Comprehensive analysis of the major lipid classes in sebum by rapid resolution high-performance liquid chromatography and electrospray mass spectrometry.

abstract：:It has been demonstrated that the alkenylacyl class of ethanolamine phospholipid (PE) represents one of the major forms of eicosapentaenoic acid (EPA)-containing phospholipid in the circulating platelets isolated from human subjects consuming a fish oil concentrate. Since the alkenylacyl PE from human platelets is enr...

journal_title：Journal of lipid research

authors： Aukema HM,Holub BJ

更新日期：1989-01-01 00:00:00

abstract：:In addition to functioning as detergents that aid digestion of dietary lipids in the intestine, some bile acids have been shown to exhibit antimicrobial activity. However, detailed information on the bactericidal activities of the diverse molecular species of bile acid in humans and rodents is largely unknown. Here, w...

journal_title：Journal of lipid research

authors： Watanabe M,Fukiya S,Yokota A

更新日期：2017-06-01 00:00:00

abstract：:A rapid and accurate method is described for the assay of cholesterol ester hydrolase (CEH) activity. Aliquots of the enzyme-substrate incubation mixture are extracted into isopropanol. The free cholesterol concentration in each extract is determined enzymatically using a single aqueous reagent containing cholesterol ...

journal_title：Journal of lipid research

authors： Gallo LL,Atasoy R,Vahouny GV,Treadwell CR

更新日期：1978-09-01 00:00:00

abstract：:Gel-permeation chromatography on Sephadex LH-20, using ethanol as eluent, permits the resolution of neutral hydroxy lipids according to molecular size. The influence of molecular shape, functional groups, chain lengths, and degree of unsaturation, as well as the effect of the eluent on the elution pattern are discusse...

journal_title：Journal of lipid research

authors： Calderon M,Baumann WJ

更新日期：1970-03-01 00:00:00

abstract：:13C-NMR spectroscopy was used to examine the effect of side chain length on the ionization properties and transmembrane transport rate of 3 alpha,7 alpha-dihydroxy bile acids. When solubilized in taurocholate micelles, [23-13C]nor-chenodeoxycholic acid (nor-CDCA) had a pKa of 6.1, similar to that of CDCA (pKa 6.2), it...

journal_title：Journal of lipid research

authors： Ko J,Hamilton JA,Ton-Nu HT,Schteingart CD,Hofmann AF,Small DM

更新日期：1994-05-01 00:00:00

abstract：:Placental fatty acid transport and metabolism are important for proper growth and development of the feto-placental unit. The nuclear receptors, liver X receptors alpha and beta (LXRalpha and LXRbeta), are key regulators of lipid metabolism in many tissues, but little is known about their role in fatty acid transport ...

journal_title：Journal of lipid research

authors： Weedon-Fekjaer MS,Dalen KT,Solaas K,Staff AC,Duttaroy AK,Nebb HI

更新日期：2010-07-01 00:00:00

abstract：:Tetradecylthioacetic acid (TTA), which cannot be beta-oxidized, exerts growth-limiting properties in glioma cells. In order to investigate the importance of modulated lipid metabolism and alterations in mitochondrial properties in this cell death process, we incubated glioma cells both with TTA and the oxidizable fatt...

journal_title：Journal of lipid research

authors： Berge K,Tronstad KJ,Bohov P,Madsen L,Berge RK

更新日期：2003-01-01 00:00:00

abstract：:Plasma phospholipid transfer protein (PLTP) interacts with HDL particles and facilitates the transfer of phospholipids from triglyceride (TG)-rich lipoproteins to HDL. Overexpressing human PLTP in mice increases the susceptibility to atherosclerosis. In human plasma, high-active and low-active forms of PLTP exist. To ...

journal_title：Journal of lipid research

authors： Samyn H,Moerland M,van Gent T,van Haperen R,Metso J,Grosveld F,Jauhiainen M,van Tol A,de Crom R

更新日期：2008-12-01 00:00:00

abstract：:A rapid isocratic method for determining the total phosphatidylcholine and disaturated phosphatidylcholine levels in lung surfactant preparations by high performance liquid chromatography (HPLC) is described. The analysis was performed on a 3.9 x 300 mm mu-Porasil column with detection by refractive index. The lipids ...

journal_title：Journal of lipid research

authors： Scarim J,Ghanbari H,Taylor V,Menon G

更新日期：1989-04-01 00:00:00

abstract：:Previous studies from our laboratory have shown that oral administration of stigmastanyl-phosphocholine (Ro 16-6532) reduces plasma cholesterol levels in experimental animals on diets free of added cholesterol. In the present study, effects of Ro 16-6532 and lovastatin on lipoprotein levels and metabolism were investi...

journal_title：Journal of lipid research

authors： Himber J,Missano B,Rudling M,Hennes U,Kempen HJ

更新日期：1995-07-01 00:00:00

abstract：:Male Sprague-Dawley rats were fed diets that contained 20% by weight soybean oil or rapeseed oil (21% and 43% erucic acid) for 7 days. The rapeseed oil diets increased the cardiac triacylglycerol content 5-fold and 25-fold, respectively, above control values. Hearts were removed from the animals and perfused with modi...

journal_title：Journal of lipid research

authors： Stewart LC,Kramer JK,Sauer FD,Clarke K,Wolynetz MS

更新日期：1993-09-01 00:00:00

abstract：:In mammals, vitamin A is primarily stored as retinyl esters in hepatic stellate cells under normal dietary intake of the vitamin. Previously, extrahepatic vitamin A-storing stellate cells have only been identified in animals maintained on a vitamin A-rich diet, and it has not been known whether these cells play a role...

journal_title：Journal of lipid research

authors： Nagy NE,Holven KB,Roos N,Senoo H,Kojima N,Norum KR,Blomhoff R

更新日期：1997-04-01 00:00:00

abstract：:Supersaturation and rapid nucleation of cholesterol in bile are of key importance in the pathogenesis of cholesterol gallstones. While the effects of bile acids and phospholipids on cholesterol saturation of bile have been extensively studied, their influence on the cholesterol nucleation time has not been compared. W...

journal_title：Journal of lipid research

authors： Jüngst D,Lang T,Huber P,Lange V,Paumgartner G

更新日期：1993-09-01 00:00:00

abstract：:Effects of serum cholesterol on cholesterol content in the retina are currently unknown. It is also unclear how cholesterol levels are controlled in the retina. High-cholesterol diet and oral administrations of simvastatin were used to modulate serum cholesterol in mice. These treatments only modestly affected cholest...

journal_title：Journal of lipid research

authors： Zheng W,Mast N,Saadane A,Pikuleva IA

更新日期：2015-01-01 00:00:00

abstract：:ATP-citrate lyase (ACL) is a key lipogenic enzyme that converts citrate in the cytoplasm to acetyl-CoA, the initial precursor that yields malonyl-CoA for fatty acid biosynthesis. As cytosolic citrate is derived from the tricarboxylic acid cycle in the mitochondrion, ACL catalyzes a critical reaction linking cellular g...

journal_title：Journal of lipid research

authors： Wang Q,Li S,Jiang L,Zhou Y,Li Z,Shao M,Li W,Liu Y

更新日期：2010-09-01 00:00:00

abstract：:Ceramides are the predominant lipids in the stratum corneum (SC) and are crucial components for normal skin barrier function. Although the composition of various ceramide classes in the human SC has been reported, that in mice is still unknown, despite mice being widely used as animal models of skin barrier function. ...

journal_title：Journal of lipid research

authors： Kawana M,Miyamoto M,Ohno Y,Kihara A

更新日期：2020-06-01 00:00:00

abstract：:Lysosomal acid lipase (LAL) is essential for the hydrolysis of triglycerides (TG) and cholesteryl esters (CE) in lysosomes. A mouse model created by gene targeting produces no LAL mRNA, protein, or enzyme activity. The lal-/- mice appear normal at birth, survive into adulthood, and are fertile. Massive storage of TG a...

journal_title：Journal of lipid research

authors： Du H,Heur M,Duanmu M,Grabowski GA,Hui DY,Witte DP,Mishra J

更新日期：2001-04-01 00:00:00

abstract：:SREBP cleavage-activating protein (Scap) is an endoplasmic reticulum membrane protein required for cleavage and activation of sterol regulatory element-binding proteins (SREBPs), which activate the transcription of genes in sterol and fatty acid biosynthesis. Liver-specific loss of Scap is well tolerated; hepatic synt...

journal_title：Journal of lipid research

authors： McFarlane MR,Cantoria MJ,Linden AG,January BA,Liang G,Engelking LJ

更新日期：2015-08-01 00:00:00

abstract：:Lysophospholipid (LP) research has experienced a period of renaissance with the discovery of the lysophosphatidic acid (LPA) and sphingosine 1-phosphate (S1P) receptors in the late 1990s. Vertebrate LP receptors regulate embryogenesis, vascular development, neurogenesis, uterine development, oocyte survival, immune ce...

journal_title：Journal of lipid research

authors： Skoura A,Hla T

更新日期：2009-04-01 00:00:00

abstract：:The density distribution of electronegative LDL [LDL(-)], a cytotoxic and inflammatory fraction of LDL present in plasma, was studied in 10 normolipemic (NL), 6 FH, and 11 hypertriglyceridemic (HTG) subjects. Six LDL subclasses of increased density (LDL1 to LDL6) were isolated by density-gradient ultracentrifugation (...

journal_title：Journal of lipid research

authors： Sánchez-Quesada JL,Benítez S,Otal C,Franco M,Blanco-Vaca F,Ordóñez-Llanos J

更新日期：2002-05-01 00:00:00

abstract：:Adiponectin, an adipocyte-derived protein, has antiatherogenic and antidiabetic effects, but how it confers the atherogenic effects is not well known. To study the antiatherogenic mechanisms of adiponectin, we examined whether it interacts with atherogenic low density lipoprotein (LDL) to attenuate LDL's atherogenicit...

journal_title：Journal of lipid research

authors： Kakino A,Fujita Y,Ke LY,Chan HC,Tsai MH,Dai CY,Chen CH,Sawamura T

更新日期：2020-11-21 00:00:00

abstract：:Dietary n-3 PUFAs have been shown to attenuate T-cell-mediated inflammation. To investigate whether dietary n-3 PUFAs promote activation-induced cell death (AICD) in CD4+ T-cells induced in vitro to a polarized T-helper1 (Th1) phenotype, C57BL/6 mice were fed diets containing either 5% corn oil (CO; n-6 PUFA control) ...

journal_title：Journal of lipid research

authors： Switzer KC,Fan YY,Wang N,McMurray DN,Chapkin RS

更新日期：2004-08-01 00:00:00

abstract：:Quantitatively and rapidly analyzing lipids is necessary to elucidate their biological functions. Herein, we developed a quantitative method for various lipid classes using supercritical fluid chromatography (SFC) coupled with a charged aerosol detector (CAD), providing high-throughput data analysis to detect a large ...

journal_title：Journal of lipid research

authors： Takeda H,Takahashi M,Hara T,Izumi Y,Bamba T

更新日期：2019-08-01 00:00:00

abstract：:Previous studies have shown that reduced levels of the adipocyte fatty acid binding protein (FABP)4 (AFABP/aP2), result in metabolic improvement including potentiated insulin sensitivity and attenuated atherosclerosis. Mechanistically, pharmacologic or genetic inhibition of FABP4 in macrophages upregulates UCP2, atten...

journal_title：Journal of lipid research

authors： Hertzel AV,Xu H,Downey M,Kvalheim N,Bernlohr DA

更新日期：2017-07-01 00:00:00

abstract：:Several recent reports have examined whether there is a correlation between the presence of some minor alleles of the highly polymorphic apolipoprotein B gene and atherosclerosis and premature heart disease. The present study extends this investigation. A high-resolution method was used to study the allele frequencies...

journal_title：Journal of lipid research

authors： Friedl W,Ludwig EH,Paulweber B,Sandhofer F,McCarthy BJ

更新日期：1990-04-01 00:00:00

abstract：:This review presents an overview of mammalian phospholipid synthesis and the cellular locations of the biochemical activities that produce membrane lipid molecular species. The generalized endoplasmic reticulum compartment is a central site for membrane lipid biogenesis, and examples of the emerging relationships betw...

journal_title：Journal of lipid research

authors： Fagone P,Jackowski S

更新日期：2009-04-01 00:00:00

abstract：:Synthesis of apolipoprotein (apo)E in hepatocytes leads to both secretion and retention in cell surface pools. Inclusion of Brefeldin A to HepG2 cells prompted a rapid decrease of cell surface apoE to about 37% of control values after a 3-h incubation. The t(1/2) for this dynamic pool was estimated to be 15 min. In co...

journal_title：Journal of lipid research

authors： Burgess JW,Marcel YL

更新日期：2001-09-01 00:00:00

abstract：:The sequences necessary for liver-specific expression of the apolipoprotein (apo) E gene have been shown to reside 3' to the gene, within the apoE/C-I/C-II gene cluster, but have not been precisely characterized. Utilizing a transient transfection reporter gene assay based on the apoC-II promoter, we have localized a ...

journal_title：Journal of lipid research

authors： Shachter NS,Zhu Y,Walsh A,Breslow JL,Smith JD

更新日期：1993-10-01 00:00:00

abstract：:Diet-induced changes in high density lipoprotein (HDL) density and size were studied in patas monkeys. When the animals were switched from a moderate fat-low cholesterol diet to a high fat-high cholesterol (HFHC) diet, the plasma apoA-I levels increased initially in all of the animals. The apoA-I levels remained eleva...

journal_title：Journal of lipid research

authors： Melchior GW,Baker HN,Abee CR,Roheim PS

更新日期：1984-09-01 00:00:00

abstract：:The skin surface represents our interface with the external environment, and as such, is our first line of defense against microbial colonization and infection. Lipids at the skin surface are thought to underlie at least part of an antimicrobial barrier. Some of these lipids are synthesized in the epidermis and are ca...

journal_title：Journal of lipid research

authors： Drake DR,Brogden KA,Dawson DV,Wertz PW

更新日期：2008-01-01 00:00:00