Abstract：
:The 3-methyl-branched fatty acid phytanic acid is degraded by the peroxisomal alpha-oxidation route because the 3-methyl group blocks beta-oxidation. In adult Refsum disease (ARD), peroxisomal alpha-oxidation is defective, which is caused by mutations in the gene coding for phytanoyl-CoA hydroxylase in the majority of ARD patients. As a consequence, phytanic acid accumulates in tissues and body fluids. This study focuses on an alternative route of phytanic acid degradation, omega-oxidation. The first step in omega-oxidation is hydroxylation at the omega-end of the fatty acid, catalyzed by a member of the cytochrome P450 multienzyme family. To study this first step, the formation of hydroxylated intermediates was studied in rat liver microsomes incubated with phytanic acid and NADPH. Two hydroxylated metabolites of phytanic acid were formed, omega- and (omega-1)-hydroxyphytanic acid (ratio of formation, 5:1). The formation of omega-hydroxyphytanic acid was NADPH dependent and inhibited by imidazole derivatives. These results indicate that phytanic acid undergoes omega-hydroxylation in rat liver microsomes and that an isoform of cytochrome P450 catalyzes the first step of phytanic acid omega-oxidation.
journal_name
J Lipid Resjournal_title
Journal of lipid researchauthors
Komen JC,Duran M,Wanders RJdoi
10.1194/jlr.M400064-JLR200keywords:
subject
Has Abstractpub_date
2004-07-01 00:00:00pages
1341-6issue
7eissn
0022-2275issn
1539-7262pii
M400064-JLR200journal_volume
45pub_type
杂志文章abstract：:Autotaxin (ATX), which is highly expressed and secreted by adipocytes, functions as the key enzyme to generate lysophosphatidic acid (LPA) from lysophosphatidylcholine. Adipose tissue is the main source of circulating ATX that modulates plasma LPA levels. Upregulation of ATX expression in obese patients and mice is cl...
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journal_title：Journal of lipid research
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journal_title：Journal of lipid research
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journal_title：Journal of lipid research
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journal_title：Journal of lipid research
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更新日期：2002-01-01 00:00:00
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journal_title：Journal of lipid research
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更新日期：1977-01-01 00:00:00
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journal_title：Journal of lipid research
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doi：
更新日期：1989-09-01 00:00:00
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journal_title：Journal of lipid research
pub_type： 杂志文章
doi：
更新日期：1983-02-01 00:00:00
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journal_title：Journal of lipid research
pub_type： 杂志文章
doi：10.1194/jlr.M200292-JLR200
更新日期：2003-02-01 00:00:00
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更新日期：2014-04-01 00:00:00
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pub_type： 杂志文章,随机对照试验
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更新日期：2011-10-01 00:00:00
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journal_title：Journal of lipid research
pub_type： 杂志文章
doi：
更新日期：1996-05-01 00:00:00
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journal_title：Journal of lipid research
pub_type： 杂志文章
doi：
更新日期：1969-03-01 00:00:00
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journal_title：Journal of lipid research
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doi：
更新日期：1991-10-01 00:00:00
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journal_title：Journal of lipid research
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doi：
更新日期：1992-12-01 00:00:00
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journal_title：Journal of lipid research
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更新日期：1993-05-01 00:00:00
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更新日期：1985-11-01 00:00:00
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更新日期：1971-05-01 00:00:00
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abstract：:Sebaceous glands are intriguing glands that are found throughout the human body except on the palms of the hands and soles of the feet. The true function of these glands has yet to be determined, but there are several theories, including antioxidant effects, antibacterial effects, and transport of pheromones. Sebaceou...
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更新日期：2008-02-01 00:00:00
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abstract：:The esterification of lysophospholipids contributes to phospholipid synthesis, remodeling, and scavenging. Acyl-CoA-dependent lysophospholipid acyltransferase activity with broad substrate use is mediated by Saccharomyces cerevisiae Lpt1p. We sought to identify Lpt1p active site amino acids besides the histidine conse...
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