Sterols in spermatogenesis and sperm maturation.

abstract：:Nine even-numbered saturated (C4 to C20) and two unsaturated (C18:1, C18:2) cholesterol alkyl ethers were studied by differential scanning calorimetry, polarizing microscopy, and X-ray powder diffraction. Seven of the nine saturated ethers examined melt into stable liquid crystal mesophases. Mesophase transition tempe...

journal_title：Journal of lipid research

authors： Deckelbaum RJ,Halperin G,Atkinson D

更新日期：1983-05-01 00:00:00

abstract：:The acylation of glycerol-3-phosphate by acyl-CoA is regarded as the first committed step for the synthesis of the lipoidal moiety in glycerolipids. The direct acylation of glycerol in mammalian tissues has not been demonstrated. In this study, lipid biosynthesis in myoblasts and hepatocytes was reassessed by conducti...

journal_title：Journal of lipid research

authors： Lee DP,Deonarine AS,Kienetz M,Zhu Q,Skrzypczak M,Chan M,Choy PC

更新日期：2001-12-01 00:00:00

abstract：:The major high density lipoprotein (HDL) apolipoprotein, apoA-I, was knocked out by gene targeting in ES cells to provide a model for the study of HDL metabolism and its relationship to plasma and tissue cholesterol metabolism. HDL and non-HDL cholesterol (HDL-C) were reduced in apoA-I-deficient mice. Feeding a high f...

journal_title：Journal of lipid research

authors： Plump AS,Azrolan N,Odaka H,Wu L,Jiang X,Tall A,Eisenberg S,Breslow JL

更新日期：1997-05-01 00:00:00

abstract：:We analysed the sensitivity to beta-adrenoceptor agonists in epididymal adipose cells from rats submitted to a stress protocol previously reported to induce alterations in sensitivity to catecholamines in cardiac tissue from rats. Food intake and body weight were lower, whereas adipocytes basal lipolysis was higher (c...

journal_title：Journal of lipid research

authors： Farias-Silva E,Grassi-Kassisse DM,Wolf-Nunes V,Spadari-Bratfisch RC

更新日期：1999-09-01 00:00:00

abstract：:Our previous studies have demonstrated the activity of oncostatin M (OM) in stimulating the transcription of the human LDL receptor (LDLR) gene in HepG2 cells through a sterol-independent regulatory mechanism. The current studies were designed to determine whether this in vitro property of OM could be recapitulated in...

journal_title：Journal of lipid research

authors： Kong W,Abidi P,Kraemer FB,Jiang JD,Liu J

更新日期：2005-06-01 00:00:00

abstract：:Triglyceride (TG) concentration is used as a marker of cardiometabolic risk. However, diurnal and possibly weekday variation exists in TG concentrations. The objective of this work was to investigate weekday variation in TG concentrations among 1.8 million blood samples drawn between 2008 and 2015 from patients in the...

journal_title：Journal of lipid research

authors： Jaskolowski J,Ritz C,Sjödin A,Astrup A,Szecsi PB,Stender S,Hjorth MF

更新日期：2017-06-01 00:00:00

abstract：:Niemann-Pick disease, type C (NP-C), often associated with Niemann-Pick disease, type C1 (NPC1) mutations, is a cholesterol-storage disorder characterized by cellular lipid accumulation, neurodegeneration, and reduced steroid production. To study NPC1 function in vivo, we cloned zebrafish npc1 and analyzed its gene ex...

journal_title：Journal of lipid research

authors： Schwend T,Loucks EJ,Snyder D,Ahlgren SC

更新日期：2011-07-01 00:00:00

abstract：:In breast cancer, 17β-estradiol (E2) plays critical roles mainly by binding to its canonical receptor, estrogen receptor (ER) α66, and eliciting genomic effects. E2 also triggers rapid, nongenomic responses. E2 activates sphingosine kinase 1 (SphK1), increasing sphingosine-1-phosphate (S1P) that binds to its receptors...

journal_title：Journal of lipid research

authors： Maczis MA,Maceyka M,Waters MR,Newton J,Singh M,Rigsby MF,Turner TH,Alzubi MA,Harrell JC,Milstien S,Spiegel S

更新日期：2018-12-01 00:00:00

abstract：:We have previously shown that expression of a human apoE cDNA in J774 macrophages enhances cholesterol efflux to HDL3. We have also shown that endogenous apoE expression produces a cell surface pool of apoE associated with proteoglycans. In this series of experiments, we first demonstrate the presence of a cell surfac...

journal_title：Journal of lipid research

authors： Lin CY,Lucas M,Mazzone T

更新日期：1998-02-01 00:00:00

abstract：:X-linked adrenoleukodystrophy (X-ALD) is a peroxisomal disorder caused by impaired degradation of very long-chain fatty acids (VLCFAs) due to mutations in the ABCD1 gene responsible for VLCFA transport into peroxisomes. Lorenzo's oil, a 4:1 mixture of glyceryl trioleate and glyceryl trierucate, has been used to reduce...

journal_title：Journal of lipid research

authors： Sassa T,Wakashima T,Ohno Y,Kihara A

更新日期：2014-03-01 00:00:00

abstract：:LCAT plays a key role in the maturation of HDL, as evidenced by low HDL-cholesterol levels in carriers of deleterious mutations in LCAT. However, the role of LCAT in atherosclerosis is unclear. We set out to study this in a prospective study. Plasma LCAT levels, which strongly correlate with LCAT activity, were measur...

journal_title：Journal of lipid research

authors： Holleboom AG,Kuivenhoven JA,Vergeer M,Hovingh GK,van Miert JN,Wareham NJ,Kastelein JJ,Khaw KT,Boekholdt SM

更新日期：2010-02-01 00:00:00

abstract：:Endothelial lipase (EL) has been shown to be a critical determinant for high density lipoprotein cholesterol levels in vivo; therefore, assays that measure EL activity have become important for the discovery of small molecule inhibitors that specifically target EL. Here, we describe fluorescent Bodipy-labeled substrat...

journal_title：Journal of lipid research

authors： Mitnaul LJ,Tian J,Burton C,Lam MH,Zhu Y,Olson SH,Schneeweis JE,Zuck P,Pandit S,Anderson M,Maletic MM,Waddell ST,Wright SD,Sparrow CP,Lund EG

更新日期：2007-02-01 00:00:00

abstract：:Choline glycerophospholipids and ethanolamine glycerophospholipids of ruminant skeletal muscle contain approximately 40% and 65% plasmalogen, respectively. In the 1,2-diacyl-sn-glycero-3-phosphocholine (diacyl CPG), 16:0-18:2(n-6) and 16:0-18:1(n-9) accounted for about 50% of the total molecular species; in the 2-acyl...

journal_title：Journal of lipid research

authors： Scott TW,Ashes JR,Fleck E,Gulati SK

更新日期：1993-05-01 00:00:00

abstract：:The implication of cholesteryl ester transfer protein (CETP) in the high density lipoprotein (HDL) conversion process was studied by incubating HDL3 with a purified CETP preparation for 24 h at 37 degrees C. At a physiological plasma level, CETP induced a decrease of the HDL3 fraction (8.6 nm diameter) and the appeara...

journal_title：Journal of lipid research

authors： Lagrost L,Gambert P,Dangremont V,Athias A,Lallemant C

更新日期：1990-09-01 00:00:00

abstract：:Oxidative stress promotes acute kidney injury (AKI). Higher concentrations of HDL cholesterol are associated with less AKI. To test the hypothesis that HDL antioxidant activity is associated with AKI after cardiac surgery, we quantified HDL particle size and number, paraoxonase-1 activity, and isofuran concentrations ...

journal_title：Journal of lipid research

authors： Smith LE,Smith DK,Yancey PG,Kon V,Remaley AT,Billings FT 4th,Linton MF

更新日期：2021-01-13 00:00:00

abstract：:Hepatocytes express on their surfaces more than one class of receptors capable of mediating the internalization of lipoproteins. However, relatively little is known about the binding characteristics of hepatic receptors for various lipoproteins, about the regulation of the receptors, and about the consequences for int...

journal_title：Journal of lipid research

authors： Tamai T,Patsch W,Lock D,Schonfeld G

更新日期：1983-12-01 00:00:00

abstract：:Rat spermatogenic cells contain sphingomyelins (SMs) and ceramides (Cers) with very long-chain PUFAs (VLCPUFAs) in nonhydroxylated (n-V) and 2-hydroxylated (h-V) forms. How these atypical species distribute among membrane fractions during differentiation was investigated here using a detergent-free procedure to isolat...

journal_title：Journal of lipid research

authors： Santiago Valtierra FX,Mateos MV,Aveldaño MI,Oresti GM

更新日期：2017-03-01 00:00:00

abstract：:A simple procedure is described for preparing lyso-GM1, a GM1 derivative that lacks the fatty acid moiety, starting from GM1 ganglioside using a one-pot reaction. Ganglioside deacylation was carried out in KOH/propan-1-ol in the absence of oxygen. The yield of lyso-GM1 under optimal conditions (6 h, 90 degrees C, 0.2 ...

journal_title：Journal of lipid research

authors： Sonnino S,Acquotti D,Kirschner G,Uguaglianza A,Zecca L,Rubino F,Tettamanti G

更新日期：1992-08-01 00:00:00

abstract：:At 123 K, crystals of cholesteryl cis-9-hexadecenoate (cholesteryl palmitoleate, C45H74O2) are monoclinic, space group P2(1) with cell dimensions a = 12.917(7), b = 8.910(5), c = 34.04(1) A, beta = 94.95(7) degrees [lambda(CuK alpha) = 1.5424 A] having two independent molecules (A and B) per unit cell. The crystal str...

journal_title：Journal of lipid research

authors： Craven BM,Sawzik P

更新日期：1984-08-01 00:00:00

abstract：:Albumin-bound long-chain fatty acid methyl esters (ME) were taken up and utilized by Ehrlich ascites tumor cells and slices of rat heart, liver, and kidney. Much more ME than albumin was taken up by the tumor cells, indicating that ME dissociated from the carrier protein during their uptake. 70-80% of the radioactivit...

journal_title：Journal of lipid research

authors： Kuhl WE,Spector AA

更新日期：1970-09-01 00:00:00

abstract：:The conversion of alpha-linolenate (18:3n-3) to docosahexaenoate (22:6n-3) in the presence of low and high dietary levels of linoleate (18:2n-6) is reported in young rats using [U-13C]-alpha-linolenic acid (18:2n-3*) and high precision gas chromatography-combustion isotope ratio mass spectrometry (GCC-IRMS). After con...

journal_title：Journal of lipid research

authors： Sheaff RC,Su HM,Keswick LA,Brenna JT

更新日期：1995-05-01 00:00:00

abstract：:There is growing evidence of the capacity of vitamin A to regulate the expression of the genetic region that encodes apolipoproteins (apo) A-I, C-III, and A-IV. This region in turn has been proposed to modulate the expression of hyperlipidemia in the commonest genetic form of dyslipidemia, familial combined hyperlipid...

journal_title：Journal of lipid research

authors： Ribalta J,Girona J,Vallvé JC,La Ville AE,Heras M,Masana L

更新日期：1999-03-01 00:00:00

abstract：:Lysophosphatidic acid (LPA) is a receptor-active lipid mediator with a broad range of biological effects. Ovarian cancer cells synthesize LPA, which promotes their motility, growth, and survival. We show that a murine homolog of a human protein previously reported to hydrolyze LPA is a highly selective detergent-stimu...

journal_title：Journal of lipid research

authors： Luquain C,Singh A,Wang L,Natarajan V,Morris AJ

更新日期：2003-10-01 00:00:00

abstract：:Thus far, liver, intestine, heart, and placenta have been shown to secrete apolipoprotein (apo)B-containing lipoproteins. In the present study, we first investigated lipoproteins in human follicular fluid (FF), surrounding developing oocytes within the ovary, as well as in corresponding plasma samples (n = 12). HDL ch...

journal_title：Journal of lipid research

authors： Gautier T,Becker S,Drouineaud V,Ménétrier F,Sagot P,Nofer JR,von Otte S,Lagrost L,Masson D,Tietge UJ

更新日期：2010-08-01 00:00:00

abstract：:The low density lipoprotein receptor (LDLR) regulates the plasma cholesterol level by mediating endocytosis of LDL. We established stable hamster cell lines expressing two LDLRs with distinct functional defects, i.e., endocytosis and ligand binding. In the cell line expressing only I189D h/r (human-rat chimeric) LDLR,...

journal_title：Journal of lipid research

authors： Yoshida H,Yokode M,Yamamoto A,Masaki R,Murayama T,Horiuchi H,Kita T

更新日期：1999-05-01 00:00:00

abstract：:Serum amyloid P (SAP) is a common component of human amyloid deposits and has been identified in atherosclerotic lesions. We investigated the extent of the colocalization of SAP with apolipoprotein A-I (apoA-I), apoB, apoC-II, and apoE in human coronary arteries and explored potential roles for SAP in these regions, s...

journal_title：Journal of lipid research

authors： Stewart CR,Haw A 3rd,Lopez R,McDonald TO,Callaghan JM,McConville MJ,Moore KJ,Howlett GJ,O'Brien KD

更新日期：2007-10-01 00:00:00

abstract：:Biliary secretion of bile acid glucuronides was studied in control rats and in rats with a congenital defect in hepatobiliary transport of organic anions (GY rats). In control animals, hepatobiliary transport of [3H]lithocholic acid 3-O-glucuronide and [3H]cholic acid 3-O-glucuronide was efficient (greater than 95% in...

journal_title：Journal of lipid research

authors： Kuipers F,Radominska A,Zimniak P,Little JM,Havinga R,Vonk RJ,Lester R

更新日期：1989-12-01 00:00:00

abstract：:Ecdysteroids are potent developmental regulators that control molting, reproduction, and stress response in arthropods. In developing larvae, picogram quantities of individual ecdysteroids and their conjugated forms are present along with milligrams of structural and energy storage lipids. To enhance the specificity a...

journal_title：Journal of lipid research

authors： Lavrynenko O,Nedielkov R,Möller HM,Shevchenko A

更新日期：2013-08-01 00:00:00

abstract：:Purified liver plasma membranes were prepared from rats fed a high-fat, carbohydrate-free diet or a high-glucose, fat-free diet. Membranes from rats fed the high-fat diet bound significantly less 125I-labeled concanavalin A (Con A) than did those from rats fed the fat-free diets. The magnitude of the binding differenc...

journal_title：Journal of lipid research

authors： Henriquez DS,Tepperman HM,Tepperman J

更新日期：1979-07-01 00:00:00

abstract：:Small angle X-ray diffraction was used to examine arterial smooth muscle cell (SMC) plasma membranes isolated from control and cholesterol-fed (2%) atherosclerotic rabbits. A microsomal membrane enriched with plasma membrane obtained from animals fed cholesterol for up to 13 weeks showed a progressive elevation in the...

journal_title：Journal of lipid research

authors： Tulenko TN,Chen M,Mason PE,Mason RP

更新日期：1998-05-01 00:00:00