Binding interactions of leukemia inhibitory factor and ciliary neurotrophic factor with the different subunits of their high affinity receptors.


:Leukemia inhibitory factor (LIF) and ciliary neurotrophic factor (CNTF) share common components in their multimeric receptors. Both cytokine receptors contain gp130/interleukin-6-receptor transducer as well as gp190/low affinity LIF receptor. For CNTF, addition of a third subunit, or alpha subunit, defines the high-affinity CNTF receptor. In the present study, we analyzed the binding interactions of LIF and CNTF in human cell lines and showed a mutual displacement for LIF and CNTF toward the trimeric high-affinity CNTF receptor. Similar results were obtained in the JEG cell line, which only expressed the gp130/gp190 high-affinity LIF receptor, by adding a soluble form of the alpha CNTF receptor to the system to reconstitute the high-affinity-type CNTF receptor. The different receptor subunits were then expressed separately in transfected cells and their binding capacities analyzed. The results showed that the heterocomplex CNTF/alpha CNTF receptor bound to gp130 with an affinity of 3-5 x 10(-10)M, whereas LIF interacted mainly with gp190. In summary, the observed competition between LIF and CNTF does not result from the binding to a common site or receptor subunit, but rather to the interaction of the three receptor components to create a conformational site common to both LIF and CNTF.


J Neurochem


Robledo O,Auguste P,Coupey L,Praloran V,Chevalier S,Pouplard A,Gascan H




Has Abstract


1996-04-01 00:00:00












  • Dimercaprol is an acrolein scavenger that mitigates acrolein-mediated PC-12 cells toxicity and reduces acrolein in rat following spinal cord injury.

    abstract::Acrolein is one of the most toxic byproducts of lipid peroxidation, and it has been shown to be associated with multiple pathological processes in trauma and diseases, including spinal cord injury, multiple sclerosis, and Alzheimer's disease. Therefore, suppressing acrolein using acrolein scavengers has been suggested...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Tian R,Shi R

    更新日期:2017-06-01 00:00:00

  • Functional impairment in protein kinase C by RACK1 (receptor for activated C kinase 1) deficiency in aged rat brain cortex.

    abstract::Several laboratories have reported a lack of protein kinase C (PKC) activation in response to various stimuli in the brain of aged rats. It has been suggested that changes in lipid membrane composition could be related to this functional deficit. However, recent evidence has indicated that the translocation of PKC to ...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Pascale A,Fortino I,Govoni S,Trabucchi M,Wetsel WC,Battaini F

    更新日期:1996-12-01 00:00:00

  • Enhancing the GLP-1 receptor signaling pathway leads to proliferation and neuroprotection in human neuroblastoma cells.

    abstract::Increasing evidence suggests that glucagon-like peptide-1 (GLP-1), an incretin hormone of current interest in type 2 diabetes, is neuroprotective in both cell culture and animal models. To characterize the neuroprotective properties of GLP-1 and associated underlying mechanisms, we over-expressed the GLP-1 receptor (G...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Li Y,Tweedie D,Mattson MP,Holloway HW,Greig NH

    更新日期:2010-06-01 00:00:00

  • A kainate receptor linked to nitric oxide synthesis from arginine.

    abstract::In slices of young rat cerebellum, the glutamate analogue kainate induced a large accumulation of cyclic GMP, which was inhibited by non-N-methyl-D-aspartate antagonists. Quisqualate and alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate evoked only small cyclic GMP responses and inhibited the effect of kainate. Whe...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Garthwaite J,Southam E,Anderton M

    更新日期:1989-12-01 00:00:00

  • Pituitary adenylate cyclase-activating polypeptide (PACAP) stimulates the expression and the release of tissue plasminogen activator (tPA) in neuronal cells: involvement of tPA in the neuroprotective effect of PACAP.

    abstract::Pituitary adenylate cyclase-activating polypeptide (PACAP) and tissue plasminogen activator (tPA) play important roles in neuronal migration and survival. However, a direct link between the neurotrophic effects of PACAP and tPA has never been investigated. In this study, we show that, in PC12 cells, PACAP induced a 9....

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Raoult E,Roussel BD,Bénard M,Lefebvre T,Ravni A,Ali C,Vivien D,Komuro H,Fournier A,Vaudry H,Vaudry D,Galas L

    更新日期:2011-12-01 00:00:00

  • Dopamine neurons in culture express VGLUT2 explaining their capacity to release glutamate at synapses in addition to dopamine.

    abstract::Dopamine neurons have been suggested to use glutamate as a cotransmitter. To identify the basis of such a phenotype, we have examined the expression of the three recently identified vesicular glutamate transporters (VGLUT1-3) in postnatal rat dopamine neurons in culture. We found that the majority of isolated dopamine...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Dal Bo G,St-Gelais F,Danik M,Williams S,Cotton M,Trudeau LE

    更新日期:2004-03-01 00:00:00

  • Postmortem changes in catecholamines, indoleamines, and their metabolites in rat brain regions: prevention with 10-kW microwave irradiation.

    abstract::Postmortem changes in catecholamines, indoleamines, and their metabolites in rat brain regions following decapitation were determined by LCEC. In the three regions studied neurotransmitter levels declined after decapitation, whereas the metabolite levels increased. Microwave irradiation at 10 kW rapidly inactivated br...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Ikarashi Y,Sasahara T,Maruyama Y

    更新日期:1985-09-01 00:00:00

  • The phosphorylation status of extracellular-regulated kinase 1/2 in astrocytes and neurons from rat hippocampus determines the thrombin-induced calcium release and ROS generation.

    abstract::Challenge of protease-activated receptors induces cytosolic Ca(2+) concentration ([Ca(2+) ](c)) increase, mitogen-activated protein kinase activation and reactive oxygen species (ROS) formation with a bandwidth of responses in individual cells. We detected in this study in situ the thrombin-induced [Ca(2+) ](c) rise a...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Zündorf G,Reiser G

    更新日期:2011-12-01 00:00:00

  • Pre- and early postnatal enriched environmental experiences prevent neonatal hypoxia-ischemia late neurodegeneration via metabolic and neuroplastic mechanisms.

    abstract::Prenatal and early postnatal periods are important for brain development and neural function. Neonatal insults such as hypoxia-ischemia (HI) causes prolonged neural and metabolic dysregulation, affecting central nervous system maturation. There is evidence that brain hypometabolism could increase the risk of adult-ons...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Durán-Carabali LE,Kawa Odorcyk F,Greggio S,Teribele Venturin G,Farias Sanches E,Garcia Schu G,Soares Carvalho A,Avila Pedroso T,de Sá Couto-Pereira N,Costa da Costa J,Dalmaz C,Rigon Zimmer E,Netto CA

    更新日期:2020-10-23 00:00:00

  • Mimicking the nicotinic receptor binding site by a single chain Fv selected by competitive panning from a synthetic phage library.

    abstract::We have developed a novel competitive method to select from a phage display library a single chain Fv which is able to mimic the alpha-bungarotoxin binding site of the muscle nicotinic receptor. The single chain Fv was selected from a large synthetic library using alpha-bungarotoxin-coated magnetic beads. Toxin-bound ...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Bracci L,Pini A,Lozzi L,Lelli B,Battestin P,Spreafico A,Bernini A,Niccolai N,Neri P

    更新日期:2001-07-01 00:00:00

  • Shift in the ratio of three-repeat tau and four-repeat tau mRNAs in individual cholinergic basal forebrain neurons in mild cognitive impairment and Alzheimer's disease.

    abstract::Molecular mechanisms underlying tauopathy remain undetermined. In the current study, single cell gene expression profiling was coupled with custom-designed cDNA array analysis to evaluate tau expression and other cytoskeletal elements within individual neuronal populations in patients with no cognitive impairment (NCI...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Ginsberg SD,Che S,Counts SE,Mufson EJ

    更新日期:2006-03-01 00:00:00

  • Effects of transforming growth factor-beta and platelet-derived growth factor on oligodendrocyte precursors: insights gained from a neuronal cell line.

    abstract::Conditioned medium derived from a rat central nervous system neuronal cell line B104 (B104 CM) was shown previously to contain uncharacterized potent mitogen(s) for oligodendrocyte/type-2 astrocyte (O-2A) progenitor cells. In this study, we demonstrated that B104 cells produce and secrete platelet-derived growth facto...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Asakura K,Hunter SF,Rodriguez M

    更新日期:1997-06-01 00:00:00

  • Inhibition by L-phenylalanine of tyrosine transport by synaptosomal plasma membrane vesicles: implications in the pathogenesis of phenylketonuria.

    abstract::The effect of L-phenylalanine on the transport of tyrosine was studied using membrane vesicles from rat brain synaptosomes. Phenylalanine, which is accumulated in phenylketonuria, competitively inhibits tyrosine uptake at concentrations similar to those found in phenylketonuric patients, with a Ki of the same order of...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Aragón MC,Giménez C,Valdivieso F

    更新日期:1982-10-01 00:00:00

  • Sp1 and Sp3 activate transcription of the human dopamine transporter gene.

    abstract::The dopamine transporter is a plasma membrane protein that controls extracellular concentrations of the neurotransmitter dopamine. The physiological importance of the DAT provides the impetus for studies aimed at understanding the molecular mechanisms underlying regulation of the DAT gene. In this study, we identified...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Wang J,Bannon MJ

    更新日期:2005-04-01 00:00:00

  • Effect of acute stress on hippocampal glutamate levels and spectrin proteolysis in young and aged rats.

    abstract::Aging in rats is associated with a loss of hippocampal neurons, which may contribute to age-related cognitive deficits. Several lines of evidence suggest that stress and glucocorticoids may contribute to age-related declines in hippocampal neuronal number. Excitatory amino acids (EAAs) have been implicated in the gluc...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Lowy MT,Wittenberg L,Yamamoto BK

    更新日期:1995-07-01 00:00:00

  • Dynamic aspects of glucocorticoid receptors in the spinal cord of the rat.

    abstract::In spite of biochemical and autoradiographic evidence for glucocorticoid binding sites in the spinal cord (SC), events occurring after the preliminary step of hormone binding were not studied. In this investigation, we have examined the transformation (activation) of the cytosolic receptor coupled to [3H]dexamethasone...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Ortí E,Tornello S,De Nicola AF

    更新日期:1985-12-01 00:00:00

  • Glucocorticoids exacerbate the deleterious effects of gp120 in hippocampal and cortical explants.

    abstract::Glucocorticoids (GCs), the adrenal steroids secreted during stress, can compromise the ability of hippocampal neurons to survive numerous necrotic insults. We have previously observed that GCs worsen the deleterious effects of gp120, the glycoprotein of the acquired immune deficiency syndrome virus, which can indirect...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Yusim A,Franklin L,Brooke S,Ajilore O,Sapolsky R

    更新日期:2000-03-01 00:00:00

  • Genomic profiling of the neuronal target genes of the plasticity-related transcription factor -- Zif268.

    abstract::The later phases of neuronal plasticity are invariably dependent on gene transcription. Induction of the transcription factor Zif268 (Egr-1) in neurones is closely associated with many forms of functional plasticity, yet the neuronal target genes modulated by Zif268 have not been characterized. After transfection of a...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: James AB,Conway AM,Morris BJ

    更新日期:2005-11-01 00:00:00

  • Metorphamide levels in chromaffin cells increase after treatment with reserpine.

    abstract::Exposure of bovine chromaffin cells in primary culture to 0.01-1 microM reserpine caused a dose- and time-dependent increase in intracellular levels of the amidated enkephalin peptide metorphamide. Maximal levels (approximately 800% of control) were obtained at 0.1 microM reserpine and increased levels were apparent b...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Eiden LE,Zamir N

    更新日期:1986-05-01 00:00:00

  • Uptake of amino acids by brain microvessels isolated from rats after portacaval anastomosis.

    abstract::The uptake of amino acids by microvessels isolated from brains of rats was studied. Previous studies have demonstrated alterations in blood-brain amino acid transport after portacaval shunt in rats. In order to elucidate whether such changes in the blood-brain barrier were located in the microvessels, brain microvesse...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Cardelli-Cangiano P,Cangiano C,James JH,Jeppsson B,Brenner W,Fischer JE

    更新日期:1981-02-01 00:00:00

  • Bdnf gene is a downstream target of Nurr1 transcription factor in rat midbrain neurons in vitro.

    abstract::The transcription factor Nurr1 is essential for the generation of midbrain dopaminergic neurons (mDA). Only a few Nurr1-regulated genes have so far been identified and it remains unclear how Nurr1 influences the development and function of dopaminergic neurons. To identify novel Nurr1 target genes we have used genome-...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Volpicelli F,Caiazzo M,Greco D,Consales C,Leone L,Perrone-Capano C,Colucci D'Amato L,di Porzio U

    更新日期:2007-07-01 00:00:00

  • Resveratrol mitigates lipopolysaccharide- and Aβ-mediated microglial inflammation by inhibiting the TLR4/NF-κB/STAT signaling cascade.

    abstract::Activation of microglia, the resident macrophages of the brain, around the amyloid plaques is a key hallmark of Alzheimer's disease (AD). Recent evidence in mouse models indicates that microglia are required for the neurodegenerative process of AD. Amyloid-β (Aβ) peptides, the core components of the amyloid plaques, c...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Capiralla H,Vingtdeux V,Zhao H,Sankowski R,Al-Abed Y,Davies P,Marambaud P

    更新日期:2012-02-01 00:00:00

  • Neurotrophic actions initiated by proNGF in adult sensory neurons may require peri-somatic glia to drive local cleavage to NGF.

    abstract::The nerve growth factor (NGF) precursor, proNGF, is implicated in various neuropathological states. ProNGF signals apoptosis by forming a complex with the receptors p75 and sortilin, however, it can also induce neurite growth, proposed to be mediated by the receptor of mature NGF, tyrosine kinase receptor A (TrkA). Th...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Kalous A,Nangle MR,Anastasia A,Hempstead BL,Keast JR

    更新日期:2012-08-01 00:00:00

  • Cerebrospinal fluid total tau concentration predicts clinical phenotype in Huntington's disease.

    abstract::Huntington's disease (HD) is a hereditary neurodegenerative condition with no therapeutic intervention known to alter disease progression, but several trials are ongoing and biomarkers of disease progression are needed. Tau is an axonal protein, often altered in neurodegeneration, and recent studies pointed out its ro...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章,多中心研究


    authors: Rodrigues FB,Byrne L,McColgan P,Robertson N,Tabrizi SJ,Leavitt BR,Zetterberg H,Wild EJ

    更新日期:2016-10-01 00:00:00

  • S-100 and other acidic proteins promote Ca2+-independent phosphorylation of protamine catalyzed by a new protein kinase from brain.

    abstract::A new protein kinase modulated by S-100 (tentatively referred to as protein kinase X) was partially purified from pig brain extracts. The activity of protein kinase X, which was independent of Ca2+, was demonstrated when protamine (free base), but not protamine sulfate and other proteins (including histone), was used ...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Qi DF,Turner RS,Kuo JF

    更新日期:1984-02-01 00:00:00

  • Synaptic scaffolding molecule interacts with axin.

    abstract::Synaptic scaffolding molecule (S-SCAM) is a synaptic protein that consists of PDZ domains, a guanylate kinase domain, and WW domains. It interacts with N-methyl-d-aspartate receptor subunits, neuroligin, and beta-catenin. Here, we identified Axin as a novel binding partner of S-SCAM. Axin was co-immunoprecipitated wit...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Hirabayashi S,Nishimura W,Iida J,Kansaku A,Kishida S,Kikuchi A,Tanaka N,Hata Y

    更新日期:2004-07-01 00:00:00

  • Involvement of metabotropic glutamate receptor 5 in the inhibition of methamphetamine-associated contextual memory after prolonged extinction training.

    abstract::Addiction is thought to be a memory process between perception and environmental cues and addicted patients often relapse when they come into contact with the drug-related context once again. Here, we used a conditioned place preference protocol to seek a more effective extinction methodology of methamphetamine (METH)...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Huang CH,Yu YJ,Chang CH,Gean PW

    更新日期:2016-04-01 00:00:00

  • Amyloid beta peptide 1-40 enhances the action of Toll-like receptor-2 and -4 agonists but antagonizes Toll-like receptor-9-induced inflammation in primary mouse microglial cell cultures.

    abstract::The interaction of endogenous and exogenous stimulators of innate immunity was examined in primary cultures of mouse microglial cells and macrophages after application of defined Toll-like receptor (TLR) agonists [lipopolysaccharide (LPS) (TLR4), the synthetic lipopeptide Pam3Cys-Ser-Lys4 (Pam3Cys) (TLR2) and single-s...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Lotz M,Ebert S,Esselmann H,Iliev AI,Prinz M,Wiazewicz N,Wiltfang J,Gerber J,Nau R

    更新日期:2005-07-01 00:00:00

  • A rapid anterograde axonal transport of carboxypeptidase H in rat sciatic nerves.

    abstract::Using the highly sensitive HPLC-fluorophotometry technique, anterograde and retrograde axonal transport of carboxypeptidase H (CPH), a putative prohormone processing enzyme that removes a basic amino acid from the C-terminus of a precursor peptide, was measured 12-72 h after double ligations of rat sciatic nerves. CPH...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Yajima R,Chikuma T,Kato T

    更新日期:1994-09-01 00:00:00

  • Kainate-induced apoptosis in cultured murine cerebellar granule cells elevates expression of the cell cycle gene cyclin D1.

    abstract::Recent evidence suggests that neuronal apoptosis is the consequence of an inappropriate reentry into the cell cycle. Expression of the cell cycle gene cyclin D1, a G1-phase cell cycle regulator, was examined in primary cultures of murine cerebellar granule cells (CGCs) during kainate (KA)-mediated apoptosis. Using cul...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Giardina SF,Cheung NS,Reid MT,Beart PM

    更新日期:1998-09-01 00:00:00