VX-809 corrects folding defects in cystic fibrosis transmembrane conductance regulator protein through action on membrane-spanning domain 1.

abstract：:Functional proteins of complex eukaryotes within the same species are rather invariant. A single catalytic component of telomerase TERT is essential for an active telomerase complex that maintains telomeres. Surprisingly, we have identified two paralogous SpTERT-L and SpTERT-S genes with novel domains in Strongylocent...

journal_title：Molecular biology of the cell

authors： Wells TB,Zhang G,Harley Z,Vaziri H

更新日期：2009-01-01 00:00:00

abstract：:Microtubules (MTs) have been proposed to act mechanically as compressive struts that resist both actomyosin contractile forces and their own polymerization forces to mechanically stabilize cell shape. To identify the origin of MT bending, we directly observed MT bending and F-actin transport dynamics in the periphery ...

journal_title：Molecular biology of the cell

authors： Bicek AD,Tüzel E,Demtchouk A,Uppalapati M,Hancock WO,Kroll DM,Odde DJ

更新日期：2009-06-01 00:00:00

abstract：:Protein phosphatase 1 (PP1) is a ubiquitous serine/threonine phosphatase that regulates many cellular processes, including cell division. When transiently expressed as fluorescent protein (FP) fusions, the three PP1 isoforms, alpha, beta/delta, and gamma1, are active phosphatases with distinct localization patterns. W...

journal_title：Molecular biology of the cell

authors： Trinkle-Mulcahy L,Andrews PD,Wickramasinghe S,Sleeman J,Prescott A,Lam YW,Lyon C,Swedlow JR,Lamond AI

更新日期：2003-01-01 00:00:00

abstract：:Rapid alteration of gene expression in response to environmental changes is essential for normal development and behavior. The transcription factor hypoxia-inducible factor (HIF)-1 is well known to respond to alterations in oxygen availability. In nature, low oxygen environments are often found to contain high levels ...

journal_title：Molecular biology of the cell

authors： Budde MW,Roth MB

更新日期：2010-01-01 00:00:00

abstract：:The cytoskeleton plays an important role in neuronal morphogenesis. We have identified and characterized a novel actin-binding protein, termed Mayven, predominantly expressed in brain. Mayven contains a BTB (broad complex, tramtrack, bric-a-brac)/POZ (poxvirus, zinc finger) domain-like structure in the predicted N ter...

journal_title：Molecular biology of the cell

authors： Soltysik-Espanola M,Rogers RA,Jiang S,Kim TA,Gaedigk R,White RA,Avraham H,Avraham S

更新日期：1999-07-01 00:00:00

abstract：:Adaptins are subunits of adaptor protein (AP) complexes involved in the formation of intracellular transport vesicles and in the selection of cargo for incorporation into the vesicles. In this article, we report the results of a survey for adaptins from sequenced genomes including those of man, mouse, the fruit fly Dr...

journal_title：Molecular biology of the cell

authors： Boehm M,Bonifacino JS

更新日期：2001-10-01 00:00:00

abstract：:The homotypic fusion of yeast vacuoles requires the Rab-family GTPase Ypt7p and its effector complex, homotypic fusion and vacuole protein sorting complex (HOPS). Although the vacuolar kinase Yck3p is required for the sensitivity of vacuole fusion to proteins that regulate the Rab GTPase cycle-Gdi1p (GDP-dissociation ...

journal_title：Molecular biology of the cell

authors： Zick M,Wickner W

更新日期：2012-09-01 00:00:00

abstract：:Survivin, a subunit of the chromosome passenger complex (CPC), binds the N-terminal tail of histone H3, which is phosphorylated on T3 by Haspin kinase, and localizes the complex to the inner centromeres. We used x-ray crystallography to determine the residues of Survivin that are important in binding phosphomodified h...

journal_title：Molecular biology of the cell

authors： Niedzialkowska E,Wang F,Porebski PJ,Minor W,Higgins JM,Stukenberg PT

更新日期：2012-04-01 00:00:00

abstract：:In mouse embryoid bodies, mutation of the tight junction protein cingulin results in changes in gene expression. Here, we studied the function of cingulin using a gene silencing approach in Madin-Darby canine kidney (MDCK) cells. Cingulin-depleted cells show higher protein and mRNA levels of claudin-2 and ZO-3, increa...

journal_title：Molecular biology of the cell

authors： Guillemot L,Citi S

更新日期：2006-08-01 00:00:00

abstract：:Attack by the host powdery mildew Erysiphe cichoracearum usually results in successful penetration and rapid proliferation of the fungus on Arabidopsis. By contrast, the nonhost barley powdery mildew Blumeria graminis f. sp. hordei (Bgh) typically fails to penetrate Arabidopsis epidermal cells. In both instances the p...

journal_title：Molecular biology of the cell

authors： Assaad FF,Qiu JL,Youngs H,Ehrhardt D,Zimmerli L,Kalde M,Wanner G,Peck SC,Edwards H,Ramonell K,Somerville CR,Thordal-Christensen H

更新日期：2004-11-01 00:00:00

abstract：:Melanosomes are tissue-specific organelles within which melanin is synthesized and stored. The melanocyte-specific glycoprotein Pmel17 is enriched in the lumen of premelanosomes, where it associates with characteristic striations of unknown composition upon which melanin is deposited. However, Pmel17 is synthesized as...

journal_title：Molecular biology of the cell

authors： Berson JF,Harper DC,Tenza D,Raposo G,Marks MS

更新日期：2001-11-01 00:00:00

abstract：:Shiga-toxin-producing Escherichia coli remain a food-borne health threat. Shiga toxin is endocytosed by intestinal epithelial cells and transported retrogradely through the secretory pathway. It is ultimately translocated to the cytosol where it inhibits protein translation. We found that Shiga toxin transport through...

journal_title：Molecular biology of the cell

authors： Hehnly H,Longhini KM,Chen JL,Stamnes M

更新日期：2009-10-01 00:00:00

abstract：:More than 97% of mice in which the C-terminal region of connexin43 (Cx43) was removed (designated as Cx43K258stop) die shortly after birth due to a defect of the epidermal barrier. The abnormal expression of Cx43K258stop protein in the uppermost layers of the epidermis seems to perturb terminal differentiation of kera...

journal_title：Molecular biology of the cell

authors： Maass K,Ghanem A,Kim JS,Saathoff M,Urschel S,Kirfel G,Grümmer R,Kretz M,Lewalter T,Tiemann K,Winterhager E,Herzog V,Willecke K

更新日期：2004-10-01 00:00:00

abstract：:During vertebrate neuromuscular junction (NMJ) assembly, motor axons and their muscle targets exchange short-range signals that regulate the subsequent steps of presynaptic and postsynaptic specialization. We report here that this interaction is in part mediated by axonal filopodia extended preferentially by cultured ...

journal_title：Molecular biology of the cell

authors： Li PP,Chen C,Lee CW,Madhavan R,Peng HB

更新日期：2011-07-15 00:00:00

abstract：:A provisional matrix consisting of fibrin and fibronectin (FN) is deposited at sites of tissue damage and repair. This matrix serves as a scaffold for fibroblast migration into the wound where these cells deposit new matrix to replace lost or damaged tissue and eventually contract the matrix to bring the margins of th...

journal_title：Molecular biology of the cell

authors： Midwood KS,Schwarzbauer JE

更新日期：2002-10-01 00:00:00

abstract：:The yeast Golgi membrane protein Rer1p is required for the retrieval of various endoplasmic reticulum (ER) membrane proteins such as Sec12p and Sec71p to the ER. We demonstrate here that the transmembrane domain (TMD) of Sec71p, a type-III membrane protein, contains an ER localization signal, which is required for phy...

journal_title：Molecular biology of the cell

authors： Sato K,Sato M,Nakano A

更新日期：2003-09-01 00:00:00

abstract：:Human neutrophils generally function adherent to an extracellular matrix. We have previously reported that upon adhesion to laminin- or fibronectin-coated, but not uncoated, plastic there is a depolymerization of actin in neutrophils. This phenomenon was not affected by inhibitors of the more well-studied components o...

journal_title：Molecular biology of the cell

authors： Wang JS,Coburn JP,Tauber AI,Zaner KS

更新日期：1997-01-01 00:00:00

abstract：:EB1 proteins are ubiquitous microtubule-associated proteins involved in microtubule search and capture, regulation of microtubule dynamics, cell polarity, and chromosome stability. We have cloned a complete cDNA of Dictyostelium EB1 (DdEB1), the largest known EB1 homolog (57 kDa). Immunofluorescence analysis and expre...

journal_title：Molecular biology of the cell

authors： Rehberg M,Gräf R

更新日期：2002-07-01 00:00:00

abstract：:Clustered protocadherins (Pcdhs) are arranged in gene clusters (α, β, and γ) with variable and constant exons. Variable exons encode cadherin and transmembrane domains and ~90 cytoplasmic residues. The 14 Pcdh-αs and 22 Pcdh-γs are spliced to constant exons, which, for Pcdh-γs, encode ~120 residues of an identical cyt...

journal_title：Molecular biology of the cell

authors： O'Leary R,Reilly JE,Hanson HH,Kang S,Lou N,Phillips GR

更新日期：2011-11-01 00:00:00

abstract：:Cadherin-based cell-cell contacts are prominent sites for phosphotyrosine signaling, being enriched in tyrosine-phosphorylated proteins and tyrosine kinases and phosphatases. The functional interplay between cadherin adhesion and tyrosine kinase signaling, however, is complex and incompletely understood. In this repor...

journal_title：Molecular biology of the cell

authors： McLachlan RW,Kraemer A,Helwani FM,Kovacs EM,Yap AS

更新日期：2007-08-01 00:00:00

abstract：:Jak tyrosine kinases have a unique domain structure containing a kinase domain (JH1) adjacent to a catalytically inactive pseudokinase domain (JH2). JH2 is crucial for inhibition of basal Jak activity, but the mechanism of this regulation has remained elusive. We show that JH2 negatively regulated Jak2 in bacterial ce...

journal_title：Molecular biology of the cell

authors： Saharinen P,Vihinen M,Silvennoinen O

更新日期：2003-04-01 00:00:00

abstract：:Keratins are cytoplasmic intermediate filament proteins preferentially expressed by epithelial tissues in a site-specific and differentiation-dependent manner. The complex network of keratin filaments in stratified epithelia is tightly regulated during squamous cell differentiation. Keratin 14 (K14) is expressed in mi...

journal_title：Molecular biology of the cell

authors： Alam H,Sehgal L,Kundu ST,Dalal SN,Vaidya MM

更新日期：2011-11-01 00:00:00

abstract：:Isu, the scaffold protein on which Fe-S clusters are built in the mitochondrial matrix, plays a central role in the biogenesis of Fe-S cluster proteins. We report that the reduction in the activity of several components of the cluster biogenesis system, including the specialized Hsp70 Ssq1, causes a 15-20-fold up-regu...

journal_title：Molecular biology of the cell

authors： Andrew AJ,Song JY,Schilke B,Craig EA

更新日期：2008-12-01 00:00:00

abstract：:ELMO2 belongs to a family of scaffold proteins involved in phagocytosis and cell motility. ELMO2 can simultaneously bind integrin-linked kinase (ILK) and RhoG, forming tripartite ERI complexes. These complexes are involved in promoting β1 integrin-dependent directional migration in undifferentiated epidermal keratinoc...

journal_title：Molecular biology of the cell

authors： Jackson BC,Ivanova IA,Dagnino L

更新日期：2015-07-15 00:00:00

abstract：:Cellular differentiation and lineage commitment are considered to be robust and irreversible processes during development. Recent work has shown that mouse and human fibroblasts can be reprogrammed to a pluripotent state with a combination of four transcription factors. We hypothesized that combinatorial expression of...

journal_title：Molecular biology of the cell

authors： Ishii R,Kami D,Toyoda M,Makino H,Gojo S,Ishii T,Umezawa A

更新日期：2012-09-01 00:00:00

abstract：:A novel gene, prom-1, was isolated in a screen for Caenorhabditis elegans mutants with increased apoptosis in the germline. prom-1 encodes an F-box protein with limited homology to the putative human tumor suppressor FBXO47. Mutations in the prom-1 locus cause a strong reduction in bivalent formation, which results in...

journal_title：Molecular biology of the cell

authors： Jantsch V,Tang L,Pasierbek P,Penkner A,Nayak S,Baudrimont A,Schedl T,Gartner A,Loidl J

更新日期：2007-12-01 00:00:00

abstract：:Hyaluronan (HA) in the endothelial glycocalyx serves as a mechanotransducer for high-shear-stress-stimulated endothelial nitric oxide synthase (eNOS) phosphorylation and nitric oxide (NO) production. Low shear stress (LSS) has been shown to contribute to endothelial inflammation and atherosclerosis by impairing the ba...

journal_title：Molecular biology of the cell

authors： Kong X,Chen L,Ye P,Wang Z,Zhang J,Ye F,Chen S

更新日期：2016-12-15 00:00:00

abstract：:Dynamic regulation of cytoskeletal contractility through phosphorylation of the nonmuscle Myosin-II regulatory light chain (MRLC) provides an essential source of tension for shaping epithelial tissues. Rho GTPase and its effector kinase ROCK have been implicated in regulating MRLC phosphorylation in vivo, but evidence...

journal_title：Molecular biology of the cell

authors： Neubueser D,Hipfner DR

更新日期：2010-08-15 00:00:00

abstract：:Compositional analyses of nucleic acids and proteins have shed light on possible origins of living cells. In this work, rigorous compositional analyses of ∼5000 plasma membrane lipid constituents of 273 species in the three life domains (archaea, eubacteria, and eukaryotes) revealed a remarkable statistical paradox, i...

journal_title：Molecular biology of the cell

authors： Bansal S,Mittal A

更新日期：2015-04-01 00:00:00

abstract：:Cadherin-catenin interactions play an important role in cadherin-mediated adhesion. Here we present strong evidence that in the cadherin-catenin complex α-catenin contributes to the binding strength of another catenin, p120, to the same complex. Specifically, we found that a β-catenin-uncoupled cadherin mutant interac...

journal_title：Molecular biology of the cell

authors： Troyanovsky RB,Klingelhöfer J,Troyanovsky SM

更新日期：2011-11-01 00:00:00