The measurement of lysosomal phospholipase A2 activity in plasma.

abstract：:3 alpha,7 alpha-dihydroxy-5 beta-cholestan-26-oic acid (DHCA) and 3 alpha,7 alpha,12 alpha-trihydroxy-5 beta-cholestan-26-oic acid (THCA) are metabolized into chenodeoxycholic acid and cholic acid, respectively, through oxidation and cleavage of the terminal three carbons of the side chain. The present study was desig...

journal_title：Journal of lipid research

authors： Cass OW,Williams GC,Hanson RF

更新日期：1980-02-01 00:00:00

abstract：:In mammals, the esterification of sterols by ACAT plays a critical role in eukaryotic lipid homeostasis. Using the predominant isoform of the yeast ACAT-related enzyme family, Are2p, as a model, we targeted phylogenetically conserved sequences for mutagenesis in order to identify functionally important motifs. Deletio...

journal_title：Journal of lipid research

authors： Guo Z,Cromley D,Billheimer JT,Sturley SL

更新日期：2001-08-01 00:00:00

abstract：:Imidazole catalyzed acylations of lysolipids by acyl-CoAs in water at room temperature and at a pH close to neutrality. In the presence of oleoyl-CoA and either lysophosphatidylcholine, 1-palmitoyl-sn-glycero-3-phosphocholine (LPC); lysophosphatidylglycerol, monoacyl-sn-glycero-3-phosphoglycerol; lysophosphatidyl acid...

journal_title：Journal of lipid research

authors： Testet E,Akermoun M,Shimoji M,Cassagne C,Bessoule JJ

更新日期：2002-07-01 00:00:00

abstract：:Stearoyl-CoA desaturase (SCD) catalyzes the rate-limiting step in the cellular synthesis of monounsaturated fatty acids, mainly oleate (18:1) and palmitoleate (16:1), which are the major monounsaturated fatty acids of membrane phospholipids, cholesteryl esters, waxes, and triglycerides. The mouse expresses three well-...

journal_title：Journal of lipid research

authors： Kim HJ,Miyazaki M,Ntambi JM

更新日期：2002-10-01 00:00:00

abstract：:HDL and its major component, apolipoprotein A-I (apoA-I), play a central role in reverse cholesterol transport. We recently reported the involvement of a glycosylphosphatidylinositol anchor (GPI anchor) in the binding of HDL and apoA-I on human macrophages, and purified an 80 kDa HDL/apoA-I binding protein. In the pre...

journal_title：Journal of lipid research

authors： Matsuyama A,Sakai N,Hiraoka H,Hirano K,Yamashita S

更新日期：2006-01-01 00:00:00

abstract：:Labeled cholesterol esters were incorporated into chylomicrons in vitro by adding an acetone solution of the cholesterol ester to a suspension of washed chylomicrons. The physical and metabolic properties of the added labeled ester were the same as those of cholesterol esters incorporated into chylomicrons in vivo, ex...

journal_title：Journal of lipid research

authors： Quarfordt SH,Goodman DS

更新日期：1966-09-01 00:00:00

abstract：:Triacylglycerols (TG) are the major storage form of energy in eukaryotic organisms and are synthesized primarily by acyl CoA:1,2-diacylglycerol acyltransferase (DGAT) enzymes. In vitro DGAT activity has previously been quantified by measuring the incorporation of either radiolabeled fatty acyl CoA or diacylglycerol (D...

journal_title：Journal of lipid research

authors： McFie PJ,Stone SJ

更新日期：2011-09-01 00:00:00

abstract：:Our goal was to further define the role of LPL gene polymorphisms in coronary heart disease (CHD) risk. We determined the frequencies of three LPL polymorphisms (D9N, N291S, and S447X) in 899 men from the Veterans Affairs HDL Intervention Trial (VA-HIT), a study that examined the potential benefits of increasing HDL w...

journal_title：Journal of lipid research

authors： Brousseau ME,Goldkamp AL,Collins D,Demissie S,Connolly AC,Cupples LA,Ordovas JM,Bloomfield HE,Robins SJ,Schaefer EJ

更新日期：2004-10-01 00:00:00

abstract：:The rate of lipolytic enzyme-catalyzed reactions yielding water-soluble products can be measured by isobaric titration. The method is based upon the measurement of the amount of substrate that must be added to a monalayer to maintain constant surface pressure during the course of the enzymatic reaction. The rate const...

journal_title：Journal of lipid research

authors： Brockman HL,Kézdy FJ,Law JH

更新日期：1975-01-01 00:00:00

abstract：:The direct effect of apolipoprotein (apo) E on cholesteryl ester transfer protein (CETP) activity was studied using lipoproteins from a subject with apoE deficiency (Atherosclerosis. 1991. 88: 15-20) as a model system. The transfer of cholesteryl ester (CE) from discoidal bilayer particles (DBP) to very low density li...

journal_title：Journal of lipid research

authors： Kinoshita M,Arai H,Fukasawa M,Watanabe T,Tsukamoto K,Hashimoto Y,Inoue K,Kurokawa K,Teramoto T

更新日期：1993-02-01 00:00:00

abstract：:Gangliosides and sulfatides (STs) are acidic glycosphingolipids (GSLs) that have one or more sialic acids or sulfate substituents, in addition to neutral sugars, attached to the C-1 hydroxyl group of the ceramide long chain base. TLC is a widely employed and convenient technique for separation and characterization of ...

journal_title：Journal of lipid research

authors： Park H,Zhou Y,Costello CE

更新日期：2014-04-01 00:00:00

abstract：:Subcellular localization of nonesterified cholesterol has been determined in normal human fibroblasts from cultures incubated with human low density lipoprotein (LDL). Nonesterified and esterified cholesterol content of fibroblasts, grown initially in the absence of cholesterol, increased significantly after a 1-hour ...

journal_title：Journal of lipid research

authors： Kruth HS,Blanchette-Mackie J,Avigan J,Gamble W,Vaughan M

更新日期：1982-11-01 00:00:00

abstract：:Membrane-protein interaction plays key roles in a wide variety of biological processes. Although various methods have been employed to measure membrane binding of soluble proteins, a robust high-throughput assay that is universally applicable to all proteins is lacking at present. Here we report a new fluorescence que...

journal_title：Journal of lipid research

authors： Kim H,Afsari HS,Cho W

更新日期：2013-12-01 00:00:00

abstract：:Scavenger receptor class B type I (SR-BI) and ABCA1 are structurally dissimilar cell surface proteins that play key roles in HDL metabolism. SR-BI is a receptor that binds HDL with high affinity and mediates both the selective lipid uptake of cholesteryl esters from lipid-rich HDL to cells and the efflux of unesterifi...

journal_title：Journal of lipid research

authors： Nieland TJ,Chroni A,Fitzgerald ML,Maliga Z,Zannis VI,Kirchhausen T,Krieger M

更新日期：2004-07-01 00:00:00

abstract：:A higher incidence of coronary artery disease is associated with a lower level of HDL-cholesterol. We searched for genetic loci influencing HDL-cholesterol in F2 mice from a cross between MRL/MpJ and SM/J mice. Quantitative trait loci (QTL) mapping revealed one significant HDL QTL (Apoa2 locus), four suggestive QTL on...

journal_title：Journal of lipid research

authors： Leduc MS,Blair RH,Verdugo RA,Tsaih SW,Walsh K,Churchill GA,Paigen B

更新日期：2012-06-01 00:00:00

abstract：:Inflammation has been proposed to impair HDL function and reverse cholesterol transport (RCT). We investigated the effects of inflammation mediated by zymosan, a yeast glucan, on multiple steps along the RCT pathway in vivo and ex vivo. Acute inflammation with 70 mg/kg zymosan impaired RCT to plasma, liver, and feces ...

journal_title：Journal of lipid research

authors： Malik P,Berisha SZ,Santore J,Agatisa-Boyle C,Brubaker G,Smith JD

更新日期：2011-05-01 00:00:00

abstract：:Zinc Finger Protein 202 (ZNF202) is a transcriptional repressor that binds elements found predominantly in genes involved in HDL metabolism. We tested the following hypotheses: 1) frequencies of single-nucleotide polymorphisms (SNPs) and haplotypes in ZNF202 differ between individuals with low and high HDL cholesterol...

journal_title：Journal of lipid research

authors： Stene MC,Frikke-Schmidt R,Nordestgaard BG,Tybjaerg-Hansen A

更新日期：2006-05-01 00:00:00

abstract：:Rat liver microsomes were incubated with the monofunctional aminoreagent fluorescamine. Although the probe easily penetrated the membranes, two pools of phosphatidylethanolamine (PE) could be detected. The first pool rapidly reacted with the probe and comprised 80% of the total PE. The second pool exhibited a very slo...

journal_title：Journal of lipid research

authors： Valtersson C,Filipsson L,Dallner G

更新日期：1986-07-01 00:00:00

abstract：:Lysophosphatidic acid (LPA) is a receptor-active lipid mediator with a broad range of biological effects. Ovarian cancer cells synthesize LPA, which promotes their motility, growth, and survival. We show that a murine homolog of a human protein previously reported to hydrolyze LPA is a highly selective detergent-stimu...

journal_title：Journal of lipid research

authors： Luquain C,Singh A,Wang L,Natarajan V,Morris AJ

更新日期：2003-10-01 00:00:00

abstract：:Different batches of 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) had varying amounts of contaminants which appeared to affect systematic biological studies. This contaminant was separated by silica gel column chromatography followed by high performance liquid chromatography and identified as 1,3-distearoyl-glyce...

journal_title：Journal of lipid research

authors： Ponpipom MM,Bugianesi RL

更新日期：1980-01-01 00:00:00

abstract：:Mutational inactivation of NPC1 largely blocks the movement of LDL-derived cholesterol from the lysosome to the metabolically active, cytosolic pool of sterol that is the substrate for steroid hormone production. Such a block might, in theory, lead to deficiencies in circulating levels of testosterone, progesterone, a...

journal_title：Journal of lipid research

authors： Xie C,Richardson JA,Turley SD,Dietschy JM

更新日期：2006-05-01 00:00:00

abstract：:Phytanic acid (3,7,11,15-tetramethylhexadecanoic acid) is a branched-chain fatty acid derived from dietary sources and broken down in the peroxisome to pristanic acid (2,6,10,14-tetramethylpentadecanoic acid) via alpha-oxidation. Pristanic acid then undergoes beta-oxidation in peroxisomes. Phytanic acid naturally occu...

journal_title：Journal of lipid research

authors： Ferdinandusse S,Rusch H,van Lint AE,Dacremont G,Wanders RJ,Vreken P

更新日期：2002-03-01 00:00:00

abstract：:The mechanisms involved in the development of alcoholic liver disease (ALD) are not well established. We investigated the involvement of acyl-CoA: diacylglycerol acyltransferase 2 (DGAT2) upregulation in mediating hepatic fat accumulation induced by chronic alcohol consumption. Chronic alcohol feeding caused fatty liv...

journal_title：Journal of lipid research

authors： Wang Z,Yao T,Song Z

更新日期：2010-11-01 00:00:00

abstract：:Vitamin A (VA) is stored in tissues predominantly as retinyl esters (REs), which provide substrate for the production of bioactive retinoids. Retinoic acid (RA), a principal metabolite, has been shown to induce postnatal lung development. To better understand lung RE storage, we compared VA (given as retinyl palmitate...

journal_title：Journal of lipid research

authors： Ross AC,Ambalavanan N,Zolfaghari R,Li NQ

更新日期：2006-08-01 00:00:00

abstract：:More than 100 proteins necessary for eukaryotic cell growth, differentiation, and morphology require posttranslational modification by the covalent attachment of an isoprenoid lipid (prenylation). Prenylated proteins include members of the Ras, Rab, and Rho families, lamins, CENPE and CENPF, and the gamma subunit of m...

journal_title：Journal of lipid research

authors： Lane KT,Beese LS

更新日期：2006-04-01 00:00:00

abstract：:ATP-binding cassette transporter A1 (ABCA1) plays a crucial role in apoA-I lipidation, a key step in reverse cholesterol transport. cAMP induces apoA-I binding activity and promotes cellular cholesterol efflux. We investigated the role of the cAMP/protein kinase A (PKA) dependent pathway in the regulation of cellular ...

journal_title：Journal of lipid research

authors： Haidar B,Denis M,Krimbou L,Marcil M,Genest J Jr

更新日期：2002-12-01 00:00:00

abstract：:A variety of methods are currently used to analyze HL and LPL activities in mice. In search of a simple methodology, we analyzed mouse preheparin and postheparin plasma LPL and HL activities using specific polyclonal antibodies raised in rabbit against rat HL (anti-HL) and in goat against rat LPL (anti-LPL). As an alt...

journal_title：Journal of lipid research

authors： Dallinga-Thie GM,Zonneveld-de Boer AJ,van Vark-van der Zee LC,van Haperen R,van Gent T,Jansen H,De Crom R,van Tol A

更新日期：2007-12-01 00:00:00

abstract：:The endocannabinoid 2-arachidonoylglycerol (2-AG) is predominantly biosynthesized by sn-1-diacylglycerol lipase α (DAGL-α) in the CNS. Selective inhibitors of DAGL-α will provide valuable insights in the role of 2-AG in endocannabinoid signaling processes and are potential therapeutics for the treatment of obesity and...

journal_title：Journal of lipid research

authors： van der Wel T,Janssen FJ,Baggelaar MP,Deng H,den Dulk H,Overkleeft HS,van der Stelt M

更新日期：2015-04-01 00:00:00

abstract：:The Delta6-desaturase catalyzes key steps in long-chain polyunsaturated fatty acid biosynthesis. Although the gene coding for this enzyme has been isolated in diverse animal species, the protein structure remains poorly characterized. In this work, rat Delta6-desaturase expressed in COS-7 cells was shown to localize i...

journal_title：Journal of lipid research

authors： Guillou H,D'Andrea S,Rioux V,Barnouin R,Dalaine S,Pedrono F,Jan S,Legrand P

更新日期：2004-01-01 00:00:00

abstract：:A three-step procedure has been developed for the separation of complex mixtures of sterol intermediates in cholesterol biosynthesis. The method has been applied to the separation of sterol intermediates formed from [14C]mevalonate by normal rat hepatocyte culture cells. In Step 1, a short gravity-flow silicic acid co...

journal_title：Journal of lipid research

authors： Hansbury E,Scallen TJ

更新日期：1980-09-01 00:00:00