Abstract：
:Direct competitive enzyme-linked immunosorbent assay (ELISA) for 2,4-dichlorophenoxyacetic acid (2,4-D) was developed. Varying the concentrations of monoclonal anti-2,4-D-antibody and the conjugate of soybean peroxidase and 2,4-D the conditions of ELISA performance were optimised. The chemiluminescent method based on peroxidase-catalysed oxidation of luminol was applied to measure the enzyme activity of the conjugate. A mixture of 3-(10'-phenothiazinyl)propane-1-sulfonate and 4-morpholinopyridine was used as potent enhancer of chemiluminescence signal. It was shown that the values of the lower detection limit, IC50 and the working range were 1.5, 64.0, and 6.5-545ng/mL, respectively. The recovery values of CL-ELISA from 10 spiked samples of oranges (n=5) and mandarins (n=5) cultivated in green house without use of 2,4-D and containing different 2,4-D concentrations (10-300ng/mL) were ranged from 92% to 104% that indicated on the absence of matrix effect for the fruit extracts of interest. Determination of 2,4-D in peel of five oranges and five mandarins purchased from stores in Vietnam showed that 2,4-D content in oranges fruits (79-104μg/kg) was significantly higher than that in mandarins (1.66-2.82μg/kg).
journal_name
Food Chemjournal_title
Food chemistryauthors
Vdovenko MM,Stepanova AS,Eremin SA,Van Cuong N,Uskova NA,Yu Sakharov Idoi
10.1016/j.foodchem.2013.04.060subject
Has Abstractpub_date
2013-11-15 00:00:00pages
865-8issue
2eissn
0308-8146issn
1873-7072pii
S0308-8146(13)00506-2journal_volume
141pub_type
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