Abstract:
:Extracellular adenosine 5'-triphosphate (eATP) acts as a neurotransmitter within the retina and brain, activating a range of ionotropic P2X and metabotropic P2Y receptors. In this study, the specific localization of the P2X4 receptor (P2X4-R) subunit was evaluated in the retina using fluorescence immunohistochemistry and pre-embedding immuno-electron microscopy. Punctate P2X4-R labeling was largely localized to the inner and outer plexiform layers of mouse, rat and cat retinae. In the mouse outer retina, double-labeling of P2X4-R with the horizontal cell marker, calbindin, revealed P2X4-R immunoreactivity (P2X4-R-IR) on horizontal cell somata and processes. In the inner retina, P2X4-R expression was found closely associated with rod and cone bipolar cell terminals, and the punctate labeling was observed on calretinin-positive amacrine cells. Using immuno-electron microscopy, P2X4-Rs were observed on processes post-synaptic to photoreceptor and bipolar cell terminals, likely representing horizontal, amacrine and ganglion cells, respectively. Furthermore, P2X4-R expression was also observed on Müller cells, astrocytes and microglia. These data suggest a role for P2X4-Rs in the lateral inhibitory pathways of the retina, modulating neuronal function of photoreceptors and bipolar cells. The expression on macro- and microglial cells implicates a role for P2X4-Rs in glial signaling, tissue homeostasis and immunosurveillance within the mammalian retina.
journal_name
Neurosciencejournal_title
Neuroscienceauthors
Ho T,Vessey KA,Fletcher ELdoi
10.1016/j.neuroscience.2014.06.055subject
Has Abstractpub_date
2014-09-26 00:00:00pages
55-71eissn
0306-4522issn
1873-7544pii
S0306-4522(14)00540-5journal_volume
277pub_type
杂志文章相关文献
NEUROSCIENCE文献大全abstract::Spontaneous activity in medial vestibular nucleus (MVN) neurons is modulated by synaptic inputs. These inputs are crucial for maintaining gaze and posture and contribute to vestibular compensation after lesions of peripheral vestibular organs. We investigated how chronically attenuated glycinergic input affects excita...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2010.06.040
更新日期:2010-09-29 00:00:00
abstract::The mechanical response of human m. flexor pollicis longus to slow (3.2 degrees/s) linear stretch by 5.5 degrees was measured during sustained (45-60 s, 9-13.5 p.p.s.) unfused tetanus evoked by electrical stimulation. The stiffness increased during unfused tetanus. At the late phase of unfused tetanus it was 1.8 +/- 0...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(97)00130-9
更新日期:1997-09-01 00:00:00
abstract::Neuronal activity associated with visual processing of an unfamiliar face gradually diminishes when it is viewed repeatedly. This process, known as repetition suppression (RS), is involved in the acquisition of familiarity. Current models suggest that RS results from interactions between visual information processing ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2016.03.059
更新日期:2016-06-21 00:00:00
abstract::Monoclonal antibodies to rat striatal choline acetyltransferase were produced by fusion of sensitized mouse lymphocytes with murine plasmacytoma (NS1) cells. Two stable anti-choline acetyltransferase lines were established by limiting dilution cloning. Specificity of antibody was established by the following criteria:...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(83)90229-4
更新日期:1983-11-01 00:00:00
abstract::Neuromedin U is a newly described regulatory peptide, found by radioimmunoassay in significant concentrations in both the brain and gut of the rat. The aim of the present study was to localize this peptide immunoreactivity to discrete structures of the gut and brain and to map its distribution using immunocytochemistr...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(88)90037-1
更新日期:1988-06-01 00:00:00
abstract::Cerebral ischemia is the most common cause of stroke with high morbidity, disability and mortality. Sirtuin-2 (Sirt2), a vitally important NAD+-dependent deacetylase which has been widely researched in central nervous system diseases, has also been identified as a promising treatment target using its specific inhibito...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2018.01.040
更新日期:2018-03-15 00:00:00
abstract::The effects of centrifugal afferents on membrane potentials of identified granule cell layer using evoked field potential profiles, and trans-synaptic activation via antidromic stimulation of output cell axon collaterals. Intracellular recordings maintained for 4-30 min showed complex spontaneous spike discharges and ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(92)90496-o
更新日期:1992-01-01 00:00:00
abstract::Synaptic plasticity involves remodeling of extracellular matrix. This is mediated, in part, by enzymes of the matrix metalloproteinase (MMP) family, in particular by gelatinase MMP-9. Accordingly, there is a need of developing methods to visualize gelatinolytic activity at the level of individual synapses, especially ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2008.05.045
更新日期:2009-01-12 00:00:00
abstract::Neurosteroids such as dehydroepiandrosterone (DHEA), pregnenolone and 17beta-estradiol are synthesized by cytochrome P450s from endogenous cholesterol. We previously reported a new cytochrome P450 enzyme, CYP7B, highly expressed in rat and mouse brain that metabolizes DHEA and related steroids by hydroxylation at the ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(03)00438-x
更新日期:2003-01-01 00:00:00
abstract::Agrin, a synaptic basal lamina protein, is essential for the formation of the vertebrate neuromuscular junction. Agrin's role in synaptogenesis in the central nervous system has, however, not been elucidated. Therefore, we performed immunohistochemical analysis of agrin localization in adult rat brain using agrin-spec...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(96)00345-4
更新日期:1997-01-01 00:00:00
abstract::Several lines of research have implicated glutathione (GSH) in schizophrenia. For instance, GSH deficiency has been reported in the prefrontal cortex of schizophrenics in vivo. Further, in rats postnatal GSH-deficiency combined with hyperdopaminergia led to cognitive impairments in the adult. In the present report we ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2005.01.059
更新日期:2005-01-01 00:00:00
abstract::Our series of rat experiments have shown that locomotor activity, arousal level, body and brown adipose tissue temperatures, heart rate and arterial pressure increase episodically in an integrated manner approximately every 100min (ultradian manner). Although it has been proposed that the integrated ultradian pattern ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2016.07.041
更新日期:2016-10-15 00:00:00
abstract::A previous study investigating potential adult hippocampal neurogenesis in microchiropteran bats failed to reveal a strong presence of this neural trait. As microchiropterans have a high field metabolic rate and a small body mass, it is possible that capture/handling stress may lead to a decrease in the detectable pre...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2014.07.063
更新日期:2014-09-26 00:00:00
abstract::A significant number of postmenopausal women report increased anxiety and vulnerability to stress, which has been linked to decreased secretion of ovarian steroids. Communication between the serotonin system and the corticotropin releasing factor (CRF) system determines stress sensitivity or resilience. This study exa...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2010.08.059
更新日期:2010-12-15 00:00:00
abstract::We have examined the effect of acrolein, an aldehyde product of lipid peroxidation, on axons in isolated guinea-pig spinal cord white matter. We found that 200 microM acrolein, but not 50 microM, induced a time-dependent loss of compound action potential conduction. Such conduction loss was irreversible within 1 h aft...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(02)00457-8
更新日期:2002-01-01 00:00:00
abstract::Amyloid-β (Aβ) production and clearance in the brain is a crucial focus of investigations into the pathogenesis of Alzheimer disease. Imbalance between production and clearance leads to accumulation of Aβ. The important Aβ-degrading enzymes in the brain are neprilysin (NEP) and insulin-degrading enzyme (IDE), and defe...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2017.08.030
更新日期:2017-10-24 00:00:00
abstract::Deafferentation of the adult rat dentate gyrus induces reactive axonal growth by surviving afferent systems. In middle aged and aged rats, axonal sprouting is delayed and reduced relative to young adults. The cause for this age-related decline is not known, but it may reflect a decrement in trophic signals which initi...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(97)00539-3
更新日期:1998-04-01 00:00:00
abstract::Rapid changes in the light-dark cycle cause circadian desynchronization between rhythms of spike-wave discharges (SWDs) and motor activity in genetic epileptic rats, and this is accompanied by an increase in epileptic activity. Given the close relationship between absence seizures and sleep-wake states, the present st...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2019.03.062
更新日期:2019-06-01 00:00:00
abstract::During a critical period in its postnatal development the mammalian visual cortex displays susceptibility to experience-dependent alterations of neuronal response properties. Plasticity represents an integrated set of developmental processes controlled by a transcriptional hierarchy that coordinates the action of many...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(01)00570-x
更新日期:2002-01-01 00:00:00
abstract::There is evidence of structural and functional deterioration in the brain, including the prefrontal cortex (PFC) and hippocampus, during the normal aging process in animals and humans. Extracellular matrix-associated glycoproteins, such as chondroitin sulfate proteoglycans (CSPGs), are involved in not only maintaining...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2009.08.060
更新日期:2009-12-15 00:00:00
abstract::Methyl-CpG-binding protein 2 is a characteristic member of the methyl-CpG-binding protein family of transcription regulators. In conjunction with Sin3, MeCP2 recruits class I histone deacetylases to methyl-CpG regions to suppress transcription. Rett syndrome, a disorder characterized by mental retardation and autistic...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(02)00586-9
更新日期:2003-01-01 00:00:00
abstract::Studies demonstrated that chronic high-dose homocysteine administration induced learning and memory impairment in animals. Atractylenolide III (Aen-III), a neuroprotective constituent of Atractylodis macrocephalae Koidz, was isolated in our previous study. In this study, we investigated potential benefits of Aen-III i...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2015.01.060
更新日期:2015-04-02 00:00:00
abstract::We here investigated the effects of neonatal lesions of the entorhinal cortex (EC) in rats on maze learning and on structural alterations of its main projection region, the hippocampus, as well as other regions with anatomical connections to the EC that are involved in maze learning. Since early brain damage is consid...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2008.03.017
更新日期:2008-06-02 00:00:00
abstract::Activation of neurokinin-1 (NK-1) receptors in the rostral ventromedial medulla (RVM) can facilitate pain transmission in conditions such as inflammation, and thereby contribute to hyperalgesia. Since blockade of NK-1 receptors in the RVM can attenuate hyperalgesia produced by prolonged inflammation, we examined the r...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2012.08.029
更新日期:2012-11-08 00:00:00
abstract::Immunohistochemistry for vanilloid receptor 1-like receptor (VRL-1), a candidate transducer for high-threshold noxious heat, was performed on rat trigeminal primary sensory neurons. The immunoreactivity was detected in 14% of the trigeminal ganglion cell bodies, while the neurons in the mesencephalic trigeminal tract ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(00)00427-9
更新日期:2000-01-01 00:00:00
abstract::The axonal transport of the molecular forms of acetylcholinesterase was investigated in regenerating facial nerves of guinea-pig and rat. Four forms were separated by velocity sedimentation corresponding to 16S (A12), 10S (G4), 6S (G2) and 4S (G1) acetylcholinesterase. They displayed species-specific changes, which ar...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(88)90365-x
更新日期:1988-02-01 00:00:00
abstract::Antagonists acting at the 5-hydroxytryptamine3 receptor are potent anti-emetic agents in cases of cytotoxic- and radiation-induced vomiting, and binding sites for these compounds have been described in brainstem areas known to be involved in mediation of nausea and vomiting. We have used autoradiography to examine the...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(90)90060-h
更新日期:1990-01-01 00:00:00
abstract::N-Methyl-D-aspartate receptors are thought to be involved in synaptic signaling within the hypothalamo-neurohypophysial system, but the extent and nature of their involvement has not been determined. In this study, in the rat, we evaluated the effect of hyperosmotic stimulation on the N-methyl-D-aspartate receptor sub...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(96)00544-1
更新日期:1997-05-01 00:00:00
abstract::The present studies aimed to determine whether estradiol (E(2)) modulates the stimulation of cocaine- and amphetamine-regulated transcript (CART) peptide in the mesolimbic and nigrostriatal dopaminergic systems. I.c.v. administration of the CART peptide (55-102, 1 microg/3 microl) increased dopamine turnover (3,4-dihy...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2008.01.086
更新日期:2008-07-17 00:00:00
abstract::This good laboratory practice (GLP) study of aluminum salts in Sprague-Dawley rats was conducted according to double-blind, vehicle-controlled randomized design by exposing offspring to aluminum citrate in-utero, through lactation, and then in drinking water post-weaning. Three dose levels were used: 30, 100, 300 mg A...
journal_title:Neuroscience
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.neuroscience.2011.05.008
更新日期:2011-10-13 00:00:00