Abstract:
AIM OF THE STUDY:Huang-Lian-Jie-Du-Tang (HLJDT) is a traditional Chinese medicine with anti-inflammatory use. In the present study, the effects of its component herbs and pure components were observed on eicosanoid generation to find out the contributory components and their precise targets on arachidonic acid (AA) cascade. MATERIALS AND METHODS:By monitoring leukotriene B(4) (LTB(4)), 5-hydroxyeicosatetraenoic acid (5-HETE), and 12-hydroxy-5,8,10-heptadecatrienoic acid (12-HHT), we compared the effects of HLJDT, HLJDT free of one or two component herbs, and water extract of four single component herbs of HLJDT (Rhizoma coptidis, Radix scutellariae, Cortex phellodendri and Fructus gardeniae) on eicosanoid generation in rat elicited peritoneal macrophages. In addition, thirteen pure compounds from HLJDT (baicalin, baicalein, wogonoside, wogonin, berberine, magnoflorine, phellodendrine, coptisine, palmatine, jateorrhizine, crocin, chlorogenic acid, and geniposide) were tested in the macrophages. Furthermore, the efficacies of these thirteen compounds were evaluated on cell-free purified enzymes: leukotriene A(4) hydrolase (LTA(4)H), 5-, 15-lipoxygenase (5-, 15-LO), and cyclo-oxygenase-1/2 (COX-1/2). Moreover, the possible synergetic effect on LO pathway derived LTB(4) generation between the active components was also tested in rat peritoneal macrophages. RESULTS:Our experiments showed that Rhizoma coptidis and Radix scutellariae were responsible for the suppressive effect of HLJDT on eicosanoid generation. Some of the pure components including baicalein, baicalin, wogonoside, wogonin, coptisine, and magnoflorine inhibited eicosanoid generation in rat macrophages via LO pathway of AA cascade. Further experiments on cell-free purified enzymes confirmed that Radix scutellariae derived baicalein and baicalin showed significant inhibition on 5-LO and 15-LO, while Rhizoma coptidis derived coptisine showed medium inhibition on LTA(4)H. On the other hand, no significant inhibition of thirteen components on COX-1/2 was observed. Moreover, the slight synergetic inhibition on LTB(4) between baicalein and coptisine was proved in the rat peritoneal macrophages. CONCLUSIONS:Baicalein and coptisine, the active components of HLJDT, for the first time are found to interfere with arachidonic acid cascade via inhibition on different points of LO pathway. This finding makes the mechanism of HLJDT clearer and achieves its safer therapeutic application.
journal_name
J Ethnopharmacoljournal_title
Journal of ethnopharmacologyauthors
Zeng H,Dou S,Zhao J,Fan S,Yuan X,Zhu S,Li L,Zhang W,Liu Rdoi
10.1016/j.jep.2011.03.055subject
Has Abstractpub_date
2011-05-17 00:00:00pages
561-8issue
2eissn
0378-8741issn
1872-7573pii
S0378-8741(11)00206-6journal_volume
135pub_type
杂志文章abstract::Twelve of 74 ethanolic extracts of plants used by traditional healers for snakebites in the northwest region of Colombia, were active against lethal effect of Bothrops atrox venom when they were i.p. injected into mice (18-20 g). After preincubation of sublethal doses of every extract (0.5-4.0 mg/mouse) with 1.5 i.p. ...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/s0378-8741(99)00197-x
更新日期:2000-08-01 00:00:00
abstract::Paeoniflorin and sinomenine, derived from the root of Paeonia lactiflora Pall. (family Ranunculaceae) and the stem of Sinomenium acutum Rehder & Wilson (family Menispermaceae), respectively, have been, and are currently, widely used for treatment of rheumatic and arthritic diseases in China and Japan. Our previous stu...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2005.08.020
更新日期:2006-02-20 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:The ethnic Chinese Yao medicine Baizuan, which are the leaves of Schisandra viridis A. C. Smith, is traditionally used, in combination with other herbs, to soften hard lumps and dispel nodes in the treatment of cancer, however, this property has not been well studied with a clear indicati...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2016.09.016
更新日期:2016-12-24 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Ginkgo biloba L. (Ginkgoaceae) has been widely used in traditional medicine for variety of neurological conditions particularly behavioral and memory impairments. AIM OF THE STUDY:The present study was envisaged to explore the effect of a standardized fraction of Ginkgo biloba leaves (GB...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2017.03.060
更新日期:2017-05-23 00:00:00
abstract::The antiinflammatory (per os and topic) and analgesic (per os) properties of the aerial part of Proustia pyrifolia a species in danger of extinction were investigated, and the major compounds of two of its active extracts were isolated. In addition, the evaluation of cytotoxicity in three tumoral cell lines and the ac...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2005.02.012
更新日期:2005-05-13 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Traditional Chinese Medicine (TCM) has many obvious advantages in the treatment of chronic conditions such as urinary tract infection (UTI). Dongbai-Tonglin-Fang (DBTL), a Chinese herbal formula, has been used for the treatment of UTI for more than 40 years with proven efficacy. However, ...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2019.112028
更新日期:2019-09-15 00:00:00
abstract::Aqueous EtOH (80%) extracts of seven plants used by Rwandan traditional healers to treat infections, were screened for antibacterial, antifungal, and antiviral activities. Only two of the selected plants showed a true antiviral activity against herpes simplex virus type 1, while all of them exhibited virucidal propert...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/s0378-8741(98)00154-8
更新日期:1999-04-01 00:00:00
abstract:AIM OF THE STUDY:In African traditional medicine, different parts of Phyllanthus amarus Schum. and Thonn. (family: Euphorbiaceae) are highly valued for the treatment of array of human diseases including hepatic and urolithic and/or other renal diseases. In the present study, single oral 100-400mg/kg/day of the leaf and...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2008.04.025
更新日期:2008-07-23 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Tecoma stans aqueous extract (TAE) is widely used as a traditional antidiabetic remedy in Mexico; its rational use is controversial. We provide evidence of its main antidiabetic activities. AIM OF THE STUDY:To evaluate in vivo and in vitro intestinal alpha-glucosidases inhibition as the ...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2009.04.033
更新日期:2009-07-15 00:00:00
abstract:UNLABELLED:ETHNOPHAMACOLOGICAL RELEVANCY: The whole plant of Talinum triangulare (Family: Portulacaceae) is used in variety of diseases including hepatic ailments in Africa and Taiwan of China. AIMS OF THE STUDY:The study was aimed to evaluate the antioxidant and hepatoprotective activity of polysaccharides from T. tr...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2011.04.047
更新日期:2011-06-22 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Traditional medical systems represent an important source of knowledge about the use of biodiversity. Ethnobotanical and ethnopharmacological studies can encourage bioprospecting in the pursuit and selection of promising medicinal plants. This study investigated the diversity of medicinal...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2014.07.042
更新日期:2014-09-29 00:00:00
abstract:AIM OF THE STUDY:The present study was undertaken to evaluate the wound healing effects of the four chief saponins of Astragalus species [cycloastragenol (CA), astragaloside IV (AG), cyclocephaloside I (CCI) and cyclocanthoside E (CCE)]. MATERIAL AND METHODS:Effects of cell viability and proliferation of the isolated ...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2011.01.030
更新日期:2011-04-12 00:00:00
abstract::Bioassay-guided fractionation of the rhizomes of Astilbe chinensis afforded four cytotoxic pentacyclic triterpenoids, 3beta-hydroxy-olean-12-en-27-oic acid (1), 3beta,6beta-dihydroxy-olean-12-en-27-oic acid (2), 3beta-acetoxy-olean-12-en-27-oic acid (3), and 3beta-hydroxy-urs-12-en-27-oic acid (4). The isolation and p...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2003.10.003
更新日期:2004-02-01 00:00:00
abstract::We evaluated the possible scavenging effects of Aloe arborescens Miller var. natalensis Berger (Kidachi aloe in Japanese) on free radicals generated by streptozotocin (Sz) or alloxan (Ax). The components of Kidachi aloe were added to a reaction system in which .OH radicals derived from Sz or Ax as pancreatic islet B-c...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/s0378-8741(03)00268-x
更新日期:2003-11-01 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Substantially, plants produce chemicals such as primary and secondary metabolites, which have significant applications in modern therapy. Indigenous people mostly rely on traditional medicines derived from medicinal plants. These plants have the capacity to absorb a variety of toxic eleme...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章,评审
doi:10.1016/j.jep.2016.05.011
更新日期:2016-07-21 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Chyawanprash (CP), a traditional immune booster recipe, has a long history of ethnic origin, development, household preparation and usage. There are even mythological stories about the origin of this recipe including its nomenclature. In the last six decades, CP, because of entrepreneuria...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章,评审
doi:10.1016/j.jep.2016.07.078
更新日期:2017-02-02 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Boesenbergia rotunda (L) Mansf. has been used for the treatment of gastrointestinal disorders including peptic ulcer. In the current study we aimed to investiagte the anti-ulcer activities of methanolic extract of B. rotunda (MEBR) and its main active compound, pinostrobin on ethanol-indu...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2011.07.010
更新日期:2011-09-02 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Turmeric (Curcuma longa) has traditionally been used to treat pain, fever, allergic and inflammatory diseases such as bronchitis, arthritis, and dermatitis. In particular, turmeric and its active component, curcumin, were effective in ameliorating immune disorders including allergies. How...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2015.08.038
更新日期:2015-12-04 00:00:00
abstract:AIM OF THE STUDY:Calotropis procera is a wild growing plant with multifarious medicinal properties. The present study was carried out to evaluate the effect of dried latex (DL) of Calotropis procera and its methanol extract (MeDL) against gastric ulcers induced in rats. MATERIALS AND METHODS:Aqueous suspension of DL (...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2009.10.016
更新日期:2010-02-03 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Dahuang Fuzi Decoction (DFD) is a traditional well-prescribed formula for the treatment of chronic kidney disease (CKD) in China. This study was carried out to examine the effects of DFD in adenine-induced tubular epithelial apoptosis and renal damage, in comparison with allopurinol (AP),...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2014.08.035
更新日期:2014-10-28 00:00:00
abstract::The effects of cryptolepine alone and in combination with other antiplatelet agents have been investigated using a mouse model of arterial thrombosis. Intraperitoneal premedication with crytolepine produced 25% maximal protection at 1 mg/kg while dipyridamole producedd a 20% maximal effect at 2 mg/kg. Higher doses of ...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/0378-8741(89)90086-x
更新日期:1989-11-01 00:00:00
abstract:BACKGROUND AND PURPOSE:Based on ethno-botanical information collected from diabetic patients in Cuba and firstly reported inhibition of PTP1B and DPPIV enzymes activities, Allophylus cominia (A. cominia) was identified as possible source of new drugs that could be used for the treatment of type 2 diabetes mellitus (T2-...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2018.01.014
更新日期:2018-04-24 00:00:00
abstract::Melissa officinalis L. (Labiatae), Morus alba L. (Moraceae), and Artemisia capillaris Thunb. (Compositae) are suggested to be involved in the regulation of hyperlipidemia. We hypothesized that Ob-X, a mixture of three herbs, Morus alba, Melissa officinalis and Artemisia capillaris [corrected] improves lipid metabolism...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2007.09.029
更新日期:2008-01-17 00:00:00
abstract:AIM OF THE STUDY:Experiments were conducted to understand the therapeutic properties such as anti-inflammatory and anti-pyretic activities of biologically active extract isolated from whole earthworm (Lampito mauritii, Kinberg). MATERIALS AND METHODS:Inflammation in the hind paw of Wistar albino rat, Rattus norvegicus...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2008.10.021
更新日期:2009-01-21 00:00:00
abstract::The anticonvulsant effect of the volatile oil extracted from the fresh fruits of Tetrapleura tetraptera was investigated in mice. The results obtained show that the fresh oil given intraperitoneally offers some protection against leptazol-induced convulsions. A dose of 0.4 ml of the oil per mouse protected 78% of the ...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/0378-8741(86)90023-1
更新日期:1986-11-01 00:00:00
abstract:AIM OF THE STUDY:Dangnyohwan (DNH) has been used for treatment of diabetes mellitus. However, the exact cellular and molecular mechanisms underlying the beneficial effects of DNH are not well understood. Therefore, we investigated how DNH improves hyperglycemia and insulin resistance in obese-type diabetes model. METH...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2007.10.040
更新日期:2008-02-12 00:00:00
abstract::Traditional livestock healers from Samburu and Turkana communities in Samburu District of Kenya were identified and recruited for participation in this study. Sheep with mixed gastrointestinal helminthosis were purchased from nomadic livestock owners in the area. Three herbal anthelmintic remedies, viz. Myrsine africa...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2003.11.007
更新日期:2004-03-01 00:00:00
abstract::With cancer deaths increasing, the initiation, pathophysiology and curative management of cancer is receiving increasing attention. Traditional therapies such as surgery and chemoradiotherapy are often accompanied by suppression of host immunity, which increase the risk of metastasis. Astragalus membranceus (AM) is co...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章,评审
doi:10.1016/j.jep.2020.112797
更新日期:2020-08-10 00:00:00
abstract:AIM OF THE STUDY:Around the world, Tilia species have been used in traditional medicine for their properties as tranquilizer. Furthermore, Mexican species of Tilia have been grouped as Tilia americana var. mexicana, but their specific content in flavonoids is poorly described. In this study, inflorescences of Mexican T...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2009.09.044
更新日期:2010-01-08 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Fructus Arctii is the dried ripe fruit of Arctium lappa L. (family Asteraceae). It is a well-known Chinese Materia Medica that was included in the Chinese pharmacopoeia because of its traditional therapeutic actions, such as heat removal, detoxification, and elimination of swelling. Since...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2020.112773
更新日期:2020-06-12 00:00:00