Self-organization into ferroelectric and antiferroelectric crystals via the interplay between particle shape and dipolar interaction.

abstract：:With current concerns of antibiotic-resistant bacteria and biodefense, it has become important to rapidly identify infectious bacteria. Traditional technologies involving isolation and amplification of the pathogenic bacteria are time-consuming. We report a rapid and simple method that combines in vivo biotinylation o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Edgar R,McKinstry M,Hwang J,Oppenheim AB,Fekete RA,Giulian G,Merril C,Nagashima K,Adhya S

更新日期：2006-03-28 00:00:00

abstract：:Lymphangiogenesis plays an important role in promoting cancer metastasis to sentinel lymph nodes and beyond and also promotes organ transplant rejection. We used human lymphatic endothelial cells to establish a reliable three-dimensional lymphangiogenic sprouting assay with automated image acquisition and analysis for...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schulz MM,Reisen F,Zgraggen S,Fischer S,Yuen D,Kang GJ,Chen L,Schneider G,Detmar M

更新日期：2012-10-02 00:00:00

abstract：:The motility of cilia and flagella is powered by dynein ATPases associated with outer doublet microtubules. However, a flagellar kinesin-like protein that may function as a motor associates with the central pair complex. We determined that Chlamydomonas reinhardtii central pair kinesin Klp1 is a phosphoprotein and, li...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yokoyama R,O'toole E,Ghosh S,Mitchell DR

更新日期：2004-12-14 00:00:00

abstract：:Chronic, low grade, sterile inflammation frequently accompanies aging and age-related diseases. Cellular senescence is associated with the production of proinflammatory chemokines, cytokines, and extracellular matrix (ECM) remodeling proteases, which comprise the senescence-associated secretory phenotype (SASP). We fo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xu M,Tchkonia T,Ding H,Ogrodnik M,Lubbers ER,Pirtskhalava T,White TA,Johnson KO,Stout MB,Mezera V,Giorgadze N,Jensen MD,LeBrasseur NK,Kirkland JL

更新日期：2015-11-17 00:00:00

abstract：:Antibodies prepared against peptides CP2, CP4, and CP5, which occur within the first 1522 amino acid residues of the alpha 1 subunit of dihydropyridine-sensitive skeletal muscle calcium channels, specifically recognized a 175-kDa form of the alpha 1 subunit in immunoblots and immunoprecipitation experiments. In contra...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： De Jongh KS,Merrick DK,Catterall WA

更新日期：1989-11-01 00:00:00

abstract：:Shell shape and shell thickness of the intertidal snail Littorina obtusata changed markedly between 1871 and 1984 in northern New England. Shells collected prior to 1900 were high-spired with thin walls, whereas shells collected in 1982-84 were low-spired with thick walls. An intertidal crab (Carcinus maenas) which pr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Seeley RH

更新日期：1986-09-01 00:00:00

abstract：:The knowledge of tropical palaeoclimates is crucial for understanding global climate change, because it is a test bench for general circulation models that are ultimately used to predict future global warming. A longstanding issue concerning the last glacial maximum in the tropics is the discrepancy between the decrea...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wu H,Guiot J,Brewer S,Guo Z,Peng C

更新日期：2007-06-05 00:00:00

abstract：:Three proteins required for poly(U)-directed polyphenylalanine synthesis have been separated from yeast. Two of the factors correspond to the elongation factors 1 and 2 described for other eukaryotic systems, according to the criteria of phenylalanyl-tRNA binding and diphtheria toxin-catalyzed ADP-ribosylation. The th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Skogerson L,Wakatama E

更新日期：1976-01-01 00:00:00

abstract：:The high resolution 1H and 31P NMR spectra of the (dG-dC)8 duplex have been recorded in low- and high-salt solutions in order to evaluate the structural aspects of the salt-induced transition of oligo(dG-dC) in solution [Pohl, F. M. & Jovin, T. M. (1972) J. Mol. Biol. 67, 375-396]. The NMR data require that the (dG-d...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Patel DJ,Canuel LL,Pohl FM

更新日期：1979-06-01 00:00:00

abstract：:In Escherichia coli, AK I- HDH I and AK II- HDH II are two bifunctional proteins, derived from a common ancestor, that catalyze the first and third reactions of the common pathway leading to threonine and methionine. An extensive amino acid sequence comparison of both molecules reveals two main features on each of the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ferrara P,Duchange N,Zakin MM,Cohen GN

更新日期：1984-05-01 00:00:00

abstract：:An intense (up to 20 muA/cm(2)) steady electrical current enters the anterior or nurse cell end of the growing follicle (or oocyte-nurse cell complex) of the Cecropia moth and is balanced by a more diffuse current leaving elsewhere. In late growth stages, the total transfollicular current is about 100 nA. Moreover, a ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jaffe LF,Woodruff RI

更新日期：1979-03-01 00:00:00

abstract：:Candidate single-nucleotide polymorphisms (SNPs) were analyzed for associations to an unselected whole genome pool of tumor mRNA transcripts in 50 unrelated patients with breast cancer. SNPs were selected from 203 candidate genes of the reactive oxygen species pathway. We describe a general statistical framework for t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kristensen VN,Edvardsen H,Tsalenko A,Nordgard SH,Sørlie T,Sharan R,Vailaya A,Ben-Dor A,Lønning PE,Lien S,Omholt S,Syvänen AC,Yakhini Z,Børresen-Dale AL

更新日期：2006-05-16 00:00:00

abstract：:The postdevelopmental basis of cellular identity and the unique cellular output of a particular neuron type are of particular interest in the nervous system because a detailed understanding of circuits responsible for complex processes in the brain is impeded by the often ambiguous classification of neurons in these c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schulz DJ,Goaillard JM,Marder EE

更新日期：2007-08-07 00:00:00

abstract：:Alfalfa mosaic virus (AlMV) coat protein is involved in systemic infection of host plants, and a specific mutation in this gene prevents the virus from moving into the upper uninoculated leaves. The coat protein also is required for different viral functions during early and late infection. To study the role of the co...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Spitsin S,Steplewski K,Fleysh N,Belanger H,Mikheeva T,Shivprasad S,Dawson W,Koprowski H,Yusibov V

更新日期：1999-03-02 00:00:00

abstract：:We measured quantitatively the binding affinities of purified Cro repressor to the chemically synthesized wild-type and mutant OR1 operators, consisting of all three possible base-pair substitutions and of thymine to uracil substitutions at each base-pair position of the 17-base-pair operator sequence. The sequence-sp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Takeda Y,Sarai A,Rivera VM

更新日期：1989-01-01 00:00:00

abstract：:The effective management of marine fisheries is an ongoing challenge at the intersection of biology, economics, and policy. One way in which fish stocks-and their habitats-can be protected is through the establishment of marine reserves, areas that are closed to fishing. Although the potential economic benefits of suc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Herrera GE,Moeller HV,Neubert MG

更新日期：2016-04-05 00:00:00

abstract：:We performed the biochemical and biophysical characterization of a red fluorescent protein, eqFP611, from the sea anemone Entacmaea quadricolor cloned in Escherichia coli. With an excitation maximum at 559 nm and an emission maximum at 611 nm, the recombinant protein shows the most red-shifted emission and the largest...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wiedenmann J,Schenk A,Röcker C,Girod A,Spindler KD,Nienhaus GU

更新日期：2002-09-03 00:00:00

abstract：:Expression of glucokinase in hepatocytes and pancreatic 6-cells is of major physiologic importance to mammalian glucose homeostasis. Liver glucokinase catalyzes the first committed step in the disposal of glucose, and beta-cell glucokinase catalyzes a rate-limiting step required for glucose-regulated insulin release. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Heimberg H,De Vos A,Moens K,Quartier E,Bouwens L,Pipeleers D,Van Schaftingen E,Madsen O,Schuit F

更新日期：1996-07-09 00:00:00

abstract：:A theoretical description of aqueous hydration in the minor groove of a B-form DNA is presented on the basis of a liquid-state Monte Carlo computer simulation on a system consisting of the oligonucleotide duplex d(CGCGAATTCGCG).d(GCGCTTAAGCGC) in a canonical B-form together with 1777 water molecules contained in a hex...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Subramanian PS,Ravishanker G,Beveridge DL

更新日期：1988-03-01 00:00:00

abstract：:DNA heteroduplexes with single unpaired bases of the four different kinds were prepared by annealing separated strands of bacteriophage lambda DNA and used to transfect Escherichia coli. Genetic analysis of the progeny phages obtained from transfected bacteria indicates that the E. coli mismatch repair system can reco...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dohet C,Wagner R,Radman M

更新日期：1986-05-01 00:00:00

abstract：:The "parallel-up" packing in cellulose Ialpha and Ibeta unit cells was experimentally demonstrated by a combination of direct-staining the reducing ends of cellulose chains and microdiffraction-tilting electron crystallographic analysis. Microdiffraction investigation of nascent bacterial cellulose microfibrils showed...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Koyama M,Helbert W,Imai T,Sugiyama J,Henrissat B

更新日期：1997-08-19 00:00:00

abstract：:It is generally believed that serial passage at high multiplicity of infection (moi) is required for the generation of defective interfering (DI) particles. High levels of DI RNAs are found associated with persistent infections initiated with laboratory cultures of cucumber necrosis virus (CNV). Two synthetic CNV tran...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rochon DM

更新日期：1991-12-15 00:00:00

abstract：:The gaseous phytohormone ethylene C(2)H(4) mediates numerous aspects of growth and development. Genetic analysis has identified a number of critical elements in ethylene signaling, but how these elements interact biochemically to transduce the signal from the ethylene receptor complex at the endoplasmic reticulum (ER)...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ju C,Yoon GM,Shemansky JM,Lin DY,Ying ZI,Chang J,Garrett WM,Kessenbrock M,Groth G,Tucker ML,Cooper B,Kieber JJ,Chang C

更新日期：2012-11-20 00:00:00

abstract：:An increase in free Ca2+ triggers exocytosis in pituitary nerve terminals leading to an increase in membrane area and membrane capacitance. When Ca2+ is increased by step depolarization, an instantaneous capacitance increase during the first 80 ms is followed by a slow increase extending over several seconds. We measu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rosenboom H,Lindau M

更新日期：1994-06-07 00:00:00

abstract：:Thymic epithelial cells (TECs), the major component of the thymic microenvironment, can be modulated by pituitary hormones. We have shown previously that prolactin (PRL) can influence the endocrine activity of TECs and stimulate TEC proliferation as well as cytokeratin expression, suggesting the existence of PRL recep...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dardenne M,Kelly PA,Bach JF,Savino W

更新日期：1991-11-01 00:00:00

abstract：:Oxidation of genomic DNA forms the guanine lesion 7,8-dihydro-8-oxoguanine (8-oxoG). When in the template base position during DNA synthesis the 8-oxoG lesion has dual coding potential by virtue of its anti- and syn-conformations, base pairing with cytosine and adenine, respectively. This impacts mutagenesis, because ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Batra VK,Shock DD,Beard WA,McKenna CE,Wilson SH

更新日期：2012-01-03 00:00:00

abstract：:Protein misfolding and deposition underlie an increasing number of debilitating human disorders. We have shown that model proteins unrelated to disease, such as the Src homology 3 (SH3) domain of the p58alpha subunit of bovine phosphatidyl-inositol-3'-kinase (PI3-SH3), can be converted in vitro into assemblies with st...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ventura S,Zurdo J,Narayanan S,Parreño M,Mangues R,Reif B,Chiti F,Giannoni E,Dobson CM,Aviles FX,Serrano L

更新日期：2004-05-11 00:00:00

abstract：:Conjugation of drugs with antibodies to surface endothelial antigens is a potential strategy for drug delivery to endothelium. We studied antibodies to platelet-endothelial adhesion molecule 1 (PECAM-1, a stably expressed endothelial antigen) as carriers for vascular immunotargeting. Although 125I-labeled anti-PECAM b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Muzykantov VR,Christofidou-Solomidou M,Balyasnikova I,Harshaw DW,Schultz L,Fisher AB,Albelda SM

更新日期：1999-03-02 00:00:00

abstract：:The Skp1-Cullin1 F-box protein-Fbw7 ubiquitin ligase regulates phosphorylation-dependent cyclin E degradation, and disruption of this pathway is associated with genetic instability and tumorigenesis. Fbw7 is a human tumor suppressor that is targeted for mutation in primary cancers. However, mechanisms other than mutat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Minella AC,Welcker M,Clurman BE

更新日期：2005-07-05 00:00:00

abstract：:We have developed a procedure for purifying highly specific polyclonal antibodies against 5-methylcytosine. These antibodies were used to probe the distribution of 5-methylcytosine among fractionated nucleosomes of mouse cell chromatin. Our results demonstrate that at least 80% of the 5-methylcytosine is localized in ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ball DJ,Gross DS,Garrard WT

更新日期：1983-09-01 00:00:00