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Antitumoural effect of Synadenium grantii Hook f. (Euphorbiaceae) latex.

Abstract：

ETHNOPHARMACOLOGICAL RELEVANCE:Synadenium grantii Hook f. has traditionally been used to treat various neoplastic diseases in southern Brazil. AIM OF STUDY:Evaluation of the antitumoural potential of Synadenium grantii latex against B16F10 melanoma cell line using in vitro and in vivo models, as well as a phytochemical study of the latex. MATERIALS AND METHODS:The in vitro antitumoural activity was performed using MTT and trypan blue assays with different latex concentrations (1.7 µg-7.0 µg/well and 1.22 mg-4.88 mg/well). Flow cytometry was used to determine the progression of the cell cycle. The in vivo activity was performed by subcutaneously injecting melanoma cells in the dorsum of C57BL6 mice, followed by treating the mice with a popular form of use of the latex (garrafada) administered orally. After sacrificing the animals, histological analysis of the organs was performed by hematoxylin-eosin staining. The phytochemical study of the latex was performed by NMR and chromatographic procedures and the extracts and isolated substances were evaluated by IR, 1D and 2D NMR analysis. RESULTS:The Synadenium grantii latex exhibited decreased cell viability of the melanoma line in a concentration and time-dependent manner, and also cell cycle arrest in the S-G2/M phase. The latex caused a 40% reduction in the volume of tumours of the mice with melanomas. Histological examination of the organs of these animals showed no differences between groups. The phytochemical investigation resulted in the isolation and identification of triterpene euphol and the steroid citrostadienol, which were tested against the strain of melanoma. Euphol showed no antitumoural activity, while the steroid citrostadienol showed reduced cytotoxic activity. CONCLUSION:The Synadenium grantii latex presented in vitro and in vivo cytotoxic effects with antitumoural activity against B16F10 melanoma cells.

journal_name

J Ethnopharmacol

journal_title

Journal of ethnopharmacology

authors

de Oliveira TL,Munhoz AC,Lemes BM,Minozzo BR,Nepel A,Barison A,Fávero GM,Campagnoli EB,Beltrame FL

doi

10.1016/j.jep.2013.08.033

subject

Has Abstract

pub_date

2013-10-28 00:00:00

pages

263-9

issue

eissn

0378-8741

issn

1872-7573

pii

S0378-8741(13)00587-4

journal_volume

150

pub_type

杂志文章

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Antitumoural effect of Synadenium grantii Hook f. (Euphorbiaceae) latex.