Abstract:
:A simple procedure is described for preparing lyso-GM1, a GM1 derivative that lacks the fatty acid moiety, starting from GM1 ganglioside using a one-pot reaction. Ganglioside deacylation was carried out in KOH/propan-1-ol in the absence of oxygen. The yield of lyso-GM1 under optimal conditions (6 h, 90 degrees C, 0.2 N KOH, 1 mM GM1) was 54%. The chemical structure of lyso-GM1 was determined by 1H-NMR and FAB-MS analyses, thus proving that the acetamide groups of galactosamine and sialic acid units were not affected during the deacylation reaction.
journal_name
J Lipid Resjournal_title
Journal of lipid researchauthors
Sonnino S,Acquotti D,Kirschner G,Uguaglianza A,Zecca L,Rubino F,Tettamanti Gkeywords:
subject
Has Abstractpub_date
1992-08-01 00:00:00pages
1221-6issue
8eissn
0022-2275issn
1539-7262journal_volume
33pub_type
杂志文章abstract::The subcellular and submitochondrial localization of CTP:phosphatidate cytidylyltransferase is altered in the Morris 7777 hepatoma. Mitochondria in this poorly differentiated tumor are the principal sites of CDP-diacylglycerol synthesis, in contrast to normal rat liver where the endoplasmic reticulum is most active. T...
journal_title:Journal of lipid research
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journal_title:Journal of lipid research
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journal_title:Journal of lipid research
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.RA119000119
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M011825
更新日期:2011-02-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M600323-JLR200
更新日期:2007-05-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.D008391
更新日期:2010-11-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1973-03-01 00:00:00
abstract::Triglyceride (TG) concentration is used as a marker of cardiometabolic risk. However, diurnal and possibly weekday variation exists in TG concentrations. The objective of this work was to investigate weekday variation in TG concentrations among 1.8 million blood samples drawn between 2008 and 2015 from patients in the...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M074062
更新日期:2017-06-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.P026005
更新日期:2012-07-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章,评审
doi:10.1194/jlr.R062760
更新日期:2016-03-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1989-02-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1970-07-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章,评审
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更新日期:2015-06-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.D007146
更新日期:2010-08-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1996-01-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1996-04-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1990-09-01 00:00:00
abstract::The beta-oxidation of stearic acid and of alpha- and gamma-methyl isoprenoid-derived fatty acids (pristanic and tetramethylheptadecanoic acids, respectively) was investigated in normal skin fibroblasts and in fibroblasts from patients with inherited defects in peroxisomal biogenesis. Stearic acid beta-oxidation by nor...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1990-02-01 00:00:00
abstract::Unesterified cholesterol is an essential structural component of the plasma membrane of every cell. During evolution, this membrane came to play an additional, highly specialized role in the central nervous system (CNS) as the major architectural component of compact myelin. As a consequence, in the human the mean con...
journal_title:Journal of lipid research
pub_type: 杂志文章,评审
doi:10.1194/jlr.R400004-JLR200
更新日期:2004-08-01 00:00:00
abstract::Enzymes associated with glycerolipid biosynthesis were examined in microsomal fractions of liver and adipose tissue obtained from swine of various ages. Generally, liver glycerophosphate acyltransferase, phosphatidate phosphohydrolase, diglyceride acyltransferase, and choline phosphotransferase activities were substan...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1979-02-01 00:00:00
abstract::In isolated fat cells, the same maximal rate of glycerol production can be induced by epinephrine or ACTH, alone or in combination with each other or with glucagon. With fat cells from rats weighing 150-175 g, the maximal rate of lipolysis attained with glucagon was 75-80% of that produced by epinephrine or ACTH, and ...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1972-01-01 00:00:00
abstract::In the present study, the relative positions of 11 anti-apolipoprotein B monoclonal antibodies have been mapped onto the surface of human low density lipoproteins by electron microscopy. As the epitopes recognized by these antibodies have been previously located on the primary sequence of apoB, these data provide a ma...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1995-09-01 00:00:00
abstract::The biosynthesis of prostaglandins by isolated rat adrenocortical cells has been studied by determinations of products formed during incubations with labeled arachidonic acid and by radioimmunoassays. Analysis by thin-layer chromatographic separation of silicic acid column fractions indicated that PGE2, PGA2, (B2) and...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1979-01-01 00:00:00
abstract::Within the cholesterol biosynthesis cascade, the enzyme 2,3-oxidosqualene cyclase [EC 5.4.99.7] is of special interest due to its dual function: cyclization of 2,3-monoepoxysqualene to lanosterol and 2,3;22,23-diepoxysqualene to oxylanosterol. Further determination of the significance of this enzyme for the intracellu...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1996-01-01 00:00:00
abstract::Mitochondrial sterol 27-hydroxylase (EC 1.14.13.15) is an important enzyme, not only in the formation of bile acids from cholesterol intermediates in the liver but also in the removal of cholesterol by side chain hydroxylation in extrahepatic tissues. The enzyme has been assayed by complicated methods using radiolabel...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.D200045-JLR200
更新日期:2003-12-01 00:00:00
abstract::In Tangier disease, absence of ATP binding cassette transporter A1 (ABCA1) results in reduced plasma HDL and elevated triglyceride (TG) levels. We hypothesized that hepatocyte ABCA1 regulates VLDL TG secretion through nascent HDL production. Silencing of ABCA1 expression in oleate-stimulated rat hepatoma cells resulte...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M900083
更新日期:2010-04-01 00:00:00
abstract::The time course of the clearance from the blood and the tissue localization of [14C]L-glucosylceramide, a nonmetabolizable enantiomorph of D-glucosylceramide that accumulates in Gaucher's disease, has been determined. 14C-labeled L-glucosylceramide injected intravenously in the form of micelles or liposomes is rapidly...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1987-08-01 00:00:00
abstract::Serum paraoxonase (PON1) is a HDL-associated enzyme exhibiting potentially antiatherogenic properties. Here, we examined the common PON1-192R/Q human polymorphism. Despite numerous studies, the effect of this polymorphism on the antiatherogenic potential of PON1 is yet unresolved. Our structural model suggests that am...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M600297-JLR200
更新日期:2006-11-01 00:00:00