Abstract:
:As microsomal triglyceride transfer protein (MTP) is required for the assembly and secretion of apoB-containing lipoproteins by intestinal epithelial cells, the characterization and regulation of MTP in human intestinal cells, CaCo-2, was studied. CaCo-2 cells express MTP mRNA of 4.2 kb and a 97 kDa subunit of MTP protein. Similar to the expression of apoB mRNA, MTP mRNA expression was dependent upon cell differentiation and was directly related to the ability of the cells to assemble and secrete apoB-containing lipoproteins. MTP mRNA expression was highest in fully differentiated cells, with small but detectable amounts found in undifferentiated cells. Under conditions of increased apoB secretion by oleic acid, phosphatidylcholine, or lysophosphatidylcholine, MTP mass, MTP activity, and MTP gene expressions were not altered. In cells treated with calcium ionophore or phorbol 12-myristate 13-acetate, no relationship could be established between apoB secretion and MTP mRNA or activity. Similarly, in cells treated with sphingomyelinase, trifluoperazine, verapamil, okadaic acid, vanadate, or monensin, agents that decrease apoB secretion, no corresponding decrease in MTP activity or mass was observed. The results suggest that the various mediators of apoB secretion alter steps in lipoprotein assembly and secretion that are not dependent on MTP activity. CaCo-2 cells have an abundant supply of MTP for the assembly of lipoproteins when apoB secretion is stimulated by dietary lipids.
journal_name
J Lipid Resjournal_title
Journal of lipid researchauthors
Mathur SN,Born E,Murthy S,Field FJsubject
Has Abstractpub_date
1997-01-01 00:00:00pages
61-7issue
1eissn
0022-2275issn
1539-7262journal_volume
38pub_type
杂志文章abstract::The objectives of this study were to characterize the effects of plasma lipoproteins on prostacyclin (PGI2) production by the Langendorff-perfused rabbit heart, and to determine the mechanism of lipoprotein-induced cardiac PGI2 production. PGI2 production by perfused rabbit hearts was stimulated by injections of rabbi...
journal_title:Journal of lipid research
pub_type: 杂志文章
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journal_title:Journal of lipid research
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journal_title:Journal of lipid research
pub_type: 杂志文章
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更新日期:1999-04-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
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更新日期:1980-09-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M700060-JLR200
更新日期:2007-09-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1971-11-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M400130-JLR200
更新日期:2004-08-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
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journal_title:Journal of lipid research
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1981-07-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1991-09-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
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journal_title:Journal of lipid research
pub_type: 杂志文章
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更新日期:1994-06-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1996-12-01 00:00:00
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pub_type: 杂志文章,随机对照试验
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journal_title:Journal of lipid research
pub_type: 杂志文章
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更新日期:1999-07-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
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journal_title:Journal of lipid research
pub_type: 杂志文章
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更新日期:1998-04-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
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