Abstract:
:In previous studies we showed that human sarcoma and melanoma cell lines synthesize and secrete into culture medium a glycoprotein, migrating in urea sodium dodecyl sulfate-polyacrylamide gel electrophoresis at Mr 140,000. It is not detected in cultures of the corresponding normal cells. Conditioned medium of the melanoma cell line HMB-2, producing among the cell lines tested the largest amounts of this glycoprotein, has now been used as a source for purification of the protein. NH2-terminal amino-acid sequence determination of the purified glycoprotein showed that it is identical to human alpha 2-macroglobulin (alpha 2M). Rabbit antibodies raised against the glycoprotein specifically reacted in immunoblotting and immunodiffusion tests with alpha 2M present in human plasma. Likewise, these antibodies immunoprecipitated from the conditioned media of 35S-methionine-labelled melanoma and osteosarcoma cell lines the protein which had a molecular weight corresponding to alpha 2M. alpha 2M was also synthesized and secreted by 2 strains of fetal lung fibroblasts but not by fetal skin fibroblasts or adult skin fibroblasts autologous to the osteosarcoma cell line.
journal_name
Int J Cancerjournal_title
International journal of cancerauthors
Bízik J,Vaheri A,Saksela O,Kalkkinen N,Meri S,Grófóva Mdoi
10.1002/ijc.2910370114subject
Has Abstractpub_date
1986-01-15 00:00:00pages
81-8issue
1eissn
0020-7136issn
1097-0215journal_volume
37pub_type
杂志文章abstract::Rhabdomyosarcoma (RMS) is the most common soft tissue sarcoma in children. To improve existing therapies and broaden the spectrum of cytotoxic agents that can be used in RMS treatment, we performed a phage-display-based screening for peptides that bind specifically to RMS cells. Two peptides binding to RMS and to othe...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.24170
更新日期:2009-05-01 00:00:00
abstract::The INK4A gene, localized to human chromosome 9p21, encodes p16INK4A, a tumor suppressor that functions at least in part through the inhibition of CDK4, a cyclin-dependent kinase encoded by a gene at 12q13. To examine INK4A gene alterations in uncultured samples of osteosarcoma and the relationship between INK4A and C...
journal_title:International journal of cancer
pub_type: 临床试验,杂志文章
doi:10.1002/(sici)1097-0215(19990118)80:2<199::aid-ijc
更新日期:1999-01-18 00:00:00
abstract::NY-ESO-1 antigen is a prototype of a class of cancer/testis antigens. We carried out a clinical trial using NY-ESO-1 whole protein as a cancer vaccine for 13 advanced cancer patients. We have recently reported that vaccine elicited humoral and cellular immune responses in 9 cancer patients including 4 esophageal cance...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.23810
更新日期:2008-11-15 00:00:00
abstract::Although reproductive factors have been shown to be related to the composition of bile and functioning of the biliary system, their relationship with biliary tract cancer has not been studied in detail. Between 1984 and 1987 we conducted a case-control study of 75 women with cancer of the biliary tract and 252 control...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910570203
更新日期:1994-04-15 00:00:00
abstract::People living with lymphohematopoietic neoplasms (LHNs) are known to have increased risks of second cancer; however, the incidence of second cancers after LHNs has not been studied extensively in Australia. The Australian Cancer Database was used to analyze site-specific risk of second primary cancer after LHNs in 127...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.25706
更新日期:2011-08-15 00:00:00
abstract::The stability of expression of a membrane antigen on human FME melanoma cells was investigated by means of flow cytometric cell sorting and analysis. The melanoma-associated 250 kd antigen was strongly expressed on all cells, as recognized by binding of the monoclonal antibody 9.2.27. By flow cytometric cell sorting, ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910340413
更新日期:1984-10-15 00:00:00
abstract::We have reported the establishment of a mitomycin-C (MMC)-resistant non-small-cell lung-cancer cell line, PC-9/MC4. As determined by an MTT assay, this resistant cell line was found to be 4 times more sensitive to adriamycin (ADM) than was the parental PC-9. There were no significant differences in sensitivity to etop...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910590211
更新日期:1994-10-15 00:00:00
abstract::By using a functional complementation approach, suppression of tumorigenicity was observed after transfer of intact or truncated copies of chromosome 3 into a nasopharyngeal carcinoma (NPC) HONE1 cell line. The extra exogenous chromosome 3 in the microcell hybrids (MCHs) significantly extended the lag period of tumor ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.23528
更新日期:2008-07-15 00:00:00
abstract::The object of our multicenter retrospective study was to compare the new histologic World Health Organization (WHO) classification and the classical histologic Bernatz classification in terms of interobserver agreement and prognostic importance. The influence of coexisting diseases was also analyzed using the Charlson...
journal_title:International journal of cancer
pub_type: 杂志文章,多中心研究
doi:10.1002/ijc.10255
更新日期:2002-04-20 00:00:00
abstract::Specific types of human papillomaviruses (HPV) are associated with most cases of pre-invasive and invasive neoplasia of the uterine cervix. HLA phenotype influences susceptibility and resistance to viral infections and may therefore influence the course of HPV-associated tumors. Some data suggest that specific HLA cla...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0215(19960621)69:3<159::AID-IJC
更新日期:1996-06-21 00:00:00
abstract::We have established human oral-squamous-cancer cell lines, BHY and HN, derived from non-metastatic cancer and metastatic cancer respectively. We examined the expression of matrix-degrading enzymes and their inhibitors in these cell lines. Both cell lines expressed pro-matrix metalloproteinase (MMP)1, proMMP2, proMMP9,...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19970106)70:1<120::aid-ijc
更新日期:1997-01-06 00:00:00
abstract::Using a recently developed autochthonous mouse model of squamous cell carcinoma (SCC), a combination therapy targeting polyamine metabolism was evaluated. The therapy combined 2-difluoromethylornithine (DFMO), an inhibitor of ornithine decarboxylase (ODC), and MQT 1426, a polyamine transport inhibitor. In 1 trial last...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.21621
更新日期:2006-05-01 00:00:00
abstract::We recently reported that standardized quantitative immunohistochemical (IHC) assays allowed prediction of an adverse outcome among 572 node negative (N-) patients with breast carcinoma (BrCa). To further validate our prior findings, we repeated the IHC stains including a second series of BrCa diagnosed at Yale Univer...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.27371
更新日期:2012-08-01 00:00:00
abstract::Estimates of the worldwide incidence and mortality from 36 cancers and for all cancers combined for the year 2018 are now available in the GLOBOCAN 2018 database, compiled and disseminated by the International Agency for Research on Cancer (IARC). This paper reviews the sources and methods used in compiling the cancer...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.31937
更新日期:2019-04-15 00:00:00
abstract::Human conventional renal cell carcinoma (CCC) remains resistant to current therapies. Focal Adhesion Kinase (FAK) is upregulated in many epithelial tumors and clearly implicated in nearly all facets of cancer. However, only few reports have assessed whether FAK may be associated with renal tumorigenesis. In this study...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.29522
更新日期:2015-10-01 00:00:00
abstract::Akt is a promising therapeutic target for cancer treatment. In our study, we have identified that 7-deoxynarciclasine (7-DONCS) is a potential inhibitor of Akt, which results in the repression of multiple oncogenic processes in hepatocellular carcinoma (HCC). We have found that 7-DONCS suppresses the growth of HCC by ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.32395
更新日期:2019-12-15 00:00:00
abstract::The calcium-sensing receptor (CaSR) is suggested to mediate the antiproliferative effects of calcium in colon. However, in colorectal cancer (CRC) the expression of the CaSR is silenced and the underlying mechanisms leading to its loss are poorly understood. We investigated whether loss of the CaSR expression in color...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.28856
更新日期:2014-11-01 00:00:00
abstract::Mouse and human lymphoid cells were cultivated in the presence of T-cell growth factor (TCGF) and evaluated for their in vivo anti-tumor effect in mice. Cultured spleen cells of normal BALB/c mice or of mice bearing the M109 tumor had a high level of cytotoxic activity in vitro against a variety of tumor target cells ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910280208
更新日期:1981-08-15 00:00:00
abstract::Exfoliated nasopharyngeal (NP) cells from 62 normal Cantonese Chinese having IgA/VCA antibodies for more than a year and from 39 similar persons without IgA/VCA antibodies, were tested for the presence of EBV/DNA sequences by spot followed by blot hybridization tests, using the cloned internal repeat of B95-8 viral DN...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910320504
更新日期:1983-11-15 00:00:00
abstract::We have addressed the role of bacterial co-infection in viral oncogenesis using as model Epstein-Barr virus (EBV), a human herpesvirus that causes lymphoid malignancies and epithelial cancers. Infection of EBV carrying epithelial cells with the common oral pathogenic Gram-negative bacterium Aggregatibacter actinomycet...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.31652
更新日期:2019-01-01 00:00:00
abstract::The expression of oestrogen (ER), progesterone (PR) and androgen (AR) receptors in female breast cancer was investigated by immunohistochemistry on snap-frozen tissue specimens of a series of 100 breast cancers. For detection of the AR we used a recently developed mouse monoclonal antibody specific for the N-terminal ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910520415
更新日期:1992-10-21 00:00:00
abstract::The CpG island methylator phenotype (CIMP) with widespread promoter CpG island methylation is a phenotype in colorectal cancer, associated with microsatellite instability (MSI) and BRAF mutation. Genome-wide hypomethylation may also play an important role in genomic instability. However, the relation between global DN...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.23470
更新日期:2008-06-15 00:00:00
abstract::Human papillomavirus (HPV) genomes usually persist as episomal molecules in HPV associated preneoplastic lesions whereas they are frequently integrated into the host cell genome in HPV-related cancers cells. This suggests that malignant conversion of HPV-infected epithelia is linked to recombination of cellular and vi...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:
更新日期:2001-04-01 00:00:00
abstract::MET is a master gene controlling a genetic program driving proliferation, apoptosis protection and invasion. The ROR1 pseudokinase acts as a MET substrate. However, its contribution to MET signaling and MET-dependent biological outcomes remains to be elucidated. By structure-function analysis of ROR1 mutants, we show ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.28879
更新日期:2014-11-15 00:00:00
abstract::I-compounds are bulky indigenous DNA adducts that can be detected by (32)P-postlabeling. A subgroup, termed type II I-compounds, represents DNA lesions induced by oxidative stress. Several major type II I-compounds have been identified as dinucleotides containing 3'-terminal 8,5'-cyclo-2'-deoxyadenosine (cA). Levels o...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.20375
更新日期:2004-12-10 00:00:00
abstract::Paclitaxel is widely used to treat cancer patients through the blocking of mitosis and result in formation of polyploidy giant cancer cells (PGCCs), which are generally believed to be nondividing cells or in mitotic catastrophe. Here, we showed that PGCCs following the treatment of paclitaxel of MCF-7 breast cancer ce...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.28319
更新日期:2014-02-01 00:00:00
abstract::Colorectal (CRC) and gastric cancers (GC), the most common gastrointestinal malignancies, have been known to develop occasionally in a same patient. Previous studies have focused on the etiology of patients with multiple primary gastric and colorectal cancer (MPGCC); however, the carcinogenic process of MPGCC remains ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(20000601)86:5<678::aid-ijc
更新日期:2000-06-01 00:00:00
abstract::Data from an international case-control study of breast cancer that was conducted in the 1960s were used to examine whether multiple births affect the risk of breast cancer in the mother. Among 2821 parous breast-cancer patients, 88 had had one or more multiple births, whereas among 8882 controls 247 had had one or mo...
journal_title:International journal of cancer
pub_type: 杂志文章,多中心研究
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更新日期:1993-04-22 00:00:00
abstract::In light of recent, strong inverse findings between lifetime red wine consumption and prostate cancer among younger men, we revisited our previous cohort analysis to more thoroughly investigate red wine consumption and prostate cancer in the Health Professionals Follow-up Study (HPFS). In 1986, HPFS participants repor...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.22498
更新日期:2007-04-01 00:00:00
abstract::While the association between fruit consumption and bladder cancer risk has been extensively reported, studies have had inadequate statistical power to investigate associations between types of fruit and bladder cancer risk satisfactorily. Fruit consumption in relation to bladder cancer risk was investigated by poolin...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.33008
更新日期:2020-10-15 00:00:00