Effect of lidocaine on dynamic changes in cortical reduced nicotinamide adenine dinucleotide fluorescence during transient focal cerebral ischemia in rats.


:Rats were subjected to 90min of focal ischemia by occluding the left middle cerebral and both common carotid arteries. The dynamic changes in the formation of brain ischemic areas were analyzed by measuring the direct current (DC) potential and reduced nicotinamide adenine dinucleotide (NADH) fluorescence with ultraviolet irradiation. In the lidocaine group (n=10), 30min before ischemia, an intravenous bolus (1.5mg/kg) of lidocaine was administered, followed by a continuous infusion (2mg/kg/h) for 150min. In the control group (n=10), an equivalent amount of saline was administered. Following the initiation of ischemia, an area of high-intensity NADH fluorescence rapidly developed in the middle cerebral artery territory in both groups and the DC potential in this area showed ischemic depolarization. An increase in NADH fluorescence closely correlated with the DC depolarization. The blood flow in the marginal zone of both groups showed a similar decrease. Five minutes after the onset of ischemia, the area of high-intensity NADH fluorescence was significantly smaller in the lidocaine group (67% of the control; P=0.01). This was likely due to the suppression of ischemic depolarization by blockage of voltage-dependent sodium channels with lidocaine. Although lidocaine administration did not attenuate the number of peri-infarct depolarizations during ischemia, the high-intensity area and infarct volume were significantly smaller in the lidocaine group both at the end of ischemia (78% of the control; P=0.046) and 24h later (P=0.02). A logistic regression analysis demonstrated a relationship between the duration of ischemic depolarization and histologic damage and revealed that lidocaine administration did not attenuate neuronal damage when the duration of depolarization was identical. These findings indicate that the mechanism by which lidocaine decreases infarct volume is primarily through a reduction of the brain area undergoing NADH fluorescence increases which closely correlates with depolarization.






Naito H,Takeda Y,Danura T,Kass IS,Morita K




Has Abstract


2013-04-03 00:00:00












  • Abnormalities in the cerebellum and brainstem in homozygous lurcher mice.

    abstract::The lurcher mutation induces Purkinje cell degeneration in heterozygous mice, and neonatal death in homozygous animals. Using the D6Mit16 Simple Sequence Length Polymorphic marker in F2 hybrids between AKR +/+ mice and B6+/Lc mice, homozygous lurcher fetuses and newborns as well as heterozygous and normal littermates ...


    pub_type: 杂志文章


    authors: Resibois A,Cuvelier L,Goffinet AM

    更新日期:1997-09-01 00:00:00

  • Oxytocin and vasopressin enhance synaptic transmission in the hypoglossal motor nucleus of young rats by acting on distinct receptor types.

    abstract::Hypoglossal (XII) motoneurons innervate extrinsic and intrinsic muscles of the tongue and control behaviors such as suckling, swallowing, breathing or chewing. In young rats, XII motoneurons express V1a vasopressin and oxytocin receptors. Previous studies have shown that activation of these receptors induces direct po...


    pub_type: 杂志文章


    authors: Wrobel LJ,Reymond-Marron I,Dupré A,Raggenbass M

    更新日期:2010-02-03 00:00:00

  • Cellular localization and differential distribution of GABAA receptor subunit proteins and messenger RNAs within hypothalamic magnocellular neurons.

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    pub_type: 杂志文章


    authors: Fenelon VS,Sieghart W,Herbison AE

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    authors: Lane DA,Patel PA,Morgan MM

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    pub_type: 杂志文章


    authors: Sanchez-Toscano F,Caminero AA,Machin C,Abella G

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  • Brainstem and hypothalamic areas activated by tissue hypoxia: Fos-like immunoreactivity induced by carbon monoxide inhalation in the rat.

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    pub_type: 杂志文章


    authors: Bodineau L,Larnicol N

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    pub_type: 杂志文章


    authors: Tonini M,Costa M,Brookes SJ,Humphreys CM

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  • Oral Dyskinesias and striatal lesions in rats after long-term co-treatment with haloperidol and 3-nitropropionic acid.

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    pub_type: 杂志文章


    authors: Andreassen OA,Ferrante RJ,Beal MF,Jørgensen HA

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  • Pharmacologic rescue of motor and sensory function by the neuroprotective compound P7C3 following neonatal nerve injury.

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    pub_type: 杂志文章


    authors: Kemp SWP,Szynkaruk M,Stanoulis KN,Wood MD,Liu EH,Willand MP,Morlock L,Naidoo J,Williams NS,Ready JM,Mangano TJ,Beggs S,Salter MW,Gordon T,Pieper AA,Borschel GH

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  • Ectopic sympathetic preganglionic neurons maintain proper connectivity in the reeler mutant mouse.

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    pub_type: 杂志文章


    authors: Yip YP,Rinaman L,Capriotti C,Yip JW

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    pub_type: 杂志文章


    authors: Cao Y,Zhang H,Lu X,Wang J,Zhang X,Sun S,Bao Z,Tian W,Ning S,Wang L,Cui L

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    pub_type: 杂志文章


    authors: Wang Z,Lin Y,Liu W,Kuang P,Lao W,Ji Y,Zhu H

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    pub_type: 杂志文章


    authors: Zhang F,Clarke JD,Santos-Ruiz L,Ferretti P

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    pub_type: 杂志文章


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    pub_type: 杂志文章


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    pub_type: 杂志文章


    authors: Zhou M,Ma T,Tseng MT

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    pub_type: 杂志文章,评审


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    pub_type: 杂志文章,评审


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    pub_type: 杂志文章


    authors: Nichols JA,Jakkamsetti VP,Salgado H,Dinh L,Kilgard MP,Atzori M

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    pub_type: 杂志文章


    authors: Sweetnam PM,Wrathall JR,Neale JH

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    pub_type: 杂志文章


    authors: Ciriello J,Caverson MM,Li Z

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    pub_type: 杂志文章


    authors: Obeso A,Rocher A,Fidone S,Gonzalez C

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    pub_type: 杂志文章


    authors: Roebuck H,Bourke P,Guo K

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    pub_type: 杂志文章


    authors: Grimmelikhuijzen CJ,Dierickx K,Boer GJ

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    pub_type: 杂志文章


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    pub_type: 杂志文章


    authors: Miao FJ,Green PG,Benowitz N,Levine JD

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    pub_type: 杂志文章


    authors: Beery AK,Zucker I

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    pub_type: 杂志文章


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    更新日期:2017-04-07 00:00:00

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    pub_type: 杂志文章


    authors: Bégou M,Volle J,Bertrand JB,Brun P,Job D,Schweitzer A,Saoud M,D'Amato T,Andrieux A,Suaud-Chagny MF

    更新日期:2008-11-11 00:00:00