Abstract:
:Spirochetes have been implicated as potential etiologic agents of periodontitis in humans. Murine monoclonal antibodies (MAbs) specific for a serogroup of Treponema denticola, an oral spirochete, were developed and characterized in this study. Antibodies secreted by clone IAA11 were judged to be the most useful, since they were able to detect 8 of 15 T. denticola strains. This MAb consisted of an immunoglobulin G3 heavy chain and a kappa light chain. MAb IAA11 was found to react with an epitope target located on the outer sheath of the cell wall. This MAb should be of diagnostic and scientific value in the study of T. denticola populations in human periodontitis.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Simonson LG,Rouse RF,Bockowski SWdoi
10.1128/IAI.56.1.60-63.1988subject
Has Abstractpub_date
1988-01-01 00:00:00pages
60-3issue
1eissn
0019-9567issn
1098-5522journal_volume
56pub_type
杂志文章abstract::In an earlier report, we had described the isolation and characterization of autolysis-defective mutants of Staphylococcus aureus (N. Mani, P. Tobin, and R.K. Jayaswal, J. Bacteriol. 175:1493-1499, 1993). In the study reported here, an autolysis-defective mutant showed attenuated virulence in a rat model of experiment...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.4.1406-1409.1994
更新日期:1994-04-01 00:00:00
abstract::Controversy persists over the role that the capsular polysaccharide plays in the pathogenesis of Staphylococcus aureus infections. To address this issue, we compared the mouse virulence of S. aureus Reynolds and capsule-defective mutant strains cultivated under conditions of high or low capsule expression. Strain Reyn...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.11.5183-5189.1998
更新日期:1998-11-01 00:00:00
abstract::Toxin preparations were obtained by growing Clostridium difficile VPI strain 10463 in 2-liter brain heart infusion dialysis flasks at 37 degrees C for 3 days. The initial step of the purification scheme involved ultrafiltration through an XM-100 membrane filter. Two toxic activities, designated toxins A and B, were se...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.35.3.1032-1040.1982
更新日期:1982-03-01 00:00:00
abstract::Because of the association of the group A streptococcal pyrogenic exotoxins (SPEs) with erythrogenic toxin used in the classical Dick test, the involvement of the SPEs in production of erythematous skin reactions was assessed. Unless they had been presensitized, young adult rabbits failed to show skin reactions after ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.26.2.467-472.1979
更新日期:1979-11-01 00:00:00
abstract::In order to deploy virulence factors at appropriate times and locations, microbes must rapidly sense and respond to various metabolite signals. Previously, we showed a transient elevation of the methionine-derived metabolite methylthioadenosine (MTA) concentration in serum during systemic Salmonella enterica serovar T...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00429-18
更新日期:2018-08-22 00:00:00
abstract::A T antigen preparation free of trypsin was obtained by application of CNBr-Sepharose linked to pure trypsin. Purification on an ion exchange chromatography column results in an electrophoretically homogeneous preparation of T protein. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.13.3.993-994.1976
更新日期:1976-03-01 00:00:00
abstract::Lower genital tract infection with Chlamydia trachomatis and C. muridarum can induce long-lasting hydrosalpinx in the upper genital tract of women and female mice, respectively. However, A/J mice were highly resistant to induction of long-lasting hydrosalpinx by C. muridarum. We further compared host inflammatory resp...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00055-14
更新日期:2014-07-01 00:00:00
abstract::A system is described for studying adoptive immunity to tuberculosis in syngeneic mice. Donor mice were immunized with 10(4) BCG intravenously, and lymphoid cells were harvested 28 days later. Adoptive immunity was measured in recipient mice in terms of the inhibition of growth of BCG in the liver and spleen following...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.11.6.1174-1181.1975
更新日期:1975-06-01 00:00:00
abstract::The serum antibody response and four different cellular immune responses (cytotoxic T cells, delayed-type hypersensitivity T cells, natural killer cells, and cytotoxic macrophage levels) induced in CBA/H mice were measured at different times after intranasal inoculation of a cold-adapted (ca) variant of influenza A vi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.38.1.218-225.1982
更新日期:1982-10-01 00:00:00
abstract::Porphyromonas gingivalis has been implicated as an etiologic agent of adult periodontitis. We have previously shown that P. gingivalis can degrade the epithelial cell-cell junction complexes, thus suggesting that this bacterium can invade the underlying connective tissues via a paracellular pathway. However, the preci...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.5.2512-2518.2002
更新日期:2002-05-01 00:00:00
abstract::Streptococcal protective antigen (Spa) is a newly described surface protein of group A streptococci that was recently shown to evoke protective antibodies (J. B. Dale, E. Y. Chiang, S. Liu, H. S. Courtney, and D. L. Hasty, J. Clin. Investig. 103:1261--1268, 1999). In this study, we have determined the complete sequenc...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.5.2943-2949.2001
更新日期:2001-05-01 00:00:00
abstract::Shigella flexneri is a gram-negative bacterium that causes bacillary dysentery in humans that is characterized by an acute inflammatory response of the colon. The fate of phagocytes that are infected in vitro with virulent Shigella has been the subject of some investigation and debate. In this study we found that viru...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.1.504-513.2005
更新日期:2005-01-01 00:00:00
abstract::Lipoteichoic acids (LTAs) belong to the immunostimulatory class of molecules of gram-positive bacteria (GPB). Previous investigations showed that the macrophage scavenger receptor (SR), a glycosylated trimeric transmembrane protein, binds directly to many GPB, possibly via LTA. SR binding to other ligands is dependent...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.8.3318-3325.1996
更新日期:1996-08-01 00:00:00
abstract::The yeast form of the dimorphic fungus Histoplasma capsulatum survives within macrophages after phagocytosis. To do so, it must avoid, inhibit, or resist a variety of toxic oxygen metabolites. Using ferricytochrome c reduction to assay superoxide release, we examined the response of mouse macrophages to the yeast form...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.1.29-34.1987
更新日期:1987-01-01 00:00:00
abstract::The human gastric pathogen Helicobacter pylori modifies host cholesterol via glycosylation and incorporates the glycosylated cholesterol into its membrane; however, the benefits of cholesterol to H. pylori are largely unknown. We speculated that cholesterol in the H. pylori membrane might alter the susceptibility of t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00974-09
更新日期:2011-01-01 00:00:00
abstract::Ehrlichia chaffeensis, a tick-transmitted obligate intracellular rickettsial agent, causes human monocytic ehrlichiosis. In recent reports, we described substantial advances in developing random and targeted gene disruption methods to investigate the functions of E. chaffeensis genes. We reported earlier that the Hima...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00316-20
更新日期:2020-09-18 00:00:00
abstract::Type III and type VI secretion systems (T3SSs and T6SSs, respectively) are critical virulence determinants in several Gram-negative pathogens. In Burkholderia pseudomallei, the T3SS-3 and T6SS-1 clusters have been implicated in bacterial virulence in mammalian hosts. We recently discovered a regulatory cascade that co...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.05148-11
更新日期:2011-08-01 00:00:00
abstract::Vaccine antigen-specific antibody-secreting cell (ASC) responses in peripheral blood of healthy adult volunteers were studied after oral immunization with a prototype enterotoxigenic Escherichia coli (ETEC) vaccine by means of the enzyme-linked immunospot technique. Three doses of vaccine consisting of formalin-killed...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.7.2605-2611.1992
更新日期:1992-07-01 00:00:00
abstract::Knowledge of whether Mycobacterium tuberculosis resides within a relatively impermeable membrane-bound vacuole or is free within the cytoplasm within its host cell is central to an understanding of the immunobiology of this intracellular parasite but is a matter of controversy. To explore this issue, we assessed the a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.10.5800-5807.2002
更新日期:2002-10-01 00:00:00
abstract::Chagas' disease is a chronic infection caused by Trypanosoma cruzi and represents an important public health burden in Latin America. Frequently the disease evolves undetectable for decades, while in a significant fraction of the affected individuals it culminates in death by heart failure. Here, we describe a novel m...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.72.4.2350-2357.2004
更新日期:2004-04-01 00:00:00
abstract::Several monoclonal antibodies directed against gonococcal pili have been used to investigate the potential contribution of anti-pilus antibodies to host defense against gonococcal infection. Included were two antibodies (SM1 and SM2) which reacted with conserved determinants present on pili from all strains tested and...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.49.3.621-628.1985
更新日期:1985-09-01 00:00:00
abstract::The Legionella pneumophila major secretory protein (Msp) is a Zn2+ metalloprotease whose function in pathogenesis is unknown. The structural gene for the Msp protease, mspA, was isolated from an L. pneumophila genomic library. In Escherichia coli which contain plasmids with the mspA gene, Msp protein and activity are ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.8.2585-2592.1990
更新日期:1990-08-01 00:00:00
abstract::The possibility that gonococcal energy might play a role in the interaction of Neisseria gonorrhoeae with both phagocytic and nonphagocytic cells was examined. Respiratory chain inhibitors including KCN and amobarbital resulted in reduction in gonococcal association with human neutrophils. Similar results were seen wi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.3.785-790.1989
更新日期:1989-03-01 00:00:00
abstract::Hamster respiratory epithelial cells were cultured in a monolayer format, and 20% of the cells were ciliated. Mycoplasma pneumoniae attached to the epithelial cells in a neuraminidase-specific fashion and induced ciliostasis and cytonecrosis. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.21.1.333-336.1978
更新日期:1978-07-01 00:00:00
abstract::Endotoxin-treated mice exhibit a rapid rise in the level of the serum amyloid A (SAA) protein, but this effect is not observed in endotoxin-resistant C3H/HeJ mice. To evaluate the role of lymphoid cells in the production of SAA protein, C3H/HeJ mice were adoptively transfused with endotoxin-sensitive (C3H/HeN) bone ma...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.23.1.181-183.1979
更新日期:1979-01-01 00:00:00
abstract::Bordetella bronchiseptica secreted a hydroxamate siderophore when grown in Fe-depleted medium. A Tn5lac insertion mutant of B. bronchiseptica, DBB22, did not produce this hydroxamate siderophore and was incapable of using lactoferrin as an Fe source. Our data suggest that B. bronchiseptica uses a siderophore for remov...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.6.2698-2702.1993
更新日期:1993-06-01 00:00:00
abstract::Haemophilus ducreyi, the etiologic agent of the sexually transmitted disease chancroid, has been shown to inhibit phagocytosis of both itself and secondary targets in vitro. Immunodepletion of LspA proteins from H. ducreyi culture supernatant fluid abolished this inhibitory effect, indicating that the LspA proteins ar...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.12.7808-7816.2005
更新日期:2005-12-01 00:00:00
abstract::Neisseria meningitidis is the causative agent of meningococcal disease. Onset of meningococcal disease can be extremely rapid and can kill within a matter of hours. However, although a much-feared pathogen, Neisseria meningitidis is frequently found in the nasopharyngeal mucosae of healthy carriers. The bacterial fact...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.02001-14
更新日期:2014-12-01 00:00:00
abstract::Two virulence factors of Listeria monocytogenes, listeriolysin O (LLO) and phosphatidylinositol-specific phospholipase C (PI-PLC), mediate escape of this pathogen from the phagocytic vacuole of macrophages, thereby allowing the bacterium access to the host cell cytosol for growth and spread to neighboring cells. We ch...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.10.6639-6646.2005
更新日期:2005-10-01 00:00:00
abstract::Unmethylated CpG dinucleotide motifs present in bacterial genomes or synthetic oligodeoxynucleotides (ODNs) serve as strong immunostimulatory agents in mice, monkeys and humans. We determined the adjuvant effect of murine CpG ODN 1826 on the immunogenicity and protective efficacy of the Saccharomyces cerevisiae-expres...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.72.2.949-957.2004
更新日期:2004-02-01 00:00:00