in animal models and exhibits anti-inflammatory properties...

journal_title：Journal of lipid research

authors： Anantharamaiah GM,Garber DW,Goldberg D,Morrel E,Datta G,Palgunachari MN,Register TC,Appt SE,White CR

更新日期：2018-11-01 00:00:00

abstract：:Studies on the effects of longitudinal lipid trajectories on end-stage renal disease (ESRD) development and deaths among patients with chronic kidney disease (CKD) are limited. We conducted a registry-based prospective study using data from a 13-year multidisciplinary pre-ESRD care program. The final study population ...

journal_title：Journal of lipid research

authors： Tsai CW,Huang HC,Chiang HY,Chung CW,Chang SN,Chu PL,Kuo CC

更新日期：2019-03-01 00:00:00

abstract：:The purpose of the present study was to examine the regulation of levels of apolipoprotein B (apoB) mRNA and its protein by cytokines in HepG2 cells. A dose-dependent increase in apoB mRNA levels was observed in the presence of either interleukin-1beta (IL-1beta) or IL-6 alone. This increase occurred as early as 1 h a...

journal_title：Journal of lipid research

authors： Yokoyama K,Ishibashi T,Yi-qiang L,Nagayoshi A,Teramoto T,Maruyama Y

更新日期：1998-01-01 00:00:00

abstract：:Palmitoleic acid is a minor monounsaturated fatty acid in the human diet and in blood plasma. Because macadamia oil is at least one potentially large source of palmitoleic acid, we tested its effect on plasma lipid levels against two other dietary fatty acids, oleic acid and palmitic acid. The dietary adjustments, thr...

journal_title：Journal of lipid research

authors： Nestel P,Clifton P,Noakes M

更新日期：1994-04-01 00:00:00

abstract：:The increased generation of reactive oxygen species that occurs in the condition of obesity may be responsible for oxidative injury to erythrocyte membranes, which could lead to a decrease in tissue oxygenation. Therefore, we have looked into the effects of obesity on both indexes of oxidative damage and physical-chem...

journal_title：Journal of lipid research

authors： Cazzola R,Rondanelli M,Russo-Volpe S,Ferrari E,Cestaro B

更新日期：2004-10-01 00:00:00

abstract：:The following data have been obtained from comparative studies on the metabolism of cholesterol, cholestanol, and beta-sitosterol by L-cell mouse fibroblasts. (1) When the sterols are added to the growth medium under similar conditions, cellular incorporation of cholesterol > cholestanol > beta-sitosterol; (2) only li...

journal_title：Journal of lipid research

authors： Rothblat GH,Burns CH

更新日期：1971-11-01 00:00:00

abstract：:The regulation of hepatic and intestinal 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) reductase and acyl-CoA; cholesterol acyltransferase (ACAT) activities by dietary fish oil was examined in the rabbit. Rabbits were fed 10% menhaden oil or menhaden oil plus 1% cholesterol for 14 days. They were compared with animals fed ...

journal_title：Journal of lipid research

authors： Field FJ,Albright EJ,Mathur SN

更新日期：1987-01-01 00:00:00

abstract：:An amino group of phosphatidylethanolamine (PE) is considered as a target for nonenzymatic glycation, and the potential involvement of lipid glycation in the pathogenesis of diabetic complications has generated interest. However, unlike an early glycation product of PE (Amadori-PE), the occurrence and roles of advance...

journal_title：Journal of lipid research

authors： Shoji N,Nakagawa K,Asai A,Fujita I,Hashiura A,Nakajima Y,Oikawa S,Miyazawa T

更新日期：2010-08-01 00:00:00

abstract：:Previously, our laboratory demonstrated that ceramide-1-phosphate (C1P) specifically activated group IVA cytosolic phospholipase A(2) (cPLA(2)alpha) in vitro. In this study, we investigated the chain length specificity of this interaction. C1P with an acyl-chain of >or=6 carbons efficiently activated cPLA(2)alpha in v...

journal_title：Journal of lipid research

authors： Wijesinghe DS,Subramanian P,Lamour NF,Gentile LB,Granado MH,Bielawska A,Szulc Z,Gomez-Munoz A,Chalfant CE

更新日期：2009-10-01 00:00:00

abstract：:In hepatocytes, vitamin E is secreted via the efflux pathway and is believed to associate with apolipoprotein B (apoB)-lipoproteins extracellularly. The molecular mechanisms involved in the uptake, intracellular trafficking, and secretion of dietary vitamin E by the intestinal cells are unknown. We observed that low c...

journal_title：Journal of lipid research

authors： Anwar K,Kayden HJ,Hussain MM

更新日期：2006-06-01 00:00:00

abstract：:Mammalian spermatogenesis is a complex developmental program in which a diploid progenitor germ cell transforms into highly specialized spermatozoa. One intriguing aspect of sperm production is the dynamic change in membrane lipid composition that occurs throughout spermatogenesis. Cholesterol content, as well as its ...

journal_title：Journal of lipid research

authors： Keber R,Rozman D,Horvat S

更新日期：2013-01-01 00:00:00

abstract：:Series of benzoolysis experiments with 1,2-dipalmitoyl glycerophosphocholine (GPC), 1-palmitoyl-2-lyso GPC, and 1-O-hexadecenyl-2-oleoyl GPC, as well as a few experiments with 1-oleoyl-2-acetyl GPC and 1-O-hexadecyl-2-acetyl GPC are reported. The results are used to present a consistent picture of the various dephosph...

journal_title：Journal of lipid research

authors： Gelsema WJ,van den Bosch H

更新日期：1997-08-01 00:00:00

abstract：:Conventional ideas concerning the unidirectional movement of triacylglycerol from intestinal lumen to lymph with sn-2-monoacylglycerol being the major glyceride-glycerol precursor were challenged by our finding that steady state specific activities of radiolabeled triacylglycerol (glyceryl moiety) in the intestinal mu...

journal_title：Journal of lipid research

authors： Mansbach CM 2nd,Parthasarathy S

更新日期：1982-09-01 00:00:00

abstract：:The differentiation of macrophages into lipid-filled foam cells is a hallmark of the lung granuloma that forms in patients with active tuberculosis (TB). Mycolic acids (MAs), the abundant lipid virulence factors in the cell wall of Mycobacterium tuberculosis (Mtb), can induce this foam phenotype possibly as a way to p...

journal_title：Journal of lipid research

authors： Vermeulen I,Baird M,Al-Dulayymi J,Smet M,Verschoor J,Grooten J

更新日期：2017-04-01 00:00:00

abstract：:The fatty acid composition of human skin fibroblasts grown in 10% dialyzed fetal calf serum can be modified considerably by adding supplemental fatty acids to the culture medium. The degree of modification was dependent on the concentration of added fatty acid over the range tested, 2.5 X 10(-5) to 1 X 10(-4) M. At th...

journal_title：Journal of lipid research

authors： Spector AA,Kiser RE,Denning GM,Koh SW,DeBault LE

更新日期：1979-05-01 00:00:00

abstract：:Vitamin A-transporting protein in chicken plasma was purified by column chromatography on DEAE-Sephadex and Sephadex G-100; the protein formed a complex of retinol-binding protein (RBP) with prealbumin (PA). The molecular weight of the 1:1 molar complex was estimated to be 76,000 by gel filtration, and the sedimentati...

journal_title：Journal of lipid research

authors： Abe T,Muto Y,Hosoya N

更新日期：1975-05-01 00:00:00

abstract：:Circulating concentrations of mevalonic acid (MVA) change in parallel with, and may be used as a marker of cholesterol biosynthesis. Plasma MVA levels have been quantified using a sensitive and specific capillary gas chromatography-electron capture mass spectrometric assay. The detection limit for MVA in plasma is 100...

journal_title：Journal of lipid research

authors： Scoppola A,Maher VM,Thompson GR,Rendell NB,Taylor GW

更新日期：1991-06-01 00:00:00

abstract：:The isolated perfused rat liver was used to study the 300-800 A electron-opaque bodies which had previously been described in the liver cell Golgi apparatus, smooth endoplasmic reticulum, and space of Disse. When the perfusion medium was enriched with linoleate, the number and electron opacity of these particles incre...

journal_title：Journal of lipid research

authors： Jones AL,Ruderman NB,Herrera MG

更新日期：1967-09-01 00:00:00

abstract：:Potentially atherogenic triglyceride-rich lipoprotein (TRL) remnants can be isolated and quantitated as remnant-like particles (RLP), using an immunoaffinity gel containing specific anti-human apolipoprotein A-I (apoA-I) and apoB-100 monoclonal antibodies. The aim of the present study was to determine the relationship...

journal_title：Journal of lipid research

authors： Marcoux C,Hopkins PN,Wang T,Leary ET,Nakajima K,Davignon J,Cohn JS

更新日期：2000-09-01 00:00:00

abstract：:Here, we present an improved method for sensitive profiling of lipids in a single high-performance liquid chromatography-electrospray ionization-quadrupole time of flight mass spectrometry experiment. The approach consists of i) sensitive isocratic elution, which takes advantage of C18 column material that is resistan...

journal_title：Journal of lipid research

authors： Shui G,Bendt AK,Pethe K,Dick T,Wenk MR

更新日期：2007-09-01 00:00:00

abstract：:In the present study, we have examined the structure of human low density lipoprotein (LDL) using cryo-electron microscopy. Human LDL particles were analyzed in a vitrified frozen-hydrated condition, without chemical fixation or any form of staining. Hence, the lipoproteins were visualized close to their native state....

journal_title：Journal of lipid research

authors： Van Antwerpen R,Gilkey JC

更新日期：1994-12-01 00:00:00

abstract：:Experimental approaches involving the perfusion of tissues and organs offer the advantage of improved physiological relevance over the use of isolated tissues or cells while at the same time being much more controlled and tissue-specific than studies in vivo. Nevertheless, there have been few metabolic studies perform...

journal_title：Journal of lipid research

authors： Cònsol G,Moles A,Ricart-Jané D,Llobera M

更新日期：2005-08-01 00:00:00