Abstract：
BACKGROUND:Wheat straw used for bioethanol production varies in enzymatic digestibility according to chemical structure and composition of cell walls and tissues. In this work, the two biologically different wheat straw organs, leaves and stems, are described together with the effects of hydrothermal pretreatment on chemical composition, tissue structure, enzyme adhesion and digestion. To highlight the importance of inherent cell wall characteristics and the diverse effects of mechanical disruption and biochemical degradation, separate leaves and stems were pretreated on lab-scale and their tissue structures maintained mostly intact for image analysis. Finally, samples were enzymatically hydrolysed to correlate digestibility to chemical composition, removal of polymers, tissue composition and disruption, particle size and enzyme adhesion as a result of pretreatment and wax removal. For comparison, industrially pretreated wheat straw from Inbicon A/S was included in all the experiments. RESULTS:Within the same range of pretreatment severities, industrial pretreatment resulted in most hemicellulose and epicuticular wax/cutin removal compared to lab-scale pretreated leaves and stems but also in most re-deposition of lignin on the surface. Tissues were furthermore degraded from tissues into individual cells while lab-scale pretreated samples were structurally almost intact. In both raw leaves and stems, endoglucanase and exoglucanase adhered most to parenchyma cells; after pretreatment, to epidermal cells in all the samples. Despite heavy tissue disruption, industrially pretreated samples were not as susceptible to enzymatic digestion as lab-scale pretreated leaves while lab-scale pretreated stems were the least digestible. CONCLUSIONS:Despite preferential enzyme adhesion to epidermal cells after hydrothermal pretreatment, our results suggest that the single most important factor determining wheat straw digestibility is the fraction of parenchyma cells rather than effective tissue disruption.
journal_name
Biotechnol Biofuelsjournal_title
Biotechnology for biofuelsauthors
Hansen MA,Hidayat BJ,Mogensen KK,Jeppesen MD,Jørgensen B,Johansen KS,Thygesen LGdoi
10.1186/1754-6834-6-54subject
Has Abstractpub_date
2013-04-16 00:00:00pages
54issue
1issn
1754-6834pii
1754-6834-6-54journal_volume
6pub_type
杂志文章abstract：: A range of lignocellulosic feedstocks (including agricultural, softwood and hardwood substrates) were pretreated with either sulfur dioxide-catalyzed steam or an ethanol organosolv procedure to try to establish a reliable assessment of the factors governing the minimum protein loading that could be used to achieve ef...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/1754-6834-4-3
更新日期：2011-02-10 00:00:00
abstract：Background:With the further development of anaerobic digestion, an increasing output of anaerobically digested wastewater (ADW), which typically contained high concentrations of ammonium, phosphate, and suspended solids, was inevitable. Microalgae cultivation offered a potential waste-to-value strategy to reduce the hi...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-018-1190-0
更新日期：2018-07-09 00:00:00
abstract：Background:Microalgae are attracting much attention as a promising feedstock for renewable energy production, while simultaneously providing environmental benefits. So far, comparison studies for microalgae selection for this purpose were mainly based on data obtained from batch cultures, where the lipid content and th...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-019-1461-4
更新日期：2019-05-11 00:00:00
abstract：Background:Photosynthetic oleaginous microalgae are promising feedstocks for biofuels. Acyl-CoA:diacylglycerol acyltransferases (DGATs) represent rich sources for engineering microalgal lipid production. The principal activity of DGATs has been defined as a single-function enzyme catalyzing the esterification of diacyl...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-018-1029-8
更新日期：2018-02-09 00:00:00
abstract：Background:Xylan is a major hemicellulosic component in the cell walls of higher plants especially in the secondary walls of vascular cells which are playing important roles in physiological processes and overall mechanical strength. Being the second most abundant cell wall polymer after cellulose, xylan is an abundant...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-017-0935-5
更新日期：2017-11-30 00:00:00
abstract：BACKGROUND:Microalgae accumulate a considerable amount of lipids and carbohydrate under nutrient-deficient conditions, which makes them one of the promising sustainable resources for biofuel production. In the present study, to obtain the biomass with higher lipid and carbohydrate contents, we implemented a short-term ...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-017-0747-7
更新日期：2017-03-09 00:00:00
abstract：BACKGROUND:The microbial community in anaerobic digestion is mainly monitored by means of DNA-based methods. This may lead to incorrect interpretation of the community parameters, because microbial abundance does not necessarily reflect activity. In this research, the difference between microbial community response on ...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-016-0652-5
更新日期：2016-11-09 00:00:00
abstract：BACKGROUND:In addition to efficient pentose utilization, high inhibitor tolerance is a key trait required in any organism used for economically viable industrial bioethanol production with lignocellulose biomass. Although recent work has succeeded in establishing efficient xylose fermentation in robust industrial Sacch...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/1754-6834-6-120
更新日期：2013-08-26 00:00:00
abstract：BACKGROUND:Replacing the energy-intensive evaporation of stillage by anaerobic digestion is one way of decreasing the energy demand of the lignocellulosic biomass to the ethanol process. The biogas can be upgraded and sold as transportation fuel, injected directly into the gas grid or be incinerated on-site for combine...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/1754-6834-3-21
更新日期：2010-09-15 00:00:00
abstract：Background:Cyanobacteria can be metabolically engineered to convert CO2 to fuels and chemicals such as ethylene. A major challenge in such efforts is to optimize carbon fixation and partition towards target molecules. Results:The efe gene encoding an ethylene-forming enzyme was introduced into a strain of the cyanobac...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-020-1653-y
更新日期：2020-01-28 00:00:00
abstract：BACKGROUND:Multidimensional solid-state nuclear magnetic resonance (ssNMR) spectroscopy has emerged as an indispensable technique for resolving polymer structure and intermolecular packing in primary and secondary plant cell walls. Isotope (13C) enrichment provides feasible sensitivity for measuring 2D/3D correlation s...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-020-01858-x
更新日期：2021-01-07 00:00:00
abstract：BACKGROUND:Microbial lipid production using renewable feedstock shows great promise for the biodiesel industry. RESULTS:In this study, the ability of a lipid-engineered Yarrowia lipolytica strain JMY4086 to produce lipids using molasses and crude glycerol under different oxygenation conditions and at different inoculu...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-015-0286-z
更新日期：2015-07-25 00:00:00
abstract：BACKGROUND:Engineering of the yeast Saccharomyces cerevisiae for improved utilization of pentose sugars is vital for cost-efficient cellulosic bioethanol production. Although endogenous hexose transporters (Hxt) can be engineered into specific pentose transporters, they remain subjected to glucose-regulated protein deg...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-016-0573-3
更新日期：2016-07-26 00:00:00
abstract：BACKGROUND:The productivity of an algal culture depends on how efficiently it converts sunlight into biomass and lipids. Wild-type algae in their natural environment evolved to compete for light energy and maximize individual cell growth; however, in a photobioreactor, global productivity should be maximized. Improving...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-015-0337-5
更新日期：2015-09-25 00:00:00
abstract：BACKGROUND:D-2,3-butanediol has many industrial applications such as chiral reagents, solvents, anti-freeze agents, and low freezing point fuels. Traditional D-2,3-butanediol producing microorganisms, such as Klebsiella pneumonia and K. xoytoca, are pathogenic and not capable of producing D-2,3-butanediol at high optic...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/1754-6834-7-16
更新日期：2014-01-29 00:00:00
abstract：Background:Microalgae, due to its well-recognized advantages have gained renewed interest as potentially good feedstock for biodiesel. Production of fatty acid methyl esters (FAMEs) as a type of biodiesel was carried out from Chlorella vulgaris bio-oil. Biodiesel was produced in the presence of nano-biocatalysts compos...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-020-01688-x
更新日期：2020-03-20 00:00:00
abstract：BACKGROUND:The model bacterium Clostridium cellulolyticum efficiently degrades crystalline cellulose and hemicellulose, using cellulosomes to degrade lignocellulosic biomass. Although it imports and ferments both pentose and hexose sugars to produce a mixture of ethanol, acetate, lactate, H2 and CO2, the proportion of ...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/1754-6834-5-2
更新日期：2012-01-04 00:00:00
abstract：BACKGROUND:Bamboo is potentially an interesting feedstock for advanced bioethanol production in China due to its natural abundance, rapid growth, perennial nature and low management requirements. Liquid hot water (LHW) pretreatment was selected as a promising technology to enhance sugar release from bamboo lignocellulo...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/1754-6834-6-173
更新日期：2013-11-29 00:00:00
abstract：BACKGROUND:Low cost of raw materials and good process yields are necessary for future lignocellulosic biomass biorefineries to be sustainable and profitable. A low cost feedstock will be diverse, changing as a function of seasonality and price and will most likely be available from multiple sources to the biorefinery. ...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-015-0414-9
更新日期：2015-12-24 00:00:00
abstract：Background:For bioethanol production from lignocellulosic biomass, phenolics derived from pretreatment have been generally considered as highly inhibitory towards enzymatic hydrolysis and fermentation. As phenolics are produced from lignin degradation during pretreatment, it is likely that the pretreatment will exert a...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-020-01686-z
更新日期：2020-03-11 00:00:00
abstract：Background:2,3-Butanediol (2,3-BDO) is a valuable chemical for industrial applications. Bacteria can produce 2,3-BDO with a high productivity, though most of their classification as pathogens makes them undesirable for the industrial-scale production. Though Saccharomyces cerevisiae (GRAS microorganism) was engineered ...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-019-1545-1
更新日期：2019-08-29 00:00:00
abstract：BACKGROUND:Inefficient carbohydrate conversion has been an unsolved problem for various lignocellulosic biomass pretreatment technologies, including AFEX, dilute acid, and ionic liquid pretreatments. Previous work has shown 22% of total carbohydrates are typically unconverted, remaining as soluble or insoluble oligomer...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-017-0757-5
更新日期：2017-03-29 00:00:00
abstract：:[This corrects the article DOI: 10.1186/s13068-018-1097-9.]. ...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章,已发布勘误
doi：10.1186/s13068-018-1185-x
更新日期：2018-07-03 00:00:00
abstract：:Lignin is the most abundant aromatic substrate on Earth and its valorization technologies are still under developed. Depolymerization and fragmentation are the predominant preparatory strategies for valorization of lignin to chemicals and fuels. However, due to the structural heterogeneity of lignin, depolymerization ...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章,评审
doi：10.1186/s13068-019-1376-0
更新日期：2019-02-15 00:00:00
abstract：BACKGROUND:Alkaline hydrogen peroxide pretreatment catalyzed by Cu(II) 2,2'-bipyridine complexes has previously been determined to substantially improve the enzymatic hydrolysis of woody plants including hybrid poplar as a consequence of moderate delignification. In the present work, cell wall morphological and lignin ...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-015-0300-5
更新日期：2015-08-20 00:00:00
abstract：Background:Part-stream low-frequency ultrasound (LFUS) was one of the common practices for sludge disintegration in full-scale anaerobic digestion (AD) facilities. However, the effectiveness of part-stream LFUS treatment and its effect on AD microbiome have not been fully elucidated. Methods:Here we testified the effe...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-018-1042-y
更新日期：2018-02-21 00:00:00
abstract：BACKGROUND:Recently developed iron cocatalyst enhancement of dilute acid pretreatment of biomass is a promising approach for enhancing sugar release from recalcitrant lignocellulosic biomass. However, very little is known about the underlying mechanisms of this enhancement. In the current study, our aim was to identify...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/1754-6834-4-48
更新日期：2011-11-10 00:00:00
abstract：BACKGROUND:The most advanced strains of xylose-fermenting Saccharomyces cerevisiae still utilize xylose far less efficiently than glucose, despite the extensive metabolic and evolutionary engineering applied in their development. Systematic comparison of strains across literature is difficult due to widely varying cond...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-017-0887-9
更新日期：2017-09-04 00:00:00
abstract：UNLABELLED: BACKGROUND:In the present study, three ionic liquids, namely 1-butyl-3-methylimidazolium chloride ([BMIM]Cl), 1-ethyl-3-methylimidazolium acetate ([EMIM]OAc), and 1-ethyl-3-methylimidazolium diethyl phosphate ([EMIM]DEP), were used to partially dissolve rice husk, after which the cellulose were regenerated...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/1754-6834-5-67
更新日期：2012-09-07 00:00:00
abstract：Background:Understanding the extracellular electron transport pathways in cyanobacteria is a major factor towards developing biophotovoltaics. Stressing cyanobacteria cells environmentally and then probing changes in physiology or metabolism following a significant change in electron transfer rates is a common approach...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-020-01788-8
更新日期：2020-08-26 00:00:00
