Abstract:
OBJECTIVE:Hyaluronan (HA) plays a role in keratinocyte proliferation and differentiation. In addition, HA has been shown to have different biological activities depending on its molecular weight. It has been reported that HA-mediated CD44 activation regulates keratinocyte differentiation. Therefore, the aim of this study was to investigate the influence of HA tetrasaccharides (HA4) on the regulation of keratinocyte differentiation, CD44 gene expression and CD44-phosphorylated protein in human keratinocytes, and compare HA4 with high molecular weight HA. METHODS:Normal human epidermal keratinocytes (NHEKs) were treated at doses of 1 μg mL(-1) HA or HA oligosaccharides (HA4). After treatment, cell viability was checked using an MTT (3-(4,5-di-methylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Each differentiation marker and CD44 mRNA expression was detected by real-time PCR. Each differentiation marker and CD44-phosphorylated protein was assessed by Western blotting. RESULTS:Hyaluronan and HA4 showed no cytotoxicity up to a dose of 1 μg mL(-1) . On day 3 after HA4 treatment, each differentiation marker mRNA and K10 protein level was higher than that of the control. On day 9, late differentiation marker mRNA and protein levels were increased with HA and HA4 treatment. In addition, HA4 treatment increased the expression of CD44 mRNA, CD44-phosphorylated protein and intracellular calcium concentrations. HA4 enhanced keratinocyte differentiation and increased CD44-phosphorylated protein levels. CONCLUSION:HA4 may induce epidermal differentiation through phosphorylation of CD44.
journal_name
Int J Cosmet Scijournal_title
International journal of cosmetic scienceauthors
Kage M,Tokudome Y,Matsunaga Y,Hariya T,Hashimoto Fdoi
10.1111/ics.12105subject
Has Abstractpub_date
2014-02-01 00:00:00pages
109-15issue
1eissn
0142-5463issn
1468-2494journal_volume
36pub_type
杂志文章abstract::Various models of reconstructed epidermis already provide useful tools for safety and efficacy assessment of cosmetic products. However, the majority of these in vitro models are composed of keratinocytes only. Recently, the introduction of melanocytes into epidermal reconstructs has considerably enlarged their field ...
journal_title:International journal of cosmetic science
pub_type: 杂志文章
doi:10.1046/j.1467-2494.1999.183571.x
更新日期:1999-02-01 00:00:00
abstract:OBJECTIVE:Although the olfactory receptor OR2AT4 was described as involved in epidermal renewal, there is no data about a cosmetic active ingredient activating this receptor. The aim of this research work was thus to identify a natural molecule binding to this receptor in order to stimulate keratinocyte migration. MET...
journal_title:International journal of cosmetic science
pub_type: 杂志文章
doi:10.1111/ics.12526
更新日期:2019-04-01 00:00:00
abstract:BACKGROUND:Cosmetics are products used over long periods by the public, and their safety is very important. Several types of human tests are used widely for the evaluation of cosmetics including single patch tests, in-use tests, human repeated insult patch test (HRIPT). However, there is no clear and well-defined publi...
journal_title:International journal of cosmetic science
pub_type: 杂志文章
doi:10.1111/ics.12095
更新日期:2014-02-01 00:00:00
abstract::The aim of this study was to determine how worthwhile it would be to combine a newly developed topical slimming product with customized dietary habits not based on calorie restriction, so as to improve the cellulite appearance of the skin. At the beginning of the study, a nutritionist recorded the dietary habits of ea...
journal_title:International journal of cosmetic science
pub_type: 杂志文章,随机对照试验
doi:10.1111/j.1468-2494.2010.00630.x
更新日期:2011-08-01 00:00:00
abstract:INTRODUCTION:We report on the preparation and efficacy of 10-hydroxystearic acid (HSA) that improves facial age spots and conspicuous pores. METHODS:The hydration of oleic acid into HSA was catalyzed by the oleate hydratase from Escherichia coli. Following treatment with HSA, collagen type I and type III was assessed ...
journal_title:International journal of cosmetic science
pub_type: 杂志文章
doi:10.1111/ics.12529
更新日期:2019-06-01 00:00:00
abstract:OBJECTIVE:The aim of the study was to determine the optimal conditions, that is the content of hemp oil and time of homogenization to obtain stable dispersion systems. METHODS:For this purpose, six emulsions were prepared, their stability was examined empirically and the most correctly formulated emulsion composition ...
journal_title:International journal of cosmetic science
pub_type: 杂志文章
doi:10.1111/ics.12211
更新日期:2015-08-01 00:00:00
abstract::Skin acts a major target as well as a principle barrier for topical/transdermal drug delivery. Despite the many advantages of this system, the major obstacle is the low diffusion rate of drugs across the stratum corneum. Several methods have been assessed to increase the permeation rate of drugs temporarily. One simpl...
journal_title:International journal of cosmetic science
pub_type: 杂志文章
doi:10.1111/j.1467-2494.2005.00264.x
更新日期:2005-08-01 00:00:00
abstract::Synopsis The mechanism of colour change during the pigment dispersion process and during powder application to the skin surface was studied by examining the colour extension properties of Yellow. Red, and Black iron oxides in white powder bases. A new measure for the evaluation of the degree of colour extension was de...
journal_title:International journal of cosmetic science
pub_type: 杂志文章
doi:10.1111/j.1467-2494.1985.tb00391.x
更新日期:1985-02-01 00:00:00
abstract::Kajal and surma are eye cosmetics extensively used in Indian subcontinent. Kajal is prepared by burning of vegetable oil and butter oil while surma by grinding of the stones. High performance liquid chromatography and gas chromatography-mass spectrometry instruments were used for quantification and confirmation of 16 ...
journal_title:International journal of cosmetic science
pub_type: 杂志文章
doi:10.1111/j.1468-2494.2009.00497.x
更新日期:2009-06-01 00:00:00
abstract::This study was a pilot project, set up to assess ageing skin using a multi-disciplinary approach. The main aim of this study was to evaluate whether the use of more radical ('medical') treatments in the management of skin ageing would bring superior results and ultimately make people look younger, than the use of cosm...
journal_title:International journal of cosmetic science
pub_type: 杂志文章
doi:10.1111/j.1468-2494.2012.00741.x
更新日期:2012-10-01 00:00:00
abstract::Oleuropein, a phenolic compound derived from olive leaves and oil, is known to possess several biological properties, many of which may be attributed to its antioxidant and free radical-scavenging activities. Nevertheless, up to now, the cosmetic activity of this molecule has not been extensively investigated. The aim...
journal_title:International journal of cosmetic science
pub_type: 临床试验,杂志文章
doi:10.1111/j.1468-2494.2008.00424.x
更新日期:2008-04-01 00:00:00
abstract::Water homeostasis of the epidermis is important for the appearance and physical properties of the skin, as well as for water balance in the body. It depends on several factors, e.g. barrier quality, uptake of water into the epidermis, concentration of water-retaining humectants, and external humidity. Aquaporins (AQPs...
journal_title:International journal of cosmetic science
pub_type: 杂志文章
doi:10.1111/j.1468-2494.2007.00402.x
更新日期:2007-12-01 00:00:00
abstract:OBJECTIVE:As skin ages, impaired extracellular matrix (ECM) protein synthesis and increased action of degradative enzymes manifest as atrophy, wrinkling and laxity. There is mounting evidence for the functional role of exogenous peptides across many areas, including in offsetting the effects of cutaneous ageing. Here, ...
journal_title:International journal of cosmetic science
pub_type: 杂志文章
doi:10.1111/ics.12635
更新日期:2020-08-01 00:00:00
abstract::Reduction and prevention of health care-associated infections is a worldwide priority with emphasis on increasing hand hygiene compliance. Repetitive exposure to hand hygiene products and procedures is a significant factor in the development of occupational irritant hand dermatitis. Compliance has been difficult to ac...
journal_title:International journal of cosmetic science
pub_type: 杂志文章,评审
doi:10.1111/j.1468-2494.2012.00733.x
更新日期:2012-10-01 00:00:00
abstract::Stratum corneum (SC) bilayer lipids, specifically fatty acids, ceramides and cholesterol, contribute to the permeability barrier function of the skin. Normal skin cleansing is associated with damage to the SC lipids because cleanser surfactants, in addition to providing the desired effect of solubilizing and facilitat...
journal_title:International journal of cosmetic science
pub_type: 杂志文章,评审
doi:10.1111/ics.12042
更新日期:2013-08-01 00:00:00
abstract::Synopsis The purpose of this work was to compare the evolution of three multiple emulsions (ME) by different controls. The series of water-in-oil-in-water (W/O/W) multiple emulsions were elaborated and controlled by both the usual analyses which permitted the classification of the multiple emulsions with respect to th...
journal_title:International journal of cosmetic science
pub_type: 杂志文章
doi:10.1111/j.1467-2494.1993.tb00069.x
更新日期:1993-04-01 00:00:00
abstract::On a daily basis, the skin is exposed to many environmental stressors and insults. Over a 24-h natural cycle, during the day, the skin is focused on protection; while at night, the skin is focused on repairing damage that occurred during daytime and getting ready for the next morning. Circadian rhythm provides the pre...
journal_title:International journal of cosmetic science
pub_type: 杂志文章
doi:10.1111/ics.12572
更新日期:2019-12-01 00:00:00
abstract::It is well known that the shelf-life of products containing dihydroxyacetone (DHA) is quite unsatisfactory, and that in this area the adjustment to an acidic pH is the way usually recommended to improve DHA stability. The adoption of this rule, however, is a challenge for the cosmetic chemist not only because of the r...
journal_title:International journal of cosmetic science
pub_type: 杂志文章
doi:10.1111/j.1467-2494.2007.00381.x
更新日期:2007-06-01 00:00:00
abstract::Synopsis The principles on which stability tests are based are reviewed. The need to differentiate between product stability and product-container compatibility is emphasized. Recognition of this distinction is fundamental to the efficient organisation and execution of stability tests. Attention is also drawn to the d...
journal_title:International journal of cosmetic science
pub_type: 杂志文章
doi:10.1111/j.1467-2494.1985.tb00423.x
更新日期:1985-12-01 00:00:00
abstract:OBJECTIVE:Zinc pyrithione (ZnPT) is widely used as an anti-fungal active in commercial anti-dandruff (AD) shampoos. The AD efficacy of ZnPT is highly dependent on the deposition of ZnPT particles onto the scalp during the process of shampoo application and rinse-off. Since ZnPT materials with different particle sizes a...
journal_title:International journal of cosmetic science
pub_type: 杂志文章
doi:10.1111/ics.12493
更新日期:2018-10-01 00:00:00
abstract::In this work, a comparative study between two methods to acquire relevant information about a cosmetic formulation has been carried out. A Design of Experiments (DOE) has been applied in two stages to a capillary cosmetic cream: first, a Plackett-Burman (PB) design has been used to reduce the number of variables to be...
journal_title:International journal of cosmetic science
pub_type: 杂志文章
doi:10.1111/j.1468-2494.2009.00565.x
更新日期:2010-10-01 00:00:00
abstract:OBJECTIVE:Recently, our group reported that extracts prepared from the Australian native plant Terminalia ferdinandiana Exell. are potent inhibitors of the growth malodorous bacteria with similar efficacy to triclosan and through these results, we highlighted a potential biological alternative to the current chemical a...
journal_title:International journal of cosmetic science
pub_type: 杂志文章
doi:10.1111/ics.12567
更新日期:2019-10-01 00:00:00
abstract:BACKGROUND:In order to comply with the European legislation concerning the risk assessment of skin sensitizers, considerable progress has been made in developing alternative methods, such as human cell line activation test (h-CLAT). H-CLAT is based on cytometric measurement of fluorescence emitted by anti-CD54 and anti...
journal_title:International journal of cosmetic science
pub_type: 杂志文章
doi:10.1111/ics.12530
更新日期:2019-06-01 00:00:00
abstract:OBJECTIVE:Cutaneous disorders can impact on the psycho-social well-being of children and adults alike, which has been reviewed elsewhere (Body Image 27, 2018 and 98). The present review sets out to evaluate progressions in skin masking and camouflaging techniques together with evaluating the efficacy of these. There do...
journal_title:International journal of cosmetic science
pub_type: 杂志文章,评审
doi:10.1111/ics.12575
更新日期:2019-12-01 00:00:00
abstract::Cocamidopropyl betaine (CAPB) has been the most important secondary surfactant for personal-cleansing products for a long time. Its excellent toxicological profile is an important reason for its increasing use in oral-care products. Recently it has gained interest for further applications such as household cleaners, d...
journal_title:International journal of cosmetic science
pub_type: 杂志文章
doi:10.1046/j.1467-2494.1997.171719.x
更新日期:1997-10-01 00:00:00
abstract::Synopsis Oil/water (o/w) microemulsions containing propranolol were studied. Isopropylmyristate was used as the oil, Tween 60 as a surfactant, butanol as a co-surfactant and a buffer of pH 6.5 for the continuous phase. The lipophilicity of propranolol was enhanced by formation of lipophilic ion-pairs to obtain a dispe...
journal_title:International journal of cosmetic science
pub_type: 杂志文章
doi:10.1111/j.1467-2494.1988.tb00026.x
更新日期:1988-12-01 00:00:00
abstract:BACKGROUND:The effect of photodamage on facial stratum corneum (SC) is still poorly understood. OBJECTIVE:To describe the SC proteome from tape strippings of Caucasian SC from photoexposed cheek and photoprotected post-auricular (PA) site, a global analysis of photodamage on the skin will be developed leading to a bet...
journal_title:International journal of cosmetic science
pub_type: 杂志文章
doi:10.1111/ics.12426
更新日期:2017-12-01 00:00:00
abstract::Hexamidine (HEX) has been used as a preservative in topical preparations since the 1950s. A number of studies also indicate that the molecule plays a beneficial role in skin homoeostasis. In this review, we describe the physicochemical properties of hexamidine diisethionate (HEX D) and the corresponding hydrochloride ...
journal_title:International journal of cosmetic science
pub_type: 杂志文章,评审
doi:10.1111/ics.12392
更新日期:2017-08-01 00:00:00
abstract::It is well established that the evaporation of sweat from the human body surface is the main mechanism by which heat balance is maintained following a rise in body core temperature. Since the introduction of the first brand name antiperspirant in the United States during the early 1900s, antiperspirant products design...
journal_title:International journal of cosmetic science
pub_type: 杂志文章
doi:10.1046/j.1467-2494.2003.00184.x
更新日期:2003-08-01 00:00:00
abstract::Synopsis The ability of baseline transepidermal water loss (TEWL) measurements to predict the response of healthy subjects to a standard soap chamber irritation insult was evaluated. Chambers containing surfactant solutions were applied to the forearms of normal volunteers for five consecutive days. TEWL was measured ...
journal_title:International journal of cosmetic science
pub_type: 杂志文章
doi:10.1111/j.1467-2494.1986.tb00452.x
更新日期:1986-10-01 00:00:00