Abstract：
:In bacteria, archaea, fungi and plants the Trk, Ktr and HKT ion transporters are key components of osmotic regulation, pH homeostasis and resistance to drought and high salinity. These ion transporters are functionally diverse: they can function as Na(+) or K(+) channels and possibly as cation/K(+) symporters. They are closely related to potassium channels both at the level of the membrane protein and at the level of the cytosolic regulatory domains. Here we describe the crystal structure of a Ktr K(+) transporter, the KtrAB complex from Bacillus subtilis. The structure shows the dimeric membrane protein KtrB assembled with a cytosolic octameric KtrA ring bound to ATP, an activating ligand. A comparison between the structure of KtrAB-ATP and the structures of the isolated full-length KtrA protein with ATP or ADP reveals a ligand-dependent conformational change in the octameric ring, raising new ideas about the mechanism of activation in these transporters.
journal_name
Naturejournal_title
Natureauthors
Vieira-Pires RS,Szollosi A,Morais-Cabral JHdoi
10.1038/nature12055subject
Has Abstractpub_date
2013-04-18 00:00:00pages
323-8issue
7445eissn
0028-0836issn
1476-4687pii
nature12055journal_volume
496pub_type
杂志文章相关文献
NATURE文献大全abstract：:Cyclic GMP has been implicated as a messenger molecule involved in visual transduction. Photoexcited rhodopsin (R*) binds to a multisubunit membrane protein called transducin (T) and stimulates the exchange of a bound GDP molecule for GTP. This leads to the release of the alpha-subunit of T with bound GTP (T alpha-GTP...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/311659a0
更新日期：1984-10-18 00:00:00
abstract：:Although cell migration is crucial for neural development, molecular mechanisms guiding neuronal migration have remained unclear. Here we report that the secreted protein Slit repels neuronal precursors migrating from the anterior subventricular zone in the telencephalon to the olfactory bulb. Our results provide a di...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/22477
更新日期：1999-07-22 00:00:00
abstract：:Regulation of cell proliferation is necessary for immune responses, tissue repair, and upkeep of organ function to maintain human health. When proliferating cells complete mitosis, a fraction of newly born daughter cells immediately enter the next cell cycle, while the remaining cells in the same population exit to a ...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature23880
更新日期：2017-09-21 00:00:00
abstract：:Grid cells in the entorhinal cortex of freely moving rats provide a strikingly periodic representation of self-location which is indicative of very specific computational mechanisms. However, the existence of grid cells in humans and their distribution throughout the brain are unknown. Here we show that the preferred ...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature08704
更新日期：2010-02-04 00:00:00
abstract：:The immunoglobulin superfamily is a group of proteins, each made of one or several domains sharing key structural features with either the variable (V) or the constant (C) immunoglobulin domains. It includes such functionally important members as the immunoglobulins themselves, major histocompatibility complex (MHC) c...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/328267a0
更新日期：1987-07-16 00:00:00
abstract：:Io leaves a magnetic footprint on Jupiter's upper atmosphere that appears as a spot of ultraviolet emission that remains fixed underneath Io as Jupiter rotates. The specific physical mechanisms responsible for generating those emissions are not well understood, but in general the spot seems to arise because of an elec...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/415997a
更新日期：2002-02-28 00:00:00
abstract：:In the nematode Caenorhabditis elegans six hypodermal cells, the vulval precursor cells, are each competent to generate vulval cells. Normally only the three nearest precursor cells to the uterine anchor cell generate the vulva (22 nuclei), while the three others fuse with the non-specialized hypodermal syncytium (hyp...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/348169a0
更新日期：1990-11-08 00:00:00
abstract：:All forms of locomotion are repetitive motor activities that require coordinated bilateral activation of muscles. The executive elements of locomotor control are networks of spinal neurons that determine gait pattern through the sequential activation of motor-neuron pools on either side of the body axis. However, litt...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature12286
更新日期：2013-08-01 00:00:00
abstract：:COPII-coated vesicles form on the endoplasmic reticulum by the stepwise recruitment of three cytosolic components: Sar1-GTP to initiate coat formation, Sec23/24 heterodimer to select SNARE and cargo molecules, and Sec13/31 to induce coat polymerization and membrane deformation. Crystallographic analysis of the Sacchar...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature01040
更新日期：2002-09-19 00:00:00
abstract：:In legumes, root nodule organogenesis is activated in response to morphogenic lipochitin oligosaccharides that are synthesized by bacteria, commonly known as rhizobia. Successful symbiotic interaction results in the formation of highly specialized organs called root nodules, which provide a unique environment for symb...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature01207
更新日期：2002-11-28 00:00:00
abstract：:Proteomics, the large-scale analysis of proteins, will contribute greatly to our understanding of gene function in the post-genomic era. Proteomics can be divided into three main areas: (1) protein micro-characterization for large-scale identification of proteins and their post-translational modifications; (2) 'differ...
journal_title：Nature
pub_type： 杂志文章,评审
doi：10.1038/35015709
更新日期：2000-06-15 00:00:00
abstract：:Recurrent chromosomal translocations involving the mixed lineage leukaemia (MLL) gene initiate aggressive forms of leukaemia, which are often refractory to conventional therapies. Many MLL-fusion partners are members of the super elongation complex (SEC), a critical regulator of transcriptional elongation, suggesting ...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature10509
更新日期：2011-10-02 00:00:00
abstract：:The origins of religion and of complex societies represent evolutionary puzzles1-8. The 'moralizing gods' hypothesis offers a solution to both puzzles by proposing that belief in morally concerned supernatural agents culturally evolved to facilitate cooperation among strangers in large-scale societies9-13. Although pr...
journal_title：Nature
pub_type： 历史文章,杂志文章
doi：10.1038/s41586-019-1043-4
更新日期：2019-04-01 00:00:00
abstract：:Molecular-scale manipulation of electronic and ionic charge accumulation in materials is the backbone of electrochemical energy storage1-4. Layered van der Waals (vdW) crystals are a diverse family of materials into which mobile ions can electrochemically intercalate into the interlamellar gaps of the host atomic latt...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/s41586-018-0205-0
更新日期：2018-06-01 00:00:00
abstract：:The maintenance of genome stability during mitosis is coordinated by the spindle assembly checkpoint (SAC) through its effector the mitotic checkpoint complex (MCC), an inhibitor of the anaphase-promoting complex (APC/C, also known as the cyclosome)1,2. Unattached kinetochores control MCC assembly by catalysing a chan...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/s41586-018-0281-1
更新日期：2018-07-01 00:00:00
abstract：:A non-discriminatory base analogue, or universal base, would be an invaluable component of oligonucleotide probes and primers for solving the design problems that arise as a result of the degeneracy of the genetic code, or when only fragmentary peptide sequence data are available. We have designed an alternative to pr...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/369492a0
更新日期：1994-06-09 00:00:00
abstract：:Nerve growth factor (NGF) activity was not detected by bioassay in irides killed immediately after excision but NGF appeared within 24 h in living irides placed in culture or grafted to a host eye. Furthermore, sensory and, although less effective, sympathetic denervation of irides in situ led within 10 days to the ap...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/286025a0
更新日期：1980-07-03 00:00:00
abstract：:In contrast to microRNAs and Piwi-associated RNAs, short interfering RNAs (siRNAs) are seemingly dispensable for host-directed gene regulation in Drosophila. This notion is based on the fact that mutants lacking the core siRNA-generating enzyme Dicer-2 or the predominant siRNA effector Argonaute 2 are viable, fertile ...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature07015
更新日期：2008-06-05 00:00:00
abstract：:Fast radio bursts (FRBs) are millisecond-duration radio transients1,2 of unknown origin. Two possible mechanisms that could generate extremely coherent emission from FRBs invoke neutron star magnetospheres3-5 or relativistic shocks far from the central energy source6-8. Detailed polarization observations may help us t...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/s41586-020-2827-2
更新日期：2020-10-01 00:00:00
abstract：:All synaptically-based neuropsychological theories of learning postulate that there are changes resulting from neural activity which are long-lasting and confined to specific sets of synapses. In the past decade a form of synaptic strengthening known as long-term potentiation (LTP) has been found which results from hi...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/305717a0
更新日期：1983-10-20 00:00:00
abstract：:A membrane receptor protein for asialoglycoproteins induces voltage-dependent increases in ion conductance across a lipid bilayer, probably reflecting penetration of the protein into the bilayer towards an electrically positive pole. In the presence of specific ligand for the receptor, this penetration leads to a 'tra...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/288333a0
更新日期：1980-11-27 00:00:00
abstract：:Over the last twenty years classical views of how compound eyes work optically have undergone a series of overhauls. Exner's central concept of an optically inhomogeneous lens cylinder has survived, and such devices are now made commercially. He was wrong, however, about some crustacean eyes. They produce images by a ...
journal_title：Nature
pub_type： 杂志文章,评审
doi：10.1038/287681a0
更新日期：1980-10-23 00:00:00
abstract：:Coherent light sources can be used to manipulate the outcome of light-matter interactions by exploiting interference phenomena in the time and frequency domain. A powerful tool in this emerging field of 'quantum control' is the adaptive shaping of femtosecond laser pulses, resulting, for instance, in selective molecul...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/35102037
更新日期：2001-11-01 00:00:00
abstract：:A soluble enzyme system has been discovered which specifically recognizes and replicates plasmids containing the Escherichia coli chromosomal origin, oriC. Electron microscopy has shown that plasmid replication begins at or near oriC from which it progresses bidirectionally to completion. Control of initiation of a cy...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/296623a0
更新日期：1982-04-15 00:00:00
abstract：:Metformin is considered to be one of the most effective therapeutics for treating type 2 diabetes because it specifically reduces hepatic gluconeogenesis without increasing insulin secretion, inducing weight gain or posing a risk of hypoglycaemia. For over half a century, this agent has been prescribed to patients wit...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature13270
更新日期：2014-06-26 00:00:00
abstract：:A safe and effective vaccine against COVID-19 is urgently needed in quantities sufficient to immunise large populations. We report the preclinical development of two BNT162b vaccine candidates, which contain lipid-nanoparticle (LNP) formulated nucleoside-modified mRNA encoding SARS-CoV-2 spike glycoprotein-derived imm...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/s41586-021-03275-y
更新日期：2021-02-01 00:00:00
abstract：:The seemingly endless studies of the ethical connotations of genetics serve a valuable role in general understanding even if the ethical problems involved are often insubstantial. ...
journal_title：Nature
pub_type： 社论
doi：10.1038/356365a0
更新日期：1992-04-02 00:00:00
abstract：:Activation of Janus kinase 2 (JAK2) by chromosomal translocations or point mutations is a frequent event in haematological malignancies. JAK2 is a non-receptor tyrosine kinase that regulates several cellular processes by inducing cytoplasmic signalling cascades. Here we show that human JAK2 is present in the nucleus o...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature08448
更新日期：2009-10-08 00:00:00
abstract：:The interplay between magnetic fields and interacting particles can lead to exotic phases of matter that exhibit topological order and high degrees of spatial entanglement. Although these phases were discovered in a solid-state setting, recent innovations in systems of ultracold neutral atoms-uncharged atoms that do n...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature22811
更新日期：2017-06-21 00:00:00
abstract：:Over half of the inherited predisposition to insulin-dependent diabetes mellitus maps to the region of chromosome 6 that contains the highly polymorphic HLA class II genes which determine immune responsiveness. Analysis of DNA sequences from diabetics indicates that alleles of HLA-DQ beta determine both disease suscep...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/329599a0
更新日期：1987-10-15 00:00:00
