Abstract:
:Cdc10-dependent transcript 1 (Cdt1) is an essential DNA replication protein whose accumulation at the end of the cell cycle promotes the formation of pre-replicative complexes and replication in the next cell cycle. Geminin is thought to be involved in licensing replication by promoting the accumulation of Cdt1 in mitosis, because decreasing the Geminin levels prevents Cdt1 accumulation and impairs DNA replication. Geminin is known to inhibit Cdt1 function; its depletion during G2 leads to DNA rereplication and checkpoint activation. Here we show that, despite rapid Cdt1 protein turnover in G2 phase, Geminin promotes Cdt1 accumulation by increasing its RNA and protein levels in the unperturbed cell cycle. Therefore, Geminin is a master regulator of cell-cycle progression that ensures the timely onset of DNA replication and prevents its rereplication.
journal_name
Proc Natl Acad Sci U S Aauthors
Ballabeni A,Zamponi R,Moore JK,Helin K,Kirschner MWdoi
10.1073/pnas.1310677110subject
Has Abstractpub_date
2013-07-23 00:00:00pages
E2848-53issue
30eissn
0027-8424issn
1091-6490pii
1310677110journal_volume
110pub_type
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