Solid-phase peptide synthesis and solid-state NMR spectroscopy of [Ala3-15N][Val1]gramicidin A.

abstract：:Classical studies suggest that high-level cognitive decisions (e.g., choosing between financial options) are suboptimal. In contrast, low-level decisions (e.g., choosing where to put your feet on a rocky ridge) appear near-optimal: the perception-cognition gap. Moreover, in classical tasks, people appear to put too mu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jarvstad A,Hahn U,Rushton SK,Warren PA

更新日期：2013-10-01 00:00:00

abstract：:Evolution of an oxygenic atmosphere required primordial life to accommodate the toxicity associated with reactive oxygen species. We have characterized an archaeal antioxidant from the hyperthermophilic acidophile Sulfolobus solfataricus. The amino acid sequence of this approximately 22-kDa protein shares little seque...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wiedenheft B,Mosolf J,Willits D,Yeager M,Dryden KA,Young M,Douglas T

更新日期：2005-07-26 00:00:00

abstract：:We report the cloning and expression of a cDNA encoding human sterol carrier protein 2 (SCP2). The 1.3-kilobase (kb) cDNA contains an open reading frame which encompasses a 143-amino acid sequence which is 89% identical to the rat SCP2 amino acid sequence. The deduced amino acid sequence of the polypeptide reveals a 2...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yamamoto R,Kallen CB,Babalola GO,Rennert H,Billheimer JT,Strauss JF 3rd

更新日期：1991-01-15 00:00:00

abstract：:This paper shows that the most predictable components of internal variability in coupled atmosphere-ocean models are remarkably similar to the most predictable components of climate models without interactive ocean dynamics (i.e., models whose ocean is represented by a 50-m-deep slab ocean mixed layer with no interact...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Srivastava A,DelSole T

更新日期：2017-02-28 00:00:00

abstract：:Cytogenetic analysis of acute myeloid leukemia (AML) cells has accelerated the identification of genes important for AML pathogenesis. To complement cytogenetic studies and to identify genes altered in AML genomes, we performed genome-wide copy number analysis with paired normal and tumor DNA obtained from 86 adult pa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Walter MJ,Payton JE,Ries RE,Shannon WD,Deshmukh H,Zhao Y,Baty J,Heath S,Westervelt P,Watson MA,Tomasson MH,Nagarajan R,O'Gara BP,Bloomfield CD,Mrózek K,Selzer RR,Richmond TA,Kitzman J,Geoghegan J,Eis PS,Maupin R,

更新日期：2009-08-04 00:00:00

abstract：:The temporal and spatial patterns of floral determination in cultured internode segments of Nicotiana tabacum L. cv. Wisconsin 38 were investigated. Segments from internodes, when cultured on hormone-free medium, either produced some de novo floral shoots or produced only vegetative shoots. Segments that produced some...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Singer SR,McDaniel CN

更新日期：1987-05-01 00:00:00

abstract：:Light microscopy autoradiography has been used to visualize neuroanatomical patterns of brain opiate receptor upregulation in response to chronic naltrexone administration. Slide-mounted brain sections of frozen rat brain were labeled in vitro with dihydro[3H]morphine, a relatively selective mu opioid ligand. The grea...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tempel A,Gardner EL,Zukin RS

更新日期：1984-06-01 00:00:00

abstract：:A new class of Escherichia coli mutants, referred to as grn, has been isolated by localized mutagenesis. These mutations affect the sigma subunit of DNA-dependent RNA polymerase (ribonucleoside 5'-triphosphate:RNA nucleotidyltransferase, EC 2.7.7.6) by abolishing the expression of the lambda N gene, and they are close...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nakamura Y,Kurihara T,Saito H,Uchida H

更新日期：1979-09-01 00:00:00

abstract：:Nuclear structural changes during fertilization and embryogenesis in mice and in sea urchins have been followed by using antibodies against the nuclear lamins A/C and B and against antigens at the periphery of nuclei and chromosomes. Lamins are found on all pronuclei and nuclei during mouse fertilization, but with a d...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schatten G,Maul GG,Schatten H,Chaly N,Simerly C,Balczon R,Brown DL

更新日期：1985-07-01 00:00:00

abstract：:Cytochrome P450(BioI) (CYP107H1) from the biotin operon of Bacillus subtilis forms a seven-carbon diacid through a multistep oxidative cleavage of a fatty acid linked to acyl carrier protein (ACP). Crystal structures of P450(BioI) in complex with three different length fatty acyl-ACP (Escherichia coli) ligands show th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cryle MJ,Schlichting I

更新日期：2008-10-14 00:00:00

abstract：:Analysis of the nucleotide and deduced amino acid sequences of the biologically active mouse mdr1 cDNA clone indicates that the protein is formed by two highly homologous halves, each containing six putative transmembrane domains and a nucleotide-binding site. The duplicated unit shows high sequence homology to the pr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Raymond M,Gros P

更新日期：1989-09-01 00:00:00

abstract：:Astrocytes can release glutamate in a calcium-dependent manner and consequently signal to adjacent neurons. Whether this glutamate release pathway is used during physiological signaling or is recruited only under pathophysiological conditions is not well defined. One reason for this lack of understanding is the limite...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Parpura V,Haydon PG

更新日期：2000-07-18 00:00:00

abstract：:Variation between North Korean and Latin American isolates in the circumsporozoite (CS) protein encoding gene of the human malaria parasite Plasmodium vivax was studied. Polymorphic positions are confined to the central tandemly repeated sequences. Nucleotide substitutions in the tandem repeats produce variants; these...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Arnot DE,Barnwell JW,Stewart MJ

更新日期：1988-11-01 00:00:00

abstract：:A simple method combining photolithography and shadow (or angle) evaporation is developed to fabricate single-walled carbon nanotube (SWCNT) devices with tube lengths of approximately 10-50 nm between metal contacts. Large numbers of such short devices are obtained without the need of complex tools such as electron be...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Javey A,Qi P,Wang Q,Dai H

更新日期：2004-09-14 00:00:00

abstract：:A full-length cDNA for tryptophan hydroxylase was cloned from rabbit pineal body by screening an expression library with antibody against rat phenylalanine hydroxylase, which crossreacts with rabbit tryptophan hydroxylase. Clones producing immunoreactive material contain sequences homologous to, yet distinct from, phe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Grenett HE,Ledley FD,Reed LL,Woo SL

更新日期：1987-08-01 00:00:00

abstract：:We have developed a method for conjugating low density lipoproteins (LDL) with colloidal gold. Conjugation, complete after 1 min, occurs by electrostatic adsorption of the LDL to the negatively charged gold particle. Each conjugate consists of approximately eight biologically active LDL molecules clustered around a ce...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Handley DA,Arbeeny CM,Witte LD,Chien S

更新日期：1981-01-01 00:00:00

abstract：:Aquaporin (AQP) 4 is the predominant water channel in the mammalian brain, abundantly expressed in the blood-brain and brain-cerebrospinal fluid interfaces of glial cells. Its function in cerebral water balance has implications in neuropathological disorders, including brain edema, stroke, and head injuries. The 1.8-A...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ho JD,Yeh R,Sandstrom A,Chorny I,Harries WE,Robbins RA,Miercke LJ,Stroud RM

更新日期：2009-05-05 00:00:00

abstract：:A hierarchy of residue density assessments and packing properties in protein structures are contrasted, including a regular density, a variety of charge densities, a hydrophobic density, a polar density, and an aromatic density. These densities are investigated by alternative distance measures and also at the interfac...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Baud F,Karlin S

更新日期：1999-10-26 00:00:00

abstract：:l-Serine biosynthesis, a crucial metabolic process in most domains of life, is initiated by d-3-phosphoglycerate (d-3-PG) dehydrogenation, a thermodynamically unfavorable reaction catalyzed by d-3-PG dehydrogenase (SerA). d-2-Hydroxyglutarate (d-2-HG) is traditionally viewed as an abnormal metabolite associated with c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhang W,Zhang M,Gao C,Zhang Y,Ge Y,Guo S,Guo X,Zhou Z,Liu Q,Zhang Y,Ma C,Tao F,Xu P

更新日期：2017-09-05 00:00:00

abstract：:The BAG6 complex was first identified as an upstream loading factor for tail-anchored membrane proteins entering the TRC40-dependent pathway for posttranslational delivery to the endoplasmic reticulum. Subsequently, BAG6 was shown to enhance the proteasomal degradation of mislocalized proteins by selectively promoting...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Leznicki P,High S

更新日期：2012-11-20 00:00:00

abstract：:We have screened a large number of isolated human genomic clones that hybridize to a cloned HLA cDNA probe for their ability to direct the synthesis of HLA-A, -B, and -C surface antigens on mouse L cells following DNA-mediated gene transfer. The surface expression of human histocompatibility antigens, monitored by ind...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Barbosa JA,Kamarck ME,Biro PA,Weissman SM,Ruddle FH

更新日期：1982-10-01 00:00:00

abstract：:Transporters in the Golgi apparatus membrane translocate nucleotide sugars from the cytosol into the Golgi lumen before these can be substrates for the glycosylation of proteins, lipids, and proteoglycans. We have cloned the mammalian Golgi membrane transporter for uridine diphosphate-N-acetylglucosamine by phenotypic...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Guillen E,Abeijon C,Hirschberg CB

更新日期：1998-07-07 00:00:00

abstract：:Muscle wasting is a debilitating consequence of fasting, inactivity, cancer, and other systemic diseases that results primarily from accelerated protein degradation by the ubiquitin-proteasome pathway. To identify key factors in this process, we have used cDNA microarrays to compare normal and atrophying muscles and f...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gomes MD,Lecker SH,Jagoe RT,Navon A,Goldberg AL

更新日期：2001-12-04 00:00:00

abstract：:The effect of the IgG from amyotrophic lateral sclerosis (ALS) patients was tested on the voltage-dependent barium currents (IBa) in mammalian dissociated Purkinje cells and in isolated P-type calcium channels in lipid bilayers. Whole cell clamp of Purkinje cells demonstrates that ALS IgG increases the amplitude of IB...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Llinás R,Sugimori M,Cherksey BD,Smith RG,Delbono O,Stefani E,Appel S

更新日期：1993-12-15 00:00:00

abstract：:P/Q Ca(2+) channel activity is inhibited by G protein-coupled receptor activation. Channel inhibition requires a direct Gbetagamma binding onto the pore-forming subunit, Ca(v)2.1. It is characterized by biophysical changes, including current amplitude reduction, activation kinetic slowing, and an I-V curve shift, whic...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sandoz G,Lopez-Gonzalez I,Grunwald D,Bichet D,Altafaj X,Weiss N,Ronjat M,Dupuis A,De Waard M

更新日期：2004-04-20 00:00:00

abstract：:Molybdate- and ATP-dependent in vitro synthesis of the iron-molybdenum cofactor (FeMo-co) of nitrogenase requires the protein products of at least the nifB, nifN, and nifE genes. Extracts of FeMo-co-negative mutants of Klebsiella pneumoniae and Azotobacter vinelandii with lesions in different genes can be complemented...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shah VK,Imperial J,Ugalde RA,Ludden PW,Brill WJ

更新日期：1986-03-01 00:00:00

abstract：:The motor protein cytoplasmic dynein is responsible for most of the minus-end-directed microtubule traffic within cells. Dynein contains four evolutionarily conserved AAA (ATPase associated with various cellular activities) domains that are thought to bind nucleotide; the role of nucleotide binding and hydrolysis in e...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Reck-Peterson SL,Vale RD

更新日期：2004-02-10 00:00:00

abstract：:A human beta-globin gene derived from an established human lymphoblast cell line was introduced into murine erythroleukemia (MEL) cells by cell fusion. The globin genes in MEL cells are inducible by dimethyl sulfoxide (Me2SO); induction leads to the accumulation of mouse globin mRNA and hemoglobin. Globin mRNA was not...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pyati J,Kucherlapati RS,Skoultchi AI

更新日期：1980-06-01 00:00:00

abstract：:At least two kidney epithelial cell lines, the Madin-Darby canine kidney (MDCK) and the murine inner medullary collecting duct line mIMCD-3, can be induced to form branching tubular structures when cultured with hepatocyte growth factor (HGF) plus serum in collagen I gels. In our studies, whereas MDCK cells remained u...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Barros EJ,Santos OF,Matsumoto K,Nakamura T,Nigam SK

更新日期：1995-05-09 00:00:00

abstract：:We report that diverse species of bacteria encode a type IB DNA topoisomerase that resembles vaccinia virus topoisomerase. Deinococcus radiodurans topoisomerase IB (DraTopIB), an exemplary member of this family, relaxes supercoiled DNA in the absence of a divalent cation or ATP. DraTopIB has a compact size (346 aa) an...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Krogh BO,Shuman S

更新日期：2002-02-19 00:00:00