Abstract:
:A reliable method for purifying envelope-stripped nuclei from immortalized murine embryonic fibroblasts (iMEFs) was established. Quantitative profiling of the glycerophospholipids (GPLs) in envelope-free iMEF nuclei yields several conclusions. First, we find the endonuclear glycerophospholipidome differs from that of bulk membranes, and phosphatidylcholine (PtdCho) and phosphatidylethanolamine species are the most abundant endonuclear GPLs by mass. By contrast, phosphatidylinositol (PtdIns) represents a minor species. We also find only a slight enrichment of saturated versus unsaturated GPL species in iMEF endonuclear fractions. Moreover, much lower values for GPL mass were measured in the iMEF nuclear matrix than those reported for envelope-stripped IMF-32 nuclei. The collective results indicate that the nuclear matrix in these cells is a GPL-poor environment where GPL occupies only approximately 0.1% of the total nuclear matrix volume. This value suggests GPL accommodation in this compartment can be satisfied by binding to resident proteins. Finally, we find no significant role for the PtdIns/PtdCho-transfer protein, PITPα, in shuttling PtdIns into the iMEF nuclear matrix.
journal_name
J Lipid Resjournal_title
Journal of lipid researchauthors
Tribble EK,Ivanova PT,Grabon A,Alb JG Jr,Faenza I,Cocco L,Brown HA,Bankaitis VAdoi
10.1194/jlr.M068734subject
Has Abstractpub_date
2016-08-01 00:00:00pages
1492-506issue
8eissn
0022-2275issn
1539-7262pii
jlr.M068734journal_volume
57pub_type
杂志文章abstract::Familial hypobetalipoproteinemia (FHBL) exists in three forms: a) FHBL genetically linked to truncated forms of apolipoprotein B (apoB); b) FHBL linked to the apoB gene but with no apoB truncations; and c) FHBL not linked to the apoB gene. Mean production rate (PR) of apoB-100 in FHBL subjects heterozygous for apoB tr...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1997-03-01 00:00:00
abstract::Elongation of very long chain fatty acids (ELOVL)5 is one of seven mammalian fatty acid condensing enzymes involved in microsomal fatty acid elongation. To determine the in vivo substrates and function of ELOVL5, we generated Elovl5(-/-) mice. Studies using liver microsomal protein from wild-type and knockout mice dem...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M800383-JLR200
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abstract::A synthetic analog of glucocerebroside, N-hexyl-O-glucosyl sphingosine, was found to inhibit the glucosidase in rat spleen that hydrolyzes glucocerebroside. At a concentration of 1 micro m, the analog inhibited the enzyme by 48%. The mode of action appeared to be competitive, probably aided by tight binding of the ami...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1973-03-01 00:00:00
abstract::Serum paraoxonase (PON1) is a HDL-associated enzyme exhibiting potentially antiatherogenic properties. Here, we examined the common PON1-192R/Q human polymorphism. Despite numerous studies, the effect of this polymorphism on the antiatherogenic potential of PON1 is yet unresolved. Our structural model suggests that am...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M600297-JLR200
更新日期:2006-11-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1998-12-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1993-08-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M800364-JLR200
更新日期:2008-12-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1969-07-01 00:00:00
abstract::Retinoic acid (RA), the active metabolite of vitamin A, is required for spermatogenesis and many other biological processes. RA formation requires irreversible oxidation of retinal to RA by aldehyde dehydrogenase enzymes of the 1A family (ALDH1A). While ALDH1A1, ALDH1A2, and ALDH1A3 all form RA, the expression pattern...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M054718
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1986-06-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1982-03-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1988-10-01 00:00:00
abstract::Serum amyloid A (SAA) is an acute phase reactant that can become the predominant apolipoprotein of high density lipoprotein (HDL) during severe inflammatory states. However, the function of SAA is unknown. To study the ability of SAA to form HDL in the absence of apolipoprotein A-I, we expressed the mouse SAA pI 6.15 ...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1997-08-01 00:00:00
abstract::Abetalipoproteinemia (ABL) and chylomicron retention disease (CMRD) are extremely rare recessive forms of hypobetalipoproteinemia characterized by intestinal lipid malabsorption and severe vitamin E deficiency. Vitamin E is often supplemented in the form of fat-soluble vitamin E acetate, but fat malabsorption consider...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M085043
更新日期:2018-09-01 00:00:00
abstract::Cholesterol efflux (CE) capacity has been inversely associated with atherosclerosis and may provide an insight on inflammation occurring in human immunodeficiency virus (HIV) individuals. We address this by studying CE in HIV patients at different stages of HIV disease progression. In this cross-sectional study, CE fr...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M088153
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abstract::Insulin resistance (IR) is associated with elevated plasma levels of triglyceride-rich lipoproteins (TRLs) of intestinal origin. However, the mechanisms underlying the overaccumulation of apolipoprotein (apo)B-48-containing TRLs in individuals with IR are not yet fully understood. This study examined the relationships...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M040071
更新日期:2014-01-01 00:00:00
abstract::Dietary n-3 long-chain PUFAs (LC-PUFAs) are associated with improvement in the parameters of the metabolic syndrome (MetS). Glucokinase regulatory protein (GCKR) is a key protein regulating intracellular glucose disposal. Our aim was to investigate: i) the relationship between the GCKR rs1260326 (Pro446Leu) polymorphi...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M057570
更新日期:2015-09-01 00:00:00
abstract::An enzyme-coupled, colorimetric method for the assay of acyl-CoA synthetase is described. Acyl-CoA formed from fatty acid and CoA by acyl-CoA synthetase was dehydrogenated by acyl-CoA oxidase. Hydrogen peroxide produced was then converted into formaldehyde in the presence of methanol by catalase. The formaldehyde reac...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1991-10-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1993-09-01 00:00:00
abstract::We investigated the role of caveolae in uptake and intracellular trafficking of long chain fatty acids (LCFA) in HepG2 human hepatoma cells. The uptake of [(3)H]oleic acid and [(3)H]stearic acid into HepG2 cells was measured by radioactive assays and internalization of the non-metabolizable fluorescent fatty acid 12-(...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.m100404-jlr200
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abstract::We have studied the synthesis, modification, and secretion of rat apoE in primary cultures of neonatal aortic smooth muscle cells and adult rat hepatocytes. The cultures were pulsed with [35S]methionine and the intracellular and secreted apoE were immunoprecipitated and analyzed by two-dimensional isoelectric focusing...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1988-07-01 00:00:00
abstract::Mammalian spermatogenesis is a complex developmental program in which a diploid progenitor germ cell transforms into highly specialized spermatozoa. One intriguing aspect of sperm production is the dynamic change in membrane lipid composition that occurs throughout spermatogenesis. Cholesterol content, as well as its ...
journal_title:Journal of lipid research
pub_type: 杂志文章,评审
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更新日期:2013-01-01 00:00:00
abstract::Sulfoquinovosyl diglyceride from plant tissues is recovered in sizeable quantities, free from phospholipids and galactolipids. ...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1966-07-01 00:00:00
abstract::In late-stage atherosclerosis, much of the cholesterol in macrophage foam cells resides within enlarged lysosomes. Similarly, human macrophages incubated in vitro with modified LDLs contain significant amounts of lysosomal free cholesterol and cholesteryl ester (CE), which disrupts lysosomal function similar to macrop...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M800659-JLR200
更新日期:2009-10-01 00:00:00
abstract::Selectivity of CDP-choline:diacylglycerol choline phosphotransferase and CDP-ethanolamine:diacylglycerol ethanolamine phosphotransferase for molecular species of diglyceride has been studied in rat brain microsomes in vitro. Diglyceride-labeled microsomes were prepared by incubation with labeled sn-glycerol-3-phosphat...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1980-05-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M050286
更新日期:2014-11-01 00:00:00
abstract::The transport of LDL-derived cholesterol from lysosomes to peroxisomes is facilitated by membrane contacts formed between the lysosomal protein synaptotagmin VII and the peroxisomal lipid phosphatidylinositol 4, 5-bisphosphate [PI(4,5)P2]. Here, we used RNA interference to search for regulators of PI(4,5)P2 and to stu...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M082149
更新日期:2018-03-01 00:00:00
abstract::The purpose of the present study was to examine the regulation of levels of apolipoprotein B (apoB) mRNA and its protein by cytokines in HepG2 cells. A dose-dependent increase in apoB mRNA levels was observed in the presence of either interleukin-1beta (IL-1beta) or IL-6 alone. This increase occurred as early as 1 h a...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1998-01-01 00:00:00
abstract::Glycerolipid and apoB synthesis and secretion were examined in hepatocytes obtained from fetal, suckling (day 6), and adult rats in order to examine the developmental regulation of lipoprotein production. The capacity to synthesize [3H]triacylglycerol (from [3H]glycerol) followed the order: adult greater than day 6 gr...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1988-01-01 00:00:00
abstract::Studies on the effects of longitudinal lipid trajectories on end-stage renal disease (ESRD) development and deaths among patients with chronic kidney disease (CKD) are limited. We conducted a registry-based prospective study using data from a 13-year multidisciplinary pre-ESRD care program. The final study population ...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.P084590
更新日期:2019-03-01 00:00:00