Abstract:
:3alpha-Hydroxy-5-cholanoic acid (lithocholic acid) and some unidentified acids (one of them perhaps 3-hydroxy-cholest-5-en-26-oic acid) have been detected in the brains of guinea pigs afflicted with experimental "allergic" encephalomyelitis. None of the acids could be identified in comparable amounts of brain tissue from normal guinea pigs. Thin-layer chromatography of the free acids, and thin-layer and gas-liquid chromatography combined with mass spectrometry of the methyl esters were used for identification. The occurrence of these acids may possibly be the result of cholesterol oxidation in the brain. The acids, or compounds related to them, may play a role in development of demyelination in experimental allergic encephalomyelitis.
journal_name
J Lipid Resjournal_title
Journal of lipid researchauthors
Naqvi SH,Herndon BL,Kelley MT,Bleisch V,Aexel RT,Nicholas HJsubject
Has Abstractpub_date
1969-01-01 00:00:00pages
115-20issue
1eissn
0022-2275issn
1539-7262journal_volume
10pub_type
杂志文章abstract::This paper describes the purification and some of the properties of an enzyme from human spleen that catalyzes the hydrolysis of sphingomyelin with the formation of ceramide and phosphoryl choline. The enzyme, which is located in the subcellular particulate fraction that sediments between 700 and 8500 g, is readily ma...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1967-05-01 00:00:00
abstract::We studied the effects of the lecithin:cholesterol acyltransferase reaction on the size and composition of the small spherical high density lipoproteins of patients afflicted with familial lecithin:cholesterol acyltransferase deficiency. We isolated these lipoproteins by preparative ultracentrifugation and rate zonal ...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1984-03-01 00:00:00
abstract::Cytolipin R, a ceramide tetrahexoside isolated from rat lymphosarcoma, was studied by sequential hydrolysis with specific glycosidases which revealed the anomeric configurations of the glycosidic bonds. Sugar linkages were established by combined gas-liquid chromatography and mass spectrometry of the partially methyla...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1972-07-01 00:00:00
abstract::Ceramidases catalyze the cleavage of ceramides into sphingosine and fatty acids. Previously, we reported on the use of the RBM14 fluorogenic ceramide analogs to determine acidic ceramidase activity. In this work, we investigated the activity of other amidohydrolases on RBM14 compounds. Both bacterial and human purifie...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.D061564
更新日期:2015-10-01 00:00:00
abstract::Several studies have documented the presence of Lp[a] in nonhuman primates. However, data are lacking in great apes such as the chimpanzee. We have studied the quantitative distribution of Lp[a], as well as the frequency of apo[a] phenotypes, in a population of chimpanzees living in Gabon. Monoclonal antibody 14A12, d...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1994-02-01 00:00:00
abstract::Structurally specific very low density lipoprotein (VLDL) particles are presumed to redirect VLDL uptake during yolk deposition. Egg production is an energy intensive process, but energy excess negatively affects egg production. This study sought to determine whether overfeeding changed hen lipoprotein profiles, and i...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1994-08-01 00:00:00
abstract::Nonalcoholic fatty liver disease (NAFLD) is the most common liver disease in the United States and, indeed, worldwide. It has become a global public health issue. In the United States, the prevalence in the general population is estimated at approximately 20%, while that in the morbidly obese population at approximate...
journal_title:Journal of lipid research
pub_type: 杂志文章,评审
doi:10.1194/jlr.R800089-JLR200
更新日期:2009-04-01 00:00:00
abstract::In vivo single-voxel magnetic resonance spectroscopy (MRS) at 4.7T and ex vivo high-resolution proton magnetic resonance spectroscopy (HR-NMR) at 500 MHz were used to study the composition of adipose tissues in Zucker obese and Zucker lean rats. Lipid composition was characterized by unsaturation and polyunsaturation ...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M011825
更新日期:2011-02-01 00:00:00
abstract::Triglyceride-rich lipoproteins derived from ten normo- and hyperlipidemic apoE-2 homozygotes were analyzed for their composition, beta-VLDL content, and their ability to induce cholesteryl ester storage in macrophages. In six of these probands apoE sequence analysis revealed that the cysteine residues were at position...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1985-03-01 00:00:00
abstract::Pre-packed reverse phase columns (Bond Elut) were used for the separation of complex lipids, such as phosphatidylcholine, cerebrosides, sulfatides, and gangliosides, from their respective water-soluble radioactive precursors after their in vitro biosynthesis. After an incubation in vitro, the entire reaction mixture i...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1985-01-01 00:00:00
abstract::Recent findings described the role of CD36-mediated signaling in regulating cellular calcium and the release of various bioactive molecules, including the prostaglandins, neurotransmitters, cholecystokinin, and secretin. Here we document the role of CD36 in the secretion of hepatic VLDL. CD36 deletion resulted in 60% ...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M037812
更新日期:2013-11-01 00:00:00
abstract::An online, two-dimensional (2D) liquid chromatography (LC) quadrupole time-of-flight mass spectrometry (QToF-MS) method was developed for lipid profiling of rat peritoneal surface layers, in which the lipid classes and species could be simultaneously separated in one injection with a significantly increased sensitivit...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.D007567
更新日期:2010-09-01 00:00:00
abstract::Triglyceride (TG) concentration is used as a marker of cardiometabolic risk. However, diurnal and possibly weekday variation exists in TG concentrations. The objective of this work was to investigate weekday variation in TG concentrations among 1.8 million blood samples drawn between 2008 and 2015 from patients in the...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M074062
更新日期:2017-06-01 00:00:00
abstract::Protein farnesylation is a form of posttranslational modification that occurs in most, if not all, eukaryotic cells. Inhibitors of protein farnesyltransferase (PFTIs) have been developed as anticancer chemotherapeutic agents. Using the knowledge gained from the development of PFTIs for the treatment of cancer, researc...
journal_title:Journal of lipid research
pub_type: 杂志文章,评审
doi:10.1194/jlr.R500016-JLR200
更新日期:2006-02-01 00:00:00
abstract::Human high density lipoprotein enriched in free cholesterol was obtained by exposing the lipoprotein to lipid dispersions having a free cholesterol/lecithin molar ratio greater than two. The metabolism of cholesterol was studied in tissue culture cells exposed to normal and cholesterol-enriched lipoproteins. Incubatio...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1978-03-01 00:00:00
abstract::The diurnal cycle of hepatic HMG CoA reductase activity was studied under conditions of controlled feeding where the percentage of dietary lipid, alone or in combination with 2% cholestyramine, was varied. Cholestyramine caused an increase in HMG CoA reductase activity that began soon after feeding started and peaked ...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1972-11-01 00:00:00
abstract::Macrophages contain a neutral cholesteryl ester hydrolase that can be activated by cAMP-dependent protein kinase. Immunological studies strongly suggest that hormone-sensitive lipase (HSL) is probably responsible for the cholesteryl ester hydrolase activity in macrophages; however, due to the very low level of express...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1993-11-01 00:00:00
abstract::The mechanisms involved in the development of alcoholic liver disease (ALD) are not well established. We investigated the involvement of acyl-CoA: diacylglycerol acyltransferase 2 (DGAT2) upregulation in mediating hepatic fat accumulation induced by chronic alcohol consumption. Chronic alcohol feeding caused fatty liv...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M007948
更新日期:2010-11-01 00:00:00
abstract::The in vivo formation of the sulfate ester of glycolithocholate is a critical step in the elimination of this hepatotoxic bile salt. Rhesus monkeys fed chenodeoxycholate or ursodeoxycholate, the precursors of lithocholate, develop frank cirrhosis in association with accumulation of nonsulfated glycolithocholate in bil...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1986-11-01 00:00:00
abstract::Lipoproteins internalized by the LDL receptor (LDLR) are released from this receptor in endosomes through a process that involves acid-dependent conformational changes in the receptor ectodomain. How acidic pH promotes this release process is not well understood. Here, we assessed roles for six histidine residues for ...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M070938
更新日期:2017-02-01 00:00:00
abstract::Apolipoprotein A-II (apoA-II) is the second major apolipoprotein following apolipoprotein A-I (apoA-I) in HDL. ApoA-II has multiple physiological functions and can form senile amyloid fibrils (AApoAII) in mice. Most circulating apoA-II is present in lipoprotein A-I/A-II. To study the influence of apoA-I on apoA-II and...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M013235
更新日期:2011-08-01 00:00:00
abstract::Reduced white adipose tissue (WAT) LPL activity delays plasma clearance of TG-rich lipoproteins (TRLs). We reported the secretion of apoC-I, an LPL inhibitor, from WAT ex vivo in women. Therefore we hypothesized that WAT-secreted apoC-I associates with reduced WAT LPL activity and TRL clearance. WAT apoC-I secretion a...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.P064170
更新日期:2016-06-01 00:00:00
abstract::Two new 6-hydroxylated bile acids, 3 beta, 6 alpha, 12 alpha- and 3 beta, 6 beta, 12 alpha-trihydroxy-5 beta-cholanoic acids, were synthesized from deoxycholic acid. In addition, their C-3 epimers, 3 alpha, 6 alpha, 12 alpha- and 3 alpha, 6 beta, 12 alpha-trihydroxy acids, were prepared by a new route. The principal r...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1991-04-01 00:00:00
abstract::Electrospray ionization mass spectrometry has previously been used to probe qualitative changes in the phospholipid cardiolipin (CL), but it has rarely been used in a quantitative manner. We assessed changes in the amount of individual molecular species of cardiac CL present in a model of congestive heart failure usin...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M500031-JLR200
更新日期:2005-06-01 00:00:00
abstract::The transport of LDL-derived cholesterol from lysosomes to peroxisomes is facilitated by membrane contacts formed between the lysosomal protein synaptotagmin VII and the peroxisomal lipid phosphatidylinositol 4, 5-bisphosphate [PI(4,5)P2]. Here, we used RNA interference to search for regulators of PI(4,5)P2 and to stu...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M082149
更新日期:2018-03-01 00:00:00
abstract::A report on the effects of primary bile acid ingestion alone or in combination with plant sterols on serum cholesterol levels, biliary lipid secretion, and bile acid metabolism. Biliary bile acid and cholesterol secretion were measured in four patients with type IIa hypercholesterolemia before and after randomized tre...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1979-01-01 00:00:00
abstract::Apheresis is a treatment option for patients with severe hypercholesterolemia and coronary artery disease. It is, however, unknown whether such therapy changes kinetic parameters of lipoprotein metabolism, such as apolipoprotein B (apoB) secretion rates, conversion rates, and fractional catabolic rates (FCR). We studi...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1996-11-01 00:00:00
abstract::Acetyl-L-carnitine is known as a reservoir of activated acetyl units and as a modulator of metabolic function. The objective of this study was to quantify the fate of the acetyl moiety of acetyl-L-carnitine in lipogenic pathways. Lipogenesis was studied in an adipocyte model, differentiated 3T3-L1 cells, and a hepatom...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1997-07-01 00:00:00
abstract::Human endothelial lipase (EL), a member of the triglyceride lipase gene family, has five potential N-linked glycosylation sites, two of which are conserved in both lipoprotein lipase and hepatic lipase. Reduction in molecular mass of EL after treatment with glycosidases and after treatment of EL-expressing cells with ...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M400162-JLR200
更新日期:2004-11-01 00:00:00
abstract::Visceral fat has been associated with multiple cardiovascular disease (CVD) risk factors. The aim of this study was to identify anthropometrical measures most closely associated with some well-known CVD risk factors. Because most Asians at risk have normal body mass index (BMI) according to Western standards, we studi...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M300233-JLR200
更新日期:2003-12-01 00:00:00