A mechanistic study on the metastasis inducing function of FUS-CHOP fusion protein in liposarcoma.


:The FUS-CHOP fusion protein has been found to be instrumental for specific oncogenic processes in liposarcoma, but its ability to induce metastasis and the underlying mechanisms by which this can be achieved remain unknown. To dissect its functional role in this context, we stably overexpressed this protein in SW872 liposarcoma and HT1080 fibrosarcoma cell lines, and were able to demonstrate that forced expression of FUS-CHOP significantly increases migration and invasion, as well as enhances lung and liver metastasis in the in vivo chicken chorioallantoic membrane (CAM) model, that is proliferation independent. Additionally, FUS-CHOP enhances the expression of matrix-metalloproteinases -2 and -9, and transactivates their promoters in vitro. Mutational analysis showed that C/EBP-β- (-769/-755), NF-κB (-525/-516) and CREB/AP-1 (-218/-207) sites were important for MMP-2 and NF-κB (-604/-598), AP-1 (-539/-532) and AP-1 (-81/-72) for MMP-9 transactivation. Moreover, a direct in vivo interaction of FUS-CHOP was observed in case of the MMP-2 promoter within region (-769/-207). siRNA data revealed that MMP-2 expression is essential in the FUS-CHOP induced metastatic phenotype. MMP-2-mRNA and protein expression correlated significantly with FUS-CHOP positivity in 46 resected patient liposarcoma tissues. We have for the first time provided substantial evidence for the FUS-CHOP oncoprotein as an inducer of metastasis that is due to the transcriptional induction of specific tumor-associated proteases. Insights gained from this study not only support a deeper understanding of the mechanistic properties of FUS-CHOP, but also open up new avenues for targeted therapy.


Int J Cancer


Patil N,Ahmed Kabeer Rasheed S,Abba M,Hendrik Leupold J,Schwarzbach M,Allgayer H




Has Abstract


2014-06-15 00:00:00












  • Possible contribution of active MMP2 to lymph-node metastasis and secreted cathepsin L to bone invasion of newly established human oral-squamous-cancer cell lines.

    abstract::We have established human oral-squamous-cancer cell lines, BHY and HN, derived from non-metastatic cancer and metastatic cancer respectively. We examined the expression of matrix-degrading enzymes and their inhibitors in these cell lines. Both cell lines expressed pro-matrix metalloproteinase (MMP)1, proMMP2, proMMP9,...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Kawamata H,Nakashiro K,Uchida D,Harada K,Yoshida H,Sato M

    更新日期:1997-01-06 00:00:00

  • Potentiation of the anti-tumor effect of actinomycin D by tumor necrosis factor alpha in mice: correlation between in vitro and in vivo results.

    abstract::The anti-tumor effects of actinomycin D (Act D) and recombinant human tumor necrosis factor (TNF)-alpha have been studied on 4 established murine tumor cell lines: MmB16 melanoma, Lewis lung (LL/2) carcinoma, L1 sarcoma and L1210 leukemia. During short-term incubation (24 hr) Act D produced dose-dependent cytostatic/c...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Lasek W,Giermasz A,Kuc K,Wańkowicz A,Feleszko W,Gołab J,Zagozdzon R,Stokłosa T,Jakóbisiak M

    更新日期:1996-05-03 00:00:00

  • Poor oral health is associated with an increased risk of esophageal squamous cell carcinoma - a population-based case-control study in China.

    abstract::To further examine the association between oral hygiene and esophageal squamous cell carcinoma (ESCC) risk and the effect modification of other exposures, we conducted a population-based case-control study between 2010 and 2012 in Taixing, China, a high-risk area for ESCC. Cases were primarily recruited from endoscopy...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Chen X,Yuan Z,Lu M,Zhang Y,Jin L,Ye W

    更新日期:2017-02-01 00:00:00

  • Induction of plasma membrane alterations in AKR-2B mouse embryo fibroblasts by endogenous growth factors from malignant human cells.

    abstract::AKR-2B mouse fibroblasts were treated with 50 micrograms/ml of crude transforming growth factor (TGF) of human origin. Cell surface proteins of treated cells were radioiodinated and compared to untreated cells at various times after the addition of TGF. Treated cells showed a severalfold increase (approximately 6-fold...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Marks ME,Brattain MG

    更新日期:1984-01-15 00:00:00

  • Canine transmissible venereal sarcoma: leukocyte adherence inhibition (LAI) reactivity of various lymphoid tissues of dogs with tumors at different stages of growth.

    abstract::Leukocyte adherence inhibition (LAI) reactivity of various lymphoid tissues of dogs with canine transmissible venereal sarcoma (CTVS) at different stages of growth was determined by the tube LAI test. Tumors were classified at the time of excision into progressor, steady state, and regressor stages of growth. The LAI ...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Harding MW,Yang TJ

    更新日期:1981-03-15 00:00:00

  • Effects of the farnesyltransferase inhibitor UCF-1C/manumycin on growth and p21-ras post-translational processing in NIH3T3 cells.

    abstract::Examination of the effect of the farnesylprotein transferase (FPTase) inhibitor UCF1-C/manumycin on NIH3T3 cells transfected with a normal N-ras gene and expressing high levels of the corresponding p21-ras protein showed that 10 microm UCF1-C immediately and reversibly inhibited growth in these cells, without modifyin...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Servais P,Gulbis B,Fokan D,Galand P

    更新日期:1998-05-18 00:00:00

  • Prospective study of alcohol consumption and breast cancer risk in Japanese women.

    abstract::Epidemiologic evidence is lacking for the association between alcohol consumption and the risk of breast cancer in Japanese women. We addressed this association in a prospective cohort study with an average follow-up of 7.6 years. At baseline (1988-1990), cohort participants completed a self-administered questionnaire...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Lin Y,Kikuchi S,Tamakoshi K,Wakai K,Kondo T,Niwa Y,Yatsuya H,Nishio K,Suzuki S,Tokudome S,Yamamoto A,Toyoshima H,Tamakoshi A

    更新日期:2005-09-20 00:00:00

  • Cross-reactions between tumor cells and allogeneic normal tissues. Inhibition of a syngeneic lymphoma outgrowth in H-2 and non-H-2 alloimmune BALB/c mice.

    abstract::To test whether alloimmunization with H-2 or/and non-H-2 different normal tissues may increase the immunity to syngeneic tumors, groups of BALB/c (H-2d) mice were immunized with a series of allogeneic lymphoid cells and then challenged i.p. with syngeneic lymphoma cells. The outgrowth of otherwise lethal doses of the ...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Parmiani G,Sensi ML,Carbone G,Colombo M,Pierotti M,Ballinari D,Hilgers J,Hilkens J

    更新日期:1982-03-15 00:00:00

  • Mother-to-child transmission of human T-cell-leukemia/lymphoma virus type I: implication of high antiviral antibody titer and high proviral load in carrier mothers.

    abstract::In order to gain new insights into the risk factors influencing human-T-cell-leukemia/lymphoma-virus-type-I (HTLV-I) mother-to-child transmission, a retrospective study of HTLV-I infection among children born to HTLV-I-seropositive women was carried out in a highly HTLV-I-endemic population of African origin living in...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Ureta-Vidal A,Angelin-Duclos C,Tortevoye P,Murphy E,Lepère JF,Buigues RP,Jolly N,Joubert M,Carles G,Pouliquen JF,de Thé G,Moreau JP,Gessain A

    更新日期:1999-09-09 00:00:00

  • Subtypes of Epstein-Barr virus in HIV-1-associated and HIV-1-unrelated Hodgkin's disease cases.

    abstract::Epstein-Barr virus (EBV) type 2 is considered to be a much less potent transformer of lymphocytes than type 1. However, type-2 EBV may be involved in the pathogenesis of non-Hodgkin's lymphomas (NHLs) arising in immunocompromised patients, i.e., subjects with malaria or HIV-1 infection. To determine whether type-2 EBV...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: De Re V,Boiocchi M,De Vita S,Dolcetti R,Gloghini A,Uccini S,Baroni C,Scarpa A,Cattoretti G,Carbone A

    更新日期:1993-07-30 00:00:00

  • Changes in glycolipids in human renal-cell carcinoma and their clinical significance.

    abstract::The expression patterns of glycolipids of human renal-cell carcinoma were studied in primary tumors from 23 cases and 5 metastatic lesions from 4 cases using HPTLC (high-performance thin-layer chromatography). The expression pattern of glycolipids in primary tumors was characteristic of the histological cell type. In ...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Saito S,Orikasa S,Ohyama C,Satoh M,Fukushi Y

    更新日期:1991-09-30 00:00:00

  • Transient immunological and clinical effectiveness of treating mice bearing premalignant oral lesions with PD-1 antibodies.

    abstract::A carcinogen-induced premalignant oral lesion model that progresses to oral cancer was used to examine the impact of blocking PD-1 on cytokine expression and on progression of lesions to cancer. The results of this study show increased production of IL-2 and the inflammatory cytokines IL-6, IL-17 and TNF-α by spleen c...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Levingston CA,Young MR

    更新日期:2017-04-01 00:00:00

  • Correlation of total VEGF mRNA and protein expression with histologic type, tumor angiogenesis, patient survival and timing of relapse in non-small-cell lung cancer.

    abstract::We have quantified the expression of all 4 isoforms of vascular endothelial growth factor (VEGF) mRNA in non-small-cell lung cancer (NSCLC) using a new kinetic quantitative PCR method, real-time quantitative (RTQ) RT-PCR, and investigated the association between VEGF expression at the mRNA and protein levels and the c...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Yuan A,Yu CJ,Chen WJ,Lin FY,Kuo SH,Luh KT,Yang PC

    更新日期:2000-11-20 00:00:00

  • Regulation of c-fgr proto-oncogene expression in Epstein-Barr virus infected B-cell lines.

    abstract::We and others have previously shown that in vitro conversion of Epstein-Barr virus (EBV)-negative Burkitt lymphoma (BL) cell lines with the immortalizing B95-8 strain of EBV results in a marked elevation in levels of c-fgr proto-oncogene mRNA. We now show, using a nuclear run-off assay, that this induction results fro...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Patel M,Leevers SJ,Brickell PM

    更新日期:1990-02-15 00:00:00

  • Progression of chronic atrophic gastritis associated with Helicobacter pylori infection increases risk of gastric cancer.

    abstract::We conducted a longitudinal cohort study to determine the association of Helicobacter pylori infection and the progression of chronic atrophic gastritis (CAG) with gastric cancer. A cohort of 4655 healthy asymptomatic subjects was followed for a mean period of 7.7 years. H. pylori infection was established by serum sp...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Ohata H,Kitauchi S,Yoshimura N,Mugitani K,Iwane M,Nakamura H,Yoshikawa A,Yanaoka K,Arii K,Tamai H,Shimizu Y,Takeshita T,Mohara O,Ichinose M

    更新日期:2004-03-01 00:00:00

  • Bcl-2 expression and prognosis of patients with endometrial carcinoma.

    abstract::Bcl-2 protein inhibits apoptosis, reduces the requirement for growth factors, and thereby extends the survival of cells. Recent findings of Bcl-2 in several solid tumors suggest that it might contribute to the genesis of some types of cancer. Over-expression of Bcl-2 might play a role in carcinogenesis and malignant p...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Sakuragi N,Ohkouchi T,Hareyama H,Ikeda K,Watari H,Fujimoto T,Kuwabara M,Yamamoto R,Sagawa T,Fujino T,Fujimoto S

    更新日期:1998-04-17 00:00:00

  • Gene expression profiling of peripheral blood mononuclear cells during treatment with a gene-modified allogeneic tumor cell vaccine in advanced renal cell cancer: tumor-induced immunosuppression and a possible role for NF-κB.

    abstract::Tumor-induced immunosuppression remains a major challenge for immunotherapy of cancer patients. To further elucidate why an allogeneic gene-modified [interleukin-7 (IL-7)/CD80-cotransfected] renal cell cancer (RCC) vaccine failed to induce clinically relevant TH-1-polarized immune responses, peripheral blood mononucle...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Flörcken A,Grau M,Wolf A,Weilemann A,Kopp J,Dörken B,Blankenstein T,Pezzutto A,Lenz P,Lenz G,Westermann J

    更新日期:2015-04-15 00:00:00

  • Association of reduced cell adhesion regulator messenger RNA expression with tumor progression in human hepatocellular carcinoma.

    abstract::The recently identified cell adhesion regulator (CAR) modulates the process of integrin-mediated cell adhesion. The CAR gene is located on 16q, a locus at which high levels of allelic losses have been demonstrated in advanced human hepatocellular carcinoma (HCC). We studied the possible involvement of the CAR gene in ...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Yamamoto H,Itoh F,Sakamoto H,Nakajima Y,Une Y,Hinoda Y,Imai K

    更新日期:1997-06-20 00:00:00

  • Incidence of cutaneous childhood melanoma in Queensland, Australia.

    abstract::The incidence of cutaneous melanoma in children aged 0-14 years was examined in Queensland, Australia. Details of notifications were collected from the population-based Australian Paediatric Cancer Registry. Between 1987 and 1994, the age-adjusted incidence rates of invasive cutaneous melanoma occurring in Queensland ...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Whiteman D,Valery P,McWhirter W,Green A

    更新日期:1995-12-11 00:00:00

  • Several genes encoding ribosomal proteins are over-expressed in prostate-cancer cell lines: confirmation of L7a and L37 over-expression in prostate-cancer tissue samples.

    abstract::A cDNA library specific for mRNA over-expressed in prostate cancer was generated by subtractive hybridization of transcripts originating from prostatic hyperplasia and cancer tissues. cDNA encoding ribosomal proteins L4, L5, L7a, L23a, L30, L37, S14 and S18 was found to be present among 100 analyzed clones. Levels of ...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Vaarala MH,Porvari KS,Kyllönen AP,Mustonen MV,Lukkarinen O,Vihko PT

    更新日期:1998-09-25 00:00:00

  • Pregnancy outcomes in childhood cancer survivors: probable effects of abdominal irradiation.

    abstract::A postal survey addressed to 2,083 general practitioners of childhood cancer survivors of reproductive age has revealed that females having undergone direct abdominal irradiation (exposed), particularly for Wilms' tumour, have an increased risk of several adverse pregnancy outcomes as compared with female survivors of...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Hawkins MM,Smith RA

    更新日期:1989-03-15 00:00:00

  • Estimation of the effects of smoking and DNA repair capacity on coefficients of a carcinogenesis model for lung cancer.

    abstract::Numerous prospective and retrospective studies have clearly demonstrated a dose-related increased lung cancer risk associated with cigarette smoking, with evidence also for a genetic component to risk. In this study, using the two-stage clonal expansion stochastic model framework, for the first time we investigated th...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Deng L,Kimmel M,Foy M,Spitz M,Wei Q,Gorlova O

    更新日期:2009-05-01 00:00:00

  • A cross-sectional investigation of breast density and insulin-like growth factor I.

    abstract::Our study investigated the association of breast cancer risk as assessed by mammographic density with insulin-like growth factor I (IGF-I) and one of its binding proteins (IGFBP-3) in healthy premenopausal women with different ethnic backgrounds. In a cross-sectional design, we analyzed the baseline mammograms and fas...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Maskarinec G,Williams AE,Kaaks R

    更新日期:2003-12-20 00:00:00

  • Correlation between the serum level of hepatitis C virus RNA and disease activities in acute and chronic hepatitis C.

    abstract::The influence of viremia on hepatic injury in patients infected with hepatitis C virus was examined by analysis of the relationship between alanine aminotransferase activity and the amount of hepatitis C virus RNA in sequential serum samples from I untreated patient with acute hepatitis C and 3 untreated patients with...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Gunji T,Kato N,Mori S,Ootsuyama Y,Hijikata M,Imawari M,Shimotohno K

    更新日期:1992-11-11 00:00:00

  • Specific antigen of murine erythroblasts.

    abstract::A new antigan has been revealed by means of antisera against Rauscher virus in mice with Rauscher virus-induced leukemia. This antigen appears to be different from both Rauscher type-specific antigen and MULV-gs-1 (p-30), as shown by studies of electrophoretic mobility and immunochemical specificity. Except in leukemi...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Ievleva ES,Engelhardt NV,Abelev GI

    更新日期:1976-06-15 00:00:00

  • Cervical carcinoma: detection of herpes simplex virus RNA in cells undergoing neoplastic change.

    abstract::3H-HSV-2 DNA has been hybridized in situ to frozen sectioned tissue from human cervical biopsies. RNA complementary to the virus-specific probe was detected in cells undergoing pre-malignant changes, but not in the cells of the fully developed squamous-cell cancer. ...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: McDougall JK,Galloway DA,Fenoglio CM

    更新日期:1980-01-15 00:00:00

  • Microsatellite analysis and telomerase activity in archived tissue and urine samples of bladder cancer patients.

    abstract::We performed microsatellite analysis and tested telomerase activity in paired tissue and urine of bladder cancer patients from frozen archived samples. DNA obtained from microdissected tumor and urine sediment was analyzed and compared to peripheral lymphocytes for microsatellite alterations (loss of heterozygosity [L...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Linn JF,Lango M,Halachmi S,Schoenberg MP,Sidransky D

    更新日期:1997-12-19 00:00:00

  • Endoscopic screening for synchronous esophageal neoplasia among patients with incident head and neck cancer: Prevalence, risk factors, and outcomes.

    abstract::Esophageal squamous-cell neoplasia (ESCN) is a common second primary neoplasia found in patients with head-and-neck squamous-cell carcinoma (HNSCC). This study sought to identify the risk factors for synchronous ESCN and how they influence survival in HNSCC patient. Eight hundred and fifteen incident HNSCC patients we...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Wang YK,Chuang YS,Wu TS,Lee KW,Wu CW,Wang HC,Kuo CT,Lee CH,Kuo WR,Chen CH,Wu DC,Wu IC

    更新日期:2017-11-15 00:00:00

  • Residential and occupational exposure to 50-Hz magnetic fields and brain tumours in Norway: a population-based study.

    abstract::Our case-control study was conducted to investigate whether residential and occupational exposure to magnetic fields increased the risk for brain tumours in adults. Data from an occupational exposure matrix was also evaluated. The study population in this nested case-control study was made up of subjects aged 16 years...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Klaeboe L,Blaasaas KG,Haldorsen T,Tynes T

    更新日期:2005-05-20 00:00:00

  • The sialosyl Lewis a ganglioside is present in tumorigenic human urothelial cell lines.

    abstract::The sialosyl Lewis a antigen was identified in a ganglioside extract from a malignant human urothelial cell line (Hu 1703He) by fast atom bombardment mass spectrometry. The presence of this antigen in urothelial cell lines with varying tumorigenic properties was further studied using the 19-9 monoclonal antibody (MAb)...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Ugorski M,Påhlsson P,Dus D,Nilsson B,Skouv J,Radzikowski C

    更新日期:1990-06-15 00:00:00