Abstract:
:Very late antigen-4 (VLA-4) composed of alpha 4 and beta 1, a member of the beta 1-integrin subfamily, facilitates cell-to-cell interaction with vascular cell-adhesion molecule-1 (VCAM-1) on endothelial cells (EC). Attachment of blood-borne tumor cells to EC is a crucial step for hematogenous metastasis, and VLA-4-positive tumor cells can attach to EC by binding to VCAM-1. Renal-cell cancer (RCC) reveals proportionally greater percentages of metastases than other carcinomas at initial diagnosis. We investigated whether VLA-4 is expressed on RCC, and how such expression on RCC correlates with the metastatic potential of RCC. Immunohistochemical staining on 66 primary and 4 metastatic RCC showed that 4 out of 4 metastatic and 5 out of 8 primary RCC from patients with lung and/or brain metastases expressed alpha 4 and beta 1 chains. On the other hand, 13 of 58 RCC without metastases expressed alpha 4 chain, alpha 4 and beta 1 expressions were also detected on 5 out of 5 human RCC cell lines, ACHN, KRC/Y, A498, Caki1 and Caki2, by flow-cytometric analysis. Reverse-transcriptase-polymerase-chain-reaction (RT-PCR), followed by Southern-blot hybridization with cDNA probe for a alpha 4 chain, also confirmed mRNA production in 4 out of 5 RCC cell lines. Furthermore, adhesion of alpha 4-positive RCC cell lines to human umbilical-vein endothelial cells (HUVEC) was augmented by treatment of HUVEC with tumor necrosis factor-alpha (TNF-alpha). This adhesion was inhibited by anti-alpha 4 or anti-VCAM-1 antibodies, suggesting that VLA-4-VCAM-1 interaction was involved in the adhesion between RCC cells and HUVEC. Taken together, VLA-4 on RCC cells might play a crucial role in their hematogenous metastasis.
journal_name
Int J Cancerjournal_title
International journal of cancerauthors
Tomita Y,Saito T,Saito K,Oite T,Shimizu F,Sato Sdoi
10.1002/ijc.2910600604subject
Has Abstractpub_date
1995-03-16 00:00:00pages
753-8issue
6eissn
0020-7136issn
1097-0215journal_volume
60pub_type
杂志文章abstract::The impact of the ABC transporters breast cancer resistance protein/mitoxantrone resistance associated transporter (BCRP/MXR), multidrug resistance-associated protein 1 (MRP1) and multidrug resistance gene-1/P-glycoprotein (MDR1/PGP) on the multidrug resistance (MDR) phenotype in chemoresistance and thermoresistance w...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.10131
更新日期:2002-02-20 00:00:00
abstract::Previous studies have revealed a robust association between exposure to asbestos and human lung cancer. Accumulating evidence has highlighted the role of epigenome deregulation in the mechanism of carcinogen-induced malignancies. We examined the impact of asbestos on DNA methylation. Our genome-wide studies (using Ill...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.30897
更新日期:2017-11-15 00:00:00
abstract::To identify CTL-directed antigens in gastrointestinal cancer, we have investigated antigens recognized by the HLA-A2-restricted CTL line established from T cells infiltrating into colon cancer and report herein cleavage and polyadenylation specificity factor (CPSF) as a potent antigen holding peptides capable of induc...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.10377
更新日期:2002-05-20 00:00:00
abstract::The monoclonal antibody (MAb) 4F2 defines an oncofetal antigen in human fibroblastic cells. Two-dimensional electrophoretic analysis reveals that tumor cell lines from mesenchymal tissues co-express two or more heavy-chain molecular variants of the antigen whereas the light subunit (41 kDa) is not affected. Among norm...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910380206
更新日期:1986-08-15 00:00:00
abstract::We present a pilot study that aims to examine the possibility to easily and noninvasively detect and discriminate females with ovarian cancer (OC) from females that have no tumor(s) and from females that have benign genital tract neoplasia, using exhaled breath samples. The study is based on clinical samples and data ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.29166
更新日期:2015-03-15 00:00:00
abstract::Prostate-specific antigen (PSA) has been characterized as a specific prostate marker, although recent studies have suggested the existence of PSA of nonprostatic sources. We have assessed the presence of PSA by an ultrasensitive assay in 276 serum and nonserum samples from women. Our data show the presence of PSA in 9...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0215(19961115)68:4<424::AID-IJC
更新日期:1996-11-15 00:00:00
abstract::We investigated whether the concentration of fibronectin (FN) in the cerebrospinal fluid (CSF) could be used for identifying patients with subclinical blast-cell infiltration in the central nervous system (CNS) and an increased risk of CNS relapse later in the course of their leukemia. Our series comprised 36 children...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910430108
更新日期:1989-01-15 00:00:00
abstract::The presence, concentration and selected molecular characteristics of the human mammary carcinoma glycoprotein molecule set MTGP, a trace and apparently tumor-specific molecule, were examined in fifteen cell cultures established from mammary carcinomas, tissue from seven mammary carcinomas and control cultures. Both c...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910280107
更新日期:1981-07-15 00:00:00
abstract::Tissue recombinants (TRs) composed of mouse urogenital mesenchyme (mUGM) plus an immortalized nontumorigenic human prostatic epithelial cell line (BPH-1) were grown under the kidney capsule of male athymic nude mice under different hormonal conditions. The objectives were to determine temporal plasma concentrations of...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.21614
更新日期:2006-05-01 00:00:00
abstract::A PCR assay using capillary electrophoresis was designed for the detection of c-erbB-2 gene amplification in alcohol-formalin-acetic acid (AFA)-fixed, paraffin-embedded biopsies from 81 consecutive breast tumors. c-erbB-2 expression was analyzed in the same samples using immuno-histochemistry (IHC). In the competitive...
journal_title:International journal of cancer
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1002/(sici)1097-0215(19991008)83:2<157::aid-ijc
更新日期:1999-10-08 00:00:00
abstract::Breast cancer can be classified into molecular sub-types that have distinct survival patterns. We evaluated the prognostic significance of breast cancer sub-types in a cohort of women taking part in the NEAT and BR9601 clinical trials comparing cyclophosphamide, methotrexate and fluorouracil (CMF) with ECMF (epirubici...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.28150
更新日期:2013-09-15 00:00:00
abstract::Conventionally bred rats possess in their sera an NA which has a cytotoxic effect on a tumor cell line (KMT-17) derived from a fibrosarcoma induced by 3-methylcholanthrene and which can be absorbed by normal rat tissues. We have succeeded in purifying a glycosphingolipid as an antigen reactive to NA. GSLs isolated fro...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910360613
更新日期:1985-12-15 00:00:00
abstract::Evidence of the association between chronic inflammation and the risk of colorectal cancer (CRC) and other obesity-related cancers (OBRC) remains inconsistent, possibly due to a paucity of studies examining repeated measures of inflammation. In the Health ABC prospective study of 2,490 adults aged 70-79 years at basel...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.29868
更新日期:2016-03-01 00:00:00
abstract::Increasing use of heavy ions for cancer therapy and concerns from exposure to heavy charged particles in space necessitate the study of the basic biological mechanisms associated with exposure to heavy ions. As the most critical damage induced by ionizing radiation is DNA double strand break (DSB), this review focuses...
journal_title:International journal of cancer
pub_type: 杂志文章,评审
doi:10.1002/ijc.26445
更新日期:2012-03-01 00:00:00
abstract::Interval breast cancer (IC) has a more aggressive phenotype and higher mortality than screen-detected cancer (SDC). In this case-case study, we investigated whether the size of longitudinal fluctuations in mammographic percent density (PD fluctuation) was associated with the ratio of IC versus SDC among screened women...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.30427
更新日期:2017-01-01 00:00:00
abstract::Signal transducer and activator of transcription 5b (STAT5b) is likely the relevant STAT5 isoform with respect to the process of malignant progression in gliomas. STAT5b is a latent cytoplasmic protein involved in cell signaling through the modulation of growth factors, apoptosis, and angiogenesis. Previous in vitro s...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.29264
更新日期:2015-05-01 00:00:00
abstract::The steady-state mRNA level of nucleophosmin/B23 in HeLa cells increased after UV irradiation. Nucleophosmin/B23 antisense transfection potentiated ultraviolet (UV)-induced cell killing. A block in G(2)/M phase, larger peak of apoptotic cells and higher caspase-3 in vitro activity were noted in nucleophosmin/B23 antis...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.1606
更新日期:2002-01-20 00:00:00
abstract::We evaluated promoter hypermethylation of a panel of tumor suppressor genes as a means to detect epigenetic alterations in oral squamous cell carcinomas (OSCC) of Indian-origin and compare with North-American head and neck squamous cell carcinomas (HNSCC). Quantitative-methylation-specific PCR was used to investigate ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.25377
更新日期:2010-11-15 00:00:00
abstract::In 1927 Otto Warburg established that tumours derive energy primarily from the conversion of glucose to lactic acid and only partially through cellular respiration involving oxygen. In the 1950s he proposed that all causes of cancer reflected different mechanisms of disabling cellular respiration in favour of fermenta...
journal_title:International journal of cancer
pub_type: 杂志文章,评审
doi:10.1002/ijc.29012
更新日期:2015-10-01 00:00:00
abstract::It is well known that tumors arising from tissues such as kidney, pancreas, liver and stomach are particularly refractory to treatment. Searching for new anticancer drugs using cells in culture has yielded some effective therapies, but these refractory tumors remain intractable. Studies that compare cells grown in sus...
journal_title:International journal of cancer
pub_type: 杂志文章,评审
doi:10.1002/ijc.20671
更新日期:2005-03-01 00:00:00
abstract::Homozygous deletion and loss of heterozygosity (LOH) at chromosome 3p21 have been observed in several types of human cancer including lung cancer and breast cancer. In previous work, we cloned and identified the human RAS association domain family 1A gene (RASSF1A) from the lung tumor suppressor locus 3p21.3. The CpG ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.1466
更新日期:2001-10-15 00:00:00
abstract::Withaferin A (WFA) is purified from the plant Withania somnifera and inhibits the vimentin cytoskeleton. Vimentin overexpression in cancer correlates with metastatic disease, induction of epithelial to mesenchymal transition and reduced patient survival. As vimentin functions in cell motility, we wanted to test the hy...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.25938
更新日期:2011-12-01 00:00:00
abstract::Human epidermoid carcinoma A431 cells attach more rapidly to collagen type-I and -IV substrates than to surfaces coated with laminin or fibronectin. The diminished intercellular interaction and rounding up manifested when these cells are exposed to epidermal growth factor (EGF) in tissue culture plastic or collagen fi...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910370313
更新日期:1986-03-15 00:00:00
abstract::Evidence indicates that acquired resistance of cancers to chemotherapeutic agents can occur via epigenetic mechanisms. Down-regulation of expression of argininosuccinate synthetase (ASS1), the rate-limiting enzyme in the biosynthesis of arginine, has been associated with the development of platinum resistance in ovari...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.24546
更新日期:2009-09-15 00:00:00
abstract::To identify genes associated with radioresistant oral squamous cell carcinoma (OSCC), we compared gene expression signatures between OSCC cell lines exhibiting radioresistance and cells with radiosensitivity after X-ray irradiation in a dose-dependent manner using Affymetrix GeneChip analysis with Human Genome-U133 pl...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.22561
更新日期:2007-05-15 00:00:00
abstract::Leukemia stem cells (LSCs) might originate from malignant transformation of normal hematopoietic stem cells (HSCs), or alternatively, of progenitors in which the acquired mutations have re-installed a dysregulated self-renewal program. LSCs are on top of a hierarchy and generate leukemia cells with more differentiated...
journal_title:International journal of cancer
pub_type: 杂志文章,评审
doi:10.1002/ijc.26318
更新日期:2011-11-15 00:00:00
abstract::Human papillomavirus (HPV) vaccination of a birth cohort of girls in the 9-13 age range is recommended as a priority, but decreases in HPV vaccine cost may make catch-up of a few additional cohorts more attractive not only in high-income countries. We assessed the reduction in HPV16 and 18 infections that could be ach...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.28197
更新日期:2013-10-15 00:00:00
abstract::There are approximately 100 genes which when mutated are known to predispose to one or more forms of cancer. Currently, genetic testing is offered for many of these, either as single genes or as multi-gene panels. Features of hereditary cancer include a positive family history of cancer, early age of onset and the app...
journal_title:International journal of cancer
pub_type: 杂志文章,评审
doi:10.1002/ijc.28988
更新日期:2014-10-15 00:00:00
abstract::During 2000, marine antitumor pharmacology research aimed at the discovery of novel antitumor agents was published in 85 peer-reviewed articles. The purpose of this article is to present a structured review of the antitumor and cytotoxic properties of 143 marine natural products, many of them novel compounds that belo...
journal_title:International journal of cancer
pub_type: 杂志文章,评审
doi:10.1002/ijc.11080
更新日期:2003-06-20 00:00:00
abstract::Monoclonal antibodies (MAbs) reactive with the ganglioside fucosyl GM1 (Fuc-GM1), an antigen associated with small-cell carcinoma of the lung (SCLC), were tested for their ability to mediate tumor-cell killing in vitro in conjunction with humoral and cellular effectors and to inhibit tumor engraftment in nude mice in ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910490619
更新日期:1991-12-02 00:00:00