Abstract:
:An amino group of phosphatidylethanolamine (PE) is considered as a target for nonenzymatic glycation, and the potential involvement of lipid glycation in the pathogenesis of diabetic complications has generated interest. However, unlike an early glycation product of PE (Amadori-PE), the occurrence and roles of advanced glycation end products of PE (AGE-PE) in vivo have been unclear. Here, we developed an LC-MS/MS method for the analysis of AGE-PE [carboxymethyl-PE (CM-PE) and carboxyethyl-PE (CE-PE)]. Collision-induced dissociation of CM-PE and CE-PE produced characteristic ions, permitting neutral loss scanning (NLS) and multiple reaction monitoring (MRM) of AGE-PE. By NLS analysis, a series of AGE-PE molecular species was detected in human erythrocytes and blood plasma. In LC-MS/MS analysis, MRM enabled the separation and determination of the predominant AGE-PE species. Between healthy subjects and diabetic patients, no significant differences were observed in AGE-PE concentrations in erythrocytes and plasma, whereas Amadori-PE concentrations were higher in diabetic patients. These results provide direct evidence for the presence of AGE-PE in human blood, and indicated that, compared with Amadori-PE, AGE-PE is less likely to be accumulated in diabetic blood. The presently developed LC-MS/MS method appears to be a powerful tool for understanding in vivo lipid glycation and its pathophysiological consequence.
journal_name
J Lipid Resjournal_title
Journal of lipid researchauthors
Shoji N,Nakagawa K,Asai A,Fujita I,Hashiura A,Nakajima Y,Oikawa S,Miyazawa Tdoi
10.1194/jlr.D004564subject
Has Abstractpub_date
2010-08-01 00:00:00pages
2445-53issue
8eissn
0022-2275issn
1539-7262pii
jlr.D004564journal_volume
51pub_type
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journal_title:Journal of lipid research
pub_type: 杂志文章
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更新日期:1968-09-01 00:00:00
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journal_title:Journal of lipid research
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journal_title:Journal of lipid research
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journal_title:Journal of lipid research
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doi:
更新日期:1997-01-01 00:00:00
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journal_title:Journal of lipid research
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journal_title:Journal of lipid research
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doi:
更新日期:1966-01-01 00:00:00
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journal_title:Journal of lipid research
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journal_title:Journal of lipid research
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journal_title:Journal of lipid research
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journal_title:Journal of lipid research
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