Abstract：
:We used a modified pial vessel disruption (PVD) protocol with adult male Wistar rats to mimic small-vessel stroke in the cerebral cortex. Within 3 weeks, this lesion develops into a single lacuna-like cavity, which is fluid-filled and encapsulated by reactive astrocytes. Minocycline treatment that commences 1 hr after lesion and continues for 6 days prevents the cavitation and causes a filling of the lesion with reactive astrocytes and no barrier. Here, we determined whether inhibition of matrix metalloproteinases-2 and -9 (MMPs) mediates this minocycline action. Confocal microscopy revealed increased punctate staining of MMPs inside the lesion sites after 2 days of PVD. Astrocytes lined the lesion border but showed sparse localization inside the lesion. In contrast, increased MMP levels inside the lesion coincided with increased ED1 or OX-42 immunostaining, suggesting that MMP elevation reflected increased secretions from microglia/macrophages. Imaging analyses also revealed that minocycline administered for 2 days before animal euthanasia, significantly decreased MMP levels within the lesion. Moreover, Western blot analysis of cortical tissue extracts showed a significant 30-40% upregulation of MMPs 2 days after lesion. Minocycline administered 2 hr before the lesion significantly inhibited both MMP-9 and MMP-2 levels by ∼40%. In contrast, minocycline administered 1 hr after the lesion only decreased MMP-9 levels by ∼30%. Because MMP inhibition with batimastat injection also prevented cavity formation at 21 days, we conclude that minocycline prevented the creation of a lacuna-like cyst in the cerebral cortex by inhibiting the MMP secretion from microglia in the affected tissue.
journal_name
J Neurosci Resjournal_title
Journal of neuroscience researchauthors
Cayabyab FS,Gowribai K,Walz Wdoi
10.1002/jnr.23223subject
Has Abstractpub_date
2013-07-01 00:00:00pages
920-33issue
7eissn
0360-4012issn
1097-4547journal_volume
91pub_type
杂志文章abstract：:To determine whether acute hyperglycemia changes the hyperemic response to functional activation of brain, the area and magnitude of the activation were measured in healthy volunteers maintained at euglycemia and then at hyperglycemia using the hyperglycemic clamp technique. Activation of the visual cortex (8-16 Hz) w...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/1097-4547(20001015)62:2<279::AID-JNR12>3.0
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abstract：:Cortical or total brain cultures of microglia are commonly used as a model to study the inflammatory processes in Parkinson's disease. Here we characterize microglia cultures from rat ventral midbrain and evaluate their response to zymosan A. We used specific markers of microglia and evaluated the morphology, the phag...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.22219
更新日期：2010-02-15 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
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journal_title：Journal of neuroscience research
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journal_title：Journal of neuroscience research
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journal_title：Journal of neuroscience research
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490250407
更新日期：1990-04-01 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490300305
更新日期：1991-11-01 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490180112
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.24115
更新日期：2018-04-01 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.1182
更新日期：2001-09-15 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.21503
更新日期：2008-02-01 00:00:00
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doi：10.1002/jnr.10156
更新日期：2002-03-01 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490320316
更新日期：1992-07-01 00:00:00
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更新日期：2008-07-01 00:00:00
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journal_title：Journal of neuroscience research
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doi：10.1002/jnr.490400608
更新日期：1995-04-15 00:00:00
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journal_title：Journal of neuroscience research
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doi：
更新日期：1999-08-01 00:00:00
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journal_title：Journal of neuroscience research
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journal_title：Journal of neuroscience research
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abstract：:To scale up human neural stem/progenitor cell (NSPC) cultures for clinical use, we need to know how long these cells can live ex vivo without losing their ability to proliferate and differentiate; thus, a convenient method is needed to estimate the proliferative activity of human NSPCs grown in neurosphere cultures, a...
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