Gel permeation chromatography of neutral hydroxy lipids on Sephadex LH-20.

abstract：:A multiplexed assay was developed by MS to analyze, in a single run, six major human Apos involved in lipoprotein metabolism: ApoA-I, ApoA-II, ApoB100, ApoC-II, ApoC-III, and ApoE. This method was validated in vivo in six subjects who received a 14 h constant infusion of [5,5,5-(2)H3]L-leucine at 10 μM/kg/h. Plasma li...

journal_title：Journal of lipid research

authors： Croyal M,Fall F,Ferchaud-Roucher V,Chétiveaux M,Zaïr Y,Ouguerram K,Krempf M,Nobécourt E

更新日期：2016-03-01 00:00:00

abstract：:A gas-liquid chromatographic-mass spectrometric method of determining the position of oxygen atoms on polyfunctional fatty acids has been explored. The method consists of reduction of keto, hydroperoxy, epoxy, and carboxylic acid groups with LiAlH(4) to the corresponding alcohols; trimethylsilylation with bis(trimethy...

journal_title：Journal of lipid research

authors： Esselman WJ,Clagett CO

更新日期：1969-03-01 00:00:00

abstract：:Oxidized forms of cholesterol (oxysterols) are present in atherosclerotic lesions and may play an active role in lesion development. For example, 7-ketocholesterol (7KC) inhibits cholesterol efflux from macrophage foam cells induced by apolipoprotein A-I (apoA-I). Such oxysterols may promote foam cell formation in ath...

journal_title：Journal of lipid research

authors： Gelissen IC,Rye KA,Brown AJ,Dean RT,Jessup W

更新日期：1999-09-01 00:00:00

abstract：:ATP binding cassette transporter G1 (ABCG1) mediates the transport of cellular cholesterol to HDL, and it plays a key role in maintaining macrophage cholesterol homeostasis. During inflammation, HDL undergoes substantial remodeling, acquiring lipid changes and serum amyloid A (SAA) as a major apolipoprotein. In the cu...

journal_title：Journal of lipid research

authors： de Beer MC,Ji A,Jahangiri A,Vaughan AM,de Beer FC,van der Westhuyzen DR,Webb NR

更新日期：2011-02-01 00:00:00

abstract：:Lilly PCSK9 antibody LY3015014 (LY) is a monoclonal antibody (mAb) that neutralizes proprotein convertase subtilisin-kexin type 9 (PCSK9). LY decreases LDL cholesterol in monkeys and, unlike other PCSK9 mAbs, does not cause an accumulation of intact PCSK9 in serum. Comparing the epitope of LY with other clinically tes...

journal_title：Journal of lipid research

authors： Schroeder KM,Beyer TP,Hansen RJ,Han B,Pickard RT,Wroblewski VJ,Kowala MC,Eacho PI

更新日期：2015-11-01 00:00:00

abstract：:Sixteen hyperlipidemic men were enrolled in a randomized, placebo-controlled, double-blind, cross-over study to evaluate the effect of ezetimibe 10 mg and simvastatin 40 mg, coadministered and alone, on the in vivo kinetics of apolipoprotein (apo) B-48 and B-100 in humans. Subjects underwent a primed-constant infusion...

journal_title：Journal of lipid research

authors： Tremblay AJ,Lamarche B,Hogue JC,Couture P

更新日期：2009-07-01 00:00:00

abstract：:In a randomized, cross-over feeding trial involving 10 men with polygenic hypercholesterolemia, a control, Mediterranean-type cholesterol-lowering diet, and a diet of similar composition in which walnuts replaced approximately 35% of energy from unsaturated fat, were given for 6 weeks each. Compared with the control d...

journal_title：Journal of lipid research

authors： Muñoz S,Merlos M,Zambón D,Rodríguez C,Sabaté J,Ros E,Laguna JC

更新日期：2001-12-01 00:00:00

abstract：:In studies of cebus monkey plasma lipoproteins, we have used an ultracentrifugally generated density gradient to isolate two distinct species of low density lipoproteins (LDL). Compositional analyses revealed that each of the ultracentrifugally isolated fractions was enriched in cholesteryl esters and contained a sing...

journal_title：Journal of lipid research

authors： Puppione DL,Nicolosi RJ,Kowala MC,Schumaker VN

更新日期：1989-05-01 00:00:00

abstract：:Apolipoprotein A-V (apoA-V) first gained attention as a regulator of triglycerides through transgenic mouse studies. Furthermore, peroxisome proliferator-activated receptor alpha (PPARalpha) agonists such as fenofibrate increase apoA-V mRNA expression. Our group recently developed the first assay to quantitate serum a...

journal_title：Journal of lipid research

authors： Schultze AE,Alborn WE,Newton RK,Konrad RJ

更新日期：2005-08-01 00:00:00

abstract：:Recent findings described the role of CD36-mediated signaling in regulating cellular calcium and the release of various bioactive molecules, including the prostaglandins, neurotransmitters, cholecystokinin, and secretin. Here we document the role of CD36 in the secretion of hepatic VLDL. CD36 deletion resulted in 60% ...

journal_title：Journal of lipid research

authors： Nassir F,Adewole OL,Brunt EM,Abumrad NA

更新日期：2013-11-01 00:00:00

abstract：:The effect of inhibiting lysosomal protein degradation on the activity of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase was determined using a mouse mammary cell line (TS-85) which expresses a temperature-sensitive mutation in the ubiquitin degradative pathway. Incubating cells for 18 hr in medium containi...

journal_title：Journal of lipid research

authors： Tanaka RD,Li AC,Fogelman AM,Edwards PA

更新日期：1986-03-01 00:00:00

abstract：:The mRNA level for cytosolic NADP-dependent isocitrate dehydrogenase (IDH1) increases 2.3-fold, and enzyme activity of NADP-isocitrate dehydrogenase (IDH) 63%, in sterol-deprived HepG2 cells. The mRNA levels of the NADP- and NAD-dependent mitochondrial enzymes show limited or lack of regulation under the same conditio...

journal_title：Journal of lipid research

authors： Shechter I,Dai P,Huo L,Guan G

更新日期：2003-11-01 00:00:00

abstract：:Hepatic lipase (HL) is a lipolytic enzyme, synthesized by hepatocytes and found localized at the surface of liver sinusoid capillaries. In humans, the enzyme is mostly bound onto heparan-sulfate proteoglycans at the surface of hepatocytes and also of sinusoid endothelial cells. HL shares a number of functional domains...

journal_title：Journal of lipid research

authors： Perret B,Mabile L,Martinez L,Tercé F,Barbaras R,Collet X

更新日期：2002-08-01 00:00:00

abstract：:The common bile acids of rat bile (chenodeoxycholic, hyodeoxycholic, cholic, alpha-muricholic, and beta-muricholic acids) are completely separated by a new thin-layer chromatographic system. ...

journal_title：Journal of lipid research

authors： Siegfried CM,Elliott WH

更新日期：1968-05-01 00:00:00

abstract：:The rate-limiting step in the transit of dietary fat across the intestinal absorptive cell is its exit from the endoplasmic reticulum (ER) in a specialized ER-to-Golgi transport vesicle, the prechylomicron transport vesicle (PCTV). PCTV bud off from the ER membranes and have unique features; they are the largest ER-de...

journal_title：Journal of lipid research

authors： Siddiqi S,Siddiqi SA,Mansbach CM 2nd

更新日期：2010-05-01 00:00:00

abstract：:Trihydroxyoctadecenoic acids (TriHOMEs) are linoleic acid-derived oxylipins with potential physiological relevance in inflammatory processes as well as in maintaining an intact skin barrier. Due to the high number of possible TriHOME isomers with only subtle differences in their physicochemical properties, the stereoc...

journal_title：Journal of lipid research

authors： Fuchs D,Hamberg M,Sköld CM,Wheelock ÅM,Wheelock CE

更新日期：2018-10-01 00:00:00

abstract：:Seven Z-octadecenoic acids having the double bond located in positions 6Z to 13Z were photooxidized. The resulting hydroperoxy-E-octadecenoic acids [HpOME(E)] were resolved by chiral phase-HPLC-MS, and the absolute configurations of the enantiomers were determined by gas chromatographic analysis of diastereoisomeric d...

journal_title：Journal of lipid research

authors： Oliw EH,Wennman A,Hoffmann I,Garscha U,Hamberg M,Jernerén F

更新日期：2011-11-01 00:00:00

abstract：:The diffusion of [2-(14)C]glucose out of liposomes prepared from extracted human erythrocyte lipids was examined. Increased glucose efflux was observed when the lipids were treated with hydrogen peroxide and CuCl(2) before liposome formation, and this phenomenon required both peroxide and metal. Peroxidation of these ...

journal_title：Journal of lipid research

authors： Smolen JE,Shohet SB

更新日期：1974-05-01 00:00:00

abstract：:Lipid peroxidation is a common event in health and is greatly accelerated in pro-inflammatory settings such as hypercholesterolemia. Consequently, oxidation-specific epitopes are generated, which are pro-inflammatory and immunogenic, leading to both adaptive and innate responses. Because innate immune mechanisms use c...

journal_title：Journal of lipid research

authors： Hartvigsen K,Chou MY,Hansen LF,Shaw PX,Tsimikas S,Binder CJ,Witztum JL

更新日期：2009-04-01 00:00:00

abstract：:Humans and mice lacking angiopoietin-like protein 3 (ANGPTL3) have pan-hypolipidemia. ANGPTL3 inhibits two intravascular lipases, LPL and endothelial lipase, and the low plasma TG and HDL-cholesterol levels in ANGPTL3 deficiency reflect increased activity of these enzymes. The mechanism responsible for the low LDL-cho...

journal_title：Journal of lipid research

authors： Wang Y,Gusarova V,Banfi S,Gromada J,Cohen JC,Hobbs HH

更新日期：2015-07-01 00:00:00

abstract：:Three pentadecynoic acids, with the triple bond in the 8-, 9-, and 10-positions, have been synthesized on a gram scale in over-all yield of 65% by refinements of the five-step Ahmad-Strong method; isolation of intermediates was shown to be unnecessary prior to purification of the acetylenic nitriles by column chromato...

journal_title：Journal of lipid research

authors： Howton DR,Stein RA

更新日期：1969-11-01 00:00:00

abstract：:The 3-methyl-branched fatty acid phytanic acid is degraded by the peroxisomal alpha-oxidation route because the 3-methyl group blocks beta-oxidation. In adult Refsum disease (ARD), peroxisomal alpha-oxidation is defective, which is caused by mutations in the gene coding for phytanoyl-CoA hydroxylase in the majority of...

journal_title：Journal of lipid research

authors： Komen JC,Duran M,Wanders RJ

更新日期：2004-07-01 00:00:00

abstract：:The technique of freeze fracture was used to visualize triglyceride (TG) hydrolysis and the production of lipolytic products (LPs) in vitro and in vivo in the presence of bile salts (BS). Three systems were investigated: pure lipolytic products (oleic acid and monoolein) in the presence of a pure bile salt (taurodeoxy...

journal_title：Journal of lipid research

authors： Rigler MW,Honkanen RE,Patton JS

更新日期：1986-08-01 00:00:00

abstract：:Lipid concentrations are heritable traits. Recently, the number of known genetic loci associated with lipid levels in adults increased from 95 to 157. The effects of these 157 loci have not been tested in children. Considering that lipid levels track from childhood to adulthood, we studied to determine whether these v...

journal_title：Journal of lipid research

authors： Latsuzbaia A,Jaddoe VW,Hofman A,Franco OH,Felix JF

更新日期：2016-12-01 00:00:00

abstract：:Sebum is a complex lipid mixture that is synthesized in sebaceous glands and excreted on the skin surface. The purpose of this study was the comprehensive detection of the intact lipids that compose sebum. These lipids exist as a broad range of chemical structures and concentrations. Sebum was collected with SebuTape(...

journal_title：Journal of lipid research

authors： Camera E,Ludovici M,Galante M,Sinagra JL,Picardo M

更新日期：2010-11-01 00:00:00

abstract：:Conventional ideas concerning the unidirectional movement of triacylglycerol from intestinal lumen to lymph with sn-2-monoacylglycerol being the major glyceride-glycerol precursor were challenged by our finding that steady state specific activities of radiolabeled triacylglycerol (glyceryl moiety) in the intestinal mu...

journal_title：Journal of lipid research

authors： Mansbach CM 2nd,Parthasarathy S

更新日期：1982-09-01 00:00:00

abstract：:The polyene antibiotic amphotericin B (AMB) is an effective antifungal agent whose therapeutic potential is limited by poor aqueous solubility and toxicity toward host tissues. Addition of apolipoprotein A-I to a multilamellar phospholipid vesicle dispersion containing 20% (w/w) AMB induces the formation of reconstitu...

journal_title：Journal of lipid research

authors： Oda MN,Hargreaves PL,Beckstead JA,Redmond KA,van Antwerpen R,Ryan RO

更新日期：2006-02-01 00:00:00

abstract：:N-arachidonylethanolamine (AEA) accumulates during brain injury and postmortem. Because fatty acid amide hydrolase (FAAH) regulates brain AEA content, the purpose of this study was to determine its role in the postmortal accumulation of AEA using FAAH null mice. As expected, AEA content in immediately frozen brain tis...

journal_title：Journal of lipid research

authors： Patel S,Carrier EJ,Ho WS,Rademacher DJ,Cunningham S,Reddy DS,Falck JR,Cravatt BF,Hillard CJ

更新日期：2005-02-01 00:00:00

abstract：:High fatty acid (FA) flux is associated with systemic insulin resistance, and African-American (AA) women tend to be more insulin resistant. We assessed possible depot and race difference in the antilipolytic effect of insulin in adipocytes isolated from abdominal (Abd) and gluteal (Glt) subcutaneous (sc) adipose tiss...

journal_title：Journal of lipid research

authors： Fried SK,Tittelbach T,Blumenthal J,Sreenivasan U,Robey L,Yi J,Khan S,Hollender C,Ryan AS,Goldberg AP

更新日期：2010-05-01 00:00:00

abstract：:Glycolipids were isolated from the brain of a patient with a myoclonic variant of late infantile amaurotic idiocy. There was an abnormal glycolipid pattern in gray and white matter. The observed high concentration of gangliosides was due to a uniform accumulation of all four major gangliosides and was not limited to o...

journal_title：Journal of lipid research

authors： Bartsch GG

更新日期：1970-05-01 00:00:00