Abstract:
:The incidence of noroviral gastroenteritis has increased dramatically in recent years, and norovirus (NoV) genogroup II.4 (GII.4) is associated with outbreaks worldwide. The NoV genotypes and their clinical relevance in children hospitalized with acute gastroenteritis between 2006 and 2011 in northern Taiwan were evaluated in this study. NoV sequences were amplified from 47 clinical specimens and phylogenetic analysis was performed. Based on noroviral capsid protein (VP1) and RNA dependent RNA polymerase (RdRp) phylogeny, circulating NoV could be divided into GII.2, GII.3, GII.12, and GII.4 and GII.16, GII.12, GII.g, and GII.4; respectively. The GII.4 subtype was predominant and could be divided further into the 2004 (Hunter), 2006b, and 2010 (New Orleans) subtypes. Regarding clinical manifestations, convulsive disorder occurred only in cases caused by NoV GII.4 2006b. Patients affected by NoV GII.4 2006b presented with a higher frequency of diarrhea (P = 0.0204), longer duration of diarrhea (P = 0.0215), more frequent hypoglycemia (P = 0.038), and electrolyte imbalance (P = 0.0487) than acute gastroenteritis caused by NoV GII.4 2010. Structural analysis showed that the amino acid changes in viral VP1 between GII.4 2006b and 2010 subtype were located mainly in the protruding domain 2 (P2 domain). In conclusion, the NoV GII.4 variants 2006b and 2010 were the main causes of acute gastroenteritis in hospitalized children in northern Taiwan during 2006-2011. The clinical presentations and structural changes in VP1 of the two NoV GII.4 variants should be evaluated in the future.
journal_name
J Med Viroljournal_title
Journal of medical virologyauthors
Tsai CN,Lin CY,Lin CW,Shih KC,Chiu CH,Chen SYdoi
10.1002/jmv.23728subject
Has Abstractpub_date
2014-02-01 00:00:00pages
335-46issue
2eissn
0146-6615issn
1096-9071journal_volume
86pub_type
杂志文章abstract::Coronavirus disease 2019 (COVID-19) can lead to a massive cytokine release. The use of the anti-interleukin-6 receptor monoclonal antibody tocilizumab (TCZ) has been proposed in this hyperinflammatory phase, although supporting evidence is limited. We retrospectively analyzed 88 consecutive patients with COVID-19 pneu...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.26308
更新日期:2021-02-01 00:00:00
abstract::It have been suggested that hepatitis B virus (HBV) pre-S deletions may play a role in hepatocarcinogenesis. The aim of the study was to determine the prevalence of pre-S deletions in chronic hepatitis B patients in Hong Kong, the factors associated with the deletions and its relationship with hepatitis B e antigen (H...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21901
更新日期:2010-11-01 00:00:00
abstract::Heterogeneity of hepatitis C viral (HCV) genomes of several isolates from different countries has been reported, but there is little information on HCV isolates for the Federal Republic of Germany. Therefore, the nucleotide (nt) and deduced amino acid (aa) sequences of interesting parts of the viral genome derived fro...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890400407
更新日期:1993-08-01 00:00:00
abstract::Rotaviruses were detected via electron microscopy in fecal specimens collected from school children during an outbreak of diarrhea and from a sporadic case in 1993 in Japan. All of the viruses were found to belong to human group C rotavirus by reverse passive hemagglutination assay (RPHA). These viruses replicated wel...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/(SICI)1096-9071(199601)48:1<48::AID-JMV8>3
更新日期:1996-01-01 00:00:00
abstract::The prevalence study and the characterization of hepatitis C virus (HCV) was carried out in the Philippines and the sequence determination of the 5'-untranslated region (5'-UTR)-Core and the NS5B regions of HCV was carried out in this study. An HCV strain (SE-03-07-1689) collected in Metro Manila, Philippines, belonge...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20714
更新日期:2006-11-01 00:00:00
abstract::Hepatitis C virus core antigen (HCVcoreAg) may be measured in serum with a sensitive, recently validated assay. Beyond its value as a marker of viral infection, there are little data on its relation with clinical, histological, and virological parameters. In this study, the significance of HCVcoreAg levels was studied...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23672
更新日期:2013-11-01 00:00:00
abstract::Rotavirus was detected in the stools of five children stricken with sudden infant death syndrome (SIDS) over a three-week period. While none of the children had acute gastroenteritis, four of the five had acute upper respiratory infections. Rotavirus was also identified in tracheal aspirates from two of the infants. E...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890100409
更新日期:1982-01-01 00:00:00
abstract::Hepatitis D virus (HDV) infection can cause severe acute and chronic liver disease in patients infected with hepatitis B virus (HBV). Despite the significant decline in the global HDV infection, it remains a major health concern in some countries. This study aimed to investigate the prevalence and clinical features of...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.22095
更新日期:2011-07-01 00:00:00
abstract::Recently a conventional method for the laboratory diagnosis of human cytomegalovirus (HCMV) infection was improved by using centrifugation culture to enhance viral adsorption and by detecting HCMV early antigen and DNA. Comparison of the sensitivity of three rapid methods using commercial diagnostic reagents for the d...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890300204
更新日期:1990-02-01 00:00:00
abstract::A seroprevalence study was carried out of six different human pathogenic viruses, namely human immunodeficiency virus (HIV), hepatitis B virus (HBV), hepatitis C virus (HCV), human T-cell leukemia virus (HTLV), human herpesvirus type 8 (HHV-8), and dengue virus among pregnant women and blood donors from rural (Nouna) ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20593
更新日期:2006-05-01 00:00:00
abstract::It remains unclear if China's current HIV antibody testing algorithm misses a substantial number of HIV infected individuals. Of 196 specimens with indeterminate or negative results on HIV western blot (WB) retrospectively examined by HIV-1 nucleic acid test (NAT), 67.57% (75/111) of indeterminate WB samples, and 16.4...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24490
更新日期:2016-08-01 00:00:00
abstract::Lymphocytic choriomeningitis virus (LCMV), which is one of several arenaviruses that are pathogenic for humans, causes encephalitis and meningitis in man. In this study, single-stage and nested reverse transcription-polymerase chain reaction (RT-PCR) assays were developed that targeted the GPC and N genes of LCMV. Bot...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:
更新日期:1997-02-01 00:00:00
abstract::Herpes simplex virus (HSV) infection of the corneal stroma is the most prominent cause of scar formation impairing visual acuity and HSV keratitis is the leading cause of corneal opacity throughout the world. Suriname lacked test systems for microbial causes of ocular disease, therefore a polymerase chain reaction-bas...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23414
更新日期:2012-12-01 00:00:00
abstract::To characterize cytotoxic T lymphocytes (CTLs) that appeared in circulation during lamivudine therapy, we analyzed HBV-specific CTLs using HLA-A24 tetramer and HBcAg-specific Th1 cells in patients receiving lamivudine therapy. Six patients (HLA-A24(+)) with chronic hepatitis B, six patients (HLA-A24(-)) with chronic h...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20194
更新日期:2004-11-01 00:00:00
abstract::KI is a novel polyomavirus identified in the respiratory secretions of children with acute respiratory symptoms. Whether this reflects a causal role of the virus in the human respiratory disease remains to be established. To investigate the presence of KIV in the respiratory tissue, we examined 20 fresh lung cancer sp...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21417
更新日期:2009-03-01 00:00:00
abstract::HIV-1 replicative activity and its relation to the clinical and immunological evolution of infection was studied in a group of 150 HIV-1 seropositive Italian i.v. drug abusers over a 1 year period. HIV-1 was isolated from 90 (60%) subjects; two groups of isolates were distinguished, according to replicative activity "...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890320411
更新日期:1990-12-01 00:00:00
abstract::Enterovirus infections are usually mild but can also cause severe illnesses and play a role in chronic diseases, such as cardiomyopathies and type 1 diabetes. Host response to the invading virus can markedly modulate the course of the infection, and this response varies between individuals due to the polymorphism of i...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23903
更新日期:2014-08-01 00:00:00
abstract::Monoclonal antibodies were raised against hepatitis B core antigen (HBcAg) synthesized in Escherichia coli. They identified a single immunodominant determinant on both liver-derived and E. coli-derived HBcAg. Cross-inhibition studies demonstrated that HBsAg-containing human sera which contained antibodies to HBcAg (an...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890190213
更新日期:1986-06-01 00:00:00
abstract::Glycoproteins of enveloped viruses replicating in nonprimate mammalian cells carry α-1,3-galactose (α-Gal) glycans, and can bind to anti-Gal antibodies which are abundant in humans. The antibodies have protected humans and their ancestors for millions of years, because they inhibit replication of many kinds of microbe...
journal_title:Journal of medical virology
pub_type: 杂志文章,评审
doi:10.1002/jmv.26312
更新日期:2020-07-18 00:00:00
abstract::The effect of antiviral treatment on apoptosis in chronic hepatitis B (CHB) has not been clarified. We evaluated the hepatic immunohistochemical expression of the pro-apoptotic bax and the antiapoptotic bcl-xL protein in HBeAg-negative CHB patients before and after treatment. In our study we included 72 paired biopsie...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21780
更新日期:2011-07-01 00:00:00
abstract::Management of patients infected with high-risk HPV (hrHPV) despite normal colposcopy following abnormal cytology remains a clinical challenge. The aim of this study was to evaluate if, in that specific population, initial HPV 16 and HPV 18 viral loads are predictive of infection clearance over a 24-month follow-up. A ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23276
更新日期:2012-06-01 00:00:00
abstract::A small percentage of renal patients become infected with the BK polyomavirus (BKV), a pathogenic virus that causes BKV-associated nephropathy (BKVN), after kidney transplantation. This study presents a simple, rapid, high-throughput method for BKV genotyping using high-resolution melt analysis (HRMA). Using this nove...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.22239
更新日期:2011-12-01 00:00:00
abstract::Herpes simplex virus can be quantitatively recovered from vaginal tampons suspended in tissue culture medium and stored over a wide range of temperatures. Because herpes simplex virus is stable for several days with refrigeration or after freezing, this method of culture of cervicovaginal virus lends itself to epidemi...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890030306
更新日期:1979-01-01 00:00:00
abstract::There is little information on JC virus (JCV) infection in renal transplant patients. A long-term prospective follow-up study was conducted to assess the incidence of JCV DNA in the blood of 103 adult renal transplant patients enrolled prospectively between 1 January and 31 December 2006. Patients were monitored until...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21951
更新日期:2011-01-01 00:00:00
abstract::Although neutralizing antibodies against Hantaan virus (HTV) can protect hosts from viral infection, T-cell responses to HTV are also important in host defense against HTV. However, much less is known about cytotoxic T lymphocyte (CTL) responses to HTV. To identify CTL epitopes in the HTV nucleocapsid protein (NP), we...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:
更新日期:2000-02-01 00:00:00
abstract::Most dengue virus (DENV) infections remain asymptomatic. This increases the risk of DENV transfusion transmission (TT-DENV) during outbreaks. We evaluated DENV viremia in 8475 blood donations assembled in minipools for the presence of DENV RNA. The tested samples were obtained between February and May, 2016, during a ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.26619
更新日期:2020-10-22 00:00:00
abstract::Knowledge of the nucleotide sequence in the region of the putative VP1/2A junction of the Hepatitis A virus (HAV) genome has enabled differentiation of HAV strains and their classification into seven genotypes, in some of which sub-genotypes A and B can be defined. A 168 base segment encompassing the putative VP1/2A j...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1023
更新日期:2001-06-01 00:00:00
abstract::Clinical, virological, and histological findings in four HBsAg chronic carriers who cleared HBV markers from both serum and liver following HDV superinfection are described. The patients were long-term HBsAg carriers and all were HBV-DNA/HBeAg positive. Liver biopsy, obtained from three of the patients between 5 and 1...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890310408
更新日期:1990-08-01 00:00:00
abstract::Influenza A(H1N1)pdm09 virus pandemic struck India in 2009 and continues to cause outbreaks in its post-pandemic phase. Diminutive information is available about influenza A(H1N1)pdm09 from central India. This observational study presents epidemiological and molecular findings for the period of 6 years. Throat swab sa...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24982
更新日期:2018-03-01 00:00:00
abstract::Human metapneumovirus, with two known genotypes named A and B, is associated with mild respiratory symptoms to severe LRTI in children, high-risk adults and the elderly. Rapid and reliable methods of hMPV detection in clinical samples are essential to implement appropriate care, to better understand the pathology of h...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21038
更新日期:2008-01-01 00:00:00