Abstract：
:A critical study of the use of the Fe(III)/3-hydroxy-4-nitroso-2,7-naphthalenedisulfonic acid (NRS) complexes on the quantification of the polyphenols content in aqueous extracts of plants, expressed as pyrogallic acid, [PA], is presented. The reaction used is based on the reduction of Fe(III) to Fe(II) by [PA] in the presence of NRS in a buffered medium (Tris; pH 8.0) with formation of Fe(II)/NRS complexes. A calibration curve of absorbance (at 730 nm) vs. [PA] is linear (r=0.998; n=7) from 1.0 to 7.0 μmolL(-1) [PA]. LD and RSD were 0.5 μmolL(-1) and 2.5% (6.0 μmolL(-1) [PA], n=10), respectively. The influence of pH, type of buffer solution and interfering species possibly present in the samples were evaluated. Aqueous extracts of twelve medicinal plants used in the Brazilian folk medicine were analysed by using both Fe(III)/NRS complexes and the Folin-Ciocalteu reagent.
journal_name
Food Chemjournal_title
Food chemistryauthors
da Silva DX,de Souza MW,Corrêa Cdos S,Moya HDdoi
10.1016/j.foodchem.2012.11.045subject
Has Abstractpub_date
2013-06-01 00:00:00pages
1325-32issue
2-3eissn
0308-8146issn
1873-7072pii
S0308-8146(12)01776-1journal_volume
138pub_type
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