Imaging burst kinetics and spatial coordination during serial killing by single natural killer cells.

abstract：:Highly multiplexed assays using antibody coated, fluorescent (xMap) beads are widely used to measure quantities of soluble analytes, such as cytokines and antibodies in clinical and other studies. Current analyses of these assays use methods based on standard curves that have limitations in detecting low or high abund...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Won JH,Goldberger O,Shen-Orr SS,Davis MM,Olshen RA

更新日期：2012-02-21 00:00:00

abstract：:The present paper summarizes future needs in information and tools, technology, infrastructure, training, funding, and bioinformatics, to provide the genomic knowledge and tools for breeding and biotechnological goals in maize. The National Corn Genome Initiative (NCGA) has developed through actions taken by the Natio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Coe EH

更新日期：1998-03-03 00:00:00

abstract：:The complex prokaryote, Myxococcus xanthus, undergoes a program of multicellular development when starved for nutrients, culminating in sporulation. M. xanthus makes MglA, a 22-kDa, soluble protein that is required for both multicellular development and gliding motility. MglA is similar in sequence to the Saccharomyce...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hartzell PL

更新日期：1997-09-02 00:00:00

abstract：:Proper regulation of the intracellular ion concentration is essential to maintain life and is achieved by ion transporters that transport their substrates across the membrane in a strictly regulated manner. MgtE is a Mg(2+) transporter that may function in the homeostasis of the intracellular Mg(2+) concentration. A r...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ishitani R,Sugita Y,Dohmae N,Furuya N,Hattori M,Nureki O

更新日期：2008-10-07 00:00:00

abstract：:Suppressor T cells have been shown to be much more radiosensitive than other lymphoïd cells, and we have tried to reduce tumor growth by low-dose irradiation. Syngeneic DBA/2 mice received whole-body irradiation (150 rads; 1 rad = 0.01 J/kg) 6 days after P815 tumor inoculation. Tumor growth is significantly reduced in...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tilkin AF,Schaaf-Lafontaine N,Van Acker A,Boccadoro M,Urbain J

更新日期：1981-03-01 00:00:00

abstract：:We report here the cloning, expression, and characterization of a dual-substrate, cAMP and cGMP, cyclic nucleotide phosphodiesterase (PDE) from mouse. This PDE contains the consensus sequence for a PDE catalytic domain, but shares <50% sequence identity with the catalytic domains of all other known PDEs and, therefore...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Soderling SH,Bayuga SJ,Beavo JA

更新日期：1999-06-08 00:00:00

abstract：:Outdoor ozone transported indoors initiates oxidative chemistry, forming volatile organic products. The influence of ozone chemistry on indoor air composition has not been directly quantified in normally occupied residences. Here, we explore indoor ozone chemistry in a house in California with two adult inhabitants. W...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu Y,Misztal PK,Arata C,Weschler CJ,Nazaroff WW,Goldstein AH

更新日期：2021-02-09 00:00:00

abstract：:Pore-forming toxins (PFTs) are a class of proteins implicated in a wide range of virulent bacterial infections and diseases. These toxins bind to target membranes and subsequently oligomerize to form functional pores that eventually lead to cell lysis. While the protein undergoes large conformational changes on the bi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ponmalar II,Cheerla R,Ayappa KG,Basu JK

更新日期：2019-06-25 00:00:00

abstract：:Most marine organisms disperse via ocean currents as larvae, so it is often assumed that larval-stage duration is the primary determinant of geographic range size. However, empirical tests of this relationship have yielded mixed results, and alternative hypotheses have rarely been considered. Here we assess the relati...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Luiz OJ,Allen AP,Robertson DR,Floeter SR,Kulbicki M,Vigliola L,Becheler R,Madin JS

更新日期：2013-10-08 00:00:00

abstract：:The nonclassical MHC class I-related (MHC-I) molecule HFE controls cellular iron homeostasis by a mechanism that has not been fully elucidated. We examined the regulation of HFE by K5, the E3 ubiquitin ligase encoded by Kaposi's sarcoma-associated herpesvirus (KSHV/HHV8), that is known to down-regulate classical MHC-I...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rhodes DA,Boyle LH,Boname JM,Lehner PJ,Trowsdale J

更新日期：2010-09-14 00:00:00

abstract：:FRET between the zinc porphyrin (ZnP) chromophore in zinc-substituted cytochrome c (Zn-cyt c) and an Alexa Fluor dye attached to specific surface sites was used to characterize Zn-cyt c unfolding. The use of ZnP as a fluorescent acceptor eliminates the need to doubly label the protein with exogenous dyes to perform FR...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ensign AA,Jo I,Yildirim I,Krauss TD,Bren KL

更新日期：2008-08-05 00:00:00

abstract：:Primary prostate cancer is the most common malignancy in men but has highly variable outcomes, highlighting the need for biomarkers to determine which patients can be managed conservatively. Few large prostate oncogenome resources currently exist that combine the molecular and clinical outcome data necessary to discov...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hieronymus H,Schultz N,Gopalan A,Carver BS,Chang MT,Xiao Y,Heguy A,Huberman K,Bernstein M,Assel M,Murali R,Vickers A,Scardino PT,Sander C,Reuter V,Taylor BS,Sawyers CL

更新日期：2014-07-29 00:00:00

abstract：:Model organism studies have demonstrated that cell fate specification decisions play an important role in normal brain development. Their role in human neurodevelopmental disorders, however, is poorly understood, with very few examples described. The cerebellum is an excellent system to study mechanisms of cell fate s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Millen KJ,Steshina EY,Iskusnykh IY,Chizhikov VV

更新日期：2014-04-29 00:00:00

abstract：:Photoperiodic control of testis growth in Passer domesticus (house sparrow) is mediated entirely by extraretinal photoreceptors in the brain. The eyes do not participate in photoperiodically significant photoreception. Removal of the pineal organ does not affect either the response to light or, to a first approximatio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Menaker M,Roberts R,Elliott J,Underwood H

更新日期：1970-09-01 00:00:00

abstract：:Among the tRNA population of the archaeal parasite Nanoarchaeum equitans are five species assembled from separate 5' and 3' tRNA halves and four species derived from tRNA precursors containing introns. In both groups an intervening sequence element must be removed during tRNA maturation. A bulge-helix-bulge (BHB) moti...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Randau L,Calvin K,Hall M,Yuan J,Podar M,Li H,Söll D

更新日期：2005-12-13 00:00:00

abstract：:In many plants, osmotic stress induces a rapid accumulation of proline through de novo synthesis from glutamate. This response is thought to play a pivotal role in osmotic stress tolerance [Kishor, P. B. K., Hong, Z., Miao, G.-H., Hu, C.-A. A. and Verma, D. P. S. (1995) Plant Physiol. 108, 1387-1394]. During recovery ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Verbruggen N,Hua XJ,May M,Van Montagu M

更新日期：1996-08-06 00:00:00

abstract：:Adrenocortical carcinomas (ACCs) are rare and highly malignant cancers associated with poor survival of patients. Currently, mitotane, a nonspecific derivative of the pesticide DDT (1,1-(dichlorobiphenyl)-2,2-dichloroethane), is used as the standard treatment, but its mechanism of action in ACCs remains elusive. Here ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Belavgeni A,Bornstein SR,von Mässenhausen A,Tonnus W,Stumpf J,Meyer C,Othmar E,Latk M,Kanczkowski W,Kroiss M,Hantel C,Hugo C,Fassnacht M,Ziegler CG,Schally AV,Krone NP,Linkermann A

更新日期：2019-10-29 00:00:00

abstract：:The protooncogene c-fps/fes is a single vertebrate locus homologous to both the fps transforming gene of Fujinami avian sarcoma virus and the fes transforming gene of two feline sarcoma virus isolates. The human c-fps/fes locus has been previously cloned and characterized. We report that a recombinant gene in which 3'...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sodroski JG,Goh WC,Haseltine WA

更新日期：1984-05-01 00:00:00

abstract：:We have isolated and determined the sequence of a genomic clone containing the gene for a human class I transplantation antigen. The gene contains seven exons. The first five exons code respectively for a signal peptide, for the first, second, and third extracellular domains of the protein molecule, and for the transm...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Malissen M,Malissen B,Jordan BR

更新日期：1982-02-01 00:00:00

abstract：:Excessive activation of calmodulin kinase II (CaMKII) causes arrhythmias and heart failure, but the cellular mechanisms for CaMKII-targeted proteins causing disordered cell membrane excitability and myocardial dysfunction remain uncertain. Failing human cardiomyocytes exhibit increased CaMKII and voltage-gated Ca(2+) ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Koval OM,Guan X,Wu Y,Joiner ML,Gao Z,Chen B,Grumbach IM,Luczak ED,Colbran RJ,Song LS,Hund TJ,Mohler PJ,Anderson ME

更新日期：2010-03-16 00:00:00

abstract：:The importance of gene gain through duplication has long been appreciated. In contrast, the importance of gene loss has only recently attracted attention. Indeed, studies in organisms ranging from plants to worms and humans suggest that duplication of some genes might be better tolerated than that of others. Here we h...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： De Smet R,Adams KL,Vandepoele K,Van Montagu MC,Maere S,Van de Peer Y

更新日期：2013-02-19 00:00:00

abstract：:The envelope spike of HIV is one of the most highly N-glycosylated structures found in nature. However, despite extensive research revealing essential functional roles in infection and immune evasion, the chemical structures of the glycans on the native viral envelope glycoprotein gp120--as opposed to recombinantly ge...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Doores KJ,Bonomelli C,Harvey DJ,Vasiljevic S,Dwek RA,Burton DR,Crispin M,Scanlan CN

更新日期：2010-08-03 00:00:00

abstract：:We report an approach for studying protein-induced DNA bends in solution that is based on measuring the sizes of circular DNA molecules by using two-dimensional gel electrophoresis. These circular fragments are obtained by ligating short synthetic oligonucleotides containing a protein-recognition region in the presenc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lyubchenko Y,Shlyakhtenko L,Chernov B,Harrington RE

更新日期：1991-06-15 00:00:00

abstract：:This paper shows that the most predictable components of internal variability in coupled atmosphere-ocean models are remarkably similar to the most predictable components of climate models without interactive ocean dynamics (i.e., models whose ocean is represented by a 50-m-deep slab ocean mixed layer with no interact...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Srivastava A,DelSole T

更新日期：2017-02-28 00:00:00

abstract：:The efficacy of active immunization with the cocaine immunogen GNC-keyhole limpet hemocyanin (KLH) in preventing cocaine self-administration reinstatement was assessed in rats. An animal model of relapse was used where rats were trained to self-administer cocaine, subjected to a period of extinction by substituting th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Carrera MR,Ashley JA,Zhou B,Wirsching P,Koob GF,Janda KD

更新日期：2000-05-23 00:00:00

abstract：:Combinatorial antibody libraries have the potential to display the entire immunological record of an individual, allowing one to detect and recover any antibody ever made, irrespective of whether it is currently being produced. We have termed this the "fossil record" of an individual's antibody response. To determine ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Felding-Habermann B,Lerner RA,Lillo A,Zhuang S,Weber MR,Arrues S,Gao C,Mao S,Saven A,Janda KD

更新日期：2004-12-07 00:00:00

abstract：:We used a high-titer recombinant adeno-associated virus (rAAV) vector to express WT or mutant human alpha-synuclein in the substantia nigra of adult marmosets. The alpha-synuclein protein was expressed in 90-95% of all nigral dopamine neurons and distributed by anterograde transport throughout their axonal and dendrit...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kirik D,Annett LE,Burger C,Muzyczka N,Mandel RJ,Björklund A

更新日期：2003-03-04 00:00:00

abstract：:The Mauer mandible, holotype of Homo heidelbergensis, was found in 1907 in fluvial sands deposited by the Neckar River 10 km southeast of Heidelberg, Germany. The fossil is an important key to understanding early human occupation of Europe north of the Alps. Given the associated mammal fauna and the geological context...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wagner GA,Krbetschek M,Degering D,Bahain JJ,Shao Q,Falguères C,Voinchet P,Dolo JM,Garcia T,Rightmire GP

更新日期：2010-11-16 00:00:00

abstract：:A mutant of E. coli defective in DNA polymerase II activity was isolated. Extracts had about 0.1% of the normal activity of the DNA polymerase. The effect of the mutation on the ability of cells to replicate DNA of various sources was analyzed. Mutant bacteria grow normally, at 41 degrees as well as at 30 degrees . Al...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hirota Y,Gefter M,Mindich L

更新日期：1972-11-01 00:00:00

abstract：:Replication of plasmid DNA molecules containing the simian virus 40 (SV40) origin of DNA replication has been reconstituted with seven highly purified cellular proteins plus the SV40 large tumor (T) antigen. Initiation of DNA synthesis is absolutely dependent upon T antigen, replication protein A, and the DNA polymera...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Weinberg DH,Collins KL,Simancek P,Russo A,Wold MS,Virshup DM,Kelly TJ

更新日期：1990-11-01 00:00:00