Abstract:
INTRODUCTION:Pathogen Inactivation allows to overcome microbial contamination and growth related to storage of platelets concentrates (PC) at room temperature. The aim of our study was to evaluate the platelet storage lesion extending the storage period of pathogen inactivated platelet concentrates over 7 days using an automated cytometry assay panel. METHODS:We analyzed 43 concentrates subjected to pathogen inactivation (CPPI) at 3, 5 and 7 days evaluating: platelet count, mean platelet volume, platelets at low optical density, platelets at high density, GPIIb-IIIa glycoprotein, platelet microparticles, lactate dehydrogenase. The collection bags (Fenwal) and the IBS kit made in PL2410/PL2411 are approved for the conservation of PC up to 7 days. Data analysis was performed with anova test. RESULTS:All the parameters except small platelets and PMP were statistically different among day 7 vs. 3 and day 7 vs. 5. CONCLUSIONS:Our study showed a progressive modification of pathogen inactivated platelet concentrates observed up to 7 days. The persistence of the secretory pool and the presence of the platelet membrane fibrinogen receptor suggest the persistence of a potential hemostatic efficacy. Clinical studies are necessary to directly correlate this type of analysis to 24 h recovery or survival of transfused platelets in humans.
journal_name
Int J Lab Hematoljournal_title
International journal of laboratory hematologyauthors
Diquattro M,De Francisci G,Bonaccorso R,Tagliavia AM,Marcatti M,Palma B,Agliastro Rdoi
10.1111/ijlh.12102subject
Has Abstractpub_date
2013-12-01 00:00:00pages
637-43issue
6eissn
1751-5521issn
1751-553Xjournal_volume
35pub_type
杂志文章abstract::Successful adoptive immunotherapy for leukemia depends on the generation of T cells that can specifically react with malignant cells. Dendritic cells (DCs) are important antigen-presenting cells in the development of antileukemia T-cell responses. Mononuclear cells (MNC) were isolated from peripheral blood or bone mar...
journal_title:International journal of laboratory hematology
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journal_title:International journal of laboratory hematology
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journal_title:International journal of laboratory hematology
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journal_title:International journal of laboratory hematology
pub_type: 临床试验,杂志文章,多中心研究
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journal_title:International journal of laboratory hematology
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journal_title:International journal of laboratory hematology
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journal_title:International journal of laboratory hematology
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:International journal of laboratory hematology
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pub_type: 杂志文章,评审
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更新日期:2011-04-01 00:00:00
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journal_title:International journal of laboratory hematology
pub_type: 杂志文章
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更新日期:2017-06-01 00:00:00
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journal_title:International journal of laboratory hematology
pub_type: 杂志文章,评审
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更新日期:2018-05-01 00:00:00