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Optimization of the preparation of fish protein anti-obesity hydrolysates using response surface methodology.

Abstract：

:The enzymatic condition for producing the anti-obesity hydrolysates from fish water-soluble protein was optimized with the aid of response surface methodology, which also derived a statistical model for experimental validation. Compared with neutral protease, papain and protamex, the porcine pancreas lipase inhibitory rate of hydrolysates from fish water-soluble protein was higher with alkaline protease. Results showed that the model terms were significant, the terms of lack of fit were not significant, and the optimal conditions for the hydrolysis by alkaline protease were initial pH 11, temperature 39 °C, enzyme dosage 122 U/mL and 10 h of hydrolysis time. Under these conditions, the porcine pancreas lipase and the α-amylase inhibitory rate could reach 53.04% ± 1.32% and 20.03 ± 0.89%, while predicted value were 54.63% ± 1.75%, 21.22% ± 0.70%, respectively. In addition, Lineweaver-Burk plots showed noncompetitive inhibition. The K(i) value calculated was 84.13 mg/mL. These results demonstrated that fish water-soluble protein could be used for obtaining anti-obesity hydrolysates.

journal_name

Int J Mol Sci

journal_title

International journal of molecular sciences

authors

Liu L,Wang Y,Peng C,Wang J

doi

10.3390/ijms14023124

subject

Has Abstract

pub_date

2013-02-01 00:00:00

pages

3124-39

issue

2

issn

1422-0067

pii

ijms14023124

journal_volume

14

pub_type

杂志文章

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