Abstract:
:In the immune system, T- and B-cell proliferation, as well as B-cell immunoglobulin secretion, is induced by interleukin-2 (IL-2), a T-cell-derived lymphokine. IL-2 also influences the growth of glial cells, specifically, the proliferation and maturation of oligodendrocytes. Studies were conducted to investigate further IL-2-induced maturation of oligodendrocytes through its effect on the regulation of the myelin basic protein (MBP) gene. A cDNA probe specific for rat MBP and a double-antibody radioimmunoassay for MBP were used to quantitate MBP mRNA and protein levels in oligodendrocytes under different experimental conditions. We demonstrate that both MBP mRNA and protein levels are increased in IL-2-stimulated oligodendrocytes. MBP mRNA levels increase within 8 h after IL-2 stimulation, peak between 24 and 48 h, and then decline slightly. MBP protein levels increase 24 h after stimulation and peak at 72 h. MBP mRNA transcripts in the range of 2.0-2.4 kilobases are present in cultured rat oligodendrocytes, which are similar to the MBP mRNA transcripts detected in whole rat and mouse brain. These mRNA transcripts are specifically increased in quantity after oligodendrocyte stimulation with IL-2. These results suggest that one component of oligodendrocyte differentiation/maturation--MBP mRNA and protein expression--can be regulated in part by IL-2.
journal_name
J Neurochemjournal_title
Journal of neurochemistryauthors
Benveniste EN,Herman PK,Whitaker JNdoi
10.1111/j.1471-4159.1987.tb10020.xsubject
Has Abstractpub_date
1987-10-01 00:00:00pages
1274-9issue
4eissn
0022-3042issn
1471-4159journal_volume
49pub_type
杂志文章abstract::Muscarinic receptors are the predominant cholinergic receptors in the central and peripheral nervous systems. Recently, activation of muscarinic receptors was found to elicit pigment granule dispersion in retinal pigment epithelium isolated from bluegill fish. Pigment granule movement in retinal pigment epithelium is ...
journal_title:Journal of neurochemistry
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journal_title:Journal of neurochemistry
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journal_title:Journal of neurochemistry
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journal_title:Journal of neurochemistry
pub_type: 杂志文章
doi:10.1046/j.1471-4159.1996.67020473.x
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abstract::The Review highlighted in this Editorial followed a CAEN Return Home Grant. ...
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journal_title:Journal of neurochemistry
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journal_title:Journal of neurochemistry
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journal_title:Journal of neurochemistry
pub_type: 杂志文章
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更新日期:1991-04-01 00:00:00
abstract::Previous studies showed that prolonged access to cocaine or heroin self-administration (long access, or LgA) produces an escalation in drug intake not observed with limited access to the drug (short access, or ShA). The present experiment employed in vivo microdialysis to test the role of alterations in drug pharmacok...
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journal_title:Journal of neurochemistry
pub_type: 杂志文章
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