Role of phospholipase D in agonist-stimulated lysophosphatidic acid synthesis by ovarian cancer cells.

abstract：:Niemann-Pick disease, type C (NP-C), often associated with Niemann-Pick disease, type C1 (NPC1) mutations, is a cholesterol-storage disorder characterized by cellular lipid accumulation, neurodegeneration, and reduced steroid production. To study NPC1 function in vivo, we cloned zebrafish npc1 and analyzed its gene ex...

journal_title：Journal of lipid research

authors： Schwend T,Loucks EJ,Snyder D,Ahlgren SC

更新日期：2011-07-01 00:00:00

abstract：:Described herein are the chemical syntheses of a number of deuterated derivatives of 5 alpha-cholest-8(14)-en-3 beta-ol-15-one. These include the [2,2,3 alpha,4,4,7,7,9 alpha,16,16-2H10]-, [7 alpha,9 alpha,16,16-2H4]-, [7,7,9 alpha,16,16-2H5]-, and [2,2,3 alpha,4,4-2H5]-analogs of the delta 8(14)-15-ketosterol. Also i...

journal_title：Journal of lipid research

authors： St Pyrek J,Wilson WK,Schroepfer GJ Jr

更新日期：1987-11-01 00:00:00

abstract：:Smith-Lemli-Opitz syndrome (SLOS) is a genetic disorder characterized by low plasma cholesterol and high 7-dehydrocholesterol (7-DHC). Synthesis of cholesterol and 7-DHC and its metabolites is regulated by HMG-CoA reductase, whose activity can be measured by 24-h excretion of its product mevalonate. We devised a simpl...

journal_title：Journal of lipid research

authors： Pappu AS,Steiner RD,Connor SL,Flavell DP,Lin DS,Hatcher L,Illingworth DR,Connor WE

更新日期：2002-10-01 00:00:00

abstract：:Chylomicron remnants have been suggested to be involved in the development of atherosclerosis. To investigate the mechanisms of chylomicron remnant-induced atherosclerosis, we prepared cholesterol (Chol)-containing emulsion particles as models for chylomicron remnants. Chol markedly increased the apolipoprotein E (apo...

journal_title：Journal of lipid research

authors： Sakurai A,Morita SY,Wakita K,Deharu Y,Nakano M,Handa T

更新日期：2005-10-01 00:00:00

abstract：:In vitro experiments have demonstrated that exogenous phospholipid transfer protein (PLTP), i.e. purified PLTP added to macrophage cultures, influences ABCA1-mediated cholesterol efflux from macrophages to HDL. To investigate whether PLTP produced by the macrophages (i.e., endogenous PLTP) is also part of this process...

journal_title：Journal of lipid research

authors： Lee-Rueckert M,Vikstedt R,Metso J,Ehnholm C,Kovanen PT,Jauhiainen M

更新日期：2006-08-01 00:00:00

abstract：:Cerebrosides have been isolated from adult human aortic tissue. Each aorta was divided into portions classified as normal, fatty streaks, fibrous plaques, or complicated lesions. The cerebrosides were isolated by Florisil column chromatography, mild alkaline methanolysis, a second Florisil column, and preparative thin...

journal_title：Journal of lipid research

authors： Foote JL,Coles E

更新日期：1968-07-01 00:00:00

abstract：:ABCG1 and ABCG4 are highly homologous members of the ATP binding cassette (ABC) transporter family that regulate cellular cholesterol homeostasis. In adult mice, ABCG1 is known to be expressed in numerous cell types and tissues, whereas ABCG4 expression is limited to the central nervous system (CNS). Here, we show sig...

journal_title：Journal of lipid research

authors： Bojanic DD,Tarr PT,Gale GD,Smith DJ,Bok D,Chen B,Nusinowitz S,Lövgren-Sandblom A,Björkhem I,Edwards PA

更新日期：2010-01-01 00:00:00

abstract：:Five samples from three different types of 1500-year-old Mediterranean amphorae, as well as from a contemporary oil lamp found in the same deposit, were analyzed for the presence of lipid residues. Each sample of finely ground amphorae powder weighed 1-2 g. The abundance of interfering secondary products makes thin-la...

journal_title：Journal of lipid research

authors： Passi S,Rothschild-Boros MC,Fasella P,Nazzaro-Porro M,Whitehouse D

更新日期：1981-07-01 00:00:00

abstract：:Recent studies suggest: that the epidermis and pilosebaceous epithelium are important sites of de novo sterol synthesis, and that the rate of cutaneous cholesterol synthesis does not change with alterations in circulating sterol levels. Since cutaneous sterols may be important for permeability barrier function, we stu...

journal_title：Journal of lipid research

authors： Menon GK,Feingold KR,Moser AH,Brown BE,Elias PM

更新日期：1985-04-01 00:00:00

abstract：:Gap junctions of rat brown adipose tissue (BAT) were studied with the freeze-fracture technique during development. The frequency and the mean area of gap junctions increased after birth, reached a maximal development during the functional phase of the tissue (birth to 3--4 weeks) and decreased while brown adipose tis...

journal_title：Journal of lipid research

authors： Schneider-Picard G,Carpentier JL,Orci L

更新日期：1980-07-01 00:00:00

abstract：:Ceramides play an essential role in modulating immune signaling pathways and proinflammatory cytokine production in response to infectious pathogens, stress stimuli, or chemotherapeutic drugs. In this study, we demonstrated that Aggregatibacter actinomycetemcomitans, the pathogen for aggressive periodontitis, induced ...

journal_title：Journal of lipid research

authors： Yu H,Valerio M,Bielawski J

更新日期：2013-01-01 00:00:00

abstract：:Protein palmitoylation refers to the posttranslational addition of a 16 carbon fatty acid to the side chain of cysteine, forming a thioester linkage. This acyl modification is readily reversible, providing a potential regulatory mechanism to mediate protein-membrane interactions and subcellular trafficking of proteins...

journal_title：Journal of lipid research

authors： Mitchell DA,Vasudevan A,Linder ME,Deschenes RJ

更新日期：2006-06-01 00:00:00

abstract：:The basic protein protamine strongly inhibited hydrolysis of triolein emulsified with soybean phosphatidylcholine (PC) by pancreatic and carboxylester lipases; 10 micrograms/ml protamine, about 1000 times lower than the concentration of bovine serum albumin for the same effect, inhibited triolein hydrolysis completely...

journal_title：Journal of lipid research

authors： Tsujita T,Matsuura Y,Okuda H

更新日期：1996-07-01 00:00:00

abstract：:Apheresis is a treatment option for patients with severe hypercholesterolemia and coronary artery disease. It is, however, unknown whether such therapy changes kinetic parameters of lipoprotein metabolism, such as apolipoprotein B (apoB) secretion rates, conversion rates, and fractional catabolic rates (FCR). We studi...

journal_title：Journal of lipid research

authors： Parhofer KG,Barrett PH,Demant T,Richter WO,Schwandt P

更新日期：1996-11-01 00:00:00

abstract：:Common forms of metabolic and cardiovascular diseases involve the interplay of numerous genes as well as important environmental factors. Traditional biochemical and genetic approaches generally attempt to dissect these diseases one gene at a time, for example, by analysis of Mendelian forms or genetically engineered ...

journal_title：Journal of lipid research

authors： Wu S,Lusis AJ,Drake TA

更新日期：2009-04-01 00:00:00

abstract：:MDCO-216, a complex of dimeric recombinant apoA-IMilano (apoA-IM) and palmitoyl-oleoyl-phosphatidylcholine (POPC), was administered to cynomolgus monkeys at 30, 100, and 300 mg/kg every other day for a total of 21 infusions, and effects on lipids, (apo)lipoproteins, and ex-vivo cholesterol efflux capacity were monitor...

journal_title：Journal of lipid research

authors： Kempen HJ,Gomaraschi M,Bellibas SE,Plassmann S,Zerler B,Collins HL,Adelman SJ,Calabresi L,Wijngaard PL

更新日期：2013-09-01 00:00:00

abstract：:Macrophages and arterial chondroitin sulfate proteoglycans (CSPG) are probably associated with extracellular and intracellular lipoprotein deposition during atherogenesis. We found that human arterial CSPG can be used to select subclasses from low density lipoprotein (LDL) with different structural properties and capa...

journal_title：Journal of lipid research

authors： Hurt-Camejo E,Camejo G,Rosengren B,Lopez F,Wiklund O,Bondjers G

更新日期：1990-08-01 00:00:00

abstract：:We have reported the use of a retroviral vector to introduce the low density lipoprotein (LDL) receptor gene into receptor-deficient Watanabe heritable hyperlipidemic (WHHL) rabbit fibroblasts (Miyanohara, A., M. F. Sharkey, D. Steinberg, J. L. Witztum, and T. Friedmann. 1988. Proc. Natl. Acad. Sci. USA. 85: 6538-6542...

journal_title：Journal of lipid research

authors： Sharkey MF,Miyanohara A,Elam RL,Friedmann T,Witztum JL

更新日期：1990-12-01 00:00:00

abstract：:Supernatant protein factor (SPF) and anionic phospholipids such as phosphatidylglycerol (PG) stimulate squalene epoxidase activity in rat liver microsomes by promoting [3H]squalene uptake as well as substrate translocation (Chin, J., and K. Bloch. 1984. J. Biol. Chem. 259: 11735-11738). This process is postulated to b...

journal_title：Journal of lipid research

authors： Chin J,Bloch K

更新日期：1985-07-01 00:00:00

abstract：:Origins of hyperlipidemia and cholestasis that occur during pregnancy were investigated by examining expression of key elements related to plasma and hepatic cholesterol metabolism during pregnancy, lactation, and post-lactation in the rat model. Among major findings were: during pregnancy, the activities of hepatic 3...

journal_title：Journal of lipid research

authors： Smith JL,Lear SR,Forte TM,Ko W,Massimi M,Erickson SK

更新日期：1998-11-01 00:00:00

abstract：:In addition to their classical roles as detergents to aid in the process of digestion, bile acids have been identified as important signaling molecules that function through various nuclear and G protein-coupled receptors to regulate a myriad of cellular and molecular functions across both metabolic and nonmetabolic p...

journal_title：Journal of lipid research

authors： Dawson PA,Karpen SJ

更新日期：2015-06-01 00:00:00

abstract：:Maintaining cellular lipid homeostasis is crucial to oxidative tissues, and it becomes compromised in obesity. Lipid droplets (LD) play a central role in lipid homeostasis by mediating fatty acid (FA) storage in the form of triglyceride, thereby lowering intracellular levels of lipids that mediate cellular lipotoxicit...

journal_title：Journal of lipid research

authors： Wang H,Sreenivasan U,Hu H,Saladino A,Polster BM,Lund LM,Gong DW,Stanley WC,Sztalryd C

更新日期：2011-12-01 00:00:00

abstract：:The group IVA calcium-dependent cytosolic phospholipase A2 (cPLA2α) enzyme directs a complex "eicosanoid storm" that accompanies the tissue response to injury. cPLA2α and its downstream eicosanoid mediators are also implicated in the pathogenesis of fibrosis in many organs, including the kidney. We aimed to determine ...

journal_title：Journal of lipid research

authors： Montford JR,Lehman AMB,Bauer CD,Klawitter J,Klawitter J,Poczobutt JM,Scobey M,Weiser-Evans M,Nemenoff RA,Furgeson SB

更新日期：2018-02-01 00:00:00

abstract：:Various detergents can be used to dissolve phospholipids, resulting in very narrow 31PNMR resonances. The resonances are well resolved, allowing identification and quantitative analysis of phospholipids in a mixture. The chemical shift depends strongly on pH, reflecting changes in the state of ionization of the phosph...

journal_title：Journal of lipid research

authors： London E,Feigenson GW

更新日期：1979-03-01 00:00:00

abstract：:The relative rates of hydrolysis of the secondary ester in glycerol 1,3-benzylidene 2-oleate and in glycerol 1,3-dihexadecyl ether 2-oleate, and of the primary and secondary esters in triolein were determined. Both unaltered and selectively inactivated rat pancreatic juice were used as sources of enzyme. It was found ...

journal_title：Journal of lipid research

authors： Mattson FH,Volpenhein RA

更新日期：1968-01-01 00:00:00

abstract：:Due to conflicting reports concerning the relationship between phospholipid transfer protein (PLTP) activity and mass in plasma, the protein concentration and activity of PLTP were assessed in fractions isolated by fast protein liquid chromatography from the plasma of healthy normolipidemic individuals. Using both pol...

journal_title：Journal of lipid research

authors： Murdoch SJ,Wolfbauer G,Kennedy H,Marcovina SM,Carr MC,Albers JJ

更新日期：2002-02-01 00:00:00

abstract：:Spectrophotometric techniques for determining the activities of lipases, lysophospholipases, and phospholipases are reviewed. These methods involve the use of thioester substrate analogs as well as omega-nitrophenyl derivatives of the corresponding lipids. The most promising results are obtained with the thioester sub...

journal_title：Journal of lipid research

authors： Farooqui AA,Taylor WA,Pendley CE 2nd,Cox JW,Horrocks LA

更新日期：1984-12-15 00:00:00

abstract：:Long-chain fatty acids are the most important substrates for the heart. In addition, they have been shown to affect signalling pathways and gene expression. To explore the effects of long-chain fatty acids on cardiac gene expression, neonatal rat ventricular myocytes were cultured for 48 h with either glucose (10 mm),...

journal_title：Journal of lipid research

authors： van der Lee KA,Vork MM,De Vries JE,Willemsen PH,Glatz JF,Reneman RS,Van der Vusse GJ,Van Bilsen M

更新日期：2000-01-01 00:00:00

abstract：:The major high density lipoprotein (HDL) apolipoprotein, apoA-I, was knocked out by gene targeting in ES cells to provide a model for the study of HDL metabolism and its relationship to plasma and tissue cholesterol metabolism. HDL and non-HDL cholesterol (HDL-C) were reduced in apoA-I-deficient mice. Feeding a high f...

journal_title：Journal of lipid research

authors： Plump AS,Azrolan N,Odaka H,Wu L,Jiang X,Tall A,Eisenberg S,Breslow JL

更新日期：1997-05-01 00:00:00

abstract：:Mice with a targeted mutation of 3beta-hydroxysterol Delta(7)-reductase (Dhcr7) that cannot convert 7-dehydrocholesterol to cholesterol were used to identify the origin of fetal sterols. Because their heterozygous mothers synthesize cholesterol normally, virtually all sterols found in a Dhcr7 knockout fetus having a D...

journal_title：Journal of lipid research

authors： Tint GS,Yu H,Shang Q,Xu G,Patel SB

更新日期：2006-07-01 00:00:00