Abstract:
:In order to deploy virulence factors at appropriate times and locations, microbes must rapidly sense and respond to various metabolite signals. Previously, we showed a transient elevation of the methionine-derived metabolite methylthioadenosine (MTA) concentration in serum during systemic Salmonella enterica serovar Typhimurium infection. Here we explored the functional consequences of increased MTA concentrations on S Typhimurium virulence. We found that MTA, but not other related metabolites involved in polyamine synthesis and methionine salvage, reduced motility, host cell pyroptosis, and cellular invasion. Further, we developed a genetic model of increased bacterial endogenous MTA production by knocking out the master repressor of the methionine regulon, metJ Like MTA-treated S Typhimurium, the ΔmetJ mutant displayed reduced motility, host cell pyroptosis, and invasion. These phenotypic effects of MTA correlated with suppression of flagellar and Salmonella pathogenicity island 1 (SPI-1) networks. S Typhimurium ΔmetJ had reduced virulence in oral and intraperitoneal infection of C57BL/6J mice independently of the effects of MTA on SPI-1. Finally, ΔmetJ bacteria induced a less severe inflammatory cytokine response in a mouse sepsis model. Together, these data indicate that exposure of S Typhimurium to MTA or disruption of the bacterial methionine metabolism pathway suppresses S Typhimurium virulence.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Bourgeois JS,Zhou D,Thurston TLM,Gilchrist JJ,Ko DCdoi
10.1128/IAI.00429-18subject
Has Abstractpub_date
2018-08-22 00:00:00issue
9eissn
0019-9567issn
1098-5522pii
IAI.00429-18journal_volume
86pub_type
杂志文章abstract::Membrane and soluble fractions of Mycoplasma pneumoniae, M. pulmonis, and M. laidlawii B were prepared by hypotonic lysis of whole cells. The membranes of M. pneumoniae and M. laidlawii B contained, as percentage of dry weight: 34 to 37% protein, 59 to 61% lipid, 3 to 4% carbohydrate as hexose, and 0.2% ribonucleic ac...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.2.3.326-339.1970
更新日期:1970-09-01 00:00:00
abstract::We purified three proline-rich antimicrobial peptides from elastase-treated extracts of sheep and goat leukocytes and subjected two of them, OaBac5alpha and ChBac5, to detailed analysis. OaBac5alpha and ChBac5 were homologous to each other and to bovine Bac5. Both exhibited potent, broad-spectrum antimicrobial activit...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.8.4106-4111.1999
更新日期:1999-08-01 00:00:00
abstract::In this study we used Escherichia coli strain F11(P155) of porcine origin. The heat-stable enterotoxin (ST) was produced with a batch fermentor under agitation (500 rpm) and forced aeration (5 liters/min) in Casamino Acids yeast extract medium containing 0.2% glucose. The pH varied from 7.2 to 7.8. The maximum amount ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1980-05-01 00:00:00
abstract::Cultured L929 cells infected with Rickettsia prowazekii had a greatly increased rate of hydrolysis of fatty acid from the oleic acid-radiolabeled phospholipids of the host cell membranes. The incorporation of fatty acid into phospholipid in an infected cell was only moderately inhibited relative to a mock-infected cel...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.1.36-40.1989
更新日期:1989-01-01 00:00:00
abstract::Isolated outer membranes of Borrelia burgdorferi 297 were utilized to obtain partial amino acid sequence information for a low-molecular-weight, outer membrane-associated polypeptide. Degenerate oligonucleotide primers based upon this information were used to amplify a 100-bp probe for detection of the corresponding f...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.2.412-421.1997
更新日期:1997-02-01 00:00:00
abstract::A Pasteurella haemolytica mutant incapable of producing leukotoxin was created by allelic replacement. Concentrated culture supernatants from wild-type P. haemolytica, but not from the mutant, contained the 102-kDa leukotoxin protein and lysed bovine lymphoma cells and sheep erythrocytes. Wild-type P. haemolytica demo...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.8.3209-3212.1995
更新日期:1995-08-01 00:00:00
abstract::Vibrio cholerae, the pathogen that causes cholera, also survives in aqueous reservoirs, probably in the form of biofilms. Quorum sensing negatively regulates V. cholerae biofilm formation through HapR, whose expression is induced at a high cell density. In this study, we show that the concentration of the quorum-sensi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01190-06
更新日期:2007-01-01 00:00:00
abstract::The microfilament inhibitors cytochalasins B and D have been traditionally used to indirectly evaluate the requirement for actin in the uptake of invasive bacterial pathogens by nonprofessional phagocytes. Through their effects on microfilaments, both cytochalasins also impart profound alterations in cellular morpholo...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.10.3187-3193.1990
更新日期:1990-10-01 00:00:00
abstract::Infectious agents are often considered potential triggers of chronic inflammatory disease, including autoimmunity; however, direct evidence is usually lacking. Here we show that following control of acute infection of mice with the myotropic Colombiana strain of Trypanosoma cruzi, parasites persisted in tissue at low ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01497-15
更新日期:2016-03-24 00:00:00
abstract::The cytotoxic effects of bacterial lipopolysaccharides (LPS) on mouse leukocytes have been examined in vivo and in vitro. Intraperitoneal injection of LPS into C57BL/6 mice greatly reduced the recovery of mononuclear cells; LPS was cytotoxic for macrophages, but had a mitogenic effect on lymphocytes. Similar effects o...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.21.1.310-319.1978
更新日期:1978-07-01 00:00:00
abstract::Herein we characterized various genetic markers and the biological behavior of a natural recombinant strain of Toxoplasma gondii (P-Br). From nine genetic markers analyzed, three (B1, ROP1, and SAG1) and three (cS10-A6, GRA6, and SAG3) markers belong to parasites from the type I and type III lineages, respectively. Th...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.11.6392-6401.2003
更新日期:2003-11-01 00:00:00
abstract::In this report, we demonstrate that the variable antigens present on the surface of Plasmodium knowlesi-infected erythrocytes could not be found on the surface of merozoites. A number of technical problems had to be solved to make such a comparative study possible, including the purification of merozoites by affinity ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.33.1.275-284.1981
更新日期:1981-07-01 00:00:00
abstract::This study investigates the role of CD4+ T cells in host defense against cholera enterotoxin-induced diarrhea. Antitoxin immunoglobulin A formation and gut protection against cholera toxin (CT) following oral immunizations with CT were evaluated in normal mice and mice that had been depleted of CD4+ T cells by in vivo...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.10.3630-3638.1991
更新日期:1991-10-01 00:00:00
abstract::The bacterium Neisseria meningitidis is the causative agent of meningitis and sepsis. A generally effective vaccine against N. meningitidis serogroup B is not yet available, but outer membrane vesicle vaccines are in development. These vaccines contain lipopolysaccharide (LPS). The inclusion of N. meningitidis wild-ty...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00846-07
更新日期:2007-12-01 00:00:00
abstract::Pseudomonas aeruginosa bacteremia in cancer patients develops from initial gastrointestinal (GI) colonization with translocation into the bloodstream in the setting of chemotherapy-induced neutropenia and GI mucosal damage. We established a reproducible mouse model of P. aeruginosa GI colonization and systemic spread ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.4.2262-2272.2005
更新日期:2005-04-01 00:00:00
abstract::Formation of compact colonies of Staphylococcus aureus in serum-soft agar is mainly a result of a reaction between protein A and the Fc-part of immunoglobulin G and not a clumping factor-fibrinogen reaction. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.6.4.583-586.1972
更新日期:1972-10-01 00:00:00
abstract::We have developed an improved scheme for the classification of environmental and clinical isolates of Histoplasma capsulatum that is based on analysis of mitochondrial DNA (mtDNA) and ribosomal DNA (rDNA). Strains were initially divided into mtDNA groups according to restriction digests of whole-cell DNA and Southern ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.5.1409-1412.1989
更新日期:1989-05-01 00:00:00
abstract::An exoprotein of Staphylococcus aureus 100 that elicited a positive ileal loop response in the rabbit model was investigated in this study. The protein, as it occurred in the culture supernatant fluid, could be detected initially in the late log phase of growth under aerobic and anaerobic conditions. It was stable und...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.18.2.298-303.1977
更新日期:1977-11-01 00:00:00
abstract::Active immunization with Porphyromonas gingivalis whole-cell preparations has been shown to prevent P. gingivalis infection and oral bone loss. Employing passive antibody transfer and opsonization, we demonstrate with this study that immunization-elicited P. gingivalis-specific immunoglobulin G facilitates clearance o...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.72.4.2408-2411.2004
更新日期:2004-04-01 00:00:00
abstract::Pathogenic Yersinia species utilize a type III secretion system (T3SS) to translocate effectors called Yersinia outer proteins (Yops) into infected host cells. Previous studies demonstrated a role for effector Yops in the inhibition of caspase-1-mediated cell death and secretion of interleukin-1beta (IL-1beta) in naïv...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01695-07
更新日期:2008-09-01 00:00:00
abstract::Immunization with live Plasmodium sporozoites under chloroquine prophylaxis (Spz plus CQ) induces sterile immunity against sporozoite challenge in rodents and, more importantly, in humans. Full protection is obtained with substantially fewer parasites than with the classic immunization with radiation-attenuated sporoz...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01415-09
更新日期:2010-05-01 00:00:00
abstract::In this study we found that serum inhibitory activity against Blastomyces dermatitidis was principally mediated by albumin. This was confirmed in experiments using albumin from several mammalian species. Analbuminemic rat serum did not inhibit B. dermatitidis growth in vivo; however, the addition of albumin restored i...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.11.6648-6652.2003
更新日期:2003-11-01 00:00:00
abstract::Two extremely oxygen-sensitive strains of Clostridium sp., designated Clostridium E and P, were obtained from digestive microflora of conventional mice and found to constitute a barrier against Shigella flexneri SF-2 when associated in vivo with Escherichia coli K-12. These and other simplified fractions of the conven...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.17.2.415-424.1977
更新日期:1977-08-01 00:00:00
abstract::Patients with chronic bronchitis are colonized by and may develop acute bronchopulmonary infection due to nontypable Haemophilus influenzae (NTHI) despite the presence of bactericidal and opsonizing antibody to the infecting organism. To test the hypothesis that secretory immunoglobulin A (IgA) interferes with host de...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.45.1.36-40.1984
更新日期:1984-07-01 00:00:00
abstract::Plasmodium falciparum's ability to invade erythrocytes is essential for its survival within the human host. Immune mechanisms that impair this ability are therefore expected to contribute to immunity against the parasite. Plasma of humans who are naturally exposed to malaria has been shown to have growth-inhibitory ac...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.06190-11
更新日期:2012-05-01 00:00:00
abstract::V beta 10(+) and V beta 14(+) T cells were selectively increased 7 to 14 days following infection in the lungs of naive mice infected with Histoplasma capsulatum. Following secondary challenge of immune mice, V beta 1(+) and V beta 8.1(+) cells were sporadically increased. Elimination of V beta 10(+) and V beta 14(+) ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.3.1648-1652.2002
更新日期:2002-03-01 00:00:00
abstract::CmeABC functions as a multidrug efflux pump contributing to the resistance of Campylobacter to a broad range of antimicrobials. In this study, we examined the role of CmeABC in bile resistance and its contribution to the adaptation of Campylobacter jejuni in the intestinal tract of the chicken, a natural host and a ma...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.8.4250-4259.2003
更新日期:2003-08-01 00:00:00
abstract::Escherichia coli containing the K1 capsule is the leading cause of gram-negative meningitis, but the pathogenesis of this disease is not completely understood. Recent microarray experiments in which we compared the gene expression profile of E. coli K1 associated with human brain microvascular endothelial cells (HBMEC...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01543-06
更新日期:2007-06-01 00:00:00
abstract::The contribution of murine Toll-like receptors 2 and 4 (TLR2 and -4, respectively) to cytokine induction by heat-killed bacteria was analyzed in vitro and in vivo. Gram-negative bacteria induced cytokines primarily via TLR4; the contribution of TLR2 was only minor. Neither TLR4 nor, surprisingly, TLR2 was required in ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.10.6058-6062.2003
更新日期:2003-10-01 00:00:00
abstract::Gonococci do not readily cause disseminated infection in mice. To simulate some of the conditions leading to disseminated gonococcal infection in women, we suspended gonococci in mucin plus hemoglobin and studied the development of gonococcal bacteremia. The mucin-hemoglobin mixture was used because the menstruum appe...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.26.3.984-990.1979
更新日期:1979-12-01 00:00:00