Abstract:
:Rats were subjected to 90min of focal ischemia by occluding the left middle cerebral and both common carotid arteries. The dynamic changes in the formation of brain ischemic areas were analyzed by measuring the direct current (DC) potential and reduced nicotinamide adenine dinucleotide (NADH) fluorescence with ultraviolet irradiation. In the lidocaine group (n=10), 30min before ischemia, an intravenous bolus (1.5mg/kg) of lidocaine was administered, followed by a continuous infusion (2mg/kg/h) for 150min. In the control group (n=10), an equivalent amount of saline was administered. Following the initiation of ischemia, an area of high-intensity NADH fluorescence rapidly developed in the middle cerebral artery territory in both groups and the DC potential in this area showed ischemic depolarization. An increase in NADH fluorescence closely correlated with the DC depolarization. The blood flow in the marginal zone of both groups showed a similar decrease. Five minutes after the onset of ischemia, the area of high-intensity NADH fluorescence was significantly smaller in the lidocaine group (67% of the control; P=0.01). This was likely due to the suppression of ischemic depolarization by blockage of voltage-dependent sodium channels with lidocaine. Although lidocaine administration did not attenuate the number of peri-infarct depolarizations during ischemia, the high-intensity area and infarct volume were significantly smaller in the lidocaine group both at the end of ischemia (78% of the control; P=0.046) and 24h later (P=0.02). A logistic regression analysis demonstrated a relationship between the duration of ischemic depolarization and histologic damage and revealed that lidocaine administration did not attenuate neuronal damage when the duration of depolarization was identical. These findings indicate that the mechanism by which lidocaine decreases infarct volume is primarily through a reduction of the brain area undergoing NADH fluorescence increases which closely correlates with depolarization.
journal_name
Neurosciencejournal_title
Neuroscienceauthors
Naito H,Takeda Y,Danura T,Kass IS,Morita Kdoi
10.1016/j.neuroscience.2013.01.010subject
Has Abstractpub_date
2013-04-03 00:00:00pages
59-69eissn
0306-4522issn
1873-7544pii
S0306-4522(13)00035-3journal_volume
235pub_type
杂志文章相关文献
NEUROSCIENCE文献大全abstract::Despite extensive investigations into the mechanisms of aerobic respiration in mitochondria, the spontaneous metabolic activity of individual cells within a whole animal has not been observed in real time. Consequently, little is known about whether and how the level of mitochondrial energy metabolism is regulated in ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2007.12.020
更新日期:2008-03-03 00:00:00
abstract::In rats under urethane anaesthesia gamma-aminobutyric acid agonists and uptake blockers were microiontophoretically applied in the pyramidal layer of CA1 and in the apical dendrites using a twin set of multibarrelled micropipettes. Thus, the somatic and dendritic field potentials elicited by commissural stimulation we...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(84)90072-1
更新日期:1984-06-01 00:00:00
abstract::We have previously shown that the myristoylated alanine-rich C kinase substrate, a primary protein kinase C substrate in brain that binds and cross-links filamentous actin, is enriched in neuronal growth cones and is developmentally regulated in brain. Here we examined myristoylated alanine-rich C kinase substrate exp...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(00)00039-7
更新日期:2000-01-01 00:00:00
abstract::Both long-term depression and long-term potentiation have been described at corticostriatal synapses. These long-lasting changes in synaptic strength were classically induced by high-frequency (100 Hz) electrical stimulations of cortical afferents. The purpose of the present study was to test the ability of corticostr...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(98)00719-2
更新日期:1999-01-01 00:00:00
abstract::In IMR32 neuroblastoma cells, the two adenosine receptor agonists N6-R-phenylisopropyladenosine and 5'-N-ethylcarboxamidoadenosine dose-dependently stimulated membrane adenylate cyclase activity with potencies consistent with the presence of adenosine receptors of the A2-subtype. The S enantiomer of N6-R-phenylisoprop...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(89)90173-5
更新日期:1989-01-01 00:00:00
abstract::The aim of the present study was to determine whether thoracic spinal manipulation (SM) decreases temporal summation of back pain. The study comprised two controlled experiments including 16 and 15 healthy participants, respectively. Each study included six sessions during which painful or non-painful electrical stimu...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2017.03.006
更新日期:2017-05-04 00:00:00
abstract::Monoamine oxidase, monoamine oxidase-A, and monoamine oxidase-B activities were compared in free moving, rapid eye movement sleep-deprived, recovered, and control rat brains. The activities were estimated in the whole brain, cerebrum, cerebellum, whole brainstem, medulla, pons, and midbrain. The flowerpot method was u...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(93)90433-g
更新日期:1993-08-01 00:00:00
abstract::We have investigated a potential mechanism by which a diet, similar in composition to the typical diet of most industrialized western societies rich in saturated fat and refined sugar (HFS), can influence brain structure and function via regulation of neurotrophins. We show that animals that learn a spatial memory tas...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(02)00123-9
更新日期:2002-01-01 00:00:00
abstract::In the adult CNS, tissue-specific germinal niches, such as the subventricular zone of the lateral ventricles and the subgranular zone of the dentate gyrus of the hippocampus, contain multipotent neural precursor cells (NPCs) with the capacity to self-renew and differentiate into functional brain cells (i.e. neurons, a...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2013.07.022
更新日期:2013-10-10 00:00:00
abstract::Brain injury is associated with neuroinflammation, and microglia are key players in this process. Microglia can acquire pro-inflammatory or anti-inflammatory properties, but how this affects neural stem cells (NSCs) remains controversial. Here, NSCs were grown in conditioned media collected from either non-stimulated ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2017.10.034
更新日期:2019-05-01 00:00:00
abstract::The posterior parahippocampal gyrus (PPHG) of the non-human primate brain has a distinct dual role in cortical neural systems. On the one hand, it is a critical link in providing the entorhinal cortex and hippocampal formation with cortical input, while on the other hand it receives output from these structures and pr...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2008.03.077
更新日期:2008-06-23 00:00:00
abstract::Increasing evidence indicates that both the ATP P2X receptors and adrenergic systems play a very important role in the development of nociception. However, there is little information concerning the interactions between these two systems in the dorsal root ganglia (DRG). In the present study, we examined the effects o...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2010.12.048
更新日期:2011-03-10 00:00:00
abstract::Examination of spontaneous miniature endplate potentials (MEPPs) in murine skeletal muscle has revealed that in conditions such as botulinum poisoning, during nerve terminal regeneration or in the presence of the drug 4-aminoquinoline, two types of acetylcholine release are responsible for the MEPPs. In addition to th...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(83)90041-6
更新日期:1983-05-01 00:00:00
abstract::Central oxytocin plays an important role in regulating emotionality. The amygdala expresses gonadal steroid-sensitive oxytocin binding sites in both the central and medial sub-nuclei, although the densities markedly differ between these nuclei. These studies examined the in vitro electrophysiological effects of oxytoc...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2005.04.004
更新日期:2005-01-01 00:00:00
abstract::The effects of electrical stimulation of the hypothalamus paraventricular nucleus on the spontaneous firing of mitral and granule cells in the main olfactory bulb were examined in ovariectomized female rats under urethane anaesthesia. High-frequency stimulation (0.5-1.0 mA, 10-20 pulses at 100 Hz) of the paraventricul...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(95)00599-4
更新日期:1996-06-01 00:00:00
abstract::Interactions between neurotransmitters and the immune system represent new prospects for understanding neuroinflammation and associated neurological disease. GABA is the chief inhibitory neurotransmitter but its actions on immune pathways in the brain are unclear. In the present study, we investigated GABAergic transp...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2014.04.037
更新日期:2014-07-25 00:00:00
abstract::Organotypic cultures established from the third thoracic ganglion of locust embryo have been used to investigate dopamine receptors. In this in vitro system, neurites emerge directly from the explants and form a dense network around the explants, presenting cell surface freely exposed for experimental labelling. Polyc...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(94)90088-4
更新日期:1994-02-01 00:00:00
abstract::The modulation of neurotransmitter release by calcium channels is well established, yet, sodium channels were regarded mainly as charge carriers. Many lines of evidence suggest a more fine-tuning role played by sodium channels. Using rat cerebrocortical isolated nerve endings (synaptosomes) and two toxins that have se...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(02)00139-2
更新日期:2002-01-01 00:00:00
abstract::The responses in T3-4, T10-11 and L2-3 white rami to stimulation of different zones of the bulbar ventrolateral surface were maximal when the region of about 4 mm laterally to the midline was stimulated. A weak surface stimulation of all these zones elicited only a long latency response consisting of three waves. A sh...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(86)90236-8
更新日期:1986-01-01 00:00:00
abstract::We have previously observed that migraine attacks impervious to triptan therapy were readily terminated by subsequent i.v. administration of the non-steroidal anti-inflammatory drug (NSAID) ketorolac. Since such attacks were associated with periorbital allodynia--a symptom of central sensitization--we examined whether...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2007.04.064
更新日期:2007-08-24 00:00:00
abstract::Clinical reports show that oxytocin (OT) is related to stress-related disorders such as depression, anxiety disorder, and post-traumatic stress disorder. Two key structures in the brain should be paid special attention with regard to stress regulation, namely, the hypothalamus and the hippocampus. The former is the re...
journal_title:Neuroscience
pub_type: 杂志文章,评审
doi:10.1016/j.neuroscience.2019.07.046
更新日期:2019-10-01 00:00:00
abstract::The role of brain corticotropin-releasing hormone receptors in modulating hypothalamic-pituitary-adrenal and sympathoadrenal responses to acute immobilization stress was studied in conscious rats under central corticotropin-releasing hormone receptor blockade by intracerebroventricular injection of a peptide corticotr...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(99)00333-4
更新日期:1999-01-01 00:00:00
abstract::Grafted neurons obtained from the fetal basal forebrain can provide a functional cholinergic reinnervation of the hippocampal formation in rats with a lesion of the intrinsic septal cholinergic afferents. In the present experiments graft-derived acetylcholine release in the hippocampus was studied by microdialysis in ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(92)90074-c
更新日期:1992-07-01 00:00:00
abstract::Changes in the amplitude of the calcium inward current caused by intracellular administration of tolbutamide (an inhibitor of the cyclic AMP-dependent protein kinase activity) or catalytic subunits of cAMP-dependent protein kinases from rabbit myocardium were studied on internally perfused nerve cells of the snail, He...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(84)90229-x
更新日期:1984-01-01 00:00:00
abstract::Cholesterol is an essential substance for maintaining normal structure and function of the brain. But unfortunately, a long-term high-cholesterol diet can lead to a variety of pathological changes of the brain such as β-amyloid (Aβ) accumulation, Tau hyperphosphorylation, reactive gliosis, neuroinflammation, neuronal ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2015.12.039
更新日期:2016-03-01 00:00:00
abstract::In rats under continuous stress (CS) there is decreased hypothalamic dopaminergic innervation to the intermediate lobe (IL) of the pituitary gland, which causes hyperactivation and subsequent degeneration of melanotrophs in the IL. In this study, we investigated the molecular basis for the changes that occur in melano...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2010.06.030
更新日期:2010-09-15 00:00:00
abstract::The incidence of asthma is more common in boys than in girls during the childhood, and more common in premenopausal female than age-matched males. Our previous study demonstrated a gender difference in histamine-mediated neuroexcitability in nodose ganglia neurons (NGNs), highlighting a possibility of histamine-mediat...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2015.06.049
更新日期:2015-09-10 00:00:00
abstract::Miniature endplate potentials were recorded from the isolated pectoralis cutaneous muscle of the frog during very stable recording conditions and low noise levels. Two to 5 x 10(3) miniature endplate potentials were filmed at eight unstressed junctions that met rigorous experimental criteria for analysis. Seven juncti...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(87)90093-5
更新日期:1987-11-01 00:00:00
abstract::The effect of re-exposure of rats to an aversive environment on the induction of long-term potentiation was investigated in the CA3 region 3 and 12 h after contextual conditioning. Electro-physiological recordings showed that re-exposure of rats to the conditioning chamber produced a significant and long-lasting decre...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(97)00285-6
更新日期:1998-01-01 00:00:00
abstract::Large-scale transcriptome analysis in the brain is a powerful approach to identify novel genes of potential interest toward understanding cerebral organization and function. We utilized the microarray technology to measure expression levels of about 24,000 genes and expressed sequence tags in mouse hippocampus, fronta...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2007.02.040
更新日期:2007-05-25 00:00:00