Abstract：
:Increasing evidence indicates that both the ATP P2X receptors and adrenergic systems play a very important role in the development of nociception. However, there is little information concerning the interactions between these two systems in the dorsal root ganglia (DRG). In the present study, we examined the effects of noradrenaline (NA) on the P2X3 receptor expression in the DRG of Sprague-Dawley rats. The direct effect of NA on the P2X3 mRNA and protein expression was determined in cultured DRG neurons. Treatment of neuronal cultures with NA (50 nM, 5 h) induced a two-fold increase in P2X3 expression, as detected with real-time RT-PCR and Western blots, but had no effect on P2X2 expression. In electrophysiological experiments, perfusion of neuronal cultures with the P2X3 agonist (αβ-methylene ATP) increased neuronal firing rate by 139% and 273% in neurons treated with either PBS (control) or NA, respectively, indicating that chronic NA treatment significantly enhanced the neuronal response to P2X3 activation. In behavior studies, combination of NA (2 or 20 nmol) with αβ-methylene ATP (10 nmol) produced a significant and long lasting augmentation of thermal hyperalgesia. These results indicate that NA stimulates P2X3 expression in DRG neurons, and this could contribute to the development of pain sensitization.
journal_name
Neurosciencejournal_title
Neuroscienceauthors
Tan Y,Sun L,Zhang Qdoi
10.1016/j.neuroscience.2010.12.048subject
Has Abstractpub_date
2011-03-10 00:00:00pages
32-8eissn
0306-4522issn
1873-7544pii
S0306-4522(10)01653-2journal_volume
176pub_type
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