Contribution of 3-hydroxyisobutyrate to the measurement of 3-hydroxybutyrate in human plasma: comparison of enzymatic and gas-liquid chromatography-mass spectrometry assays in normal and in diabetic subjects.

abstract：:A strategy has been developed for archaebacterial lipid analysis which provides three times the information to describe archaebacterial isolates and is compatible with simultaneous eubacterial/eukaryotic lipid analysis of environmental samples. Eubacterial and micro-eukaryotic biomass, community structure, and nutriti...

journal_title：Journal of lipid research

authors： Hedrick DB,Guckert JB,White DC

更新日期：1991-04-01 00:00:00

abstract：:Lipids were extracted from alfalfa samples collected at intervals over the growing season and were fractionated to yield pure sulfolipid. In the sulfolipid and in a phospholipid fraction the major fatty acids were palmitic, linolenic, and linoleic, of which the palmitic acid increased in proportion during the season w...

journal_title：Journal of lipid research

authors： Klopfenstein WE,Shigley JW

更新日期：1967-07-01 00:00:00

abstract：:The percentage of saturated cholesteryl esters (CEs) synthesized by human LCAT is several times higher than expected from the sn-2 acyl composition of plasma phosphatidylcholine (PC), whereas the synthesis of 20:4 CE and 22:6 CE is much lower than expected. To explain these discrepancies, we proposed that LCAT transfe...

journal_title：Journal of lipid research

authors： Subbaiah PV,Sowa JM,Davidson MH

更新日期：2004-12-01 00:00:00

abstract：:This study was undertaken to determine the mechanisms that regulate cholesterol synthesis in vivo and to quantitate the relative importance of the liver and extrahepatic tissues as sites for sterol synthesis. Rats were administered [3H]water intravenously and killed 1 hour later. The amount of [3H]water incorporated i...

journal_title：Journal of lipid research

authors： Jeske DJ,Dietschy JM

更新日期：1980-03-01 00:00:00

abstract：:Subnormal HDL-cholesterol (HDL-C) and apolipoprotein (apo)AI levels are characteristic of familial hypercholesterolemia (FH), reflecting perturbed intravascular metabolism with compositional anomalies in HDL particles, including apoE enrichment. Does LDL-apheresis, which reduces HDL-cholesterol, apoAI, and apoE by ads...

journal_title：Journal of lipid research

authors： Orsoni A,Saheb S,Levels JH,Dallinga-Thie G,Atassi M,Bittar R,Robillard P,Bruckert E,Kontush A,Carrié A,Chapman MJ

更新日期：2011-12-01 00:00:00

abstract：:Autophagy is a catabolic process involved in maintaining energy and organelle homeostasis. The relationship between obesity and the regulation of autophagy is cell type specific. Despite adverse consequences of obesity on cardiac structure and function, the contribution of altered cardiac autophagy in response to fatt...

journal_title：Journal of lipid research

authors： Jaishy B,Zhang Q,Chung HS,Riehle C,Soto J,Jenkins S,Abel P,Cowart LA,Van Eyk JE,Abel ED

更新日期：2015-03-01 00:00:00

abstract：:We analyzed Niemann-Pick type C disease 1 (NPC1) gene in 12 patients with Niemann-Pick type C disease by sequencing both cDNA obtained from fibroblasts and genomic DNA. All the patients were compound heterozygotes. We found 15 mutations, eight of which previously unreported. The comparison of cDNA and genomic DNA reve...

journal_title：Journal of lipid research

authors： Tarugi P,Ballarini G,Bembi B,Battisti C,Palmeri S,Panzani F,Di Leo E,Martini C,Federico A,Calandra S

更新日期：2002-11-01 00:00:00

abstract：:Eubacterium sp. 144 dehydroxylated 16 alpha-hydroxy-progesterone; however, the expected intermediate, delta 16-progesterone, did not accumulate to significant concentrations in the culture medium. Moreover, the final end product of this biotransformation, 17 alpha-progesterone, was produced at a very slow rate. It was...

journal_title：Journal of lipid research

authors： Glass TL,Winter J,Bokkenheuser VD,Hylemon PB

更新日期：1982-02-01 00:00:00

abstract：:In eukaryotes, many cell surface proteins are attached to the plasma membrane via a glycolipid glycosylphosphatidylinositol (GPI) anchor. GPI-anchored proteins (GPI-APs) receive the GPI anchor as a conserved posttranslational modification in the lumen of the endoplasmic reticulum (ER). After anchor attachment, the GPI...

journal_title：Journal of lipid research

authors： Muñiz M,Riezman H

更新日期：2016-03-01 00:00:00

abstract：:More than 100 proteins necessary for eukaryotic cell growth, differentiation, and morphology require posttranslational modification by the covalent attachment of an isoprenoid lipid (prenylation). Prenylated proteins include members of the Ras, Rab, and Rho families, lamins, CENPE and CENPF, and the gamma subunit of m...

journal_title：Journal of lipid research

authors： Lane KT,Beese LS

更新日期：2006-04-01 00:00:00

abstract：:Glycosylphosphatidylinositol (GPI) enriches GPI-anchored proteins (GPI-AP) in lipid rafts by intimate interaction of its lipid moiety with sphingolipids and cholesterol. In addition to such lipid-lipid interactions, it has been reported that GPI may interact with protein moiety linked to GPI and affect protein conform...

journal_title：Journal of lipid research

authors： Seong J,Wang Y,Kinoshita T,Maeda Y

更新日期：2013-04-01 00:00:00

abstract：:We have defined the lipid composition of copper-oxidized LDL (Cu-oxLDL) and a macrophage-foam cell model generated by the uptake of this modified lipoprotein. An HPLC method previously developed by our group for the measurement of lipid oxidation products of LDL was extended to permit the analysis of an array of 7-ket...

journal_title：Journal of lipid research

authors： Brown AJ,Dean RT,Jessup W

更新日期：1996-02-01 00:00:00

abstract：:Gel-permeation chromatography on Sephadex LH-20, using ethanol as eluent, permits the resolution of neutral hydroxy lipids according to molecular size. The influence of molecular shape, functional groups, chain lengths, and degree of unsaturation, as well as the effect of the eluent on the elution pattern are discusse...

journal_title：Journal of lipid research

authors： Calderon M,Baumann WJ

更新日期：1970-03-01 00:00:00

abstract：:In studies of cebus monkey plasma lipoproteins, we have used an ultracentrifugally generated density gradient to isolate two distinct species of low density lipoproteins (LDL). Compositional analyses revealed that each of the ultracentrifugally isolated fractions was enriched in cholesteryl esters and contained a sing...

journal_title：Journal of lipid research

authors： Puppione DL,Nicolosi RJ,Kowala MC,Schumaker VN

更新日期：1989-05-01 00:00:00

abstract：:Using a radiometric assay with glycochenodeoxycholic acid as substrate, bile acid:3'-phosphoadenosine-5'-phosphosulfate sulfotransferase activity was found in 105,000 g supernatant fractions of liver, proximal intestine, and adrenal gland homogenates from adult hamsters. Optimum conditions for measurement of the hepat...

journal_title：Journal of lipid research

authors： Barnes S,Burhol PG,Zander R,Haggstrom G,Settine RL,Hirschowitz BI

更新日期：1979-11-01 00:00:00

abstract：:The in vivo formation of the sulfate ester of glycolithocholate is a critical step in the elimination of this hepatotoxic bile salt. Rhesus monkeys fed chenodeoxycholate or ursodeoxycholate, the precursors of lithocholate, develop frank cirrhosis in association with accumulation of nonsulfated glycolithocholate in bil...

journal_title：Journal of lipid research

authors： Barnes S,Waldrop R,Crenshaw J,King RJ,Taylor KB

更新日期：1986-11-01 00:00:00

abstract：:Mice with a targeted mutation of 3beta-hydroxysterol Delta(7)-reductase (Dhcr7) that cannot convert 7-dehydrocholesterol to cholesterol were used to identify the origin of fetal sterols. Because their heterozygous mothers synthesize cholesterol normally, virtually all sterols found in a Dhcr7 knockout fetus having a D...

journal_title：Journal of lipid research

authors： Tint GS,Yu H,Shang Q,Xu G,Patel SB

更新日期：2006-07-01 00:00:00

abstract：:The properties of cholesteryl oleate and triolein in mixed monolayers at the air-water interface have been measured between 24 and 37 degrees C. Analysis of force-area curves obtained as a function of the mol fraction of cholesteryl oleate indicates that at relatively low surface pressures these compounds are miscible...

journal_title：Journal of lipid research

authors： Smaby JM,Brockman HL

更新日期：1978-03-01 00:00:00

abstract：:Sixteen hyperlipidemic men were enrolled in a randomized, placebo-controlled, double-blind, cross-over study to evaluate the effect of ezetimibe 10 mg and simvastatin 40 mg, coadministered and alone, on the in vivo kinetics of apolipoprotein (apo) B-48 and B-100 in humans. Subjects underwent a primed-constant infusion...

journal_title：Journal of lipid research

authors： Tremblay AJ,Lamarche B,Hogue JC,Couture P

更新日期：2009-07-01 00:00:00

abstract：:We have reported the use of a retroviral vector to introduce the low density lipoprotein (LDL) receptor gene into receptor-deficient Watanabe heritable hyperlipidemic (WHHL) rabbit fibroblasts (Miyanohara, A., M. F. Sharkey, D. Steinberg, J. L. Witztum, and T. Friedmann. 1988. Proc. Natl. Acad. Sci. USA. 85: 6538-6542...

journal_title：Journal of lipid research

authors： Sharkey MF,Miyanohara A,Elam RL,Friedmann T,Witztum JL

更新日期：1990-12-01 00:00:00

abstract：:This study sought to clarify the contributions of organic anion-transporting polypeptide (OATP) 1B1 and 1B3 to the liver uptake of chenodeoxycholic acid (CDCA). We synthesized a fluorescent version of CDCA, chenodeoxychilyl-(Nepsilon-NBD)-lysine (CDCA-NBD), to characterize transporter-mediated uptake. CDCA-NBD is effi...

journal_title：Journal of lipid research

authors： Yamaguchi H,Okada M,Akitaya S,Ohara H,Mikkaichi T,Ishikawa H,Sato M,Matsuura M,Saga T,Unno M,Abe T,Mano N,Hishinuma T,Goto J

更新日期：2006-06-01 00:00:00

abstract：:The major net flux of cholesterol in the intact animal or human is from the peripheral organs to the liver. This flux is made up of cholesterol that is either synthesized in these peripheral tissues or taken up as lipoprotein cholesterol. This study investigates whether it is the concentration of apolipoprotein (apo) ...

journal_title：Journal of lipid research

authors： Jolley CD,Woollett LA,Turley SD,Dietschy JM

更新日期：1998-11-01 00:00:00

abstract：:The acute-phase response (APR) leads to alterations in lipid metabolism and type II nuclear hormone receptors, which regulate lipid metabolism, are suppressed, in liver, heart, and kidney. Here, we examine the effect of the APR in adipose tissue. In mice, lipopolysaccharide produces a rapid, marked decrease in mRNA le...

journal_title：Journal of lipid research

authors： Lu B,Moser AH,Shigenaga JK,Feingold KR,Grunfeld C

更新日期：2006-10-01 00:00:00

abstract：:Membranes of mammalian cells contain lysophosphatidic acid acyltransferase (LPAAT) activities that catalyze the acylation of sn-1-acyl lysophosphatidic acid (lysoPA) to form phosphatidic acid. As the biological roles and biochemical properties of the six known LPAAT isoforms have yet to be fully elucidated, we have ch...

journal_title：Journal of lipid research

authors： Hollenback D,Bonham L,Law L,Rossnagle E,Romero L,Carew H,Tompkins CK,Leung DW,Singer JW,White T

更新日期：2006-03-01 00:00:00

abstract：:Prostaglandin (PG)E(2) is relevant in tumor biology, and interactions between tumor and stroma cells dramatically influence tumor progression. We tested the hypothesis that cross-talk between head and neck squamous cell carcinoma (HNSCC) cells and fibroblasts could substantially enhance PGE(2) biosynthesis. We observe...

journal_title：Journal of lipid research

authors： Alcolea S,Antón R,Camacho M,Soler M,Alfranca A,Avilés-Jurado FX,Redondo JM,Quer M,León X,Vila L

更新日期：2012-04-01 00:00:00

abstract：:Gap junctions of rat brown adipose tissue (BAT) were studied with the freeze-fracture technique during development. The frequency and the mean area of gap junctions increased after birth, reached a maximal development during the functional phase of the tissue (birth to 3--4 weeks) and decreased while brown adipose tis...

journal_title：Journal of lipid research

authors： Schneider-Picard G,Carpentier JL,Orci L

更新日期：1980-07-01 00:00:00

abstract：:Mixed background SHP(-/-) mice are resistant to diet-induced obesity due to increased energy expenditure caused by enhanced PGC-1α expression in brown adipocytes. However, congenic SHP(-/-) mice on the C57BL/6 background showed normal expression of PGC-1α and other genes involved in brown adipose tissue thermogenesis....

journal_title：Journal of lipid research

authors： Park YJ,Kim SC,Kim J,Anakk S,Lee JM,Tseng HT,Yechoor V,Park J,Choi JS,Jang HC,Lee KU,Novak CM,Moore DD,Lee YK

更新日期：2011-12-01 00:00:00

abstract：:3-Hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase, the enzyme catalyzing the rate-limiting step in cholesterol biosynthesis, exists in one active (dephosphorylated) and one inactive (phosphorylated) form in liver microsomes obtained from several animal species. The present study was undertaken in order to dete...

journal_title：Journal of lipid research

authors： Angelin B,Einarsson K,Liljeqvist L,Nilsell K,Heller RA

更新日期：1984-11-01 00:00:00

abstract：:Previous studies have indicated that a G to A substitution at position -76 in the gene encoding apolipoprotein A-I (apoA-I) confers increased plasma high density lipoprotein cholesterol (HDL-C). Increased HDL-C may be a direct consequence of the A allele, or may reflect the action of a locus in linkage disequilibrium ...

journal_title：Journal of lipid research

authors： Barre DE,Guerra R,Verstraete R,Wang Z,Grundy SM,Cohen JC

更新日期：1994-07-01 00:00:00

abstract：:Lipids secreted form the bovine meibomian glands were assigned to following classes: cholesteryl esters (A) (fatty acyl chain lengths from 15 to 27 carbon atoms), 41%; wax esters, 29%; triacylglycerols, 10%; and cholesteryl ester (B) (fatty acyl chain lengths from 14 to 18 carbons), 15%. The remaining 5% consisted of ...

journal_title：Journal of lipid research

authors： Baron C,Blough HA

更新日期：1976-07-01 00:00:00