Abstract:
:In IMR32 neuroblastoma cells, the two adenosine receptor agonists N6-R-phenylisopropyladenosine and 5'-N-ethylcarboxamidoadenosine dose-dependently stimulated membrane adenylate cyclase activity with potencies consistent with the presence of adenosine receptors of the A2-subtype. The S enantiomer of N6-R-phenylisopropyladenosine induced a significantly lower stimulation of adenylate cyclase, accordingly to its lower ability to activate adenosine receptors. These effects were selectively counteracted by the adenosine receptor antagonist theophylline and, conversely, were not affected by the A1-adenosine receptor selective blocker 8-cyclopentyl-1,3-dipropylxanthine. No adenosine receptors belonging to the A1-subtype seem, therefore, to be present in this cell line, as also shown by the lack of inhibitory activity of N6-R-phenylisopropyladenosine on both basal and forskolin-stimulated adenylate cyclase activity. Activation of A2-receptors did not modify intracellular basal calcium levels, did not influence calcium influx through voltage-dependent calcium channels and did not modify calcium influx and redistribution induced by muscarinic receptor activation. Prolonged exposure of cells to either N6-R-phenylisopropyladenosine or 5'-N-ethylcarboxamidoadenosine was associated with a small but significant degree of morphological differentiation, comparable to that induced by dibutyryl cAMP, and therefore presumably related to the prolonged increase of intracellular cAMP levels elicited by the two adenosine agonists. After cellular differentiation induced with either dibutyryl cAMP or 5-bromodeoxyuridine, a selective desensitization of A2-receptor stimulated adenylate cyclase activity was found.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Neurosciencejournal_title
Neuroscienceauthors
Abbracchio MP,Cattabeni F,Clementi F,Sher Edoi
10.1016/0306-4522(89)90173-5subject
Has Abstractpub_date
1989-01-01 00:00:00pages
819-25issue
3eissn
0306-4522issn
1873-7544pii
0306-4522(89)90173-5journal_volume
30pub_type
杂志文章相关文献
NEUROSCIENCE文献大全abstract::The consequences of epileptiform discharge on the expression of glutamate and GABA receptors were examined by in situ hybridization histochemistry after treatment of rat hippocampal slice cultures with convulsants. Application of 500 microM picrotoxin for two days led to decreases in the messenger RNA levels for the N...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(95)00130-b
更新日期:1995-08-01 00:00:00
abstract::Synapsins (SynI, SynII, SynIII) are a multigene family of synaptic vesicle (SV) phosphoproteins implicated in the regulation of synaptic transmission and plasticity. Synapsin I, II, I/II and I/II/III knockout mice are epileptic and SYN1/2 genes have been identified as major epilepsy susceptibility genes in humans. We ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2010.08.046
更新日期:2010-11-24 00:00:00
abstract::Adrenalectomy (ADX) abolishes behavioral sensitization to cocaine in DBA/2, but not C57BL/6 inbred mice. The present study tests the hypothesis that this ADX effect on behavioral sensitization in the DBA/2 strain involves changes in midbrain dopamine systems that do not occur in the C57BL/6 strain. For that purpose, w...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2008.03.004
更新日期:2008-05-15 00:00:00
abstract::I have prepared a number of monoclonal antibodies to chromaffin cell membranes. One of these antibodies recognizes a number of antigenically related proteins that are present in all tissues examined. In the adrenal, these proteins are completely excluded from chromaffin granules but are present in other subcellular me...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(87)90204-1
更新日期:1987-07-01 00:00:00
abstract::Leptin and somatostatin (SRIF) have opposite effects on food seeking and ingestive behaviors, functions partially regulated by the frontoparietal cortex and hippocampus. Although it is known that the acute suppression of food intake mediated by leptin decreases with time, the counter-regulatory mechanisms remain uncle...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2013.08.019
更新日期:2013-11-12 00:00:00
abstract::Efferent projections of intrastriatally implanted striatal neurons have been studied using a combination of anterograde and retrograde axonal tracers. Adult rats subjected to a unilateral ibotenic acid lesion of the head of the caudate putamen received cell suspension grafts obtained from 14 15-day-old striatal primor...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(89)90256-x
更新日期:1989-01-01 00:00:00
abstract::Autism is a neurodevelopmental disorder characterized by abnormal reciprocal social interactions, communication deficits, and repetitive behaviors with restricted interests. BTBR T+tf/J (BTBR) is an inbred mouse strain that displays robust behavioral phenotypes with analogies to all three of the diagnostic symptoms of...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2010.09.059
更新日期:2010-12-29 00:00:00
abstract::Although multiple effects of GABA(B) receptor activation on synaptic transmission in the striatum have been described, the precise locations of the receptors mediating these effects have not been determined. To address this issue, we carried out pre-embedding immunogold electron microscopy in the rat using antibodies ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2005.07.013
更新日期:2005-01-01 00:00:00
abstract::Arc (also termed activity-regulated cytoskeleton-associated protein or Arg3.1), is an effector immediate early gene whose upregulation has been demonstrated during events of synaptic plasticity. In the present study, the possibility that Arc would be specifically upregulated in rats during the acquisition of a quickly...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(01)00605-4
更新日期:2002-01-01 00:00:00
abstract::Direct intracerebral administration of N-methyl-D-aspartate typically produces focal brain injury. (+)-5-Methyl-10,11-dihydro-5H-dibenzo[a,d]cyclohepten-5,10-immi ne maleate (MK-801), a non-competitive N-methyl-D-aspartate antagonist, can protect against N-methyl-D-aspartate-mediated brain injury when administered sho...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(90)90377-g
更新日期:1990-01-01 00:00:00
abstract::The human brain is composed of complex networks of 100 billion neurons that underlie its higher functions. The set of neural connections in the brain has recently attracted growing interest from the scientific community. It is important to identify individual differences in these neural connections to study the backgr...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2015.06.032
更新日期:2015-08-20 00:00:00
abstract::Gap junctional intercellular communication (GJIC) may play an important role in the hearing process. Cisplatin is an anticancer drug that causes hearing loss and Gingko biloba extracts (EGb 761) have been used as an antioxidant and enhancer for GJIC. The purpose of this study was to examine the efficiency of EGb 761 i...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2013.04.001
更新日期:2013-08-06 00:00:00
abstract::Migration of neurons is a key step in the formation of the central nervous system, and an increase in internal Ca(2+) concentration has been shown to increase the rate of migration of granule cells along radial glial processes in slices of postnatal cerebellum. In embryonic spinal cord, the non-radial migration of aut...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(00)00070-1
更新日期:2000-01-01 00:00:00
abstract::The in vivo effects of [D-Pen2,D-Pen5]enkephalin, a cyclic peptide agonist with high affinity and selectivity for the delta opioid receptors, on the endogenous aluminum content of selected areas of rat brain and spinal cord were studied by means of atomic absorption spectrophotometry. Intracerebroventricular injection...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(94)00575-p
更新日期:1995-05-01 00:00:00
abstract::Development of functional synapses is crucial for the transmission and storage of information in the brain. Post establishment of the initial synaptic contact, synapses are stabilized through neuronal activity-induced signals. Emerging studies have implicated ubiquitination; a reversible posttranslational modification...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2017.08.057
更新日期:2017-11-19 00:00:00
abstract::In awake, freely moving rats, the intracerebral administration of the excitatory amino acid L-glutamate (30 nmol/0.5 microliters) into discrete regions of the brainstem resulted in a transient and spontaneous pain-like syndrome characterized by an initial vocalization and vigorous escape behavior. Systematic microinje...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(92)90142-o
更新日期:1992-01-01 00:00:00
abstract::The development of the catecholaminergic system in the hypothalamus and in the septal region was studied in rats from the 12th fetal day until the 9th postnatal day. Catecholaminergic structures were visualized with pre-embedding immunocytochemistry using antiserum to tyrosine hydroxylase. An intensification of diamin...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(89)90338-2
更新日期:1989-01-01 00:00:00
abstract::The piriform cortex is thought to be involved in temporal lobe seizure propagation, such as that occurring during kindling of the amygdala or hippocampus. A number of observations suggested that the circuits of the piriform cortex might act as a critical pathway for limbic seizure discharges to assess motor systems, b...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(00)00407-3
更新日期:2000-01-01 00:00:00
abstract::The possible involvement of fibroblast growth factor receptor (FGFR) activation in the dorsal root ganglion (DRG) was examined following peripheral nerve injury in the rat. Ligation of the sciatic nerve down-regulated FGFR2, -3 and -4 mRNA; however, the expression of FGFR1 mRNA showed no change. Activation of FGFR was...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2007.08.024
更新日期:2007-11-30 00:00:00
abstract::The senses of hearing and balance in vertebrates are transduced by hair cells in the inner ear. Hair cells from a wide variety of organisms have been described electrophysiologically but this is the first report of the application of these techniques to the genetically tractable zebrafish model system. Auditory and ve...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2013.05.062
更新日期:2013-09-17 00:00:00
abstract::Huntington's disease is a progressive, autosomal dominantly inherited, neurodegenerative disease that is characterized by involuntary movements (chorea), cognitive decline and psychiatric manifestations. This is one of a number of late-onset neurodegenerative disorders caused by expanded glutamine repeats, with a like...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(00)00391-2
更新日期:2000-01-01 00:00:00
abstract::Throughout rat embryogenesis we analysed the expression patterns of the three mature transcripts generated from the two calcitonin gene-related peptide genes: calcitonin, alpha-calcitonin gene-related peptide, and beta-calcitonin gene-related peptide messenger RNAs. In addition, we examined in parallel the distributio...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(98)00730-1
更新日期:1999-01-01 00:00:00
abstract::Electrophysiological and anatomical studies were carried out in parallel to investigate the ability of lateral geniculate body neurons to regenerate axons damaged by the removal of the primary visual cortex and to innervate graft neurons functionally after transplantation of fetal neocortical tissue to a lesion cavity...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(94)90278-x
更新日期:1994-06-01 00:00:00
abstract::GABA(B) receptors at the cone terminals in bullfrog retina were characterized by immunocytochemical and whole-cell patch clamp techniques in retinal slice preparations. Somata, axons and synaptic terminals (pedicles) of cones were both GABA(B) receptor (GABA(B)R) 1 and GABA(B)R2 immunoreactive. Physiologically, barium...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2004.12.024
更新日期:2005-01-01 00:00:00
abstract::In the present study we have examined the topographic and temporal patterns of expression of the limbic system associated membrane protein by light and electron microscopic immunocytochemistry in organotypic cultures of the rat brain. The regional, cellular and subcellular distribution of staining in young cultures wa...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(89)90192-9
更新日期:1989-01-01 00:00:00
abstract::The serotonin and circadian systems are principal regulatory networks of the brain. Each consists of a unique set of neurons that make widespread neural connections and a defined gene network of transcriptional regulators and signaling genes that subserve serotonergic and circadian function at the genetic level. These...
journal_title:Neuroscience
pub_type: 杂志文章,评审
doi:10.1016/j.neuroscience.2011.09.036
更新日期:2011-12-01 00:00:00
abstract::Amyotrophic Lateral Sclerosis (ALS) is a neurodegenerative disease that affects upper and lower motor neurons and leads to death a few years after symptom onset. Despite its high morbidity and mortality, its underlying pathogenic mechanisms still remain poorly understood. Although there is increasing evidence for sign...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2020.07.047
更新日期:2020-12-01 00:00:00
abstract::The study explored unintentional force changes elicited by removing visual feedback during cyclical, two-finger isometric force production tasks. Subjects performed two types of tasks at 1Hz, paced by an auditory metronome. One - Force task - required cyclical changes in total force while maintaining the sharing, defi...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2017.03.022
更新日期:2017-05-14 00:00:00
abstract::Wnt proteins have been implicated in regulating a variety of developmental processes in the CNS. Secreted Frizzled-related protein 3 (sFRP3) is a member of the sFRP family that can inhibit the Wnt signaling by binding directly to Wnts via their regions of homology to the Wnt-binding domain of Frizzleds. Recent studies...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2013.06.044
更新日期:2013-09-17 00:00:00
abstract::This study evaluated the protective role of p38 mitogen-activated protein kinase (p38 MAPK) inhibitors and sequestosome 1 (Sqstm1/A170/p62), a stress-induced signal modulator, in acoustic injury of the cochlea in mice. Two weeks after the exposure of mice to acoustic stress, threshold shifts of the auditory brainstem ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2009.12.038
更新日期:2010-03-17 00:00:00