Abstract:
:Vitamin A-transporting protein in chicken plasma was purified by column chromatography on DEAE-Sephadex and Sephadex G-100; the protein formed a complex of retinol-binding protein (RBP) with prealbumin (PA). The molecular weight of the 1:1 molar complex was estimated to be 76,000 by gel filtration, and the sedimentation coefficient (S20,W) was found to be 5.2 S. RBP and PA were dissociated from the purified complex by means of CM-Sephadex column chromatography. Purified RBP contained 1 mole of vitamin A bound per mole of RBP. The molecular weight of RBP was determined to be 20,000 by gel filtration on Sephadex G-75, 19,000 by SDS-disc gel electrophoresis, and 20,500 by sedimentation equilibrium analysis. The S20,W was calculated to be 2.0 S. The molecular weight of PA was determined to be 56,000 by gel filtration, 52,000 by sedimentation equilibrium analysis, and 13,000 by SDS-disc gel electrophoresis. The S20,W was calculated to be 3.9 S. From these findings it was concluded that PA consists of four subunits, each with a molecular weight of approximately 13,000. Peptide mapping experiments suggested that the subunits were identical. No carbohydrates were detected in either RBP or PA. Chicken RBP and PA were immunologically distinct from those of human and rat. It is well established that vitamin A is transported bound to a specific plasma protein, retinol-binding protein (RBP), in both man (1,2) and rat (3). Purified human and rat plasma RBP have a single binding site for one molecule of retinol, alpha mobility on disc gel electrophoresis, and a molecular weight of approximately 20,000. In both species, RBP forms a tight complex with plasma prealbumin (PA) and normally circulates as a 1:1 molar protein-protein complex with PA (1-5). Despite these similarities, no immunological cross-reactivity between human and rat RBP has been observed (3,6). The present study was undertaken to explore whether or not a similar transport system for vitamin A exists in the chicken, a nonmammalian vertebrate. During the course of this study, Mokady and Tal (7) reported the isolation of RBP from chicken plasma and some physicochemical properties, e.g., a molecular weight of about 19,000. On the other hand, Muto, Smith, and Goodman (6) had already observed that the molecular weight of vitamin A-containing protein in fresh chicken plasma is approximately 60,000-80,000, as determined by gel filtration. However, no convincing information is available regarding an entire system of vitamin A transport in chicken plasma. We now describe procedures for the isolation of the RBA-PA complex of chicken plasma and the dissociation into the component proteins, RBP and PA. We also describe in detail the physicochemical properties of the individual proteins. It is also clearly demonstrated that chicken RBP and PA are immunologically distinct and different from the respective proteins in man and rat. Moreover, purified chicken PA appears to be a tetramer of four identical subunits and is thus similar to human and rat PA.
journal_name
J Lipid Resjournal_title
Journal of lipid researchauthors
Abe T,Muto Y,Hosoya Nsubject
Has Abstractpub_date
1975-05-01 00:00:00pages
200-10issue
3eissn
0022-2275issn
1539-7262journal_volume
16pub_type
杂志文章abstract::In promoting oxidation of 0.02 m potassium linoleate in a buffered (pH 7.0) aqueous dispersion at 37 degrees C, ascorbic acid at low concentrations (1.8 x 10(-6) and 1.8 x 10(-5) m) in combination with copper (1.3 x 10(-7) to 1.3 x 10(-3) m) had greater catalytic activity than the additive activity of the two catalyst...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1969-09-01 00:00:00
abstract::Cyclophosphamide injection into the fasted rabbit induces a hypertriglyceridemia (4.6 mM vs. 0.8 mM in controls) and a defect of lipoprotein lipase (LPL), as measured in post-heparin plasma (PHP). In contrast, administration of the drug into fed animals tends to increase PHP-LPL. The effects of cyclophosphamide on LPL...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1993-01-01 00:00:00
abstract::The ability to exogenously present cell-surface receptors in high-affinity conformations in a synthetic system offers an opportunity to provide host cells with protection from pathogenic toxins. This strategy requires improvement of the synthetic receptor binding affinity against its native counterpart, particularly w...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M007401
更新日期:2010-09-01 00:00:00
abstract::Naturally occurring derivatives of arachidonic acid are potent agonists for the nuclear hormone receptor peroxisome proliferator-activated receptor gamma (PPARgamma) and block cancer cell proliferation through the induction of apoptosis. We have previously reported that induction of apoptosis using cyclopentenone pros...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.m200224-jlr200
更新日期:2002-11-01 00:00:00
abstract::The role of phosphodiesterase in glucagon resistance of large adipocytes was investigated. A comparison was made of phosphodiesterase activities of homogenates prepared from isolated small (mean diameter approximately 45 micro m) and large (mean diameter approximately 78 micro m) adipocytes, using various concentratio...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1974-01-01 00:00:00
abstract::Studies on plasma triglycerides (TG) were performed in 10 nonobese and 16 obese patients with hypothyroidism and in 13 with hyperthyroidism. Nonobese, hypothyroid patients generally had normal levels of TG, but obese patients often had hypertriglyceridemia. In most hypothyroid patients 1-thyroxine treatment lowered pl...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1981-02-01 00:00:00
abstract::Mixed background SHP(-/-) mice are resistant to diet-induced obesity due to increased energy expenditure caused by enhanced PGC-1α expression in brown adipocytes. However, congenic SHP(-/-) mice on the C57BL/6 background showed normal expression of PGC-1α and other genes involved in brown adipose tissue thermogenesis....
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M016048
更新日期:2011-12-01 00:00:00
abstract::Lipoprotein (a) [Lp(a)] is a highly atherogenic lipid particle. Although earlier reports suggested that Lp(a) levels are mostly determined by genetic factors, several recent studies have revealed that Lp(a) induction is also caused by chronic inflammation. Therefore, we aimed to examine whether cytokine blockade by mo...
journal_title:Journal of lipid research
pub_type: 临床试验,杂志文章
doi:10.1194/jlr.P052209
更新日期:2015-05-01 00:00:00
abstract::Prostaglandin (PG)E(2) is relevant in tumor biology, and interactions between tumor and stroma cells dramatically influence tumor progression. We tested the hypothesis that cross-talk between head and neck squamous cell carcinoma (HNSCC) cells and fibroblasts could substantially enhance PGE(2) biosynthesis. We observe...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M019695
更新日期:2012-04-01 00:00:00
abstract::Boiled coffee contains an unidentified lipid that raises serum cholesterol. We studied the effects of the ingestion of coffee oil fractions of increasing purity in volunteers in order to identify the cholesterol-raising factor. In 15 volunteers who ingested 0.75 g/d of a non-triglyceride-fraction from coffee oil for 4...
journal_title:Journal of lipid research
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:1994-04-01 00:00:00
abstract::Distinct isoforms of long-chain acyl-CoA synthetases (ACSLs) may partition fatty acids toward specific metabolic cellular pathways. For each of the five members of the rat ACSL family, we analyzed tissue mRNA distributions, and we correlated the mRNA, protein, and activity of ACSL1 and ACSL4 after fasting and refeedin...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M600150-JLR200
更新日期:2006-09-01 00:00:00
abstract::Percent cholesterol absorption was measured in 94 normal subjects aged 17- 80 years while consuming diets generally low in cholesterol (mean intake = 226 +/- 126 mg/day). A new dual stable isotope method was used where a cholesterol tracer containing 6 extra mass units was given intravenously and another tracer with 5...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1999-02-01 00:00:00
abstract::The behavior of human omental fat cells in vitro has been examined in order to define conditions under which glucose is converted to glyceride-glycerol and glyceride fatty acids. Synthesis of glyceride fatty acids from glucose reached maximal rates only after several hours of incubation in Krebs-Henseleit bicarbonate ...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1969-05-01 00:00:00
abstract::In studies of cebus monkey plasma lipoproteins, we have used an ultracentrifugally generated density gradient to isolate two distinct species of low density lipoproteins (LDL). Compositional analyses revealed that each of the ultracentrifugally isolated fractions was enriched in cholesteryl esters and contained a sing...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1989-05-01 00:00:00
abstract::Cholesterol-3-(3)H,4-(14)C was injected intravenously in man and its transformation to cholestanol was studied. From the (3)H: (14)C ratios in cholestanol isolated from blood, evidence for the participation of a ketonic intermediate in the conversion was obtained. In a second subject given cholestanol-3-(3)H,4-(14)C t...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1967-01-01 00:00:00
abstract::Bile acids labeled with 3H on the sterol nucleus lose a substantial fraction of label during enterohepatic cycling and conversion to secondary bile acids. We tested the isotopic stability of a side-chain 3H label, [22,23-3H]cholic acid in humans. The 3H-labeled compound was administered simultaneously with [24-14C]cho...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1996-02-01 00:00:00
abstract::Origins of hyperlipidemia and cholestasis that occur during pregnancy were investigated by examining expression of key elements related to plasma and hepatic cholesterol metabolism during pregnancy, lactation, and post-lactation in the rat model. Among major findings were: during pregnancy, the activities of hepatic 3...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1998-11-01 00:00:00
abstract::Two novel mutations of the low density lipoprotein (LDL)-receptor gene were found in two Italian familial hypercholesterolemia (FH)-heterozygotes. The first mutation was an 18 nucleotide duplication in exon 8 which is preceded by an A-->T transversion. The translation product of the mutant allele was predicted to be a...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1998-07-01 00:00:00
abstract::The enzyme 1-acylglycerol-3-phosphate-O-acyltransferase (AGPAT) converts lysophosphatidic acid (LPA) to phosphatidic acid (PA). In this study, we show enzymatic properties, tissue distribution, and subcellular localization of human AGPAT3 and AGPAT5. In cells overexpressing these isoforms, the proteins were detected i...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M007575
更新日期:2011-03-01 00:00:00
abstract::Oxidized low density lipoprotein (Ox-LDL) has a plethora of components that are not present in native LDL. Their presence and quantity depends on the nature, type, and extent of oxidation. Lipids esterified to oxidized fatty acids are the major components formed during the early phase of oxidation and these show a num...
journal_title:Journal of lipid research
pub_type: 杂志文章,评审
doi:
更新日期:1999-12-01 00:00:00
abstract::Adipogenic differentiation is a highly regulated process that is necessary for metabolic homeostasis and nutrient sensing. The expression of PPARγ and the subsequent activation of adipogenic genes is critical for the process. In this study, we identified lanthionine synthetase C-like protein 2 (LanCL2) as a positive r...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M085274
更新日期:2018-08-01 00:00:00
abstract::The subcellular and submitochondrial localization of CTP:phosphatidate cytidylyltransferase is altered in the Morris 7777 hepatoma. Mitochondria in this poorly differentiated tumor are the principal sites of CDP-diacylglycerol synthesis, in contrast to normal rat liver where the endoplasmic reticulum is most active. T...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1978-07-01 00:00:00
abstract::The ATP binding cassette (ABC) half-transporters ABCG5 and ABCG8 facilitate biliary and intestinal removal of neutral sterols. Here, we identify a binding site for the orphan nuclear receptor liver receptor homolog-1 (LRH-1) at nt 134-142 of the ABCG5/ABCG8 intergenic region necessary for the activity of both the ABCG...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.C400002-JLR200
更新日期:2004-07-01 00:00:00
abstract::We designed a rapid method for determining mRNA content of cholesterol biosynthesis enzymes and LDL receptor (LDLR) using a ribonuclease protection assay (RPA). 32P-labeled cRNA fragments for genes of human LDLR and the enzymes HMG-CoA synthase (HMGS), HMG-CoA reductase (HMGR), mevalonate kinase (MK), farnesyl pyropho...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1995-09-01 00:00:00
abstract::The antitumor effect of daily individual administration of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) (2 g/kg body weight) in Balb/c mice bearing a transplantable thymoma was investigated. Mice received oleic acid (control group), EPA and DHA ethyl esters starting 10 days before tumor inoculation. Anal...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:2000-02-01 00:00:00
abstract::High plasma cholesterol levels are found in several metabolic disorders and their reductions are advocated to reduce the risk of atherosclerosis. A way to lower plasma lipids is to curtail lipoprotein production; however, this is associated with steatosis. We previously showed that microRNA (miR)-30c lowers diet-induc...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M081299
更新日期:2018-01-01 00:00:00
abstract::We analysed the sensitivity to beta-adrenoceptor agonists in epididymal adipose cells from rats submitted to a stress protocol previously reported to induce alterations in sensitivity to catecholamines in cardiac tissue from rats. Food intake and body weight were lower, whereas adipocytes basal lipolysis was higher (c...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1999-09-01 00:00:00
abstract::Three pentadecynoic acids, with the triple bond in the 8-, 9-, and 10-positions, have been synthesized on a gram scale in over-all yield of 65% by refinements of the five-step Ahmad-Strong method; isolation of intermediates was shown to be unnecessary prior to purification of the acetylenic nitriles by column chromato...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1969-11-01 00:00:00
abstract::The quantitative isolation of total glycosphingolipids from crude lipid extracts without contamination from other lipid classes is described. The method consists of (a) acetylation of total lipids with pyridine and acetic anhydride, (b) separation of acetylated glycolipids from nonglycolipids on a magnesia-silica gel ...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1971-03-01 00:00:00
abstract::Chiral phase high performance liquid chromatographic resolution of sn-1,2(2,3)- and X-1,3-diacylglycerols generated by partial Grignard degradation from natural triacylglycerols was carried out using a chiral column (25 cm x 4.6 mm i.d.) containing (R)-(+)-1-(1-napthyl)ethylamine polymer chemically bonded to 300A wide...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1990-09-01 00:00:00