Abstract:
:Ezrin plays a key role in coupling signal transduction to cortical cell organization. This actin-membrane linker undergoes a series of conformational changes that modulate its interactions with various partners and its localization in membrane or cytosolic pools. Its mobility and exchange rates within and between these two pools were assessed by two-photon fluorescence recovery after photobleaching in epithelial cell microvilli. Analysis of ezrin mutants with an altered actin-binding site revealed three ezrin membrane states of different mobilities and exchange properties, reflecting sequential association with membrane components and F-actin in the context of a fast overall turnover.
journal_name
Proc Natl Acad Sci U S Aauthors
Coscoy S,Waharte F,Gautreau A,Martin M,Louvard D,Mangeat P,Arpin M,Amblard Fdoi
10.1073/pnas.192084599keywords:
subject
Has Abstractpub_date
2002-10-01 00:00:00pages
12813-8issue
20eissn
0027-8424issn
1091-6490pii
192084599journal_volume
99pub_type
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