当前位置： SCI文献检索 > PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA期刊下所有文献 > Engineered antibody fragments for immuno-PET imaging of endogenous CD8+ T cells in vivo.

Engineered antibody fragments for immuno-PET imaging of endogenous CD8+ T cells in vivo.

Abstract：

:The noninvasive detection and quantification of CD8(+) T cells in vivo are important for both the detection and staging of CD8(+) lymphomas and for the monitoring of successful cancer immunotherapies, such as adoptive cell transfer and antibody-based immunotherapeutics. Here, antibody fragments are constructed to target murine CD8 to obtain rapid, high-contrast immuno-positron emission tomography (immuno-PET) images for the detection of CD8 expression in vivo. The variable regions of two anti-murine CD8-depleting antibodies (clones 2.43 and YTS169.4.2.1) were sequenced and reformatted into minibody (Mb) fragments (scFv-CH3). After production and purification, the Mbs retained their antigen specificity and bound primary CD8(+) T cells from the thymus, spleen, lymph nodes, and peripheral blood. Importantly, engineering of the parental antibodies into Mbs abolished the ability to deplete CD8(+) T cells in vivo. The Mbs were subsequently conjugated to S-2-(4-isothiocyanatobenzyl)-1,4,7-triazacyclononane-1,4,7-triacetic acid for (64)Cu radiolabeling. The radiotracers were injected i.v. into antigen-positive, antigen-negative, immunodeficient, antigen-blocked, and antigen-depleted mice to evaluate specificity of uptake in lymphoid tissues by immuno-PET imaging and ex vivo biodistribution. Both (64)Cu-radiolabeled Mbs produced high-contrast immuno-PET images 4 h postinjection and showed specific uptake in the spleen and lymph nodes of antigen-positive mice.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Tavaré R,McCracken MN,Zettlitz KA,Knowles SM,Salazar FB,Olafsen T,Witte ON,Wu AM

doi

10.1073/pnas.1316922111

subject

Has Abstract

pub_date

2014-01-21 00:00:00

pages

1108-13

issue

3

eissn

0027-8424

issn

1091-6490

pii

1316922111

journal_volume

111

pub_type

杂志文章

相关文献

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA文献大全
  • Deletion of a dehydratase important for intracellular growth and cording renders rough Mycobacterium abscessus avirulent.

    abstract：:Mycobacterium abscessus (Mabs) is a rapidly growing Mycobacterium and an emerging pathogen in humans. Transitioning from a smooth (S) high-glycopeptidolipid (GPL) producer to a rough (R) low-GPL producer is associated with increased virulence in zebrafish, which involves the formation of massive serpentine cords, absc...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1605477113

    authors： Halloum I,Carrère-Kremer S,Blaise M,Viljoen A,Bernut A,Le Moigne V,Vilchèze C,Guérardel Y,Lutfalla G,Herrmann JL,Jacobs WR Jr,Kremer L

    更新日期：2016-07-19 00:00:00

  • A postgermination developmental arrest checkpoint is mediated by abscisic acid and requires the ABI5 transcription factor in Arabidopsis.

    abstract：:Seed dormancy is a trait of considerable adaptive significance because it maximizes seedling survival by preventing premature germination under unfavorable conditions. Understanding how seeds break dormancy and initiate growth is also of great agricultural and biotechnological interest. Abscisic acid (ABA) plays prima...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.081594298

    authors： Lopez-Molina L,Mongrand S,Chua NH

    更新日期：2001-04-10 00:00:00

  • Isolation and characterization of delta-subspecies of protein kinase C from rat brain.

    abstract：:The delta-subspecies of protein kinase C (delta PKC) was purified to near homogeneity from the Triton X-100 extract of the rat brain particulate fraction by successive chromatographies on S-Sepharose fast flow, phenyl 5PW, heparin 5PW, hydroxyapatite, and Mono Q columns. The purified enzyme was a doublet with molecula...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.89.5.1592

    authors： Ogita K,Miyamoto S,Yamaguchi K,Koide H,Fujisawa N,Kikkawa U,Sahara S,Fukami Y,Nishizuka Y

    更新日期：1992-03-01 00:00:00

  • Ubiquitin-dependent mechanism regulates rapid turnover of AU-rich cytokine mRNAs.

    abstract：:An AU rich element (ARE) in the 3' noncoding region promotes the rapid degradation of mammalian cytokine and proto-oncogene mRNAs, such as tumor necrosis factor-alpha, granulocyte-macrophage colony-stimulating factor (GM-CSF) and c-fos. Destabilization of ARE-mRNAs involves the association of ARE-binding proteins tris...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.042575699

    authors： Laroia G,Sarkar B,Schneider RJ

    更新日期：2002-02-19 00:00:00

  • Regulation of fibrinogen synthesis by plasmin-derived fragments of fibrinogen and fibrin: an indirect feedback pathway.

    abstract：:The effect of plasmin-derived fibrinogen fragments on the biosynthesis of fibrinogen was investigated in cultured monolayers of rat hepatocytes. Incubating the cells with several concentrations of either fibrinogen or fibrin fragment D or E had no effect on the synthesis and secretion of fibrinogen by these cells. How...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.79.5.1530

    authors： Ritchie DG,Levy BA,Adams MA,Fuller GM

    更新日期：1982-03-01 00:00:00

  • Butyrate infusions in the ovine fetus delay the biologic clock for globin gene switching.

    abstract：:The switch from fetal to adult hemoglobin expression is regulated in many mammalian species by a developmental clock-like mechanism and determined by the gestational age of the fetus. Prolonging fetal globin gene expression is of considerable interest for therapeutic potential in diseases caused by abnormal beta-globi...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.85.22.8540

    authors： Perrine SP,Rudolph A,Faller DV,Roman C,Cohen RA,Chen SJ,Kan YW

    更新日期：1988-11-01 00:00:00

  • Identification of proteins that bind tightly to pre-mRNA during in vitro splicing.

    abstract：:Incubation of a human beta-globin pre-mRNA in a HeLa cell nuclear extract under conditions permissive for efficient splicing resulted in the assembly of the RNA into ribonucleoprotein (RNP) complexes. This RNP formation occurred largely within the characteristic lag period that precedes splicing. Two classes of RNP we...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.83.11.3718

    authors： Mayrand SH,Pedersen N,Pederson T

    更新日期：1986-06-01 00:00:00

  • Arsenic-induced PML targeting onto nuclear bodies: implications for the treatment of acute promyelocytic leukemia.

    abstract：:Acute promyelocytic leukemia (APL) is associated with the t(15;17) translocation, which generates a PML/RAR alpha fusion protein between PML, a growth suppressor localized on nuclear matrix-associated bodies, and RAR alpha, a nuclear receptor for retinoic acid (RA). PML/RAR alpha was proposed to block myeloid differen...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.94.8.3978

    authors： Zhu J,Koken MH,Quignon F,Chelbi-Alix MK,Degos L,Wang ZY,Chen Z,de Thé H

    更新日期：1997-04-15 00:00:00

  • Role of two of the influenza virus core P proteins in recognizing cap 1 structures (m7GpppNm) on RNAs and in initiating viral RNA transcription.

    abstract：:Purified influenza viral cores catalyze the entire process of viral RNA transcription, which includes the endonucleolytic cleavage of heterologous RNAs containing cap 1 (m(7)GpppNm) structures to generate capped primers 10-13 nucleotides long, the initiation of transcription via the incorporation of a guanosine residu...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.78.12.7355

    authors： Ulmanen I,Broni BA,Krug RM

    更新日期：1981-12-01 00:00:00

  • Ethylene-insensitive tobacco lacks nonhost resistance against soil-borne fungi.

    abstract：:Enhanced ethylene production is an early response of plants to pathogen attack and has been associated with both resistance and susceptibility to disease. Tobacco plants were transformed with the mutant etr1-1 gene from Arabidopsis, conferring dominant ethylene insensitivity. Besides lacking known ethylene responses, ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.95.4.1933

    authors： Knoester M,van Loon LC,van den Heuvel J,Hennig J,Bol JF,Linthorst HJ

    更新日期：1998-02-17 00:00:00

  • Increased expression of the putative growth factor receptor p185HER2 causes transformation and tumorigenesis of NIH 3T3 cells.

    abstract：:The HER2 gene encodes a cell-surface glycoprotein with extensive homology to the epidermal growth factor receptor. Recently it was found to be amplified in about 30% of primary human breast malignancies. In experiments designed to assess the role of the HER2 gene in oncogenesis, we found that overexpression of unalter...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.84.20.7159

    authors： Hudziak RM,Schlessinger J,Ullrich A

    更新日期：1987-10-01 00:00:00

  • Asymmetric phosphorylation through catalytic P(III) phosphoramidite transfer: enantioselective synthesis of D-myo-inositol-6-phosphate.

    abstract：:Despite the ubiquitous use of phosphoramidite chemistry in the synthesis of biophosphates, catalytic asymmetric phosphoramidite transfer remains largely unexplored for phosphate ester synthesis. We have discovered that a tetrazole-functionalized peptide, in the presence of 10-Å molecular sieves, functions as an enanti...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1001111107

    authors： Jordan PA,Kayser-Bricker KJ,Miller SJ

    更新日期：2010-11-30 00:00:00

  • Symmetries throughout organic evolution.

    abstract：:The biological realm has inherited symmetries from the physicochemical realm, but with the increasing complexity at higher phenomenological levels of life, some inherited symmetries are broken while novel symmetries appear. These symmetries are of two types, structural and operational. Biological novelties result from...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.93.25.14229

    authors： García-Bellido A

    更新日期：1996-12-10 00:00:00

  • Regulation of zinc homeostasis by inducible NO synthase-derived NO: nuclear metallothionein translocation and intranuclear Zn2+ release.

    abstract：:Zn2+ is critical for the functional and structural integrity of cells and contributes to a number of important processes including gene expression. It has been shown that NO exogenously applied via NO donors resulting in nitrosative stress leads to cytoplasmic Zn2+ release from the zinc storing protein metallothionein...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.2335190100

    authors： Spahl DU,Berendji-Grün D,Suschek CV,Kolb-Bachofen V,Kröncke KD

    更新日期：2003-11-25 00:00:00

  • Affinity-purified interleukin 2 induces proliferation of large but not small B cells.

    abstract：:Immunoaffinity-purified interleukin 2 (IL2) stimulated proliferation of large but not small B cells. Stimulation was observed even when B cells were cultured at very low cell densities (3 X 10(4) per microwell containing 0.2 ml of medium). Addition of small numbers of purified splenic T cells did not enhance the IL2-i...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.82.5.1518

    authors： Mond JJ,Thompson C,Finkelman FD,Farrar J,Schaefer M,Robb RJ

    更新日期：1985-03-01 00:00:00

  • Calmodulin priming: nuclear translocation of a calmodulin complex and the memory of prior neuronal activity.

    abstract：:The neuronal nucleus plays a vital role in information processing, but whether it supports computational functions such as paired-pulse facilitation, comparable to synapses, is unclear. Ca(2+)-dependent movement of calmodulin (CaM) to the nucleus is highly responsive to Ca(2+) entry through L-type channels and promote...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.211563998

    authors： Mermelstein PG,Deisseroth K,Dasgupta N,Isaksen AL,Tsien RW

    更新日期：2001-12-18 00:00:00

  • Targeted expression of heme oxygenase-1 prevents the pulmonary inflammatory and vascular responses to hypoxia.

    abstract：:Chronic hypoxia causes pulmonary hypertension with smooth muscle cell proliferation and matrix deposition in the wall of the pulmonary arterioles. We demonstrate here that hypoxia also induces a pronounced inflammation in the lung before the structural changes of the vessel wall. The proinflammatory action of hypoxia ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.161272598

    authors： Minamino T,Christou H,Hsieh CM,Liu Y,Dhawan V,Abraham NG,Perrella MA,Mitsialis SA,Kourembanas S

    更新日期：2001-07-17 00:00:00

  • Effect of uncouplers on radiosensitivity and mutagenicity in x-irradiated mammalian cells.

    abstract：:The number of x-irradiated mammalian cells surviving is markedly increased when the cells are incubated with an uncoupler of oxidative phosphorylation prior to or immediately after irradiation. This increase is greater in plateau-phase cells than in exponentially growing cells. The increase in survival is related to t...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.77.5.2702

    authors： Laval F

    更新日期：1980-05-01 00:00:00

  • The ribosome-in-pieces: binding of elongation factor EF-G to oligoribonucleotides that mimic the sarcin/ricin and thiostrepton domains of 23S ribosomal RNA.

    abstract：:An oligoribonucleotide (a 27-mer) that mimics the sarcin/ricin (S/R) domain of Escherichia coli 23S rRNA binds elongation factor EF-G; the Kd is 6.9 microM, whereas for binding to ribosomes it is 0.7 microM. Binding saturates when EF-G and the S/R RNA are equimolar; at saturation 70% of the input RNA is in complexes w...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.94.23.12280

    authors： Munishkin A,Wool IG

    更新日期：1997-11-11 00:00:00

  • Parasite stress and pathogen avoidance relate to distinct dimensions of political ideology across 30 nations.

    abstract：:People who are more avoidant of pathogens are more politically conservative, as are nations with greater parasite stress. In the current research, we test two prominent hypotheses that have been proposed as explanations for these relationships. The first, which is an intragroup account, holds that these relationships ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章,多中心研究

    doi：10.1073/pnas.1607398113

    authors： Tybur JM,Inbar Y,Aarøe L,Barclay P,Barlow FK,de Barra M,Becker DV,Borovoi L,Choi I,Choi JA,Consedine NS,Conway A,Conway JR,Conway P,Adoric VC,Demirci DE,Fernández AM,Ferreira DC,Ishii K,Jakšić I,Ji T,van Leeuwen

    更新日期：2016-11-01 00:00:00

  • The genomic basis of trophic strategy in marine bacteria.

    abstract：:Many marine bacteria have evolved to grow optimally at either high (copiotrophic) or low (oligotrophic) nutrient concentrations, enabling different species to colonize distinct trophic habitats in the oceans. Here, we compare the genome sequences of two bacteria, Photobacterium angustum S14 and Sphingopyxis alaskensis...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0903507106

    authors： Lauro FM,McDougald D,Thomas T,Williams TJ,Egan S,Rice S,DeMaere MZ,Ting L,Ertan H,Johnson J,Ferriera S,Lapidus A,Anderson I,Kyrpides N,Munk AC,Detter C,Han CS,Brown MV,Robb FT,Kjelleberg S,Cavicchioli R

    更新日期：2009-09-15 00:00:00

  • Activation of the polyomavirus enhancer by a murine activator protein 1 (AP1) homolog and two contiguous proteins.

    abstract：:The polyomavirus enhancer is composed of multiple DNA sequence elements serving as binding sites for proteins present in mouse nuclear extracts that activate transcription and DNA replication. We have identified three such proteins and their binding sites and correlate them with enhancer function. Mutation of nucleoti...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.85.16.5839

    authors： Martin ME,Piette J,Yaniv M,Tang WJ,Folk WR

    更新日期：1988-08-01 00:00:00

  • Reduced fertility in mice deficient for the POU protein sperm-1.

    abstract：:Members of the POU-homeodomain gene family encode transcriptional regulatory molecules that play important roles in terminal differentiation of many organ systems. Sperm-1 (Sprm-1) is a POU domain factor that is exclusively expressed in the differentiating male germ cell. We show here that the Sprm-1 protein is expres...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.94.14.7555

    authors： Pearse RV 2nd,Drolet DW,Kalla KA,Hooshmand F,Bermingham JR Jr,Rosenfeld MG

    更新日期：1997-07-08 00:00:00

  • Gel-forming mucins form distinct morphologic structures in airways.

    abstract：:Gel-forming mucins, the primary macromolecular components of airway mucus, facilitate airway clearance by mucociliary transport. In cystic fibrosis (CF) altered mucus properties impair mucociliary transport. Airways primarily secrete two closely related gel-forming mucins, MUC5B and MUC5AC. However, their morphologic ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1703228114

    authors： Ostedgaard LS,Moninger TO,McMenimen JD,Sawin NM,Parker CP,Thornell IM,Powers LS,Gansemer ND,Bouzek DC,Cook DP,Meyerholz DK,Abou Alaiwa MH,Stoltz DA,Welsh MJ

    更新日期：2017-06-27 00:00:00

  • Larval helpers and age polyethism in ambrosia beetles.

    abstract：:Division of labor among the workers of insect societies is a conspicuous feature of their biology. Social tasks are commonly shared among age groups but not between larvae and adults with completely different morphologies, as in bees, wasps, ants, and beetles (i.e., Holometabola). A unique yet hardly studied holometab...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1107758108

    authors： Biedermann PH,Taborsky M

    更新日期：2011-10-11 00:00:00

  • Pituitary growth hormone network responses are sexually dimorphic and regulated by gonadal steroids in adulthood.

    abstract：:There are well-recognized sex differences in many pituitary endocrine axes, usually thought to be generated by gonadal steroid imprinting of the neuroendocrine hypothalamus. However, the recognition that growth hormone (GH) cells are arranged in functionally organized networks raises the possibility that the responses...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1010849107

    authors： Sanchez-Cardenas C,Fontanaud P,He Z,Lafont C,Meunier AC,Schaeffer M,Carmignac D,Molino F,Coutry N,Bonnefont X,Gouty-Colomer LA,Gavois E,Hodson DJ,Le Tissier P,Robinson IC,Mollard P

    更新日期：2010-12-14 00:00:00

  • Antibody targeting studies in a transgenic murine model of spontaneous colorectal tumors.

    abstract：:Monoclonal antibodies (mAbs) have been used to treat malignancies in humans with varying degrees of success. Progress has been hindered by the lack of suitable animal models, which would ideally consist of immunocompetent animals that are tolerant to tumor-associated antigens. Suitable models would allow the study and...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.181353498

    authors： Wilkinson RW,Ross EL,Poulsom R,Ilyas M,Straub J,Snary D,Bodmer WF,Mather SJ

    更新日期：2001-08-28 00:00:00

  • Recurrent axon collaterals underlie facilitating synapses between cerebellar Purkinje cells.

    abstract：:Morphological studies have provided ample evidence for synaptic connections between cerebellar Purkinje cells (PCs), but the functional properties of these synapses remain elusive. We report on direct recordings of synaptically connected PCs in mice cerebellar slices. In PCs filled with a fluorescent dye to aid axon v...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0707489104

    authors： Orduz D,Llano I

    更新日期：2007-11-06 00:00:00

  • Self-organization into ferroelectric and antiferroelectric crystals via the interplay between particle shape and dipolar interaction.

    abstract：:Ferroelectricity and antiferroelectricity are widely seen in various types of condensed matter and are of technological significance not only due to their electrical switchability but also due to intriguing cross-coupling effects such as electro-mechanical and electro-caloric effects. The control of the two types of d...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1809004115

    authors： Takae K,Tanaka H

    更新日期：2018-10-02 00:00:00

  • Higher-order syzygies for the bracket algebra and for the ring of coordinates of the Grassmanian.

    abstract：:A Poincaré resolution is given for the supersymmetric ring of brackets over a signed alphabet. As a consequence, a resolution is found for the ring of coordinates of the Grassmanian variety in projective space over any infinite field. ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.88.18.8087

    authors： Anick D,Rota GC

    更新日期：1991-09-15 00:00:00