DNA "fingerprinting" reveals high levels of inbreeding in colonies of the eusocial naked mole-rat.

abstract：:The synaptic vesicle membrane protein synaptotagmin (tagmin) is essential for fast, calcium-dependent, neurotransmitter release and is likely to be the calcium sensor for exocytosis, because of its many calcium-dependent properties. Polyphosphoinositides are needed for exocytosis, but it has not been known why. We now...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schiavo G,Gu QM,Prestwich GD,Söllner TH,Rothman JE

更新日期：1996-11-12 00:00:00

abstract：:The cellular models generally used in the in vitro evaluation of anti-human immunodeficiency virus compounds are dividing cells. A model constituted by resting lymphocytes may more accurately reflect a drug's future efficacy in humans, since viral DNA synthesis is known to take place in quiescent cells, creating a res...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Malley SD,Grange JM,Hamedi-Sangsari F,Vila JR

更新日期：1994-11-08 00:00:00

abstract：:Cell proliferation is regulated by the induction of growth promoting genes and the suppression of growth inhibitory genes. Malignant growth can result from the altered balance of expression of these genes in favor of cell proliferation. Induction of the transcription factor, c-Myc, promotes cell proliferation and tran...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lee TC,Li L,Philipson L,Ziff EB

更新日期：1997-11-25 00:00:00

abstract：:Amyotrophic lateral sclerosis (ALS) is a heterogeneous degenerative motor neuron disease linked to numerous genetic mutations in apparently unrelated proteins. These proteins, including SOD1, TDP-43, and FUS, are highly aggregation-prone and form a variety of intracellular inclusion bodies that are characteristic of d...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ciryam P,Lambert-Smith IA,Bean DM,Freer R,Cid F,Tartaglia GG,Saunders DN,Wilson MR,Oliver SG,Morimoto RI,Dobson CM,Vendruscolo M,Favrin G,Yerbury JJ

更新日期：2017-05-16 00:00:00

abstract：:We report here the reconstitution of a pathway that leads to the apoptotic changes in nuclei by using recombinant DNA fragmentation factor (DFF), a heterodimeric protein of 40 and 45 kDa. Coexpression of DFF40 and DFF45 is required to generate recombinant DFF, which becomes activated when DFF45 is cleaved by caspase-3...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu X,Li P,Widlak P,Zou H,Luo X,Garrard WT,Wang X

更新日期：1998-07-21 00:00:00

abstract：:Reverse transcriptase (RNA-directed DNA polymerase, EC 2.7.7.49) of human immunodeficiency virus type 1 has been examined with respect to the steady-state kinetics of polymerization of dNTPs into product DNA. With dNTPs as variable substrate, the kinetics of polymerization deviated from standard Michaelis-Menten kinet...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： West AB,Roberts TM,Kolodner RD

更新日期：1992-10-15 00:00:00

abstract：:The Etruscans, the only preclassical European population that has been genetically characterized so far, share only two haplotypes with their modern geographic counterparts, the Tuscans, who, nonetheless, appear to be their closest relatives. We modeled 10 demographic scenarios spanning the last 2,500 years and tested...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Belle EM,Ramakrishnan U,Mountain JL,Barbujani G

更新日期：2006-05-23 00:00:00

abstract：:Cytochrome c oxidase (COX) is a 13-subunit protein complex that catalyzes the last step in mitochondrial electron transfer in mammals. Of the 10 subunits encoded by nuclear DNA (three are mtDNA products), some are expressed as tissue- and/or development-specific isoforms. For COX subunit VIII, previous work showed tha...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Goldberg A,Wildman DE,Schmidt TR,Huttemann M,Goodman M,Weiss ML,Grossman LI

更新日期：2003-05-13 00:00:00

abstract：:The solution conformations of 2-substituted derivatives of 1-(beta-D-ribofuranosyl)benzimidazole have been determined by circular dichroism spectroscopy in aqueous solutions. It is shown that analogs with methyl, amino, or methylamino substituents at position 2 of the benzimidazole ring (position 8 of the purine ring)...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miles DW,Townsend LB,Miles DL,Eyring H

更新日期：1979-02-01 00:00:00

abstract：:We have studied the effects of food restriction (FR) and substitution of fish oil (FO; omega 3) for corn oil (CO; omega 6) on breast tumor incidence and survival in mouse mammary tumor virus/v-Ha-ras transgenic (Onco) mice. The diets were as follows: group 1, 5% (wt/wt) CO fed ad libitum (AL); group 2, 5% CO, restrict...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fernandes G,Chandrasekar B,Troyer DA,Venkatraman JT,Good RA

更新日期：1995-07-03 00:00:00

abstract：:Inadequate target exposure is a major cause of high attrition in drug discovery. Here, we show that a label-free method for quantifying the intracellular bioavailability (Fic) of drug molecules predicts drug access to intracellular targets and hence, pharmacological effect. We determined Fic in multiple cellular assay...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mateus A,Gordon LJ,Wayne GJ,Almqvist H,Axelsson H,Seashore-Ludlow B,Treyer A,Matsson P,Lundbäck T,West A,Hann MM,Artursson P

更新日期：2017-07-25 00:00:00

abstract：:Without a fully developed initial segment, the most proximal region of the epididymis, male infertility results. Therefore, it is important to understand the development and regulation of this crucial region. In addition to distinctively high activity levels of the components of the ERK pathway, which are essential fo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xu B,Washington AM,Hinton BT

更新日期：2014-12-30 00:00:00

abstract：:NGC 7027 shows the richest spectrum of any known gaseous nebula. In the region of the spectrum surveyed we have measured lines of ions of H, He, C, N, O, F, Ne, Mg, Si, P, S, Cl, Ar, K, Ca, Mn, and Fe using the new Hamilton Echelle Spectrograph of Lick Observatory with a charge-coupled device (CCD) detector. Since a C...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Aller LH,Keyes CD

更新日期：1988-04-01 00:00:00

abstract：:Band 3, the major intrinsic protein of the human erythrocyte membrane, was specifically labeled with the covalent fluorescent probe eosin isothiocyanate. The lateral mobility of labeled band 3 in the plane of the membrane under various conditions of ionic strength and temperature was examined by using the fluorescence...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Golan DE,Veatch W

更新日期：1980-05-01 00:00:00

abstract：:Supported metal catalysts are extensively used in industrial and environmental applications. To improve their performance, it is crucial to identify the most active sites. This identification is, however, made challenging by the presence of a large number of potential surface structures that complicate such an assignm...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yang AC,Choksi T,Streibel V,Aljama H,Wrasman CJ,Roling LT,Goodman ED,Thomas D,Bare SR,Sánchez-Carrera RS,Schäfer A,Li Y,Abild-Pedersen F,Cargnello M

更新日期：2020-06-30 00:00:00

abstract：:Substance P prohormones were identified in the caudate nucleus, hypothalamus, and substantia nigra of human brain. A polypeptide fraction of acidic brain extracts was fractionated on Sephadex G-50. The lyophilized fractions were sequentially treated with trypsin and a substance P-degrading enzyme with strong preferenc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nyberg F,le Grevés P,Terenius L

更新日期：1985-06-01 00:00:00

abstract：:Mice prefer to mate with individuals expressing different MHC genes from their own. Volatile components presenting MHC-dependent odor types are present in urine and can be detected by mice, as shown by extensive behavioral studies. Similar odor types are suspected to influence human behavior as well. Although a recent...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Montag S,Frank M,Ulmer H,Wernet D,Göpel W,Rammensee HG

更新日期：2001-07-31 00:00:00

abstract：:Repetitive sequences are hotspots of evolution at multiple levels. However, due to difficulties involved in their assembly and analysis, the role of repeats in tumor evolution is poorly understood. We developed a rigorous motif-based methodology to quantify variations in the repeat content, beyond microsatellites, in ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Persi E,Prandi D,Wolf YI,Pozniak Y,Barnabas GD,Levanon K,Barshack I,Barbieri C,Gasperini P,Beltran H,Faltas BM,Rubin MA,Geiger T,Koonin EV,Demichelis F,Horn D

更新日期：2019-08-20 00:00:00

abstract：:During the periimplantation period of pregnancy, pig blastocysts undergo morphological changes and differentiation requiring secretion and transport of nutrients (histotroph) into the uterine lumen. Of these nutrients, glucose is converted to fructose, an isomer of glucose, by conceptus trophectoderm. Although glucose...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kim J,Song G,Wu G,Bazer FW

更新日期：2012-06-19 00:00:00

abstract：:We have recently demonstrated that immunization with hepatitis C virus-like particles (HCV-LPs) generated in insect cells can elicit both humoral and cellular immune responses in BALB/c mice. Here, we evaluate the immunogenicity of HCV-LPs in HLA2.1 transgenic (AAD) mice in comparison to DNA immunization. HCV-LP immun...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Murata K,Lechmann M,Qiao M,Gunji T,Alter HJ,Liang TJ

更新日期：2003-05-27 00:00:00

abstract：:We established a pancreatic adenocarcinoma cell line (CFPAC-1) from a patient with cystic fibrosis (CF) and assessed some of its properties. The cells show epithelial morphology and express cytokeratin and oncofetal antigens characteristic of pancreatic duct cells. Basal and stimulated levels of cAMP and cAMP-dependen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schoumacher RA,Ram J,Iannuzzi MC,Bradbury NA,Wallace RW,Hon CT,Kelly DR,Schmid SM,Gelder FB,Rado TA

更新日期：1990-05-01 00:00:00

abstract：:Glioblastoma multiforme (GBM) is one of the most intractable of human cancers, principally because of the highly infiltrative nature of these neoplasms. Tracking and eradicating infiltrating GBM cells and tumor microsatellites is of utmost importance for the treatment of this devastating disease, yet effective strateg...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jiang X,Fitch S,Wang C,Wilson C,Li J,Grant GA,Yang F

更新日期：2016-11-29 00:00:00

abstract：:Ezrin plays a key role in coupling signal transduction to cortical cell organization. This actin-membrane linker undergoes a series of conformational changes that modulate its interactions with various partners and its localization in membrane or cytosolic pools. Its mobility and exchange rates within and between thes...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Coscoy S,Waharte F,Gautreau A,Martin M,Louvard D,Mangeat P,Arpin M,Amblard F

更新日期：2002-10-01 00:00:00

abstract：:A general and efficient method has been developed to generate large numbers of single-base substitution mutations simply and rapidly. A unique f1 phage recombinant DNA cloning vector is described, which contains the phi X174 origin of viral strand DNA synthesis and allows one to direct mutagenesis to any specific segm...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Abarzúa P,Marians KJ

更新日期：1984-04-01 00:00:00

abstract：:Mammalian thalamus is a critical site where early perception of sensorimotor signals is dynamically regulated by acetylcholine in a behavioral state-dependent manner. In this study, we examined how synaptic transmission is modulated by acetylcholine in auditory thalamus where sensory relay neurons form parallel lemnis...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mooney DM,Zhang L,Basile C,Senatorov VV,Ngsee J,Omar A,Hu B

更新日期：2004-01-06 00:00:00

abstract：:The structure of the NH2-terminal, 20-residue membrane-bound portion of melittin has been computed with empirical energies (ECEPP, Empirical Conformational Energy Program for Peptides). First, a search was made for long stretches of nonpolar residues. Then, the low-energy conformations of these segments were built up ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pincus MR,Klausner RD,Scheraga HA

更新日期：1982-08-01 00:00:00

abstract：:An invariant substructure that forms two interlocked pairs of neighboring beta-strands occurs in essentially all known sandwich-like proteins. Eight conserved positions in these strands were recently shown to act as structural determinants. To test whether the residues at these invariant positions are conserved for me...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wilson CJ,Wittung-Stafshede P

更新日期：2005-03-15 00:00:00

abstract：:Ribozyme activity in vivo depends on achieving high-level expression, intracellular stability, target colocalization, and cleavage site access. At present, target site selection is problematic because of unforeseeable secondary and tertiary RNA structures that prevent cleavage. To overcome this design obstacle, we wis...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Warashina M,Kuwabara T,Kato Y,Sano M,Taira K

更新日期：2001-05-08 00:00:00

abstract：:In this report we examine the effects of chronic anaerobic exposure and subsequent reoxygenation on protein synthesis patterns in Chinese hamster ovary cells. It is observed by two-dimensional gel electrophoresis (isoelectric focusing/NaDodSO4/PAGE) that the transition from an atmospheric environment to an anaerobic s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sciandra JJ,Subjeck JR,Hughes CS

更新日期：1984-08-01 00:00:00

abstract：:Helicobacter hepaticus-infected Rag2(-/-) mice emulate many aspects of human inflammatory bowel disease, including the development of colitis and colon cancer. To elucidate mechanisms of inflammation-induced carcinogenesis, we undertook a comprehensive analysis of histopathology, molecular damage, and gene expression ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mangerich A,Knutson CG,Parry NM,Muthupalani S,Ye W,Prestwich E,Cui L,McFaline JL,Mobley M,Ge Z,Taghizadeh K,Wishnok JS,Wogan GN,Fox JG,Tannenbaum SR,Dedon PC

更新日期：2012-07-03 00:00:00