Binding interactions of leukemia inhibitory factor and ciliary neurotrophic factor with the different subunits of their high affinity receptors.


:Leukemia inhibitory factor (LIF) and ciliary neurotrophic factor (CNTF) share common components in their multimeric receptors. Both cytokine receptors contain gp130/interleukin-6-receptor transducer as well as gp190/low affinity LIF receptor. For CNTF, addition of a third subunit, or alpha subunit, defines the high-affinity CNTF receptor. In the present study, we analyzed the binding interactions of LIF and CNTF in human cell lines and showed a mutual displacement for LIF and CNTF toward the trimeric high-affinity CNTF receptor. Similar results were obtained in the JEG cell line, which only expressed the gp130/gp190 high-affinity LIF receptor, by adding a soluble form of the alpha CNTF receptor to the system to reconstitute the high-affinity-type CNTF receptor. The different receptor subunits were then expressed separately in transfected cells and their binding capacities analyzed. The results showed that the heterocomplex CNTF/alpha CNTF receptor bound to gp130 with an affinity of 3-5 x 10(-10)M, whereas LIF interacted mainly with gp190. In summary, the observed competition between LIF and CNTF does not result from the binding to a common site or receptor subunit, but rather to the interaction of the three receptor components to create a conformational site common to both LIF and CNTF.


J Neurochem


Robledo O,Auguste P,Coupey L,Praloran V,Chevalier S,Pouplard A,Gascan H




Has Abstract


1996-04-01 00:00:00












  • Identification and transgenic analysis of a murine promoter that targets cholinergic neuron expression.

    abstract::Choline acetyltransferase (ChAT) is a specific phenotypic marker of cholinergic neurons. Previous reports showed that different upstream regions of the ChAT gene are necessary for cell type-specific expression of reporter genes in cholinergic cell lines. The identity of the mouse ChAT promoter region controlling the e...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Naciff JM,Behbehani MM,Misawa H,Dedman JR

    更新日期:1999-01-01 00:00:00

  • SCF-Slimb is critical for Glycogen synthase kinase-3β-mediated suppression of TAF15-induced neurotoxicity in Drosophila.

    abstract::Amyotrophic lateral sclerosis (ALS) is a severe neurodegenerative disorder characterized pathologically by motor neuron degeneration and associated with aggregation of RNA-binding proteins. TATA-binding protein-associated factor 15 (TAF15) accumulates as cytoplasmic aggregates in neuronal cells, and clearance of these...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Choi HJ,Joo Cha S,Do HA,Kim HJ,Lee JW,Kim K

    更新日期:2020-09-11 00:00:00

  • K-252b is a selective and nontoxic inhibitor of nerve growth factor action on cultured brain neurons.

    abstract::K-252b is a kinase inhibitor structurally related to K-252a, which is known to abolish selectively the effects of nerve growth factor (NGF) on PC12 cells and PNS neurons. We tested whether K-252b, K-252a, and staurosporine, another related compound, are effective and selective inhibitors of NGF actions on CNS neurons....

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Knüsel B,Hefti F

    更新日期:1991-09-01 00:00:00

  • Coupling gene chip analyses and rat genetic variances in identifying potential target genes that may contribute to neuropathic allodynia development.

    abstract::Genetic factors and nerve injury-induced changes of gene expression in sensory neurons are potential contributors to tactile allodynia, a neuropathic pain state manifested as hypersensitivity to innocuous mechanical stimulation. To uncover genes relevant to neuropathic allodynia, we analyzed gene expression profiles i...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Valder CR,Liu JJ,Song YH,Luo ZD

    更新日期:2003-11-01 00:00:00

  • Nongenomic inhibition of catecholamine secretion by 17beta-estradiol in PC12 cells.

    abstract::We investigated the effects of 17beta-estradiol, an estrogen, on [(3)H]norepinephrine ([(3)H]NE) secretion in PC12 cells. Pretreatment with 17beta-estradiol reduced 70 mM K(+)-induced [(3)H]NE secretion in a concentration-dependent manner with a half-maximal inhibitory concentration (IC(50)) of 2 +/- 1 microM. The 70 ...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Kim YJ,Hur EM,Park TJ,Kim KT

    更新日期:2000-06-01 00:00:00

  • CaMKII associates with CaV1.2 L-type calcium channels via selected beta subunits to enhance regulatory phosphorylation.

    abstract::Calcium/calmodulin-dependent kinase II (CaMKII) facilitates L-type calcium channel (LTCC) activity physiologically, but may exacerbate LTCC-dependent pathophysiology. We previously showed that CaMKII forms stable complexes with voltage-gated calcium channel (VGCC) beta(1b) or beta(2a) subunits, but not with the beta(3...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Abiria SA,Colbran RJ

    更新日期:2010-01-01 00:00:00

  • Early stress and chronic methylphenidate cross-sensitize dopaminergic responses in the adolescent medial prefrontal cortex and nucleus accumbens.

    abstract::Methylphenidate (MP) is widely used to treat attention deficit/hyperactivity disorder in children. However, basic research has been mainly focused on MP treatment in adult, behaviorally normal rodents. Here we analyzed MP-evoked changes of dopamine (DA) release in the limbic system of juvenile rodents with hyperactive...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Jezierski G,Zehle S,Bock J,Braun K,Gruss M

    更新日期:2007-12-01 00:00:00

  • Caspase activation accompanying cytochrome c release from mitochondria is possibly involved in nitric oxide-induced neuronal apoptosis in SH-SY5Y cells.

    abstract::It is well known that caspases are produced as proforms, which are proteolytically cleaved and activated during apoptosis or programmed cell death. We report here that caspases are activated during apoptosis by treatment with NOC18, a nitric oxide (NO) donor. Our present experiments have examined the way in which NO i...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Uehara T,Kikuchi Y,Nomura Y

    更新日期:1999-01-01 00:00:00

  • In vivo assessment of riluzole as a potential therapeutic drug for spinocerebellar ataxia type 3.

    abstract::Spinocerebellar ataxia type 3 (SCA3) is an autosomal dominantly inherited neurodegenerative disorder for which no curative therapy is available. The cause of this disease is the expansion of a CAG repeat in the so-called ATXN3 gene leading to an expanded polyglutamine stretch in the ataxin-3 protein. Although the func...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Schmidt J,Schmidt T,Golla M,Lehmann L,Weber JJ,Hübener-Schmid J,Riess O

    更新日期:2016-07-01 00:00:00

  • Mitochondrial membrane depolarization and the selective death of dopaminergic neurons by rotenone: protective effect of coenzyme Q10.

    abstract::Chronic exposure to the pesticide rotenone induces a selective degeneration of nigrostriatal dopaminergic neurons and reproduces the features of Parkinson's disease in experimental animals. This action is thought to be relevant to its inhibition of the mitochondrial complex I, but the precise mechanism of this suppres...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Moon Y,Lee KH,Park JH,Geum D,Kim K

    更新日期:2005-06-01 00:00:00

  • A unique snake venom neuritogenesis mechanism: A cornerstone in the treatment of neurodegenerative diseases?: An Editorial Highlight for "Transcriptomic, proteomic, and biochemical analyses reveal a novel neuritogenesis mechanism of Naja naja venom α-elap

    abstract::Neurodegenerative diseases are a worldwide health problem and are a major cause of death and disability. A progressive loss of defined neuronal populations is triggered by a diverse array of stimuli that converge in deficient neurotrophic signaling. Therefore, much effort has been placed in recent years in the charact...

    journal_title:Journal of neurochemistry

    pub_type: 社论


    authors: Hernández-Kelly LC,Ortega A

    更新日期:2020-10-19 00:00:00

  • Chronic stress elicits prolonged activation of alpha-MSH secretion and subsequent degeneration of melanotroph.

    abstract::Prolonged stress affects homeostasis in various organs and induces stress-associated disorders. We examined the cellular changes of pituitary gland under the continuous stress condition using a rat model in which rats were kept in a cage filled with water to a height of 1.5 cm for up to 5 days. Among the pituitary hor...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Ogawa T,Shishioh-Ikejima N,Konishi H,Makino T,Sei H,Kiryu-Seo S,Tanaka M,Watanabe Y,Kiyama H

    更新日期:2009-06-01 00:00:00

  • Characterization of [3H]quipazine binding to 5-hydroxytryptamine3 receptors in rat brain membranes.

    abstract::[3H]Quipazine was used to label binding sites in rat brain membranes that display characteristics of a 5-hydroxytryptamine3 (5-HT3) receptor. The radioligand binds with high affinity (KD, 1.2 +/- 0.1 nM) to a saturable population of sites (Bmax, 3.0 +/- 0.4 pmol/g of tissue) that are differentially located in the brai...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Milburn CM,Peroutka SJ

    更新日期:1989-06-01 00:00:00

  • Fingolimod confers neuroprotection through activation of Rac1 after experimental germinal matrix hemorrhage in rat pups.

    abstract::Fingolimod, a sphingosine-1-phosphate receptor (S1PR) agonist, is clinically available to treat multiple sclerosis and is showing promise in treating stroke. We investigated if fingolimod provides long-term protection from experimental neonatal germinal matrix hemorrhage (GMH), aiming to support a potential mechanism ...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Rolland WB,Krafft PR,Lekic T,Klebe D,LeGrand J,Weldon AJ,Xu L,Zhang JH

    更新日期:2017-03-01 00:00:00

  • Dolichol is the major lipid component of human substantia nigra neuromelanin.

    abstract::Neuromelanin is a dark brown pigment present at high concentrations in dopaminergic neurones of the human substantia nigra (SN). Early electron microscopic examinations of neuromelanin fine structure revealed a significant neutral lipid component; however, the identity of this lipid has remained unknown. Here we show ...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Fedorow H,Pickford R,Hook JM,Double KL,Halliday GM,Gerlach M,Riederer P,Garner B

    更新日期:2005-02-01 00:00:00

  • Dynorphin A(1-17) biotransformation in striatum of freely moving rats using microdialysis and matrix-assisted laser desorption/ionization mass spectrometry.

    abstract::The biotransformation of the opioid peptide dynorphin A(1-17) was investigated in striatum of freely moving Fischer rats, by direct infusion of this peptide, followed by recovery of the resulting biotransformation products via microdialysis and identification using matrix-assisted laser desorption/ionization mass spec...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Reed B,Zhang Y,Chait BT,Kreek MJ

    更新日期:2003-08-01 00:00:00

  • Role of basic region leucine zipper transcription factors cyclic AMP response element binding protein (CREB), CREB2, activating transcription factor 2 and CAAT/enhancer binding protein alpha in cyclic AMP response element-mediated transcription.

    abstract::The transcription factor cAMP response element binding protein (CREB), a member of the basic region leucine zipper (bZIP) family of proteins, is the major cAMP response element (CRE) binding. Other bZIP proteins, including CREB2, activating transcription factor 2 (ATF2), or CAAT/enhancer binding protein (C/EBP) have b...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Thiel G,Al Sarraj J,Vinson C,Stefano L,Bach K

    更新日期:2005-01-01 00:00:00

  • Activation of metabotropic glutamate receptors in the hippocampus increases cyclic AMP accumulation.

    abstract::The selective metabotropic glutamate receptor agonist trans-1-aminocyclopentane-1,3-dicarboxylic acid (trans-ACPD) stimulates phosphoinositide hydrolysis and elicits several physiological responses in rat hippocampal slices. However, recent studies suggest that the physiological effects of trans-ACPD in the hippocampu...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Winder DG,Conn PJ

    更新日期:1992-07-01 00:00:00

  • Characterization of an initiating cell-free protein synthesis system derived from rabbit brain.

    abstract::Protein synthesis in the brain is known to be affected by a wide range of treatments. The detailed analysis of the mechanisms that are involved would be facilitated by the development of cell-free translation systems derived from brain tissue. To date, brain cell-free systems have not been fully characterized to demon...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Cosgrove JW,Brown IR

    更新日期:1981-03-01 00:00:00

  • gdnf affects early diencephalic dopaminergic neuron development through regulation of differentiation-associated transcription factors in zebrafish.

    abstract::Glial cell line-derived neurotrophic factor (GDNF) has been reported to enhance dopaminergic neuron survival and differentiation in vitro and in vivo, although those results are still being debated. Glial cell line-derived neurotrophic factor (gdnf) is highly conserved in zebrafish and plays a role in enteric nervous ...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Wong CED,Hua K,Monis S,Saxena V,Norazit A,Noor SM,Ekker M

    更新日期:2020-06-24 00:00:00

  • Benzimidazole-based fluorophores for the detection of amyloid fibrils with higher sensitivity than Thioflavin-T.

    abstract::Protein aggregation into amyloid fibrils is a key feature of a multitude of neurodegenerative diseases such as Alzheimer's, Parkinson's, and Prion disease. To detect amyloid fibrils, fluorophores with high sensitivity and better efficiency coupled with the low toxicity are in high demand even to date. In this pursuit,...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Pravin N,Kumar R,Tripathi S,Kumar P,Mohite GM,Navalkar A,Panigrahi R,Singh N,Gadhe LG,Manchanda S,Shimozawa M,Nilsson P,Johansson J,Kumar A,Maji SK,Shanmugam M

    更新日期:2020-08-04 00:00:00

  • Neprilysin activity and expression are controlled by nicastrin.

    abstract::We recently demonstrated that the presenilin-dependent gamma-secretase complex regulates the expression and activity of neprilysin, one of the main enzymes that degrade the amyloid beta-peptide (Abeta) which accumulates in Alzheimer's disease. Here, we examined the influence of endogenous nicastrin (NCT), a member of ...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Pardossi-Piquard R,Dunys J,Yu G,St George-Hyslop P,Alves da Costa C,Checler F

    更新日期:2006-05-01 00:00:00

  • Basement membrane proteins influence brain capillary endothelial barrier function in vitro.

    abstract::The influence of basement membrane proteins on cellular barrier properties of primary cultures of porcine brain capillary endothelial cells grown on permeable filter inserts has been investigated. Measurements of transcellular electrical resistance (TER) by impedance spectroscopy were performed with cells cultured on ...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Tilling T,Korte D,Hoheisel D,Galla HJ

    更新日期:1998-09-01 00:00:00

  • Guanine nucleotide regulation of [125I]beta-endorphin binding to rat brain membranes: monovalent cation requirement.

    abstract::The binding of [125I]beta h-endorphin to rat brain membranes was investigated in the presence of GTP and guanylyl-5'-imidodiphosphate. In contrast to the binding of the mu-selective opioid agonist, [3H][D-Ala2,MePhe4,Glyol5]enkephalin, and the delta-selective opioid agonist, [3H][D-penicillamine2, D-penicillamine5]enk...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Selley DE,Tyler CB,Bidlack JM

    更新日期:1988-06-01 00:00:00

  • Effect of pertussis toxin on radioligand binding to rat brain adenosine A1 receptors.

    abstract::In a previous study we showed that in vivo treatment with pertussis toxin could inhibit some, but not all, effects of adenosine in the rat hippocampus. In this study we investigated the effect of pertussis toxin on the binding of adenosine analogues to A1 receptors in rat brain. Intraventricular injection of pertussis...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: van der Ploeg I,Parkinson FE,Fredholm BB

    更新日期:1992-04-01 00:00:00

  • Regulated intramembrane proteolysis--lessons from amyloid precursor protein processing.

    abstract::Regulated intramembrane proteolysis (RIP) controls the communication between cells and the extracellular environment. RIP is essential in the nervous system, but also in other tissues. In the RIP process, a membrane protein typically undergoes two consecutive cleavages. The first one results in the shedding of its ect...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章,评审


    authors: Lichtenthaler SF,Haass C,Steiner H

    更新日期:2011-06-01 00:00:00

  • Sarco-endoplasmic reticulum Ca2+ ATPase (SERCA) inhibitors identify a novel calcium pool in the central nervous system.

    abstract::Ca2+ uptake into the endoplasmic reticulum (ER) is mediated by Ca2+ ATPase isoforms, which are all selectively inhibited by nanomolar concentrations of thapsigargin. Using ATP/Mg2+-dependent 45Ca2+ transport in rat brain microsomes, tissue sections, and permeabilized cells, as well as Ca2+ imaging in living cells we d...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Watson WD,Facchina SL,Grimaldi M,Verma A

    更新日期:2003-10-01 00:00:00

  • Fractalkine-induced activation of the phosphatidylinositol-3 kinase pathway attentuates microglial activation in vivo and in vitro.

    abstract::Several neurodegenerative disorders are associated with evidence of inflammation, one feature of which is increased activation of microglia, the most likely cellular source of inflammatory cytokines like interleukin-1beta. It is now recognized that interaction of microglia with other cells contributes to maintenance o...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Lyons A,Lynch AM,Downer EJ,Hanley R,O'Sullivan JB,Smith A,Lynch MA

    更新日期:2009-09-01 00:00:00

  • Sequence and novel distribution of the chicken homologue of the mammalian gamma-aminobutyric acidA receptor gamma 1 subunit.

    abstract::cDNAs have been cloned that encode the chicken (Gallus domesticus) gamma-aminobutyric acidA receptor gamma 1 subunit, the mature sequence of which shares 90, 79, and 69% identity with those of the rat gamma 1, gamma 2, and gamma 3 subunits, respectively. In situ hybridization reveals that there are pronounced differen...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Glencorse TA,Darlison MG,Barnard EA,Bateson AN

    更新日期:1993-12-01 00:00:00

  • Depolarization of cerebellar granule cells increases phosphorylation of rabphilin-3A.

    abstract::Studies performed over the past several years have provided evidence that phosphorylation of proteins is important in the regulation of neurotransmitter release. In this study, it is shown that rabphilin-3A is present in cerebellar granule cells as a phosphoprotein, by using 32P-labeling of cerebellar granule cells, i...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Fykse EM

    更新日期:1998-10-01 00:00:00