Validation of an immunohistochemical signature predictive of 8-year outcome for patients with breast carcinoma.

Abstract:

:We recently reported that standardized quantitative immunohistochemical (IHC) assays allowed prediction of an adverse outcome among 572 node negative (N-) patients with breast carcinoma (BrCa). To further validate our prior findings, we repeated the IHC stains including a second series of BrCa diagnosed at Yale University. Tissue microarrays (TMAs) of two cohorts of patients with BrCa (418 Marseille University and 303 Yale University) were respectively investigated for IHC expression of 15 markers (HIF-1α, PI3K, pAKT, pmTOR, moesin, P21, 4(E) BP-1, P27, Ker5-6, pMAPKAPK-2, SHARP2, claudin-1, ALDH, AF6 and CD24). Quantitative measurements of immunoprecipitates densitometry assessed with an image analyzer were correlated with 8-year patients' outcome and compared in the two cohorts. The best predictive signature consisted of a combination of five markers that included HIF-1α, PI3K, claudin-1, AF6 and pAKT in N- BrCa. This combination permitted an accurate prediction of outcome in 92.34% (386/418) of N- patients in the first set (Marseille) and 89.8% (158/176) in the second set (Yale). The close results in both cohorts confirmed the validity of this original IHC signature predictive of prognosis in node negative BrCa. This validation suggests that in clinical practice, it would be possible with standardized kits (i) to identify patients with poor prognosis at diagnosis time, particularly in the N- BrCa subset, who would require more aggressive adjuvant therapy and (ii) to avoid useless expensive therapies and their side effects in N- patients with favorable prognosis.

journal_name

Int J Cancer

authors

Charpin C,Tavassoli F,Secq V,Giusiano S,Villeret J,Garcia S,Birnbaum D,Bonnier P,Lavaut MN,Boubli L,Carcopino X,Iovanna J

doi

10.1002/ijc.27371

subject

Has Abstract

pub_date

2012-08-01 00:00:00

pages

E236-43

issue

3

eissn

0020-7136

issn

1097-0215

journal_volume

131

pub_type

杂志文章
  • Study of Epstein-Barr virus-determined nuclear antigen (EBNA) by chromatography on fixed cell nuclei.

    abstract::Low ionic strength (50 to 100 mM NaCl) and pH 6.0 were found to be optimal conditions for in vitro conversion of Epstein-Barr virus (EBV)-determined nuclear antigen (EBNA)-negative nuclei to EBNA-positive nuclei by addition of the complement-fixing (CF) antigen extracted from Raji cells. In vitro conversion of nuclei ...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/ijc.2910220505

    authors: Hirsch I,Suchánková A,Závadová H,Vonka V

    更新日期:1978-11-15 00:00:00

  • Identification of a subset of pericytes that respond to combination therapy targeting PDGF and VEGF signaling.

    abstract::The aim of our study was to further explore the use of anti-angiogenic therapy targeting the vascular endothelial growth factor receptor (VEGFR) on endothelial cells while simultaneously targeting platelet-derived growth factor receptors (PDGFRs) on adjacent pericytes. B16 mouse melanoma tumors exogenously expressing ...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/ijc.22999

    authors: Hasumi Y,Kłosowska-Wardega A,Furuhashi M,Ostman A,Heldin CH,Hellberg C

    更新日期:2007-12-15 00:00:00

  • Analysis of proviral integration in human mammary epithelial cell lines immortalized by retroviral infection with a temperature-sensitive SV40 T-antigen construct.

    abstract::A panel of eight conditionally immortal lines derived by infection of human breast epithelial cells with an amphotropic retrovirus transducing a ts mutant of SV40 large T-antigen was analyzed with respect to individual retroviral integration patterns. Each line contained multiple integration sites which were clonal an...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/ijc.2910570616

    authors: Stamps AC,Davies SC,Burman J,O'Hare MJ

    更新日期:1994-06-15 00:00:00

  • Placental bone morphogenetic protein (PLAB) gene expression in normal, pre-malignant and malignant human prostate: relation to tumor development and progression.

    abstract::The second most common target of prostate-cancer metastasis is bone, and the phenomenon of skeletal metastasis represents the incurable stage of disease. Histologically, skeletal metastasis from prostate cancer is distinctive due to its osteoblastic nature. The osteoblastic bone metastasis shows extensive new bone for...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/ijc.1291

    authors: Thomas R,True LD,Lange PH,Vessella RL

    更新日期:2001-07-01 00:00:00

  • Human monoclonal antibody GNX-8 directed to extended type 1 chain: Specific binding to human colorectal cancer.

    abstract::We observed previously that two carbohydrate epitopes, extended type 1 chain Le(a)-Le(a) and Le(b)-Le(a), are expressed strongly in human gastric or colorectal cancer and cell lines derived therefrom, but their expression in human normal colorectal cells is highly limited. A monoclonal antibody, termed GNX-8, was esta...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/ijc.25131

    authors: Yang MC,Hsu FL,Handa K,Hakomori S,Lee MH,Liu LY,Chang SY,Ting J,Wen JY,Ishida I,Chang TH

    更新日期:2009-12-21 00:00:00

  • Ki-67 index enhances the prognostic accuracy of the urothelial superficial bladder carcinoma risk group classification.

    abstract::Approximately 80% of bladder tumors are urothelial superficial papillary carcinomas (USPC). Despite a generally good prognosis, these tumors have a strong propensity to recur and about 1/3 of them compared to disease progression. Histological assessment of these superficial tumors is not sufficiently discriminator in ...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/ijc.11049

    authors: Santos L,Amaro T,Costa C,Pereira S,Bento MJ,Lopes P,Oliveira J,Criado B,Lopes C

    更新日期:2003-06-10 00:00:00

  • Differential display analysis of breast carcinoma cells enriched by immunomagnetic target cell selection: gene expression profiles in bone marrow target cells.

    abstract::The red bone marrow (BM) is an important indicator organ of hematogenous micrometastatic spread of carcinomas. Characterization of biological properties specific for BM micrometastatic cells, however, is technically challenging due to the limited number of target cells usually available for the purpose. This report pr...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/ijc.1564

    authors: Ree AH,Engebraaten O,Hovig E,Fodstad Ø

    更新日期:2002-01-01 00:00:00

  • International osteosarcoma incidence patterns in children and adolescents, middle ages and elderly persons.

    abstract::Osteosarcoma incidence rates in the United States peak in adolescence and in the elderly. The international patterns of osteosarcoma incidence in children have been described, whereas those for young, middle age or elderly adults have not. Using the Cancer Incidence in Five Continents, International Agency for Cancer ...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/ijc.24320

    authors: Mirabello L,Troisi RJ,Savage SA

    更新日期:2009-07-01 00:00:00

  • Determination of the origin of oral squamous cell carcinoma by microarray analysis: Squamous epithelium or minor salivary gland?

    abstract::More than 90% of oral cancers are histopathologically squamous cell carcinomas (SCCs). According to clinical behavior and histopathological features, we hypothesize that oral SCC can originate from either oral squamous epithelium or minor salivary glands. Here, we examined whether some oral SCCs originate from minor s...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/ijc.31811

    authors: Kinouchi M,Izumi S,Nakashiro KI,Haruyama Y,Kobashi G,Uchida D,Hasegawa T,Kawamata H

    更新日期:2018-11-15 00:00:00

  • Investigation of downstream target genes of PAX3c, PAX3e and PAX3g isoforms in melanocytes by microarray analysis.

    abstract::PAX3 encodes a transcription factor, which with Zic1 is necessary for induction of the neural crest during early embryonic development. There are 7 human PAX3 isoforms (a-h). PAX3e is the full length isoform comprising 10 exons. PAX3c comprises 8 exons plus 5 codons of intron 8, while PAX3g has a truncated transactiva...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/ijc.22316

    authors: Wang Q,Kumar S,Mitsios N,Slevin M,Kumar P

    更新日期:2007-03-15 00:00:00

  • Identification of cisplatin-resistance related genes in head and neck squamous cell carcinoma.

    abstract::Resistance to cisplatin is a major obstacle to successful treatment of head and neck squamous cell carcinoma (HNSCC). To investigate the molecular mechanism of this resistance, we compared the gene expression profiles between the cisplatin-sensitive SCC cell lines (Sa-3, H-1 and KB) and the cisplatin-resistant cell li...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/ijc.24704

    authors: Yamano Y,Uzawa K,Saito K,Nakashima D,Kasamatsu A,Koike H,Kouzu Y,Shinozuka K,Nakatani K,Negoro K,Fujita S,Tanzawa H

    更新日期:2010-01-15 00:00:00

  • In vivo pH in metabolic-defective Ras-transformed fibroblast tumors: key role of the monocarboxylate transporter, MCT4, for inducing an alkaline intracellular pH.

    abstract::We present an investigation of tumor pH regulation, designed to support a new anticancer therapy concept that we had previously proposed. Our study uses a tumor model of ras-transformed hamster fibroblasts, CCL39, xenografted in the thighs of nude mice. We demonstrate, for the first time, that genetic modifications of...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/ijc.26125

    authors: Chiche J,Le Fur Y,Vilmen C,Frassineti F,Daniel L,Halestrap AP,Cozzone PJ,Pouysségur J,Lutz NW

    更新日期:2012-04-01 00:00:00

  • Inhibition of tumor growth by biodegradable microspheres containing all-trans-retinoic acid in a human head-and-neck cancer xenograft.

    abstract::Retinoids play essential roles in the regulation of cell differentiation and in the proliferation of various epithelial tissues, and atRA is one such active metabolite of retinoids. However, despite the known functions of atRA, its clinical applications are limited due to the induced metabolism by the specific cytochr...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/ijc.11354

    authors: Choi Y,Kim SY,Kim SH,Yang J,Park K,Byun Y

    更新日期:2003-10-20 00:00:00

  • FGF-2 isoforms of 18 and 22.5 kDa differentially modulate t-PA and PAI-1 expressions on the pancreatic carcinoma cells AR4-2J: consequences on cell spreading and invasion.

    abstract::Pancreatic tumors overexpress FGF-2 and t-PA, but the implication of the growth factor in t-PA synthesis and t-PA-dependent tumor invasion remains unknown. FGF-2 is present in different isoforms: The 18 kDa FGF-2 is secreted, while the 22.5 kDa one is nuclearized and exerts intracrine regulations bypassing cell-surfac...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/(sici)1097-0215(20000215)85:4<555::aid-ijc

    authors: Escaffit F,Estival A,Bertrand C,Vaysse N,Hollande E,Clemente F

    更新日期:2000-02-15 00:00:00

  • Endocrine sensitivity of the receptor-positive T61 human breast carcinoma serially grown in nude mice.

    abstract::A study was made on the effect of ovariectomy, 17 beta-oestradiol, and tamoxifen on the oestrogen and progesterone receptor-positive T61 human breast carcinoma grown in nude mice. The effect of the treatment was evaluated by the specific growth delay calculated on the basis of Gompertz growth curves, and by the change...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/ijc.2910350110

    authors: Brünner N,Spang-Thomsen M,Skovgaard Poulsen H,Engelholm SA,Nielsen A,Vindeløv L

    更新日期:1985-01-15 00:00:00

  • cDNA and protein characterization of human MAGE-10.

    abstract::MAGE genes are frequently expressed in several types of human malignancy and code for antigens recognized by cytotoxic T lymphocytes. We have previously described a monoclonal antibody (MAb), named 6C1, that recognizes the MAGE-1 protein and cross-reacts with a 72-kDa protein present in lysates of melanoma cells such ...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/(sici)1097-0215(19990909)82:6<901::aid-ijc

    authors: Rimoldi D,Salvi S,Reed D,Coulie P,Jongeneel VC,De Plaen E,Brasseur F,Rodriguez AM,Boon T,Cerottini JC

    更新日期:1999-09-09 00:00:00

  • Cell cycle-related kinase supports ovarian carcinoma cell proliferation via regulation of cyclin D1 and is a predictor of outcome in patients with ovarian carcinoma.

    abstract::Our previous study has suggested that the cell cycle-related kinase (CCRK) is a putative candidate oncogene in glioblastoma tumorigenesis. The potential oncogenic role of CCRK and its clinical/prognostic significance, however, in ovarian carcinoma are unclear. In this study, CCRK expression was examined by immunohisto...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/ijc.24630

    authors: Wu GQ,Xie D,Yang GF,Liao YJ,Mai SJ,Deng HX,Sze J,Guan XY,Zeng YX,Lin MC,Kung HF

    更新日期:2009-12-01 00:00:00

  • No excess risk for colorectal cancer among subjects seropositive for the JC polyomavirus.

    abstract::The human polyomaviruses JC virus (JCV) and BK virus (BKV) are oncogenic in experimental systems and commonly infect humans. JCV DNA has been reported to be present in human colon mucosa and in colorectal cancers. To investigate whether the risk for colorectal cancer is associated with JCV or BKV infection, we perform...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/ijc.22770

    authors: Lundstig A,Stattin P,Persson K,Sasnauskas K,Viscidi RP,Gislefoss RE,Dillner J

    更新日期:2007-09-01 00:00:00

  • Interferon induction in human mixed leukocyte-tumor-cell reactions: evidence for restriction to a certain lineage expressing glycophorin A.

    abstract::This study reports the induction of interferon (IFN) in human mononuclear cells (MNC) by hematopoietic tumor cells. Only 3 out of 15 cell lines were capable of inducing IFN in the mixed leukocyte/tumor-cell reaction (MLTR). K562, a pluripotent stem cell line and DUTKO-I, a hybrid between K562 and Daudi (Burkitt lympho...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/ijc.2910340108

    authors: Berger R,Knapp W,Kirchner H

    更新日期:1984-07-15 00:00:00

  • Establishment of a continuous tumor-cell line (panc-1) from a human carcinoma of the exocrine pancreas.

    abstract::An epithelioid cell line, started from a human pancreatic carcinoma of ductal cell origin, has been maintained in culture for over 2 years and has been subcultured more than 40 times. The PANC-1 cell line has a doubling time of 52 h and G6PD activity of the slow mobility of B type. Chromosome studies show a modal numb...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/ijc.2910150505

    authors: Lieber M,Mazzetta J,Nelson-Rees W,Kaplan M,Todaro G

    更新日期:1975-05-15 00:00:00

  • GD2-directed CAR-T cells in combination with HGF-targeted neutralizing antibody (AMG102) prevent primary tumor growth and metastasis in Ewing sarcoma.

    abstract::Ewing sarcoma (EWS) is the second most common and aggressive type of metastatic bone tumor in adolescents and young adults. There is unmet medical need to develop and test novel pharmacological targets and novel therapies to treat EWS. Here, we found that EWS expresses high levels of a p53 isoform, delta133p53. We fur...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/ijc.32743

    authors: Charan M,Dravid P,Cam M,Audino A,Gross AC,Arnold MA,Roberts RD,Cripe TP,Pertsemlidis A,Houghton PJ,Cam H

    更新日期:2020-06-01 00:00:00

  • Risk of somatic diseases in offspring of survivors with childhood or adolescent central nervous system tumor in Sweden.

    abstract::With the improvement of treatments, a growing number of survivors with childhood or adolescent central nervous system (CNS) tumor are parenting their own children. We aimed to explore the risk of somatic diseases among children of these survivors compared to population controls. Children of survivors with CNS tumor be...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/ijc.33394

    authors: Huang W,Sundquist K,Sundquist J,Ji J

    更新日期:2020-11-13 00:00:00

  • HER2/neu over-expression induces endothelial cell retraction.

    abstract::Over-expression of the HER2/neu (HER2) proto-oncogene in breast carcinoma imparts an enhanced metastatic potential. Metastasis requires escape of the tumor cell from the vasculature into subjacent tissue, a transmigration event across an endothelial cell (EC) monolayer. EC retraction has been reported to precede trans...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/1097-0215(200002)9999:9999<::aid-ijc1061>3

    authors: Carter WB,Hoying JB,Boswell C,Williams SK

    更新日期:2001-02-01 00:00:00

  • Canine testicular tumors: epidemiologic features of 410 dogs.

    abstract::Histologically confirmed testicular tumors were diagnosed in 410 dogs from 12 North American veterinary university hospitals and clinics. Three tumor-cell types, Sertoli cell tumor, interstitial cell tumor and seminoma, were about equally represented. Several breeds were identified with high risk for different testicu...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/ijc.2910180413

    authors: Hayes HM Jr,Pendergrass TW

    更新日期:1976-10-15 00:00:00

  • Suppression of cyclooxygenase-2 overexpression by 15S-hydroxyeicosatrienoic acid in androgen-dependent prostatic adenocarcinoma cells.

    abstract::Emerging reports now implicate alterations of arachidonic acid (AA) metabolism with prostate carcinogenesis. To test this hypothesis, androgen-primed benign hyperplastic (BHC) and malignant tumorigenic (MTC) cells derived from the Lobund-Wistar rat model of autochthonous prostate adenocarcinoma were incubated with (14...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/ijc.20245

    authors: Pham H,Banerjee T,Ziboh VA

    更新日期:2004-08-20 00:00:00

  • Drug-regulatable cancer cell death induced by BID under control of the tissue-specific, lung cancer-targeted TTS promoter system.

    abstract::Gene therapy and virotherapy are among the approaches currently being used to treat lung cancer. The success of cancer gene therapy depends on treatments where different types of tumors can be selectively targeted and destroyed without affecting normal cells and tissue. Previously, we described a promoter system (TTS)...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/ijc.24584

    authors: Fukazawa T,Maeda Y,Matsuoka J,Tanaka N,Tanaka H,Durbin ML,Naomoto Y

    更新日期:2009-10-15 00:00:00

  • Elevated expression of eIF4E in confined early breast cancer lesions: possible role of hypoxia.

    abstract::The translation-initiation factor eIF4E is rate-limiting for protein synthesis, and its over-expression results in oncogenic transformation of mammalian cells. eIF4E facilitates the synthesis of several powerful tumor angiogenic factors (FGF-2 and VEGF) by selectively enhancing their translation. In breast carcinomas,...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/(sici)1097-0215(19990209)80:4<516::aid-ijc

    authors: DeFatta RJ,Turbat-Herrera EA,Li BD,Anderson W,De Benedetti A

    更新日期:1999-02-09 00:00:00

  • Prognostic and diagnostic potential of local and circulating levels of pentraxin 3 in lung cancer patients.

    abstract::There is a well-established link between inflammation and cancer of various organs, but little data are available on inflammation-associated markers of diagnostic and prognostic clinical utility in pulmonary malignancy. Blood samples were prospectively collected from 75 resectable lung cancer patients before surgery a...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/ijc.29822

    authors: Infante M,Allavena P,Garlanda C,Nebuloni M,Morenghi E,Rahal D,Roncalli M,Cavuto S,Pesce S,Monari M,Valaperta S,Montanelli A,Solomon D,Bottoni E,Errico V,Voulaz E,Bossi M,Chiesa G,Passera E,Mantovani A,Alloisio M

    更新日期:2016-02-15 00:00:00

  • Analysis of the p53 gene in relation to tobacco and alcohol in cancers of the upper aero-digestive tract.

    abstract::To elucidate the relationship, if any, of p53 gene expression according to smoking and drinking habits, 135 subjects with cancer of the upper aero-digestive tract were sampled from a case-control investigation conducted in Pordenone province, Northeast Italy, between 1986 and 1991. Adequate pathological material for i...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/ijc.2910600625

    authors: Franceschi S,Gloghini A,Maestro R,Barzan L,Bidoli E,Talamini R,Vukosavljevic T,Carbone A,Boiocchi M

    更新日期:1995-03-16 00:00:00

  • Maternal and perinatal factors associated with non-Hodgkin's lymphoma among children.

    abstract::This nested case-control study based on 1.7 million live births in Sweden explores the associations between maternal and perinatal factors and the occurrence of childhood non-Hodgkin's lymphoma (NHL). The National Swedish Cancer Registry ascertained 168 cases in successive birth cohorts from 1973 through 1989 recorded...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-0215(19960315)65:6<774::AID-IJC

    authors: Adami J,Glimelius B,Cnattingius S,Ekbom A,Zahm SH,Linet M,Zack M

    更新日期:1996-03-15 00:00:00